Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Soil and climate maps at the 1:10000 scales exist for more than 12’000 ha of Swiss vineyards. The use of these maps as consulting tools for growers remains difficult due to the complexity

Grape growers around the world are seeking to reduce their reliance on herbicides. However, traditional alternatives to chemical weed control do not always integrate seamlessly into established vineyard operations. Employing nonchemical weed management often requires trellis alterations, purchasing or hiring new equipment, and depending on region, may significantly increase tractor passes required to reach desired level of weed control. Critical thinking and thoughtful strategies are necessary to minimize expenditures and maintain quality during the transition away from herbicides. In this trial, irrigation was installed underground in an effort to minimize water loss due to evaporation, better direct the water to the vines, and reduce weed growth in the difficult to control undervine area.

Context and purpose of the study. Climate change threatens traditional winegrowing regions, with about 90% of areas like southern France at risk by the end of the century due to heatwaves and droughts.

Three-dimensional functional-structural root architecture models, which decompose the root system architecture (RSA) into elementary developmental processes such as root emission, axial growth, branching patterns and tropism have become useful tools for (i) reconstructing in silico the spatial and temporal dynamics of root systems in a soil volume, (ii) analyzing their genotypic diversity and plasticity to the environment, and (iii) overcoming the bottleneck associated with their visualization and measurement in situ. Here, we present an original work on RSA phenotyping and modelling in grapevine. First, we developed 2D image-based analysis pipelines to quantify morphological and architectural traits in young grafts. Second, we parametrized and validated the 3D root model Archisimple on two rootstock genotypes (RGM, 1103P) grafted with V. vinifera Cabernet-Sauvignon and grown in different controlled conditions (rhizotrons, pots, tubes).

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions.