terclim by ICS banner
IVES 9 IVES Conference Series 9 Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Cell wall remodeling mediated by specific PME genes plays a role in grapevine response to Botrytis cinerea

Abstract

Botrytis cinerea (Bc) is one of the main pathogens affecting the cultivated grapevine. A key role in grapevine tissue colonization is played by cell wall (CW) remodeling driven by CW Modifying Enzymes (CWMEs), expressed both by the host and the pathogen. Their action can impact CW integrity and trigger specific immune signaling, thus influencing Bc infection outcome. To further characterize the role of the CW in the grapevine response to Bc, two contrasting genotypes in their resistance to the fungus were artificially inoculated at full bloom. RNA-seq analysis and biochemical characterization of the CW and its modification in samples collected at 24 hours post-inoculation highlighted significant differences between genotypes. A gene set enrichment analysis indicated several over-represented categories upon infection, with a general down-regulation of those genes related to CW organization and pectin modification, mostly in the resistant genotype. Within the down-regulated CWMEs, Pectin Methyl-Esterase (PME) genes were found highly represented. Unlike, VviPME10 was significantly induced upon infection and was further characterized since its putative ortholog in Arabidopsis was associated with resistance to Bc. VviPME10promoter hosts several predicted binding sites for VviWRKY3, a defense-associated transcription factor, as highlighted by DAP-seq analysis. This evidence is under confirmation by luciferase assays. In addition, the artificial inoculation with Bc of leaves from six VviPME10 knock-out (KO) edited lines showed significantly larger lesion areas when compared to control plants at 5 dpi. Together, these results suggest that pectin modification, mediated by VviPME10, plays an important role in the grapevine response to Bc.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Jorge Lagrèze1,2, Antonio Santiago Pajuelo3, Lorenza Dalla Costa2, Daniele Coculo4, Gabriele Magon5, Luis Orduña3, Gaston Pizzio3, Chen Zhang3, Mickael Malnoy2, Vincenzo Lionetti4, Alessandro Vannozzi5, José Tomás Matus3, Claudio Moser2, Giulia Malacarne2*

1 Center Agriculture Food Environment (C3A), University of Trento/Fondazione Edmund Mach, via E. Mach 1, 38098, San Michele all’Adige (TN), Italy
2 Research and Innovation Center, E. Mach Foundation, Via E. Mach 1, 38098, San Michele all’Adige (Trento), Italy
3 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
4 Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
5 Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16 – 35020 Legnaro (PD), Italy

Contact the author*

Keywords

Botrytis cinerea, transcriptomics, DAP-seq analysis, Cell wall, grapevine pectin methyl-esterase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effect of power ultrasound treatment on free and glycosidically-bound volatile compounds and the sensorial profile of red wines

AIM Aiming to explore the possibility of shortening red winemaking maceration times (1,2), this study presents the effect of the application of high-power ultrasounds to crushed grapes, at winery-scale, on the content of varietal volatile compounds (free and glycosidically-bound) in musts and on the overall aroma of wines.

Simulated climate change in a Mediterranean organic vineyard altered the plant physiology and decreased the vine production

This study focuses on investigating the effects of climate change on the plant physiology and berries of Vitis vinifera cv “Monastrell” in a commercial vineyard managed organically in Southeastern Spain (Jumilla, Murcia). For this purpose, open top chambers and rainout shelters were employed to simulate warming (~2-7 ºC, W) and rainfall reduction (~30%, RR) respectively. Additionally, a combination of both treatments (W+RR) was employed. Vines without either top chambers or rainout shelters were considered as control (C). The experiment was established in February of 2023. Predawn leaf water potential (measured using a pressure chamber), stomatal conductance (assessed with a porometer at mid-morning) and leaf chlorophyll and flavonoid content (measured using the Dualex® leaf clip sensor) were analyzed at veraison (5 months after the installation of structures).

Impact of pedoclimatical conditions on the precocity potential of vineyards in the canton of Geneva

Terroir studies are common nowadays but few have used precise pedoclimatic measures in order to evaluate the precocity potential. The objectives of this work were (i) to assess the effect of main terroir parameters (soil, climate and topography) influencing the phenological development of the vine, and (ii) to evaluate a geostatistic approach by using a high number of already existing plots (higher variability) to analyze the terroir parameters’ impact.

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Non-linear unmixing as an innovative tool to detect vine diseases in UAVs, airborned and satellite images: preliminary results

Vine diseases have a strong impact on vineyards sustainability, which in turns leads to strong economic consequences. Among those diseases, Flavescence dorée spreads quickly and is incurable, which led in France to the setup of a mandatory pest control implying the systematic use of pesticides and the prospection and uprooting of every infected plants. Remote sensing could be a very powerful tool to optimize prospection as it allows to produce quickly accurate maps over large areas. Recent studies have shown that high spatial resolution (10cm/pixel) multispectral images acquired from UAVs allow to map Flavescence dorée in vineyards using leaves discolorations [e.g. Albetis et al., Remote Sensing, 2017].