terclim by ICS banner
IVES 9 IVES Conference Series 9 Heat-stress responses regulated via a MYB24-MYC2 complex

Heat-stress responses regulated via a MYB24-MYC2 complex

Abstract

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages. Half of URGs highly co-express with MYB24, and MapMan analysis discloses many significantly enriched heat-related terms. Specifically, 18 co-expressed URGs were reported as heat-induced genes. Due to the DNA-binding capacity of MYB24 and MYC2, we investigated their regulatory potential by taking advantage of DAP-seq data. More than 40 of these co-expressed URGs, named as MYB24/MYC2 high confidence targets (HCTs), are bound by both TFs or one of them in their 5kb upstream region. In particular, some HCTs have been previously and functionally validated as heat regulators or heat-induced genes. Furthermore, MYB24, MYC2, and a high proportion of their HCTs were significantly induced in reanalyzed heat-treatment transcriptomic studies. To sum up, our data suggests that the MYB24-MYC2 complex plays a key role in the hierarchical regulation of heat responses.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chen Zhang1, David Navarro-Paya1, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain

Contact the author*

Keywords

MYB24, MYC2, heat stress, transcriptomics, transcriptional regulation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Grapevine root system architecture: empirical insights and first steps towards in silico studies

Root System Architecture (RSA) is crucial for plant resilience and resource uptake, yet remains underexplored in viticulture.

Read More

Reduction of the height of the canopy in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

Global warming is accelerating the technological ripening of the grape, with a loss of acidity, which requires that vineyard management can delay ripening to avoid it. The source-sink relation is essential for grape ripening, since it affects the distribution of photosynthates and substances derived from plant metabolism. A work is proposed to know the response of the vineyard to the drastic reduction of the foliar surface by trim down the shoots in cv.

Read More

Diversity and internationalization of wine grape varieties: Evidence from a revised global database

Aim: To quantify the extent to which national mixes of wine grape varieties (in terms of vineyard bearing area) have become more or less diversified, and ‘internationalized’, since wine globalization accelerated from the 1990s.

Read More

The sensitivity to ABA affects the cross-talk between scion/rootstock in tolerant grapevines to drought stress

Drought caused by climate change has a dramatic incidence on the vineyard. Despite employing specific rootstocks tolerant to drought like 110 Richter, the vineyard continues to experience various losses, revealing the importance of the scion cultivar in the adaptation to drought stress. In this regard, Merlot, a widely cultivated grapevine, exhibited reduced drought tolerance compared to less cultivated varieties like Callet, a local cultivar originating from the Balearic Islands that demonstrated greater resilience to drought. Therefore, understanding the drought stress response in both cultivars and the cross-talk between scion and rootstock is key to unveiling possible differences that could affect to the adaptation to drought in vineyard.

Read More

POTENTIAL DEACIDIFYING ROLE OF A COMMERCIAL CHITOSAN: IMPACT ON PH, TITRATABLE ACIDITY, AND ORGANIC ACIDS IN MODEL SOLUTIONS AND WHITE WINE

Chitin is the main structural component of a large number of organisms (i.e., mollusks, insects, crustaceans, fungi, algae), and marine invertebrates including crabs and shrimps. The main derivative of chitin is chitosan (CH), produced by N-deacetylation of chitin in alkaline solutions. Over the past decade, the OIV/OENO 338A/ 2009 resolution approved the addition of allergen-free fungoid CH to must and wine as an adjuvant for microbiological control, prevention of haziness, metals chelation and ochratoxins removal (European Commission. 2011). Despite several studies on application of CH in winemaking, there are still very limited and controversial data on its interaction with acidic components in wine (Colan-gelo et al., 2018; Castro Marin et al., 2021).

Read More