terclim by ICS banner
IVES 9 IVES Conference Series 9 Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Abstract

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored. Four distinct conditions were analyzed 1) genotype difference with constant light/dark temperature conditions; 2) light vs dark with similar temperature conditions; 3) buds in light (14:00) at >0C vs <0C; 4) buds in dark (6:00 or 18:00) at >0C vs <0C; 4). Principal components analysis indicated that genotype accounted for 66% of variance and there were 1,916 and 1,559 differentially expressed genes (DEG) up and down regulated respectively, in Reisling relative to Cabernet Franc. A greater number of DEG were identified for light relative to dark samples (14:00 vs 6:00 or 18:00) and samples collected at temperatures >0C vs <0C. Gene pathway analysis showed significant positive enrichment in hormone signaling and secondary metabolite pathways in both genotypes in the >0C relative <0C temperature conditions indicating transient temperature changes enhance the metabolic activity of dormant buds.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Prakriti Sharma1, Jason P. Londo2, Anne Fennell1

1 South Dakota State University, Brookings, SD, USA
2 Cornell University, Geneva, NY, USA

Contact the author*

Keywords

bud dormancy, freeze, chilling fulfilment

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Legacy of land-cover changes on soil erosion and microbiology in Burgundian vineyards

Soils in vineyards are recognized as complex agrosystems whose characteristics reflect complex interactions between natural factors (lithology, climate, slope, biodiversity) and human activities. To date, most of the unknown lies in an incomplete understanding of soil ecosystems, and specifically in the microbial biodiversity even though soil microbiota is involved in many key functions, such as nutrient cycling and carbon sequestration. Soil biological properties are indicative of soil quality. Therefore, understanding how soil communities are related to soil ecosystem functioning is becoming an essential issue for soil strategy conservation. Here, we propose to assess the importance of land-cover history on the present-day microbiological and physico-chemical properties. The studied area was selected in the Burgundian vineyards (Pernand-Vergelesses, Burgundy, France) where land occupation has been reconstructed over the last 40 years. Soil samples were collected in five areas reflecting various land cover history (forest, vineyards, shifting from forest to vineyards). For each area, physico-chemical parameters (pH, C, N, P, grain size) were measured and DNA was extracted to characterize the abundance and diversity of microbial communities. The obtained results show significant differences in the five areas suggesting that present-day microbial molecular biomass and bacterial taxonomic is partly inherited from past land occupation. Over longer period of time, such study of land-uses legacies may help to better assess ecosystem recovery and the impact of management practices for a better soil quality and vineyards sustainability.

Influence of light exclusion on anthocyanin composition in ‘Cabernet sauvignon’

The aim of this study was to determine how artificial shading influenced berry development and anthocyanin accumulation in ‘Cabernet sauvignon’. Opaque polypropylene boxes were applied to grape bunches over three different developmental stages.

Integrated approaches for the functional characterization of miRNAs in grapevine

Micro(mi)RNAs are small non-coding RNAs that regulate several pathways and are widely recognised as key players in plant development, tissue differentiation, and many other important physiological processes, including plant adaptation to biotic and abiotic stresses. The release of plant genomes and the application of high throughput sequencing have considerably extended miRNA discovery across many species, including grapevine (Vitis spp.). Despite their relevance in plant development, functional studies in grapevine to clarify the function of miRNAs are not yet available. Through the grapevine genetic improvement platform IMPROVIT at CNR-IPSP (http://www.ipsp.cnr.it/en/thematics/turin-headquarter-thematics/improvit/), we developed integrated approaches to discover miRNA function in grapevine.

“Silex vitioeno module porte-greffe”: an information system to gather experimental results on grapevine rootstocks

Maintaining stable yields and quality over time is a major challenge for the wine industry. Within the context of climate change, the choice of the rootstock is an important lever for adapting to current and future climatic conditions. Within a vineyard, the choice of the rootstock depends on the environmental conditions, the scion variety and the objectives of production. Many experimental data on the performances of rootstock already exist and can guide our decision-making.

Discrimination of white wines by Raman spectroscopy coupled with chemometric methods

France is the largest exporter of wine in the world. The export turnover is estimated at 8.7 billion euros in 2017 for 13 million hectoliters sold. This lucrative business pushes scammers to increase the value of some low-end wines by cheating on their appellations, quality or even their origins. These facts lead to losing 1.3 billion euros each year to the European Union’s wine and spirits companies.