terclim by ICS banner
IVES 9 IVES Conference Series 9 Vineyard microclimate alterations induced by black mulch through transcriptome reshaped the flavoromics of Cabernet Sauvignon

Vineyard microclimate alterations induced by black mulch through transcriptome reshaped the flavoromics of Cabernet Sauvignon

Abstract

To alter the vineyard microclimate and produce quality wine under a semi-arid climate, black geotextile inter-row mulch (M) was applied for two vintages (2016-2017). The grapes were sampled at three growing stages to conduct the untargeted metabolome and transcriptome analysis. The upregulated genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered severe heat stress, resulting in lower sugar and higher acids at harvest. The integration of metabolome and transcriptome analysis identified the key genes responsible for the enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes and norisoprenoids concentrations in M grapes. The effects of metabolites and transcriptome were more evident in vintage with weaker light indicating the key regulator of light exposure under semi-arid regions. In addition, the potential key transcription factors regulating the biosynthesis of the above metabolites including VviGATA11, VviHSFA6B, and VviWRKY03 were identified through weighted correlation network analysis. Taken together, this study provides a valuable overview of metabolic and transcriptomic responses of grapes exposed to inter-row mulch treatment in semi-arid climates, which could facilitate understanding the complex regulatory network of metabolites in response to microclimate changes.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Meng-Bo Tian1,2, Yu Wang1,2, Jun Wang1,2,*

1 Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2 Key Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing 100083, China

Contact the author*

Keywords

grape, floor management, microclimate, phenolic compounds, volatile compounds

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Ecophysiological characterisation of terroir effects on Vitis vinifera L. Chardonnay and pinot noir in south african cool climate regions

Terroir encompasses environmental (climate, geology, soil and topography), genetic (cultivar and clone) and human factors (oenological and viticultural practices). Climate change brings about shifts in the suitability of a region for the growth of specific grapevine cultivars. This study focused on climatic and fruit parameters (berry size, weight, pH, total acidity (TA) and phenolics) to characterise the terroir effect in Vitis vinifera L. cultivars Chardonnay and Pinot Noir vineyards in the Cape South Coast region (Walker Bay and Elgin).

DNA-free editing to improve stress resilience of wine grape genotypes recalcitrant-to-regeneration

Wine viticulture, being firmly linked to the vine-terroir relationship, has always encountered significant bottlenecks to genetic innovation. Nonetheless, the development of new breeding strategies leading to the selection of stress resilient genotypes is urgent, especially in viticulture, where it would allow reducing the use of chemical treatments adopted to control fungal diseases. Genome editing represents an extremely promising breeding technique. Unfortunately, the well-known recalcitrance of several wine grape cultivars to in vitro regeneration strongly limits the exploitation of this approach, which to our knowledge has so far been developed on table grape genotypes with high regeneration potential.

Evaluation of two transmittance meters in estimating chlorophyll and nitrogen concentrations in grapevine cultivars

Two transmittance-based chlorophyll meters (SPAD-502 and CCM-200) were evaluated in estimating chlorophyll (Chl) and nitrogen (N) levels in grapevine leaves.

How sensory quality of wines can be accessed as a trait in MAS grape vine breeding

In the context of the global crises of global warming, biodiversity and pollution, current agricultural practices need to be reconsidered.

Unraveling the complexity of high-temperature tolerance by characterizing key players of heat stress response in grapevine

Grapevine (Vitis spp.) is greatly influenced by climatic conditions and its economic value is therefore directly linked to environmental factors. Among these factors, temperature plays a critical role in vine phenology and fruit composition. In such conditions, elucidating the mechanisms employed by the vine to cope with heat waves becomes urgent. For the past few years, our research team has been producing molecular and metabolic data to highlight the molecular players involved in the response of the vine and the fruit to high temperatures [1]. Some of these temperature-sensitive genes are currently undergoing characterization using transgenesis approaches coupled or not with genome editing, taking advantage of the Microvine genotype [2].