terclim by ICS banner
IVES 9 IVES Conference Series 9 Regulation of terpene production in methyl jasmonate treated cell-cultures

Regulation of terpene production in methyl jasmonate treated cell-cultures

Abstract

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.  MapMan enrichment analysis on their TOP420 co-expressed genes (CEGs) allowed us to delimit some TFs highly enriched in jasmonate-related terms. This was the case of VviMYC2, the only grape member of the bHLH IIIe subgroup, and the best candidate for studying the regulation of jasmonate signaling. We confirmed the binding potential of MYC2 by DAP-seq, and combining it to the list of MeJA-URGs and MYC2-CEGs, we generated a list of high-confidence targets that included jasmonate-related genes and TFs such as MYB24, previously found to interact with MYC2 and required for the activation of terpenoid genes. In concordance, our MeJA data showed 13 significantly induced TPS genes, 9 of which are bound by MYB24, MYC2 or both. A few terpenoid compounds associated with the induced TPSs were significantly accumulated by MeJA. Our data suggests MYC2 regulates the jasmonate pathway and mediates terpene production cooperating with MYB24 in response to MeJA.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Jone Echeverria1, Chen Zhang1, Chiara Foresti2 Antonio Santiago1, Luis Orduña1, Paolo Sonego3, Massimo Pindo3, Sara Zenoni2, Marco Moretto3, José Tomás Matus1*

1 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
2 Department of Biotechnology, University of Verona, 37134, Verona, Italy
3 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy

Contact the author*

Keywords

gene expression, plant cell suspensions, terpenes, methyl jasmonate, transcription factors

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Heatwaves impacts on grapevine physiology, berry chemistry & wine quality

Climate change impacts on both yields and quality have increased over the past decades, with the effects of extreme climate events having the most dramatic and obvious impacts. Increasing length and intensity of heatwaves associated with increased water stress necessitates a reevaluation of climate change responses of grapevine and, ultimately, a reconsideration of vineyard management practices under future conditions. Here we summarize results from a three-year field trial manipulating irrigation prior to and during heatwave events to assess impacts of water application rates on vine health and physiology, berry chemistry, and wine quality. We also highlight potential mitigation strategies for extreme heat, both in terms of water application, as well as other cultural practices that could be widely applicable.

Shoot positioning: effect on physiological, vegetative and reproductive parameters

[English version below]

On a étudié durant deux saisons de croissance (2002/2003 et 2003/2004) l’effet de l’orientation vertical des rameaux sur les paramètres physiologiques, végétatifs et reproductifs dans la région de Stellenbosch dans un vignoble du cépage Merlot sur 99 R conduite à espalier et taillé a cordon coursonné. Les vignes étaient espacées 2.7 x 1.5 m. L’irrigation a été appliquée quand la baie avait la dimension d’un pois et a la véraison.

Water and physiological response to early leaf removal of cv. Verdejo in rainfed conditions, at different times of the day, in the D.O. Rueda (Spain)

Aim: Early leaf removal, generally applied before flowering, is mostly conceived as a technique to control grape yield and improve the health of grapes and focused on the final objective of increasing wine quality.

Enhancing sustainability in viticulture through digital technologies: A case study from Smyrnakis winery

The integration of digital technologies in vineyard management offers substantial opportunities for enhancing sustainability, efficiency, and grape quality.

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.