terclim by ICS banner
IVES 9 IVES Conference Series 9 Understanding the expression of gene families involved in anthocyanin biosynthesis during berry ripening: Tannat as a case study

Understanding the expression of gene families involved in anthocyanin biosynthesis during berry ripening: Tannat as a case study

Abstract

The quality of wine is assessed, among other things, by its color, which is mainly due to its anthocyanin content. These pigments are polyphenols that give red, purple and blue hues depending on the relative proportion of anthocyanins produced by the action of flavonoid 3’5′ hydroxylase (delphinidin-3-glucoside, petunidin-3-glucoside, malvidin-3-glucoside) or flavonoid 3′ hydroxylase (cyanidin-3-glucoside, peonidin-3-glucoside). To study the genes involved in this biosynthetic pathway, we focused on Vitis vinifera cv. Tannat, known for producing wines with higher anthocyanin content and darker purple color compared to most red grape varieties. In this work, we have performed RNA-Seq analysis of skins during berry development, taking green and red berries at 50% veraison as separate samples, as an experimental strategy to focus on the differential expression of genes of interest. Several novel (not previously annotated in the Pinot noir reference genome PN40024 12X) and varietal (not in the reference genome) genes showed differential expression associated with color change during veraison. Expression of a novel F3’5’H gene and a varietal F3’H gene was confirmed by RT-qPCR in the same samples used for RNA-Seq and in another growing season. This work establishes the specific expression fingerprint for gene families (CHS, CHI, F3H, F3’H, F3’5’H, DFR, LDOX, 3GT, OMT, MYBA1, MYBA2, MYBA3) directly involved in anthocyanin biosynthesis and regulation during Tannat veraison.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Cecilia C. Da Silva1*, Eduardo Boido2, Carina Gaggero3, Massimo Delledonne4, Francisco Carrau2

1 PDU Espacio de Biología Vegetal del Noreste, sede Tacuarembó, CENUR Noreste, Universidad de la República, Tacuarembó, Uruguay
2 Área Enología y Biotecnología de Fermentaciones, Facultad de Química, Universidad de la República, Montevideo, Uruguay
3 Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
4 Centro di Genomica Funzionale, Dipartimento di Biotecnologie, Universitá degli Studi di Verona, Verona, Italy

Contact the author*

Keywords

Anthocyanins, Vitis vinifera, Tannat, RNA-Seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: EVOLUTION IN BOTTLED WINE

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors. The impact on wine aroma is mainly attributed to volatile phenols, while in-mouth hydrolysis of glycosylated forms may be responsible for long-lasting “ashy” aftertastes (1).

Characterization and biological effects of extracts from winery by-products

Pomace, stem, grapevine leaves, and vine shoots arise as so called winery by-products during the wine production process.

Screening of different commercial wine yeast strains: the effect of sugar and copper additions on fermentation and volatile acidity production

The aims of this study were to examine the effect of high sugar concentrations of must and copper residues on different commercial wine yeasts. Copper originating from pesticides has been known to inhibit yeast, but it’s effect on fermentation performance and VA production of different yeast strains had not been investigated in detail.

Somatic embryogenesis and organogenesis: driving regeneration forces behind grapevine genetic transformation

Cell pluripotency, enables the possibility to change the cellular fate, stimulating the reorganization and the formation of new vegetative structures from differentiated somatic tissues. Although several factors are implicated in determining the success of a breeding program through the use of modern biotechnological techniques, the definition of a specific regeneration strategy is fundamental to speed up and make these applications feasible.

Within-vineyard variability in grape composition at the estate scale can be assessed through machine-learning modeling of plant water status in space and time. A case study from the hills of Adelaida District AVA, Paso Robles, CA, USA

Aim: Through machine-learning modelling of plant water status from environmental characteristics, this work aims to develop a model able to predict grape phenolic composition in space and time to guide selective harvest decisions at the estate scale.