terclim by ICS banner
IVES 9 IVES Conference Series 9 Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Abstract

Viticulture is facing many challenges due to climate change effects with increasingly attention to save resources, such as water, considering that drought events have been predicted to dramatically increase over the next future. Thanks to the -omics techniques, research pushed forward knowledge to deepen facets of drought response in diverse grapevine-rootstock combinations. However, the regulatory mechanisms orchestrating adaptation strategies during drought and recovery in grafted grapevines need further exploration. Herein, we combined ecophysiological, biochemical and molecular approaches to unravel drought and recovery-induced changes in potted Nebbiolo (NE) plants grafted onto three different rootstocks (3309, Kober5BB, Gravesac), by analysing root and leaf tissues. Rhizosphere bacterial dynamics and arbuscular mycorrhizal fungi (AMF) colonization have also been investigated during drought and recovery phases. Leaf ecophysiology and water relations were monitored over time, revealing diverse behaviours at severe stress (SS) and recovery (REC) conditions. Overall, combining anatomical, biochemical and gene expression data of stress-associated markers involved in ABA metabolism, osmolytes, antioxidant pathways and xylem features from both bionts, different ABA- or osmotic-dependent responses were observed at SS and REC for NE/3009 and NE/Gravesac combinations. Conversely, the NE/Kober5BB combination appeared to be in a primingstate, displaying higher root ABA content and AM colonization prior to stress imposition, along with a lower scion xylem area. These findings underscore the varied strategies employed by different grafted combinations in drought recovery, considering grapevines not as standalone entities, but rather as holobionts (with their inhabiting microbes) interacting collectively with the surrounding environment.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1,2, Nicola Belfiore1, Amedeo Moine2, Chiara Pagliarani2, Cristina Morabito3, Francesca Secchi3, Loredana Moffa1, Marco Sandrini1, Raffaella Balestrini2, Irene Perrone2, Giorgio Gambino2, Federica Gaiotti1, Danila Cuozzo2,3, Ivana Gribaudo2, Franco Mannini2, Claudio Lovisolo3 and Walter Chitarra1,2*

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

Rootstock, metabarcoding, gas exchange, AMF, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Terroir effects on the response of Tempranillo grapevines to irrigation in four locations of Spain: agronomic performance and water relations

We report the effects of different drip irrigation treatments on the agronomic performance and water relations of Tempranillo grapevines, pruned to a bilateral cordon

Read More

Effect of non-Saccharomyces yeast and lactic acid bacteria on selected sensory attributes and polyphenols of Syrah wines

Consumers predominantly use visual, aromatic and texture cues as quality/preference indicators to describe olfactory sensations. In this study, the effect of micro-organism in wine production was investigated using analytical and sensory techniques to achieve relevant analytical characterisation. Selected anthocyanins, flavan-3-ols, flavonols and phenolic acids were quantified in Syrah wines using RP-HPLC-DAD. Standard oenological parameters were also measured. Syrah grape must was fermented with various combinations of Saccharomyces cerevisiae (S. cerevisiae) and non-Saccharomyces (Metschnikowia pulcherrima or Hanseniaspora uvarum) yeasts, which was followed by sequential inoculation of lactic acid bacteria (LAB) (Oenococcus oeni or Lactobacillus plantarum).

Read More

White wine light-strike fault: a comparison between flint and green bottles under the typical supermarket conditions

Consumer preference favors flint-glass wine bottles over the traditional dark-colored, but it is documented that light exposure can cause white wines to produce off-aromas and change in color, and consequently da[1]mage their quality. Aim of the study was to study the white wine shelf life under the typical supermarket conditions, by recording the light and temperature exposure, the colorimetric changes, and the light-strike fault. METHODS: One pilot experiment based on two white wines and eight-time points and one kinetic experiment based on four white wines and seven-time points were designed and realized using a typical supermarket shelf for 32 and 50 days, correspondently. By installing prototype sensors at 32 points of the shelf, the temperature, UV, IR, and Visible light exposure were registered every 10 min. Approximately 600 commercial wines, bottled in flint and colored glass, were used. The colorimetric changes of the wines were registered and the light-strike fault was evaluated.

Read More

Development of a new commercial phenolic analysis method for red grapes

Grape phenolic content is an important quality factor that influences the appearance and mouthfeel of premium red wines.

Read More

Genomic perspective of Lachancea thermotolerans in wine bioacidification

We have sequenced two commercial strains of Lachancea thermotolerans (Lt) from the company Lallemand: Laktia™ y Blizz™.

Read More