terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Abstract

Vineyards are home to a myriad of microorganisms that interact with each other and with the vines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola, causing grapevine downy mildew. Others have a positive effect on vine health, such as disease biocontrol agents. These beneficial plant-microbe and microbe-microbe interactions have gained more attention in recent years because they could represent an alternative to the use of fungicides in viticulture. The aim of the present study is to identify bacterial and fungal taxa naturally present in vineyard soil and grapevine leaves and significantly more abundant in plots with low susceptibility to downy mildew (DM), susceptibility being defined by the intensity and frequency of DM symptoms over several years. Seven pairs of vineyard plots with contrasting susceptibility to DM were selected on the basis of a long-term epidemiological survey conducted in the Bordeaux region by the IFV. In each plot, we sampled young leaves (at phenological stage of 2-3 spreading leaves) and surface soil (top 5 cm) before the first fungicide treatments of the growing season. We used metabarcoding approaches to explore the entire microbial community of the samples. Up to 1974 and 769 taxonomic units of bacteria and fungi respectively were identified. Using differential abundance analyses, we could identify taxa that were significantly more abundant in plots of vines with low susceptibility to DM. As perspectives, the antagonistic activity of these taxa will be studied experimentally to develop microbial biocontrol of downy mildew and move viticulture towards pesticide-free viticulture.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Paola Fournier1,2,3*, Lucile Pellan1, Aarti Jaswa1,4, Jessica Vallance1, Emilie Chancerel2, Olivier Bonnard2, Marc Raynal5, Christian Debord5, Simon Labarthe2, Laurent Deliere1, François Delmotte1, Patrice This3, Corinne Vacher2

1INRAE, Bordeaux Sciences Agro, ISVV, SAVE, 33140 Villenave-d’Ornon, France
2INRAE, Univ Bordeaux, BioGeCo, 33610 Cestas, France
3INRAE, CIRAD, Univ Montpellier, Institut AGRO, AGAP institut, 34398 Montpellier, France
4Univ Bordeaux, UMR oenologie, INRAE, Bx INP, Bordeaux Sciences Agro, ISVV, 33882 Villenave d’Ornon ,France
5IFV, 33290 Blanquefort, France

Contact the author*

Keywords

Plasmopara viticola, phyllosphere, pest management, sustainable viticulture, grape-associated microorganisms

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Campania region grapevine patrimony: a determination of the heat requirement of 19 nearly all-native ultivars. Nine years of observations.

Nous avons peu d’informations sur les cépages cultivés dans la région de la Campania (sud de l’ltalie). En particulier insuffisant sont les études sur les besoins thermiques de tels cépages.

Read More

Spatial characterization of land use in the viticultural Maipo Valley (Chile), using aster image digital processing

L’entreprise viticole Concha y Toro S.A. gère environ 600 ha de vignes dans la Vallée du Maipo (A.O. Valle del Maipo). L’objectif est celui de caractériser spatialement ces vignobles et leur occupation du sol environnante. Le choix s’est porté vers la démarche de zonage viticole par l’analyse spatiale, utilisant des traitements d’images satellitaires afin d’avoir une vision synoptique de la zone à moindres coûts et délais. Un système d’informations géographiques (SIG) est construit à partir des données suivantes : cartes topographiques, géologique, fond cadastral numérique, images satellitaires. Un modèle numérique de terrain est par ailleurs construit à une résolution de 25 m à partir des cartes topographiques.

Read More

Physical-chemical and sensory characterization of wine made with the cultivar syrah produced in a double pruning system

In recent years, the consumption of fine wines in Brazil has increased significantly, a phenomenon that is also reflected in the expansion of production to new regions. In the brazilian southeast for example, the so-called “winter wines” are being produced, through management in two cycles, one of formation and one of production, with two prunings and one harvest per year, a technique known as double pruning, with vineyards established at altitudes close to or above 1,000 m above sea level.

Read More

Application of regenerative agriculture to viticulture: The REVINE project

Conventional viticulture improved the quality of production, but the economic costs can be unsustainable. Today, producers need to consider consumers’ demands for healthy, eco-friendly products. Institutions promote sustainable agriculture, with regenerative agriculture being the latest generation of methodologies focused on recovering losses and ensuring future sustainability. The revine project studies regenerative agricultural technology applied in mediterranean countries to provide precise indications for soil processing and effective vineyard treatments.

Read More

Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Among the macromolecules of enological interest in white wines, much attention has been paid to polysaccharides.

Read More