terclim by ICS banner
IVES 9 IVES Conference Series 9 Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Abstract

Viticulture is facing many challenges due to climate change effects with increasingly attention to save resources, such as water, considering that drought events have been predicted to dramatically increase over the next future. Thanks to the -omics techniques, research pushed forward knowledge to deepen facets of drought response in diverse grapevine-rootstock combinations. However, the regulatory mechanisms orchestrating adaptation strategies during drought and recovery in grafted grapevines need further exploration. Herein, we combined ecophysiological, biochemical and molecular approaches to unravel drought and recovery-induced changes in potted Nebbiolo (NE) plants grafted onto three different rootstocks (3309, Kober5BB, Gravesac), by analysing root and leaf tissues. Rhizosphere bacterial dynamics and arbuscular mycorrhizal fungi (AMF) colonization have also been investigated during drought and recovery phases. Leaf ecophysiology and water relations were monitored over time, revealing diverse behaviours at severe stress (SS) and recovery (REC) conditions. Overall, combining anatomical, biochemical and gene expression data of stress-associated markers involved in ABA metabolism, osmolytes, antioxidant pathways and xylem features from both bionts, different ABA- or osmotic-dependent responses were observed at SS and REC for NE/3009 and NE/Gravesac combinations. Conversely, the NE/Kober5BB combination appeared to be in a primingstate, displaying higher root ABA content and AM colonization prior to stress imposition, along with a lower scion xylem area. These findings underscore the varied strategies employed by different grafted combinations in drought recovery, considering grapevines not as standalone entities, but rather as holobionts (with their inhabiting microbes) interacting collectively with the surrounding environment.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1,2, Nicola Belfiore1, Amedeo Moine2, Chiara Pagliarani2, Cristina Morabito3, Francesca Secchi3, Loredana Moffa1, Marco Sandrini1, Raffaella Balestrini2, Irene Perrone2, Giorgio Gambino2, Federica Gaiotti1, Danila Cuozzo2,3, Ivana Gribaudo2, Franco Mannini2, Claudio Lovisolo3 and Walter Chitarra1,2*

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

Rootstock, metabarcoding, gas exchange, AMF, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensory impact of sunburn in white wine and mitigation of climateinduced off-flavours by defoliation and application of reflecting particles on grapes

Climate change is a great environmental challenge with large impact on the Wine and sprakling wine industry. Heat waves and dryness cause frequent sunburn damage in white grapes

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

 The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies.

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

Geospatial technologies in spatially defined viticulture: case study of a vineyard with Agiorgitiko variety in Koutsi, Nemea, Greece

Geospatial technologies have significant contribution to viticulture, especially in small-scale vineyards, which require precise management. Geospatial data collected by modern technologies, such as Unmanned Aerial Vehicle (UAV) and satellite imagery, can be processed by modern software and easily be stored and transferred to GIS environments, highlighting important information about the health of vine plants, the yield of grapes and the wine, especially in wine-making varieties. The identification of field variability is very important, particularly for the production of high quality wine. Modern geospatial data management technologies are used to achieve an easy and effortless localization of the fields’ variability.

From precursor identification to the study of the distribution of 3-methyl-2,4-nonanedione in red wines and spirits

Prematurely aged red wines are marked by intense prune and fig aromatic nuances that dominate the complex bouquet that can be achieved through bottle aging.