terclim by ICS banner
IVES 9 IVES Conference Series 9 Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Abstract

Botrytis cinerea (Bc) is one of the main pathogens affecting the cultivated grapevine. A key role in grapevine tissue colonization is played by cell wall (CW) remodeling driven by CW Modifying Enzymes (CWMEs), expressed both by the host and the pathogen. Their action can impact CW integrity and trigger specific immune signaling, thus influencing Bc infection outcome. To further characterize the role of the CW in the grapevine response to Bc, two contrasting genotypes in their resistance to the fungus were artificially inoculated at full bloom. RNA-seq analysis and biochemical characterization of the CW and its modification in samples collected at 24 hours post-inoculation highlighted significant differences between genotypes. A gene set enrichment analysis indicated several over-represented categories upon infection, with a general down-regulation of those genes related to CW organization and pectin modification, mostly in the resistant genotype. Within the down-regulated CWMEs, Pectin Methyl-Esterase (PME) genes were found highly represented. Unlike, VviPME10 was significantly induced upon infection and was further characterized since its putative ortholog in Arabidopsis was associated with resistance to Bc. VviPME10promoter hosts several predicted binding sites for VviWRKY3, a defense-associated transcription factor, as highlighted by DAP-seq analysis. This evidence is under confirmation by luciferase assays. In addition, the artificial inoculation with Bc of leaves from six VviPME10 knock-out (KO) edited lines showed significantly larger lesion areas when compared to control plants at 5 dpi. Together, these results suggest that pectin modification, mediated by VviPME10, plays an important role in the grapevine response to Bc.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Jorge Lagrèze1,2, Antonio Santiago Pajuelo3, Lorenza Dalla Costa2, Daniele Coculo4, Gabriele Magon5, Luis Orduña3, Gaston Pizzio3, Chen Zhang3, Mickael Malnoy2, Vincenzo Lionetti4, Alessandro Vannozzi5, José Tomás Matus3, Claudio Moser2, Giulia Malacarne2*

1 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy
2 Research and Innovation Center, E. Mach Foundation, Via E. Mach 1, 38098, San Michele all’Adige (Trento), Italy
3 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
4 Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
5 Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16 – 35020 Legnaro (PD), Italy

Contact the author*

Keywords

Botrytis cinerea, transcriptomics, DAP-seq analysis, Cell wall, grapevine pectin methyl-esterase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The chemical composition of disease resistant hybrid grape cultivars and its impact on wine quality: an exploratory enquiry into sustainable wines

Disease resistant hybrid grape cultivars are now allowed in a number of EU wine PDOs, and are also accepted in a number of countries outside the EU. There is increasing interest in diseases resistant hybrid grape cultivars (RHGCs) because they allow for the production of healthy, high quality grapes with limited use of pesticides and the associated environmental and public health

MOVING FROM SULFITES TO BIOPROTECTION: WHICH IMPACT ON CHARDONNAY WINE?

Over the last few years, several tools have been developed to reduce the quantity of sulfites used during winemaking, including bioprotection. Although its effectiveness in preventing the development of spoilage microorganisms has been proven, few data are available on the impact of sulfite substitution by bioprotection on the final product. The objective of this study was therefore to characterize Chardonnay wines with the addition of sulfite or bioprotection in the pre-fermentation stage. The effects of both treatments on resulting matrices was evaluated at several scales: analysis of classical oenological parameters, antioxidant capacity, phenolic compounds, non-volatile metabolome and sensory profile.

Exploring the prevalence of esca-induced leaf symptoms in French vineyards and the role of climate: a national scale analysis

Esca, a severe trunk disease affecting vineyards, is caused by fungal pathogens that induce wood necrosis and decay, leaf symptoms, yield losses, and potentially a rapid death of the vine. The prevalence of this disease varies across years, regions, cultivars, and plot ages. Despite its significance in understanding and predicting dieback risk in different vineyards, the role of climate in trunk diseases remains a relatively unexplored research area. While some studies have demonstrated the impact of certain climatic conditions on the prevalence of the disease, they often focus on a limited number of plots and yield conflicting results.We conducted a statistical analysis, using a Bayesian approach on a national database comprising prevalence data of esca from over 500 different plots in France, spanning the years 2003 to 2022 and encompassing various cultivars.

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines.

Role of landscape diversity for biodiversity conservation in viticulture: life+ biodivine’s results

Nowadays biodiversity loss is considered as a prior environmental issue. Agricultural landscapes are particularly concerned, mainly through the specialization and intensification of farming activities which lead, at a larger scale, to landscape simplification. Landscape management would be a good means to halt biodiversity loss, but large-scale studies remain rare. The life+ project BioDiVine aims to understand biodiversity dynamics and promote sustainable conservation actions at this scale in viticulture.