Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Usually the winemaker consider the grapeberry maturity as an actor of the wine quality. Grape seed are frequently used as a marker to assess the grape maturity. The first aim of this study is to obtain a better understanding of the impact of grape seed maturity on the grape seed and grape berry composition.

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance.

The Valpolicella area (Veneto Region, Italy) is famous for its high quality wines: Amarone and Recioto, both obtained from partial post-harvest dehydrated red grapes. The main cultivars used for these wines are Corvina and Corvinone. In this Region hundreds of years ago a particular training system (Pergola, cordon/cane with horizontal shoot-positioning) was developed. In the last 20 years the Guyot have been introduced in the area; now Pergola and Guyot are equally widespread in the Valpolicella area. In two different environmental conditions (hill and floodplain) two vineyards, one for each type of training system, were studied along two years (2011-2012).

Depuis près d’une quinzaine d’années, l’Appellation d’Origine Contrôlée (A.O.C.) Côtes du Rhône a engagé un vaste programme afin de mieux connaître et valoriser les potentialités des différents terroirs qui la composent.

This work aimed at setting up a multivariate and geostatistical methodology to map natural terroir units of the viticultural areas at the province scale (1:125,000).