Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

La mise en valeur d’un terroir au travers du vin signifie dans un premier temps le respect du cahier des charges de l’A.O.C correspondante. Dans un second temps, elle sous-entend d’être à l’écoute des évolutions scientifiques, techniques et sociétales afin de satisfaire une production plus respectueuse de l’environnement et de la santé des hommes. Les recherches effectuées par l’Unité Vigne et Vin du centre INRA d’Angers ont débouché sur le concept d’UTB, Unité Terroir de Base (R.Morlat). UTB définit une aire de terrain ou le fonctionnement de la vigne est homogène en tous points.

Une démarche de cartographie des sols a été engagée en 2009 par l’interprofession des vins du Beaujolais à l’initiative des professionnels de la région. A fin 2015

Grapes (Vitis vinifera L.) are a fruit crop of high economic significance globally. Each grapevine cultivar is characterized by its distinctive grape aroma, affecting the wine quality. In several cultivars, the aroma is shaped by terpenoid (mono- and sesqui-terpenoids). Their profile is controlled by terpene synthases (TPS), which are part of a largely expanded gene family. How the variation in TPS copy number and sequence among cultivars determines terpenoid profiles of grapes remains largely unexplored. We annotated TPS in the haplotypes of seven genomes (Riesling, Albariño, Fiano, Gewürztraminer, Pinot Noir, Cabernet Sauvignon, and Viognier) using BLAST, GMAP, PFAM, and phylogenetic analyses. Further, TPS expression patterns and terpenoid accumulation during berry development and ripening were characterized using RNA-Seq and SPME/GC-MS platforms, respectively. Variation in TPS copy number exists among cultivars. Specifically, the TPS counts span a range of 251 to 150 for Riesling and Fiano, respectively, when considering combined haplotypes within each cultivar. Total terpenoid accumulation patterns throughout development were consistent among the five aromatic cultivars, marked by high concentrations in flowers, followed by a decline and subsequent rise during berry development and ripening, respectively. Conversely, non-aromatic cultivars exhibited no substantial increase in terpenoid concentration during ripening. Transcriptome and network analyses are currently employed to determine which TPS are expressed in the berry and determine the terpenoid profile of the specific cultivar. These findings shed light on the genomic determinants of grape aroma in major cultivars, and allow future studies focused on cultivar-specific responses of terpenoid biosynthesis to environmental stresses.

Sustainability is a key focus in viticulture, where managing abiotic and biotic stress presents a major challenge.

Extraction of anthocyanins and tannins have been studied for two grape varieties, Carignan and Grenache, two maturation levels and two vintages, in model solutions and in wines, using UHPLC-MS/MS in the MRM mode  and HPSEC.