terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the regulatory role of the grapevine MIXTA homologue in cuticle formation and abiotic stress resilience

Exploring the regulatory role of the grapevine MIXTA homologue in cuticle formation and abiotic stress resilience

Abstract

The outer waxy layer of plant aerial structures, known as the cuticle, represents an important trait that can be targeted to increase plant tolerance against abiotic stresses exacerbated by environmental transition. The MIXTA transcription factor, member of the R2R3-MYB family, is known to affect conical shape of petal epidermal cells in Anthirrinum, cuticular thickness in tomato fruit and trichome formation and morphology in several crops. The aim of this study was to investigate the role of the grapevine MIXTA homologue by phenotypic and molecular characterization of overexpressing and knock-out grapevine lines. The leaf cuticle was observed by light microscopy, indicating that stomatal density and other anatomical features, such as trichomes and pavement cell number, were affected by modulation of VviMIXTA. GC-MS analysis found that epicuticular wax loads and composition were similarly impacted. Physiological parameters collected on a randomized set of plants in controlled conditions showed that stomatal conductance was also affected. Selected lines, identified via VviMIXTA gene expression analysis, underwent RNA-seq to evaluate the transcriptomic impact of modulating VviMIXTA expression. The results were cross-referenced with DAP-seq data to identify MIXTA high confidence target genes. Additionally, further integration of the experimental data with in silico resources available for grapevine (e.g., OneGenE and aggregated tissue-specific GCNs) is being conducted for reconstructing MIXTA´s gene regulatory network. Our work explores the potential regulatory role of VviMIXTA in epidermal cell fate and cuticular wax composition in the grapevine leaf, paving the way for molecular breeding to enhance plant resilience and improve berry quality traits.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Carlotta Pirrello1*, Jenna Bryanne Jolliffe1,2, Lorenzo Vittani1, Luis Orduña3, Paolo Sonego1, Michele Faralli1,4, José Tomás Matus3, Stefania Pilati1, Justin Graham Lashbrooke2,5, Claudio Moser1

1 Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
2 South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
3 Institute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSIC, Paterna, 46908, Valencia, Spain
4 Center Agriculture Food Environment (C3A), University of Trento, via Mach 1, San Michele all’Adige, 38098, Italy
5 Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

Vitis vinifera, cuticle, stomata, trichomes, multi-omics data

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Culturable microbial communities associated with the grapevine soil in vineyards of La Rioja, Spain

The definition of soil health is complex due to the lack of agreement on adequate indicators and to the high variability of global soils. Nevertheless, it has been widely used as synonymous of soil quality for more than one decade, and there is a consensus warning of scientists that soil quality and biodiversity loss are occurring due to the traditional intensive agricultural practices.
In this work we monitored a set of soil parameters, both physicochemical and microbiological, in an experimental vineyard under three different management and land use systems: a) addition of external organic matter (EOM) to tilled soil; b) no tillage and plant cover between grapevine rows, and c) grapevines planted in rows running down the slope and tilled soil.

Accumulation of polyphenols in Barbera and Nebbiolo leaves during the vegetative season

Grapevine berries produce thousands of secondary metabolites of diverse chemical nature that have been largely detailed in the past due to their importance for defining wine quality. The wide Vitis vinifera diversity, resulting in thousands of different varieties well detailed in many studies regarding berries, is still not investigated in vegetative organs, leaves in particular. Deepening knowledge related to this aspect could be of great interest for many reasons (for example the possibility of using leaf extract for pharmaceutical, cosmetic and nutrition purposes) but, above all, for understanding the susceptibility of different grapevine varieties to pathogens.

MODULATION OF YEAST-DERIVED AROMA COMPOUNDS IN CHARDONNAY WINES USING ENCAPSULATED DIAMMONIUM PHOSPHATE TO CONTROL NUTRIENT RELEASE

Yeast-derived aroma compounds are the result of different and complex biochemical pathways that mainly occur during alcoholic fermentation. Many of them are related -but not limited- to the availability of nutrients in the fermentation medium and linked to nitrogen metabolism and biomass produced. Besides, the metabolic phase of yeast also regulates the expression of many enzymes involved in the formation of aroma active compounds. The work investigates the overall effect of continuous supplementation of nutrients during alcoholic fermentation of a grape must on the volatile composition of wines.

Application of satellite-derived vegetation indices for frost damage detection in grapevines

Wine grape production is increasingly vulnerable to freeze damage due to warming climates, milder winters, and unpredictable late spring frosts. Traditional methods for assessing frost damage in grapevines which combine fieldwork and meteorological data, are expensive, time-consuming, and labor-intensive. Remote sensing could offer a rapid, inexpensive way to detect frost damage at a regional scale. Remote sensing approaches were used to assess freeze damage in grapevines by evaluating satellite-derived vegetation indices (VIs) to understand the severity and spatial distribution of damage in several New York vineyards immediately after a frost event (May 17th-18th, 2023). PlanetScope 3m satellite images acquired before and after the freeze were used to map damage and measure changes in VIs for vineyards in the Finger Lakes region.

Bioprotective non-Saccharomyces yeast as an alternative to sulfites for the winemaking process

Sulfur dioxide (SO2) is used in winemaking due of its antioxidant, antioxydasic and antiseptic properties. Excessive amount of SO2 can negatively impact wine sensory perception and be detrimental for health. Agri-food industries are more transparent towards consumers concerning addition of sulfites, and oenology is no exception in this clairvoyance. As a consequence, the increase of consumers preference for wine with low or absent of sulfites addition is notorious. In this context, the impact of low/zero sulfites winemaking process on the microbial community should be evaluated. Moreover, microbial agents corresponding to bioprotective cultures represent a growing interest as an alternative to sulfites preservation in the early stages of vinification. However, scientific studies conducted to demonstrate their real effect are almost rare.