terclim by ICS banner
IVES 9 IVES Conference Series 9 Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Abstract

Esca complex is a disease affecting grapevine trunks, characterized by the colonization of the wood by xylem-residing fungi (Phaeomoniella chlamydosporaPhaeoacremonium minimum and Fomitiporia mediterranea), and posing significant risks to vineyard longevity since no efficient treatment is available. Despite its prevalence, the mechanisms beyond symptomatic manifestations like interveinal chlorosis and leaf necrosis remain unclear. Preliminary findings indicated a more pronounced metabolic reprogramming in fruits compared to vegetative organs and a putative impact on wine quality by using fruits from symptomatic grapevines. Here, we conducted metabolic profiling and untargeted/ targeted metabolomics to gather more insights into the molecular and biochemical mechanisms responsible for the onset of symptoms. Ultra-High Performance Liquid Chromatography (UHPLC-qTOF-MS/MS), Gas Chromatograph-Quadrupole Time of Flight Mass Spectrometry (GC-qTOF-MS/MS), and Liquid Cromatography (LC-MS/MS) enabled the identification of putative markers of symptomatology regarding hormonal regulation, primary and secondary metabolisms. Abscisic acid, jasmonates, and specific amino acids and sugars decrease in harvest-stage fruits from symptomatic grapevines, in contrast with the accumulation of a wide variety of phenylpropanoids (e.g., procyanidin B1, caftaric acid, resveratrol) among others. Secondary metabolism was more strongly remodelled indicating a partitioning of carbon allocated to defence-related metabolism. RNA extraction and sequencing are being conducted to integrate these metabolic results with molecular data. This study may contribute to developing a model regarding the development of Esca symptoms in an attempt to mitigate the worldwide impact of this complex disease.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Rute Amaro1*, Rita Pacheco2,3, Carla António4, Cecília Rego5, Lisete Sousa6, Paula Lopes1,7, Axel Mithöfer8, Ana Margarida Fortes1

1 BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal
2 Department of Chemical Engineering, ISEL—Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emídio Navarro, 1, 1959-007 Lisboa, Portugal
3 Centro de Química Estrutural, Institute of Molecular Sciences, Universidade de Lisboa, 1749-016 Lisboa, Portugal
4 Forest Research Centre (CEF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
5 LEAF – Linking Landscape, Environment, Agriculture and Food (LEAF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
6 Departamento de Estatística e Investigação Operacional e Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal
7 DNA & RNA Sensing Lab, University of Trás-os-Montes e Alto Douro, Department of Genetics and Biotechnology, School of Life Science and Environment, Vila Real, Portugal
8 Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany

Contact the author*

Keywords

Esca disease, Hormonal profiling, Primary metabolism, Phenylpropanoid pathways, RNA sequencing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Progetto di zonazione delle valli di Cembra e dell’Adige. Analisi del comportamento della varietà Pinot nero in ambiente subalpino

Nel 1990 la Cantina LA VIS ha intrapreso un progetto di zonazione dei terreni vitati allo scopo di acquisire le conoscenze scientifiche atte a consentire il miglioramento delle qualità dei prodotti. Tale progetto si è articolato su di una superficie di 2000 ettari ubicati lungo l’asta fluviale del fiume Adige da Trento a Salorno e del torrente Avisio da Lavis a Segonzano.

The evolution of italian vine nursery production over the past 30 years

Italy has a long history of viticulture and has become one of the world’s leading producers of vine propagation material. The Italian vine nursery industry is today highly qualified and has become highly competitive on a global scale. The quality of the material is guaranteed by compliance with European Union regulations, which have been in force since the second half of the 20th century and have subsequently been supplemented and updated.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.

Effect of Botrytis cinerea and esca on phenolic composition of berries and wines

This study showed that Botrytis cinerea could degrade the phenolic compounds by its enzymatic activity. It led to a diminution of skin’s anthocyanins from 20 % to 50 % and an increase level up to 40 % of individual proanthocyanins, 30 % of the %G and 25% of the %P.

Sparkling wines and atypical aging: investigating the risk of refermentation

Sparkling wine (SW) production entails a two-steps process where grape must undergoes a primary fermentation to produce a base wine (BW) which is then refermented to become a SW. This process allows for the development of a new physicochemical profile characterized by the presence of foam and a different organoleptic profile.