terclim by ICS banner
IVES 9 IVES Conference Series 9 Heat-stress responses regulated via a MYB24-MYC2 complex

Heat-stress responses regulated via a MYB24-MYC2 complex

Abstract

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages. Half of URGs highly co-express with MYB24, and MapMan analysis discloses many significantly enriched heat-related terms. Specifically, 18 co-expressed URGs were reported as heat-induced genes. Due to the DNA-binding capacity of MYB24 and MYC2, we investigated their regulatory potential by taking advantage of DAP-seq data. More than 40 of these co-expressed URGs, named as MYB24/MYC2 high confidence targets (HCTs), are bound by both TFs or one of them in their 5kb upstream region. In particular, some HCTs have been previously and functionally validated as heat regulators or heat-induced genes. Furthermore, MYB24, MYC2, and a high proportion of their HCTs were significantly induced in reanalyzed heat-treatment transcriptomic studies. To sum up, our data suggests that the MYB24-MYC2 complex plays a key role in the hierarchical regulation of heat responses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chen Zhang1, David Navarro-Paya1, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain

Contact the author*

Keywords

MYB24, MYC2, heat stress, transcriptomics, transcriptional regulation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

INSIGHT THE IMPACT OF GRAPE PRESSING ON MUST COMPOSITION

The pre-fermentative steps play a relevant role for the characteristics of white wine [1]. In particular, the grape pressing can affect the chemical composition and sensory profile and its optimized management leads to the desired extraction of aromas and their precursors, and phenols resulting in a balanced wine [2-4]. These aspects are important especially for must addressed to the sparkling wine as appropriate extraction of phenols is expected being dependent to grape composition, as well.

Aging in amphorae with different porosity for sustainable production of Nero d’Avola wine

In recent years, the use of amphorae in winemaking has become more frequent, symbolizing a return to the origins of vinification to broaden the availability of wines with different style.

Comparative QTL mapping of phenology traits in three cross populations of grapevine

Long-term studies on grapevine phenology have clearly demonstrated that global warming is affecting phenological events, leading to an anticipation in their timing, and negatively impacting grape yield and berry quality. Therefore, dissecting the genetic determinants involved in the plant regulation of the phenological stages of budburst, flowering, veraison and ripening can improve our knowledge of the underlying mechanisms and support plant breeding programs and the advancement of vineyard management strategies.
We report here the results of a QTL mapping experiment conducted on three segregating populations obtained from the crossing of ‘Cabernet Sauvignon’ and ‘Corvina’, ‘Corvina’ and the hybrid ‘Solaris’ and ‘Rhine Riesling’ and ‘Cabernet Sauvignon’.

Effects of hormone- and natural-based elicitors at the transcriptomic level in berries of cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of the application of three hormone- and natural-based elicitors in Tempranillo.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.