terclim by ICS banner
IVES 9 IVES Conference Series 9 Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Abstract

Esca complex is a disease affecting grapevine trunks, characterized by the colonization of the wood by xylem-residing fungi (Phaeomoniella chlamydosporaPhaeoacremonium minimum and Fomitiporia mediterranea), and posing significant risks to vineyard longevity since no efficient treatment is available. Despite its prevalence, the mechanisms beyond symptomatic manifestations like interveinal chlorosis and leaf necrosis remain unclear. Preliminary findings indicated a more pronounced metabolic reprogramming in fruits compared to vegetative organs and a putative impact on wine quality by using fruits from symptomatic grapevines. Here, we conducted metabolic profiling and untargeted/ targeted metabolomics to gather more insights into the molecular and biochemical mechanisms responsible for the onset of symptoms. Ultra-High Performance Liquid Chromatography (UHPLC-qTOF-MS/MS), Gas Chromatograph-Quadrupole Time of Flight Mass Spectrometry (GC-qTOF-MS/MS), and Liquid Cromatography (LC-MS/MS) enabled the identification of putative markers of symptomatology regarding hormonal regulation, primary and secondary metabolisms. Abscisic acid, jasmonates, and specific amino acids and sugars decrease in harvest-stage fruits from symptomatic grapevines, in contrast with the accumulation of a wide variety of phenylpropanoids (e.g., procyanidin B1, caftaric acid, resveratrol) among others. Secondary metabolism was more strongly remodelled indicating a partitioning of carbon allocated to defence-related metabolism. RNA extraction and sequencing are being conducted to integrate these metabolic results with molecular data. This study may contribute to developing a model regarding the development of Esca symptoms in an attempt to mitigate the worldwide impact of this complex disease.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Rute Amaro1*, Rita Pacheco2,3, Carla António4, Cecília Rego5, Lisete Sousa6, Paula Lopes1,7, Axel Mithöfer8, Ana Margarida Fortes1

1 BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal
2 Department of Chemical Engineering, ISEL—Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emídio Navarro, 1, 1959-007 Lisboa, Portugal
3 Centro de Química Estrutural, Institute of Molecular Sciences, Universidade de Lisboa, 1749-016 Lisboa, Portugal
4 Forest Research Centre (CEF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
5 LEAF – Linking Landscape, Environment, Agriculture and Food (LEAF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
6 Departamento de Estatística e Investigação Operacional e Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal
7 DNA & RNA Sensing Lab, University of Trás-os-Montes e Alto Douro, Department of Genetics and Biotechnology, School of Life Science and Environment, Vila Real, Portugal
8 Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany

Contact the author*

Keywords

Esca disease, Hormonal profiling, Primary metabolism, Phenylpropanoid pathways, RNA sequencing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Typologie des paysages de vigne: un outil de planification

La culture de la vigne dessine un paysage rural original. En effet, de par ses qualités physiologiques, ses exigences agronomiques et les techniques qu’elle requiert, elle est à l’origine d’un portrait de nature sculpté, architecturé, parfois même comparé à l’art des jardins. A ce que l’on pourrait le cas échéant qualifier d’« art involontaire »

Flor yeast diversity and dynamics in biologically aged wines

Wine biological aging is characterized by the development of yeast strains that form a biofilm on the wine surface after alcoholic fermentation. These yeasts, known as flor yeasts, form a velum that protects the wine from oxidation during aging. Thirty-nine velums aged from 1 to 6 years were sampled from “Vin jaune” from two different cellars. We show for the first time that these velums possess various aspects in term of color and surface aspects. Surprisingly, the heterogeneous velums are mostly composed of one species, S. cerevisiae. Scanning electron microscope observations of these velums revealed unprecedented biofilm structures and various yeast morphologies formed by the sole S. cerevisiae species.

Non-targeted analysis of C13-norisoprenoid aroma precursors in Riesling

Significant wine aroma can be formed from non-volatile precursors that are linked to sugars, including but not limited to grape-derived monoterpene and C13-norisoprenoid glycosides.

The informative potential of remote and proximal sensing application on vertical- and overhead-trained vineyards in Northeast Italy

The application of remote and proximal sensing in viticulture have been demonstrated as a fast and efficient method to monitor vegetative and physiological parameters of grapevines. The collection of these parameters could be highly valuable to derive information on associated yield and quality traits in the vineyard. However, to leverage the informative potential of the sensing systems, a series of preliminary evaluations should be carried out to standardize working protocols for the specific features of a winegrowing area (e.g., pedoclimate, topography, cultivar, training system). This work aims at evaluating remote and proximal sensing systems for their performance and suitability to provide information on the vegetative, physiological, yield and qualitative aspects of vines and grapes as a function of different training systems in the Valpolicella wine region (Verona, Italy).

Sensory evaluation of grape berries: predictive power for sensory properties of Sauvignon blanc, Riesling and Pinot noir wines

Sensory analysis of grape berries is a common tool to evaluate the degree of grape maturation and to make sound picking decisions.