terclim by ICS banner
IVES 9 IVES Conference Series 9 Regulation of terpene production in methyl jasmonate treated cell-cultures

Regulation of terpene production in methyl jasmonate treated cell-cultures

Abstract

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.  MapMan enrichment analysis on their TOP420 co-expressed genes (CEGs) allowed us to delimit some TFs highly enriched in jasmonate-related terms. This was the case of VviMYC2, the only grape member of the bHLH IIIe subgroup, and the best candidate for studying the regulation of jasmonate signaling. We confirmed the binding potential of MYC2 by DAP-seq, and combining it to the list of MeJA-URGs and MYC2-CEGs, we generated a list of high-confidence targets that included jasmonate-related genes and TFs such as MYB24, previously found to interact with MYC2 and required for the activation of terpenoid genes. In concordance, our MeJA data showed 13 significantly induced TPS genes, 9 of which are bound by MYB24, MYC2 or both. A few terpenoid compounds associated with the induced TPSs were significantly accumulated by MeJA. Our data suggests MYC2 regulates the jasmonate pathway and mediates terpene production cooperating with MYB24 in response to MeJA.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jone Echeverria1, Chen Zhang1, Chiara Foresti2 Antonio Santiago1, Luis Orduña1, Paolo Sonego3, Massimo Pindo3, Sara Zenoni2, Marco Moretto3, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
2 Department of Biotechnology, University of Verona, 37134, Verona, Italy
3 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy

Contact the author*

Keywords

gene expression, plant cell suspensions, terpenes, methyl jasmonate, transcription factors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Politics meets terroir. The story of Prosecco – Are GI’s just a protectionist racket?

The recent Free Trade Agreement negotiations between Australia and the European Union have again put the issue of Geographical Indications (GIs) in the spotlight. Australia has long demonstrated its understanding of GIs and maintains a clear and rigorous GI protection system for wine. For many years, Australia’s wine sector was a strong advocate for GIs and a strong system to protect the

Read More

VitiCanopy to measure vine pruning mass and assess above ground vineyard carbon storage

The wine industry is increasingly focused on carbon accounting due to consumer’s demand and the industry’s goal of reducing carbon emissions.

Read More

A browser application for comprehensive 3-dimensional LC × LC × IM – MS data analysis to study grape and wine polyphenols

The analysis of structurally diverse proanthocyanidins in grapes and wine is challenging. Comprehensive two-dimensional liquid chromatography (LC×LC) and ion mobility spectrometry-mass spectrometry (IMS-MS) are increasingly used to address the challenges associated with the analysis of highly complex samples such as wine and grapes

Read More

Different yield regulation strategies in semi-minimal-pruned hedge (SMPH) and impact on bunch architecture

Yields in the novel viticulture training system Semi-Minimal-Pruned Hedge (SMPH) are generally higher compared to the traditional Vertical Shoot Positioning (VSP). Excessive yields have a negative impact on the vine and wine quality, which can result in substantial losses in yield in subsequent vintages (alternate bearing) or penalties in fruit quality. Therefore yield regulation is essential. The bunch architecture in SMPH differs from VSP. Generally there is a higher amount but smaller bunches with lower single berry weights in SMPH compared to VSP.

Read More

Using remotely sensed (UAV) and in situ field measurements to describe grapevine canopy characteristics

Row orientation and canopy management are essential for high quality grapevine production. Microclimatic conditions of the leaves and fruits can be influenced by the canopy geometry. Remote sensing is a very promising tool to describe vegetative growth and physiological behavior of vineyards. However, the correlation between remotely sensed data and in situ field measurements has been described scarcely in the scientific literature so far. The aim of the study was to correlate remotely sensed data obtained with Unmanned Aerial Vehicle (UAV) with in situ field measurements to describe canopy structure.

Read More