terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Abstract

Vineyards are home to a myriad of microorganisms that interact with each other and with the vines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola, causing grapevine downy mildew. Others have a positive effect on vine health, such as disease biocontrol agents. These beneficial plant-microbe and microbe-microbe interactions have gained more attention in recent years because they could represent an alternative to the use of fungicides in viticulture. The aim of the present study is to identify bacterial and fungal taxa naturally present in vineyard soil and grapevine leaves and significantly more abundant in plots with low susceptibility to downy mildew (DM), susceptibility being defined by the intensity and frequency of DM symptoms over several years. Seven pairs of vineyard plots with contrasting susceptibility to DM were selected on the basis of a long-term epidemiological survey conducted in the Bordeaux region by the IFV. In each plot, we sampled young leaves (at phenological stage of 2-3 spreading leaves) and surface soil (top 5 cm) before the first fungicide treatments of the growing season. We used metabarcoding approaches to explore the entire microbial community of the samples. Up to 1974 and 769 taxonomic units of bacteria and fungi respectively were identified. Using differential abundance analyses, we could identify taxa that were significantly more abundant in plots of vines with low susceptibility to DM. As perspectives, the antagonistic activity of these taxa will be studied experimentally to develop microbial biocontrol of downy mildew and move viticulture towards pesticide-free viticulture.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Paola Fournier1,2,3*, Lucile Pellan1, Aarti Jaswa1,4, Jessica Vallance1, Emilie Chancerel2, Olivier Bonnard2, Marc Raynal5, Christian Debord5, Simon Labarthe2, Laurent Deliere1, François Delmotte1, Patrice This3, Corinne Vacher2

1INRAE, Bordeaux Sciences Agro, ISVV, SAVE, 33140 Villenave-d’Ornon, France
2INRAE, Univ Bordeaux, BioGeCo, 33610 Cestas, France
3INRAE, CIRAD, Univ Montpellier, Institut AGRO, AGAP institut, 34398 Montpellier, France
4Univ Bordeaux, UMR oenologie, INRAE, Bx INP, Bordeaux Sciences Agro, ISVV, 33882 Villenave d’Ornon ,France
5IFV, 33290 Blanquefort, France

Contact the author*

Keywords

Plasmopara viticola, phyllosphere, pest management, sustainable viticulture, grape-associated microorganisms

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of climate change on the aroma of red wines: a focus on dried fruit aromas

The volatile composition of grapes (free and bound forms) contributes greatly to the varietal aroma and quality of wines. Several agronomical parameters affect grapes composition and wine quality: maturity level at harvest, water status, and the intensity of sun exposure.

Read More

FOURIER TRANSFORM INFRARED SPECTROSCOPY IN MONITORING THE WINE PRODUCTION

The complexity of the wine matrix makes the monitoring of the winemaking process crucial. Fourier Transform Infrared Spectroscopy (FTIR) along with chemometrics is considered an effective analytical tool combining good accuracy, robustness, high sample throughput, and “green character”. Portable and non-portable FTIR devices are already used by the wine industry for routine analysis. However, the analytical calibrations need to be enriched, and some others are still waiting to be thoroughly developed.

Read More

Using nanopore skim-sequencing to characterise regional epigenetic variability in New Zealand Sauvignon Blanc

Recent advancements in genomic sequencing technologies have enabled more detailed and direct studies of DNA methylation, which can help characterise epigenetic variations in plants. The Grapevine Improvement team at the Bragato Research Institute is studying the use of Oxford Nanopore sequencing to identify epigenetic changes associated with environmental differences among clonally-propagated grapevines.

This study involved sequencing DNA from the same Sauvignon Blanc clone, sourced from diverse New Zealand viticultural regions, using the PromethION platform.

Read More

From grapes to sparking wines: Aromas evaluation in a vine-spacing

Aim: Wine aromatic profile is a combination of viticulture and oenological practices and it is related to character, quality, and consumer acceptance. Based on the competition between soil capacity and canopy development, and on the potential to produce sparkling wines at Caldas, in the south region of Minas Gerais (Brazil) (21°55´S and 46°23´W, altitude 1.100m), the aim of this work was the evaluation of the development of aromas (secondary metabolites) from grapes to sparkling wines in a vine-spacing experiment and whether the distance between the vines can influence the aromatic profile of the sparkling wines (final product). 

Read More

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

Read More