terclim by ICS banner
IVES 9 IVES Conference Series 9 Regulation of terpene production in methyl jasmonate treated cell-cultures

Regulation of terpene production in methyl jasmonate treated cell-cultures

Abstract

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.  MapMan enrichment analysis on their TOP420 co-expressed genes (CEGs) allowed us to delimit some TFs highly enriched in jasmonate-related terms. This was the case of VviMYC2, the only grape member of the bHLH IIIe subgroup, and the best candidate for studying the regulation of jasmonate signaling. We confirmed the binding potential of MYC2 by DAP-seq, and combining it to the list of MeJA-URGs and MYC2-CEGs, we generated a list of high-confidence targets that included jasmonate-related genes and TFs such as MYB24, previously found to interact with MYC2 and required for the activation of terpenoid genes. In concordance, our MeJA data showed 13 significantly induced TPS genes, 9 of which are bound by MYB24, MYC2 or both. A few terpenoid compounds associated with the induced TPSs were significantly accumulated by MeJA. Our data suggests MYC2 regulates the jasmonate pathway and mediates terpene production cooperating with MYB24 in response to MeJA.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jone Echeverria1, Chen Zhang1, Chiara Foresti2 Antonio Santiago1, Luis Orduña1, Paolo Sonego3, Massimo Pindo3, Sara Zenoni2, Marco Moretto3, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
2 Department of Biotechnology, University of Verona, 37134, Verona, Italy
3 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy

Contact the author*

Keywords

gene expression, plant cell suspensions, terpenes, methyl jasmonate, transcription factors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Overall conceptual characterization of aged dry white wines using a mental descriptive questionnaire

The purpose of the present study was to understand the overall concept of an aged dry white wine using a descriptive mental questionnaire. A total of 680 worldwide participants, grouped according to their involvement in the wine business, replied to an online questionnaire to characterize the sensory analytical and synthetic descriptors of an aged dry white wine. The descriptors were selected using a Check-All-That-Apply (CATA) approach concerning wine colour, aroma, taste, mouthfeel, and global appreciation.

The influence of culture medium on the dynamics of fermentation of wine yeasts

Wine yeast strains Saccharomyces ellipsoideus have important applications in food industry and in this regard is sought isolation as pure cultures and selecting those strains, which in laboratory investigations which have great biotechnological properties This study was intended as the ratio of live cells and autolysates cells also the influence of culture medium on this report. Yeasts selected for this study were isolated from industrial strains of indigenous grape varieties, namely: Feteasca Royal (FR) Feteasca White (FA), black Feteasca (FN), Romanian Tamaioasa (TR), Babeasca Black (BN) and Cotnari Grasa (GC).

Distribution and sensory impact of new oak wood-derived compounds in wines

Despite the numerous research studies carried out in recent years, the study of wine aroma remains of great interest due to its complexity. Wine maturation in oak barrels is described as an important step in the production of quality wines. In fact, oak wood develops several aromatic nuances through its toasting which can be released into the wine. A great deal of work has been performed in order to identify the wood-derived volatile compounds that contribute to wine aroma (e.g., whisky-lactone, maltol, eugenol, guaiacol, vanillin).

Plastic debris at vines: carriers of pollutants in the environment?

Modern agriculture employs large amounts of plastics, such as mulching and greenhouse films, thermal covers, plant protection tubes and tying tape. The latter two types are very common in viticulture. Guard tubes are employed to protect young vines from mechanic and atmospheric damage, whilst polymeric tying tape has replaced natural-origin materials to hold the canopy of vines. Both materials are made on synthetic polymers, which include a range of additives to improve their environmental stability remaining in the environment of vineyards for years. During this time, they are exposed to the range of pesticides (fungicides, insecticides and in a lesser extend herbicides) applied to vines.

Generation of functional chitosan derivatives to better understanding the antiseptic effect on Brettanomyces bruxellensis in wine

The addition of fungal chitosan in wine is allowed since 2009 to release some spoilage microorganisms such as Brettanomyces bruxellensis (OIV/OENO 338A/2009; EC 53/2011). This yeast is able to produce volatil phenols and is responsible of organoleptic deviations compromising quality and typicality of red wines [1]. Despite the fact that fungal chitosan is highly renewable, no toxic and non-allergenic, its use remains marginal because this treatment is relatively recent (compare to sulphites treatment) and information are contradictory between different studies described in literature. For all these reasons,