terclim by ICS banner
IVES 9 IVES Conference Series 9 Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Abstract

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored. Four distinct conditions were analyzed 1) genotype difference with constant light/dark temperature conditions; 2) light vs dark with similar temperature conditions; 3) buds in light (14:00) at >0C vs <0C; 4) buds in dark (6:00 or 18:00) at >0C vs <0C; 4). Principal components analysis indicated that genotype accounted for 66% of variance and there were 1,916 and 1,559 differentially expressed genes (DEG) up and down regulated respectively, in Reisling relative to Cabernet Franc. A greater number of DEG were identified for light relative to dark samples (14:00 vs 6:00 or 18:00) and samples collected at temperatures >0C vs <0C. Gene pathway analysis showed significant positive enrichment in hormone signaling and secondary metabolite pathways in both genotypes in the >0C relative <0C temperature conditions indicating transient temperature changes enhance the metabolic activity of dormant buds.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Prakriti Sharma1, Jason P. Londo2, Anne Fennell1

1 South Dakota State University, Brookings, SD, USA
2 Cornell University, Geneva, NY, USA

Contact the author*

Keywords

bud dormancy, freeze, chilling fulfilment

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Understanding provenance and terroir in Australian Pinot noir

Aims: This study aimed to (1) characterise colour and phenolic profiles of commercial Australian Pinot noir wines, (2) understand regional drivers of sensory and volatile profiles of commercial Australian Pinot noir wines, and (3) generate a deeper understanding of where Australian Pinot noir wines profiles sit in an international context.

Deep learning based models for grapevine phenology

the phenological evolution is a crucial aspect of grapevine growth and development. Accurate detection of phenological stages can improve vineyard management, leading to better crop yield and quality traits. However, traditional methods of phenological tracking such as on-site observations are time-consuming and labour-intensive. This work proposes a scalable data-driven method to automatically detect key phenological stages of grapevines using satellite data. Our approach applies to vast areas because it solely relies on open and satellite data having global coverage without requiring any in-field data from weather stations or other sensors making the approach extensible to other areas.

Quali cantine perle strade del vino

Tutte le cantine possono aprirsi al pubblico? Evidentemente si, nessuno può impedire ad un produttore di accogliere i turisti.
Tutte le cantine possono far parte delle Strade del vino? No, perché la Strada del vino mette in gioco la reputazione della denominazione di origine alla quale è legata e le possibilità di sviluppo economico di un intero territorio.

Determination of steviol glycosides in wine by HPLC

The SCL laboratory in Bordeaux is one of the two official control laboratories for wine and wine products in france, under the authority of the ministry of finance and two of its general directorates: the DGCCRF (directorate general for competition, consumer affairs and fraud control) and the DGDDI (directorate general of customs and excise duties). In this capacity, it verifies the regulatory compliance of wines and investigates any possible falsifications or fraud. Steviol glycosides are natural sweeteners that are not authorized as additives in wine.

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha.