terclim by ICS banner
IVES 9 IVES Conference Series 9 Regulation of terpene production in methyl jasmonate treated cell-cultures

Regulation of terpene production in methyl jasmonate treated cell-cultures

Abstract

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.  MapMan enrichment analysis on their TOP420 co-expressed genes (CEGs) allowed us to delimit some TFs highly enriched in jasmonate-related terms. This was the case of VviMYC2, the only grape member of the bHLH IIIe subgroup, and the best candidate for studying the regulation of jasmonate signaling. We confirmed the binding potential of MYC2 by DAP-seq, and combining it to the list of MeJA-URGs and MYC2-CEGs, we generated a list of high-confidence targets that included jasmonate-related genes and TFs such as MYB24, previously found to interact with MYC2 and required for the activation of terpenoid genes. In concordance, our MeJA data showed 13 significantly induced TPS genes, 9 of which are bound by MYB24, MYC2 or both. A few terpenoid compounds associated with the induced TPSs were significantly accumulated by MeJA. Our data suggests MYC2 regulates the jasmonate pathway and mediates terpene production cooperating with MYB24 in response to MeJA.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jone Echeverria1, Chen Zhang1, Chiara Foresti2 Antonio Santiago1, Luis Orduña1, Paolo Sonego3, Massimo Pindo3, Sara Zenoni2, Marco Moretto3, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
2 Department of Biotechnology, University of Verona, 37134, Verona, Italy
3 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy

Contact the author*

Keywords

gene expression, plant cell suspensions, terpenes, methyl jasmonate, transcription factors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

EVIDENCE OF THE INTERACTION OF ULTRASOUND AND ASPERGILLOPEPSINS I ON UNSTABLE GRAPE PROTEINS

Most of the effects of ultrasound (US) result from the collapse of bubbles due to cavitation. The shockwave produced is associated with shear forces, along with high localised temperatures and pressures. However, the high-speed stream, radical species formation, and heat generated during sonication may also affect the stability of some enzymes and proteins, depending on their chemical structure. Recently, Ce-lotti et al. (2021) reported the effects of US on protein stability in wines. To investigate this further, the effect of temperature (40°C and 70°C; 60s), sonication (20 kHz and 100 % amplitude, for 20s and 60s, leading to the same temperatures as above, respectively), in combination with Aspergillopepsins I (AP-I) supplementation (100 μg/L), was studied on unstable protein concentration (TLPs and chitinases) using HPLC with an UV–Vis detector in a TLPs-supplemented model system and in an unstable white wine.

Importance of the Terror Variability Map (TVM) in Precision viticulture (PV): choice of methodology for soil classification

The Precision Viticulture (PV) is defined “as a management system that is information and technology based, is site specific and uses one or more of the following sources of data: soils, vigour, nutrients, pests, moisture, and yield among others, for optimum profitability, sustainability, and protection of the environment” (OIV, 2018, in process). The elements mentioned in the definition are an important part of the terroir components. The terroir is a tool In Viticulture, it is the analysis and study unit, and the variability of a certain situation can be due to any difference in every element or property of each factor that constitutes it, including the management.The soil and its management are those that bring the most variability to terroir.

Effects of water and nitrogen uptake, and soil temperature, on vine development, berry ripening and wine quality of Cabernet-Sauvignon, Cabernet franc and Merlot (Saint-Emilion, 1997)

Wine quality depends largely on berry ripening conditions in relation to soil and climat. The influence of the soil has been studied in Bordeaux since the early Seventies (SEGUIN, 1970; DUTEAU et al., 1981; VAN LEEUWEN, 1991; VAN LEEUWEN et SEGUIN, 1994) and, more recently, in the Val de Loire (MORLAT, 1989), the Alsace (LEBON, 1993) and the Costières de Nîmes regions (MARTIN, 1995).

Adaptation of Lactobacilli towards low ph and SO2 to develop MLF in base musts for sparkling wines

In some white wines, malolactic fermentation (MLF) is very interesting, and for low pH wines this process is particularly difficult. Although MLF is generally not recommended for sparkling white wine, some winemakers prefer to promote MLF to contribute to organoleptic complexity. Oenococcus oeni is generally the bacterium of choice for MLF.

Using elicitors in different grape varieties. Effect over their phenolic composition

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known.