terclim by ICS banner
IVES 9 IVES Conference Series 9 Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Abstract

Viticulture is facing many challenges due to climate change effects with increasingly attention to save resources, such as water, considering that drought events have been predicted to dramatically increase over the next future. Thanks to the -omics techniques, research pushed forward knowledge to deepen facets of drought response in diverse grapevine-rootstock combinations. However, the regulatory mechanisms orchestrating adaptation strategies during drought and recovery in grafted grapevines need further exploration. Herein, we combined ecophysiological, biochemical and molecular approaches to unravel drought and recovery-induced changes in potted Nebbiolo (NE) plants grafted onto three different rootstocks (3309, Kober5BB, Gravesac), by analysing root and leaf tissues. Rhizosphere bacterial dynamics and arbuscular mycorrhizal fungi (AMF) colonization have also been investigated during drought and recovery phases. Leaf ecophysiology and water relations were monitored over time, revealing diverse behaviours at severe stress (SS) and recovery (REC) conditions. Overall, combining anatomical, biochemical and gene expression data of stress-associated markers involved in ABA metabolism, osmolytes, antioxidant pathways and xylem features from both bionts, different ABA- or osmotic-dependent responses were observed at SS and REC for NE/3009 and NE/Gravesac combinations. Conversely, the NE/Kober5BB combination appeared to be in a primingstate, displaying higher root ABA content and AM colonization prior to stress imposition, along with a lower scion xylem area. These findings underscore the varied strategies employed by different grafted combinations in drought recovery, considering grapevines not as standalone entities, but rather as holobionts (with their inhabiting microbes) interacting collectively with the surrounding environment.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1,2, Nicola Belfiore1, Amedeo Moine2, Chiara Pagliarani2, Cristina Morabito3, Francesca Secchi3, Loredana Moffa1, Marco Sandrini1, Raffaella Balestrini2, Irene Perrone2, Giorgio Gambino2, Federica Gaiotti1, Danila Cuozzo2,3, Ivana Gribaudo2, Franco Mannini2, Claudio Lovisolo3 and Walter Chitarra1,2*

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

Rootstock, metabarcoding, gas exchange, AMF, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Terpenoids and norisoprenoids in italian red wines

AIM Terpene compounds are associated with floral notes and are characteristic of aromatic grape varieties such as Muscat (Jackson, 2008). They are generally considered to potentially contribute to the aroma of white wines. However, there is a growing interest towards the potential contribution of terpene compounds to the aroma of red wines. The aim of this work was to investigate the occurrence of different terpenes in red wines from Italian varieties. METHODS For this study wines from 11 mono-varietal Italian red wines from 12 regions were used (19 Sangiovese, 11 Nebbiolo, 10 Aglianico, 11 Primitivo, 10 Raboso del Piave, 9 Cannonau, 11 Teroldego, 3 Nerello, 9 Montepulciano, 7 Corvina). All samples were from vintage 2016 and none of them had been in contact with wood. A total of 19 terpenes and 7 norisoprenoids were analysed by mean of SPME-GC-MS analysis using a DVB-CAR-PDMS fiber. The wines were collected in the framework of the activities of the D-Wines (Diversity of Italian wines) project.

Read More

Classification and prediction of tannin botanical origin through voltammetry and machine learning approach

The classification of enological tannins has gained importance following the OIV’s requirement to include their botanical origin on product labels (OIV-OENO624-2022).

Read More

The complex response of Mediterranean viticultural systems to climate change: a case study from France and Australia

Climate change could put at risk viticultural areas situated at the hotter margins of Vitis vinifera growth climatic range. We focus on two such regions with a Mediterranean climate

Read More

Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Over the past 70 years, scientific literature has consistently illustrated the advantageous effects of arbuscular mycorrhiza fungi (AMF) on plant growth and stress tolerance. Recent reviews not only reaffirm these findings but also underscore the pivotal role of AMF in ensuring the sustainability of viticulture. In fact, various companies actively promote commercial inoculants based on AMF as biofertilizers or biostimulants for sustainable viticulture. However, despite the touted benefits of these products, the consistent effectiveness of AMF inoculants in real-world field conditions remains uncertain.

Read More

Adaptation et expression de l’encépagement et mode de conduite en différents terroirs de la région du Douro/vin de Porto

Ce travail a pour objet l’analyse des résultats agronomiques obtenus sur trois unités expérimentales du Centre d’Etudes Vitivinicoles du Douro (CEVDouro), localisées dans des écosystèmes différenciés de la Région du Douro/Vin de Porto, à différentes altitudes (130, 330 et 520 mètres) et à des expositions diversifiées (SE, N et W).

Read More