terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the regulatory role of the grapevine MIXTA homologue in cuticle formation and abiotic stress resilience

Exploring the regulatory role of the grapevine MIXTA homologue in cuticle formation and abiotic stress resilience

Abstract

The outer waxy layer of plant aerial structures, known as the cuticle, represents an important trait that can be targeted to increase plant tolerance against abiotic stresses exacerbated by environmental transition. The MIXTA transcription factor, member of the R2R3-MYB family, is known to affect conical shape of petal epidermal cells in Anthirrinum, cuticular thickness in tomato fruit and trichome formation and morphology in several crops. The aim of this study was to investigate the role of the grapevine MIXTA homologue by phenotypic and molecular characterization of overexpressing and knock-out grapevine lines. The leaf cuticle was observed by light microscopy, indicating that stomatal density and other anatomical features, such as trichomes and pavement cell number, were affected by modulation of VviMIXTA. GC-MS analysis found that epicuticular wax loads and composition were similarly impacted. Physiological parameters collected on a randomized set of plants in controlled conditions showed that stomatal conductance was also affected. Selected lines, identified via VviMIXTA gene expression analysis, underwent RNA-seq to evaluate the transcriptomic impact of modulating VviMIXTA expression. The results were cross-referenced with DAP-seq data to identify MIXTA high confidence target genes. Additionally, further integration of the experimental data with in silico resources available for grapevine (e.g., OneGenE and aggregated tissue-specific GCNs) is being conducted for reconstructing MIXTA´s gene regulatory network. Our work explores the potential regulatory role of VviMIXTA in epidermal cell fate and cuticular wax composition in the grapevine leaf, paving the way for molecular breeding to enhance plant resilience and improve berry quality traits.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Carlotta Pirrello1*, Jenna Bryanne Jolliffe1,2, Lorenzo Vittani1, Luis Orduña3, Paolo Sonego1, Michele Faralli1,4, José Tomás Matus3, Stefania Pilati1, Justin Graham Lashbrooke2,5, Claudio Moser1

1 Research and Innovation Centre, Edmund Mach Foundation, San Michele all’Adige, 38098, Italy
2 South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, 7600, South Africa
3 Institute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSIC, Paterna, 46908, Valencia, Spain
4 Center Agriculture Food Environment (C3A), University of Trento, via Mach 1, San Michele all’Adige, 38098, Italy
5 Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

Contact the author*

Keywords

Vitis vinifera, cuticle, stomata, trichomes, multi-omics data

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

VOLATILE, PHENOLIC AND COLORIMETRIC CHARACTERIZATION OF THREE DIFFERENT LAMBRUSCO APPELLATIONS

Lambrusco is a commercially successful sparkling red and rosé wine. With 13.06 million litres sold in 2021 was the second best-selling Italian wine after Chianti. According to National Catalogue of Vine Varieties there are thirteen Lambrusco Varieties with which to date are produced seven PDO wines. Among these, “Lambrusco Salamino di Santa Croce”, “Lambrusco Grasparossa di Castelvetro” and “Lambrusco di Sorbara” are the only ones that can be considered mono-varietal appellations, all located in Modena area. The PDOs contemplate the possibility of producing wines by secondary fermentation either in tank (Charmat method), or in bottle (Classico method). Sur lie is a third method commonly employed for Lambrusco, similar to the Classico method, from which differs for the absence of disgorgement.

Combined abiotic-biotic plant stresses on the roots of grapevine

In the 19th century, devastating outbreaks of phylloxera (Daktulosphaira vitifoliae Fitch), almost brought European viticulture to its knees. Phylloxera does not only take energy in form of sugars from the vine, but also affects the up- and down- regulations of genes, acts as a carbon sink and reprograms the physiology of the grapevines, including nutrient uptake and the defense system [1]. A key trait of rootstocks is the ability to perform well under high lime conditions as about 30 % of the land surface has calcareous soil. Iron deficiency not only causes the well-known problems of lime-induced chlorosis and stunted growth, but also affects the entire plant metabolism.

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.

Parcours de découverte des terroirs viticoles

A partir des recherches conduites sur la caractérisation des terroirs viticoles par des chercheurs de l’Unité de Recherches Vigne et Vin (1, 2, 3, 4, 5) du Centre INRA d’Angers, Terre des Sciences, le Centre de Culture Scientifique et Technique d’Angers (CCSTA) a mis au point un parcours de découverte d’une journée dans le vignoble angevin avec une approche pluridisciplinaire.

Biochemical responses of crimson seedless (Vitis vinifera) grapevines to altered micro climatic conditions and different water treatments in the Breede River Valley of South Africa

The South African Table grape industry has to expand to new markets with high quality niche products, but limited water availability threatens sustainable production. To overcome this challenge and to obtain high-quality products for the new markets, require constant technological advancement. Currently, limited available scientific information about growth balances and physiology and especially grape quality parameters, hinders technological advancement and thus efficient regulatory management of the morphological, chemical, and pathological status of table grapes, especially in response to abiotic factors.