terclim by ICS banner
IVES 9 IVES Conference Series 9 Heat-stress responses regulated via a MYB24-MYC2 complex

Heat-stress responses regulated via a MYB24-MYC2 complex

Abstract

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages. Half of URGs highly co-express with MYB24, and MapMan analysis discloses many significantly enriched heat-related terms. Specifically, 18 co-expressed URGs were reported as heat-induced genes. Due to the DNA-binding capacity of MYB24 and MYC2, we investigated their regulatory potential by taking advantage of DAP-seq data. More than 40 of these co-expressed URGs, named as MYB24/MYC2 high confidence targets (HCTs), are bound by both TFs or one of them in their 5kb upstream region. In particular, some HCTs have been previously and functionally validated as heat regulators or heat-induced genes. Furthermore, MYB24, MYC2, and a high proportion of their HCTs were significantly induced in reanalyzed heat-treatment transcriptomic studies. To sum up, our data suggests that the MYB24-MYC2 complex plays a key role in the hierarchical regulation of heat responses.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chen Zhang1, David Navarro-Paya1, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain

Contact the author*

Keywords

MYB24, MYC2, heat stress, transcriptomics, transcriptional regulation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Non-destructive his based analysis for shelf-life evaluation of table grape 

Fast, accurate, and non-destructive analytical techniques based on hyperspectral imaging (hsi) represent effective tools for food quality evaluation. A visible change in the appearance of a fresh product often negatively impacts the perceived quality from a consumer’s point of view.

Read More

Water recharge before budbreak and/or deficit irrigation during summer: agronomic effects on cv. Tempranillo in the D.O. Ribera del Duero

The availability of water in the soil and the water status of the vineyard are proving to be determining factors for crop management in the current context of climatic variation

Read More

VineAI: artificial intelligence for fungal disease

Early and accurate grapevine disease detection and surveillance are crucial for optimizing vineyard management practices.

Read More

Feasibility of pre-fermentative oenological tannins addition to enhance volatile composition and aroma perception in white wines

Oenological tannins (OETs) are an alternative to sulphur dioxide due to their antioxidant and antioxidase properties in the early phase of winemaking [1,2].

Read More

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Read More