terclim by ICS banner
IVES 9 IVES Conference Series 9 Heat-stress responses regulated via a MYB24-MYC2 complex

Heat-stress responses regulated via a MYB24-MYC2 complex

Abstract

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages. Half of URGs highly co-express with MYB24, and MapMan analysis discloses many significantly enriched heat-related terms. Specifically, 18 co-expressed URGs were reported as heat-induced genes. Due to the DNA-binding capacity of MYB24 and MYC2, we investigated their regulatory potential by taking advantage of DAP-seq data. More than 40 of these co-expressed URGs, named as MYB24/MYC2 high confidence targets (HCTs), are bound by both TFs or one of them in their 5kb upstream region. In particular, some HCTs have been previously and functionally validated as heat regulators or heat-induced genes. Furthermore, MYB24, MYC2, and a high proportion of their HCTs were significantly induced in reanalyzed heat-treatment transcriptomic studies. To sum up, our data suggests that the MYB24-MYC2 complex plays a key role in the hierarchical regulation of heat responses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chen Zhang1, David Navarro-Paya1, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain

Contact the author*

Keywords

MYB24, MYC2, heat stress, transcriptomics, transcriptional regulation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Diffuse light due to wildfire smoke enhances gas exchange of shaded leaves

The risk of wildfires is increasing as the frequency and severity of drought and heat waves continue to rise. Wildfires are associated with the combustion of plant materials and emit smoke. In the atmosphere, smoke may spread readily across large areas. Smoke is composed of solid and liquid phase particulates and gases and has been identified as a causal agent of “smoke taint” in wine. On a smoky day, the intensity of direct light decreases because these particulates scatter sunlight. Even though this effect is frequently assumed to decrease plant photosynthesis, this assumption ignores the potential changes in diffuse light and may be based on scant evidence.

Exploring relationships among grapevine chemical and physiological parameters and mycobiome composition under drought stress

Improving our knowledge on biotic and abiotic factors that influence the composition of the grapevine mycobiome is of great agricultural significance, due to potential effects on plant health, productivity, and wine characteristics. Among the various environmental factors affecting the morphological, physiological, biochemical and molecular attributes of grapevine, drought stress is one of the most severe, becoming increasingly an issue worldwide.

Effect of alcoholic strength on the phenolic and furfural compounds of Brandy de Jerez aged in Sherry Casks®

Brandy is a spirit drink produced from wine spirit aged for at least six months in oak casks with a capacity of less than 1000 L and minimum alcohol by volume (ABV) of 36%. During the aging process, physicochemical and sensory changes take place. Manifested by colour, flavour or aroma variations that improve the quality of the initial distillate.

Cold plasma at atmospheric pressure for eliminating Brettanomyces from oak wood

In the oenological industry, the maintenance and sanitation of oak barrels has become a fundamental task. The wood has a porous structure that facilitates the penetration not only of the wine, but of the microorganisms it contains, such as the alterative yeast Brettanomyces bruxellensis.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.