terclim by ICS banner
IVES 9 IVES Conference Series 9 Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Merging two genomes: a holistic approach to disentangle rootstock-mediated drought and recovery responses

Abstract

Viticulture is facing many challenges due to climate change effects with increasingly attention to save resources, such as water, considering that drought events have been predicted to dramatically increase over the next future. Thanks to the -omics techniques, research pushed forward knowledge to deepen facets of drought response in diverse grapevine-rootstock combinations. However, the regulatory mechanisms orchestrating adaptation strategies during drought and recovery in grafted grapevines need further exploration. Herein, we combined ecophysiological, biochemical and molecular approaches to unravel drought and recovery-induced changes in potted Nebbiolo (NE) plants grafted onto three different rootstocks (3309, Kober5BB, Gravesac), by analysing root and leaf tissues. Rhizosphere bacterial dynamics and arbuscular mycorrhizal fungi (AMF) colonization have also been investigated during drought and recovery phases. Leaf ecophysiology and water relations were monitored over time, revealing diverse behaviours at severe stress (SS) and recovery (REC) conditions. Overall, combining anatomical, biochemical and gene expression data of stress-associated markers involved in ABA metabolism, osmolytes, antioxidant pathways and xylem features from both bionts, different ABA- or osmotic-dependent responses were observed at SS and REC for NE/3009 and NE/Gravesac combinations. Conversely, the NE/Kober5BB combination appeared to be in a primingstate, displaying higher root ABA content and AM colonization prior to stress imposition, along with a lower scion xylem area. These findings underscore the varied strategies employed by different grafted combinations in drought recovery, considering grapevines not as standalone entities, but rather as holobionts (with their inhabiting microbes) interacting collectively with the surrounding environment.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1,2, Nicola Belfiore1, Amedeo Moine2, Chiara Pagliarani2, Cristina Morabito3, Francesca Secchi3, Loredana Moffa1, Marco Sandrini1, Raffaella Balestrini2, Irene Perrone2, Giorgio Gambino2, Federica Gaiotti1, Danila Cuozzo2,3, Ivana Gribaudo2, Franco Mannini2, Claudio Lovisolo3 and Walter Chitarra1,2*

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

Rootstock, metabarcoding, gas exchange, AMF, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Characterization of varieties named ‘Caiño’ cultivated from Northwest of Spain

The ‘Caiño’ cultivar was cultivated in Galicia (Northwestern Spain) before the invasion of grape phylloxera. Genetic diversity from this cultivar have been described and considered as originating in Galicia, ‘Caiño Tinto’, ‘Caiño Bravo’, ‘Caiño Redondo’, ‘Caiño Longo’ and ‘Caiño Blanco’.

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.

Interacción mesoclima-suelo en la calidad del vino de Cabernet-Sauvignon en las denominaciones de origen Priorato y Tarragona

Las condiciones heliotérmicas en España son en general favorables a alcanzar una elevada producción de azúcares en las bayas de prácticamente todas las variedades que se cultivan en nuestro país.

Iso-/anisohydric behavior in wine grapes may be a matter of soil moisture

There are claims that wine grape cultivars are either isohydric or anisohydric; the former maintaining, and the latter decreasing, their plant water status as soil moisture declines. However, available information is inconsistent. There are those that show an existence of a continuum in cultivar response to soil moisture rather than a distinct categorization. Others even show both behaviors in the same cultivar grown in different environments. In this study we investigated the behavior of 30 own rooted Vitis vinifera cultivars during successive drydown and rewatering cycles over two growing seasons in arid eastern Washington (<200 mm annual precipitation).

TCA – A status report on South African cork closures

Cork taint decreases the commercial value of wine as tainted wines are rejected by consumers. Although other compounds in wine and cork can also be responsible for causing a taint, 2,4,6-trichloroanisole (TCA) is regarded as the primary cause of cork taint. As cork taint is often used in marketing campaigns against natural cork closures,