terclim by ICS banner
IVES 9 IVES Conference Series 9 Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Freeze-thaw temperature oscillations promote increased differential gene expression during grapevine bud dormancy

Abstract

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored. Four distinct conditions were analyzed 1) genotype difference with constant light/dark temperature conditions; 2) light vs dark with similar temperature conditions; 3) buds in light (14:00) at >0C vs <0C; 4) buds in dark (6:00 or 18:00) at >0C vs <0C; 4). Principal components analysis indicated that genotype accounted for 66% of variance and there were 1,916 and 1,559 differentially expressed genes (DEG) up and down regulated respectively, in Reisling relative to Cabernet Franc. A greater number of DEG were identified for light relative to dark samples (14:00 vs 6:00 or 18:00) and samples collected at temperatures >0C vs <0C. Gene pathway analysis showed significant positive enrichment in hormone signaling and secondary metabolite pathways in both genotypes in the >0C relative <0C temperature conditions indicating transient temperature changes enhance the metabolic activity of dormant buds.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Prakriti Sharma1, Jason P. Londo2, Anne Fennell1

1 South Dakota State University, Brookings, SD, USA
2 Cornell University, Geneva, NY, USA

Contact the author*

Keywords

bud dormancy, freeze, chilling fulfilment

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Measuring elemental sulfur in grape juice in relation to varietal thiol formation in Sauvignon blanc wines.

Aim: Sauvignon blanc displays a range of styles that can include prominent tropical and passionfruit aromas. Both sensory evaluation and chemical analysis have confirmed the above-average presence of ‘varietal thiols’ in the Sauvignon blanc wines from Marlborough, New Zealand.

Geographical indication “Brandy Italiano”: study on the influence of wood barrel toasting and natural seasoning on endogenous and wood-derived compounds of aged distillates

The European geographical indication (GI) Brandy Italiano is exclusively reserved to brandy obtained in Italy from the distillation of wine from grapes grown and vinified in the national territory [1].

Monitoring grapevine water status using Landsat 8 images: a two-year case study in a Merlot vineyard

Viticulture needs for spatial and temporal information are increasing to improve vineyard management, especially concerning water efficiency. Remote sensing, particularly from satellites, can be a powerful tool to assess vineyard characteristics such as vigor or water status in space-time. In this study, we use Landsat 8, an American Earth observation satellite with six bands from the visible (VIS) to the Short-Wave Infrared (SWIR) domains with 30m spatial resolution and two thermal bands with 100m spatial resolution.

Enhancing plant defense: carbon dots for efficient spray-induced gene silencing 

Ectopic RNA application for plant defense faces challenges in tree crops, including size, diffusion, and stability of active compounds such as ribonucleoproteins and nucleic acids. While existing strategies involve expressing dsRNA in transgenic plants targeting pathogens, our research strives to develop a transient RNAi system based on Spray-Induced Gene Silencing (SIGS). This approach aims to circumvent legal barriers and public concerns associated with genetically modified organisms (GMOs). Our strategy integrates SIGS with branched polyethyleneimine-functionalized Carbon Dots (bPEI-CDs) as nanocarriers, effectively addressing unique delivery challenges in plant defense as RNA stability and uptake enhancement

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.