terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Biotic interactions 9 Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Mining microbiome data to identify antagonists of grapevine downy mildew (Plasmopara viticola)

Abstract

Vineyards are home to a myriad of microorganisms that interact with each other and with the vines. Some microorganisms are plant pathogens, such as the oomycete Plasmopara viticola, causing grapevine downy mildew. Others have a positive effect on vine health, such as disease biocontrol agents. These beneficial plant-microbe and microbe-microbe interactions have gained more attention in recent years because they could represent an alternative to the use of fungicides in viticulture. The aim of the present study is to identify bacterial and fungal taxa naturally present in vineyard soil and grapevine leaves and significantly more abundant in plots with low susceptibility to downy mildew (DM), susceptibility being defined by the intensity and frequency of DM symptoms over several years. Seven pairs of vineyard plots with contrasting susceptibility to DM were selected on the basis of a long-term epidemiological survey conducted in the Bordeaux region by the IFV. In each plot, we sampled young leaves (at phenological stage of 2-3 spreading leaves) and surface soil (top 5 cm) before the first fungicide treatments of the growing season. We used metabarcoding approaches to explore the entire microbial community of the samples. Up to 1974 and 769 taxonomic units of bacteria and fungi respectively were identified. Using differential abundance analyses, we could identify taxa that were significantly more abundant in plots of vines with low susceptibility to DM. As perspectives, the antagonistic activity of these taxa will be studied experimentally to develop microbial biocontrol of downy mildew and move viticulture towards pesticide-free viticulture.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Paola Fournier1,2,3*, Lucile Pellan1, Aarti Jaswa1,4, Jessica Vallance1, Emilie Chancerel2, Olivier Bonnard2, Marc Raynal5, Christian Debord5, Simon Labarthe2, Laurent Deliere1, François Delmotte1, Patrice This3, Corinne Vacher2

1INRAE, Bordeaux Sciences Agro, ISVV, SAVE, 33140 Villenave-d’Ornon, France
2INRAE, Univ Bordeaux, BioGeCo, 33610 Cestas, France
3INRAE, CIRAD, Univ Montpellier, Institut AGRO, AGAP institut, 34398 Montpellier, France
4Univ Bordeaux, UMR oenologie, INRAE, Bx INP, Bordeaux Sciences Agro, ISVV, 33882 Villenave d’Ornon ,France
5IFV, 33290 Blanquefort, France

Contact the author*

Keywords

Plasmopara viticola, phyllosphere, pest management, sustainable viticulture, grape-associated microorganisms

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Assessment of climate change impacts on water needs and growing cycle on grapevine in three DOs of NE Spain

This study assessed the suitability of grapevine growing in three DOs (Empordà, Pla de Bages and Penedès) of Catalonia (NE Spain) over the 21st century. For this purpose, an estimation of water needs and agroclimatic and phenological indicators was made. Climate change impacts were estimated at 1 km pixel resolution using temperature and precipitation projections from several general circulation models (GCM) and two climate change scenarios: RCP 4.5 (stabilization scenario) and RCP 8.5 (worst-case scenario). Potential crop evapotranspiration (following FAO procedure) and a daily water balance considering soil water holding capacity were used to estimate actual evapotranspiration of vines and, finally, water needs. Dynamics would be similar in the three DOs studied although the magnitude of impact differs. Water needs would be 2 and 3 times greater (ranging from 0 to more than 1500 m3/ha) than current water needs at both climate change scenarios. Moreover, blooming date would advance from 3 to 6 weeks, harvest date from 1 to 2.5 months, resulting in growing cycles from 10 to 80 days shorter. It should also be noted that frost risk would decrease from 6 to 76%, the number of days with temperatures above 30ºC during ripening would rise from 48 to 500% and tropical nights (minimum temperature >20ºC) at ripening would increase from 28 to 150%, depending on the scenario and the DOs. The impacts of climate change in the three DOs could result in significant limitations for grapevine cultivation and wine production if adaptive strategies are not applied. This result could serve as a basis for the design of specific and particular adaptation strategies to improve and maintain vineyards in the DOs studied and could be extrapolated to similar DOs and regions.

Stomatal behaviour of three minority grapevine varieties grown in the La Mancha region (Spain)

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Evaluation of the enological potential of red grapes in southern Brazil

The Campanha Gaúcha is located in the pampa biome and has unique characteristics, as it is the hottest producing region with the lowest volume of rain in Southern Brazil. Furthermore, the large extensions of flat or low-sloping areas, harsh winters and great sunshine during the ripening period, made this the second largest producer of fine wines in Brazil.

CHARACTERIZATION OF THE VOLATILE COMPOUNDS PROFILE OF COMMERCIAL GRAPPAS OBTAINED FROM THE POMACE OF AMARONE WINES

Grappa is a traditional Italian alcoholic beverage, with an alcohol content generally between 40-60% vol., obtained from the distillation of grape pomace used for the production of wine. Grappa are often aged in wooden barrels. There are various types of grappa: young, aromatic, aged, extra-aged depending on whether the distillate comes from aromatic vines or is aged in wooden barrels for shorter or longer periods. There is also flavored grappa if herbs, fruit or roots are added. All this makes it an extremely heterogeneous product both from an organoleptic and compositional point of view.

Production and technological characteristics of some French clones of the Chardonnay variety in Yugoslavia

L’observation est effectuée entre 1996 et 1998. L’expérience a commencé avec des clones numérotés: 75, 95, 96 et 227 de la variété Chardonnay. Le porte greffe est le Kober 5 BB. La forme de conduite est le cordon. La taille est longue. La densité de plantation est 3,5 x 1 mètre (2857 ceps par 1/ha).