Regulation of terpene production in methyl jasmonate treated cell-cultures

Grapevine is one of the most-frequently phytosanitary treated crop systems. Consequently, restrictions have been applied by the European Commission on the number of pesticide treatments and the maximum quantity of copper fungicides allowed per year. Moreover, there is a need and an increasing demand for more ecological-sustainable agricultural products.
Seaweeds are currently used as fertilizers in viticulture, as they have been proven to be beneficial in several ways related to growth and nutrition.

The ripening of grapevine berries encompasses complex morphological and physiological processes, especially at veraison. Berry shrivel (BS) is a ripening physiological disorder affecting grape berries with visible symptoms appearing short after veraison. The main symptoms of BS are a strong reduction in sugar accumulation, inhibited anthocyanin biosynthesis and high pH values. The most popular red grape cultivar in Austria “Blauer Zweigelt” (Vitis vinifera L.) is specifically prone to develop the BS ripening disorder and up to date a no specific cause or causes could be identified. Recently omics approaches have identified and characterized key processes during grapevine ripening. Among them a small subset of genes, called SWITCH, have been described as markers for the onset of the ripening process in fruits.

Reduction of sulfur dioxide during winemaking is a request from the wine industry. To replace sulfur dioxide, various alternatives exist, including bioprotection by yeast inoculation. This practice consists in adding non-Saccharomyces yeasts directly on the grapes or must.

Climate changes and the trend towards organic and more sustainable winemaking highlighted the need to use biological methodologies. The reduction in the use of SO2, the need of the reduction of ethanol content of wines and the now need to reduce or eliminate chemical phytosanitary products, have prompted the search for alternative practices.

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.