terclim by ICS banner
IVES 9 IVES Conference Series 9 Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Abstract

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored. Four distinct conditions were analyzed 1) genotype difference with constant light/dark temperature conditions; 2) light vs dark with similar temperature conditions; 3) buds in light (14:00) at >0C vs <0C; 4) buds in dark (6:00 or 18:00) at >0C vs <0C; 4). Principal components analysis indicated that genotype accounted for 66% of variance and there were 1,916 and 1,559 differentially expressed genes (DEG) up and down regulated respectively, in Reisling relative to Cabernet Franc. A greater number of DEG were identified for light relative to dark samples (14:00 vs 6:00 or 18:00) and samples collected at temperatures >0C vs <0C. Gene pathway analysis showed significant positive enrichment in hormone signaling and secondary metabolite pathways in both genotypes in the >0C relative <0C temperature conditions indicating transient temperature changes enhance the metabolic activity of dormant buds.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Prakriti Sharma1, Jason P. Londo2, Anne Fennell1

1 South Dakota State University, Brookings, SD, USA
2 Cornell University, Geneva, NY, USA

Contact the author*

Keywords

bud dormancy, freeze, chilling fulfilment

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Canopy photosynthetic activity and water relations of Syrah/R99 as affected by row orientation on a particular terroir

L’activité photosynthétique et les relations hydriques de plantes de Syrah sur R99 un mois après la véraison ont été étudiées dans un vignoble de la région de Stellenbosch. Le vignoble, planté à 2,75 entre rangs et 1,5 m sur le rang, sur un sol de type Glenrosa, était en pente et exposé a l’ouest: pour les rangs on avait adopté une orientation nord

Arbuscular mycorrhizal fungi as biomarkers of vineyard yield in Champagne

The vine is colonized by a multitude of micro-organisms (fungi, bacteria, oomycetes) mainly coming from the microbial reservoir constituted by the soil. These microorganisms have positive or negative effects on the vine (protection against pathogens, resistance to abiotic stress, nutrition, but also triggering of diseases) (Fournier, Pellan et al. 2022). In addition to these functional roles, they respond quickly to environmental changes (climate, cultural practices) which could make them good bioindicators of the functioning of the wine ecosystem.

Multicriteria assessment of 11 agroecological viticulture systems during six years

Context and purpose of the study. Modern conventional agriculture, including viticulture, relies greatly on the use of chemical inputs, especially synthetic pesticides.

French wine sector facing climate change (part. 2) : the implementation of the national strategy

This summary follows this made by Hervé Hannin et al. Entitled “French wine sector facing climate change (part. 1) : a national strategy built on a foresight and participatory approach “. The french wine sector has taken a collective approach to the issue of climate change, and has officially submitted its strategy to the minister of agriculture in 2021. This industry policy is the result of multidisciplinary work carried out through the “laccave” project (metaprogramme accaf, inrae) and its prospective study designed to anticipate climate change in the french wine industry (aigrain p. Et al., 2016). French wine professionals decided to structure a strategy to deal with climate change du in particular to the presentation made at the 2016 OIV congress in Brazil.

Flor yeast diversity and dynamics in biologically aged wines

Wine biological aging is characterized by the development of yeast strains that form a biofilm on the wine surface after alcoholic fermentation. These yeasts, known as flor yeasts, form a velum that protects the wine from oxidation during aging. Thirty-nine velums aged from 1 to 6 years were sampled from “Vin jaune” from two different cellars. We show for the first time that these velums possess various aspects in term of color and surface aspects. Surprisingly, the heterogeneous velums are mostly composed of one species, S. cerevisiae. Scanning electron microscope observations of these velums revealed unprecedented biofilm structures and various yeast morphologies formed by the sole S. cerevisiae species.