terclim by ICS banner
IVES 9 IVES Conference Series 9 Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Hormonal and associated metabolic changes in susceptible harvest-ripe grapes under asymptomatic and symptomatic Esca disease

Abstract

Esca complex is a disease affecting grapevine trunks, characterized by the colonization of the wood by xylem-residing fungi (Phaeomoniella chlamydosporaPhaeoacremonium minimum and Fomitiporia mediterranea), and posing significant risks to vineyard longevity since no efficient treatment is available. Despite its prevalence, the mechanisms beyond symptomatic manifestations like interveinal chlorosis and leaf necrosis remain unclear. Preliminary findings indicated a more pronounced metabolic reprogramming in fruits compared to vegetative organs and a putative impact on wine quality by using fruits from symptomatic grapevines. Here, we conducted metabolic profiling and untargeted/ targeted metabolomics to gather more insights into the molecular and biochemical mechanisms responsible for the onset of symptoms. Ultra-High Performance Liquid Chromatography (UHPLC-qTOF-MS/MS), Gas Chromatograph-Quadrupole Time of Flight Mass Spectrometry (GC-qTOF-MS/MS), and Liquid Cromatography (LC-MS/MS) enabled the identification of putative markers of symptomatology regarding hormonal regulation, primary and secondary metabolisms. Abscisic acid, jasmonates, and specific amino acids and sugars decrease in harvest-stage fruits from symptomatic grapevines, in contrast with the accumulation of a wide variety of phenylpropanoids (e.g., procyanidin B1, caftaric acid, resveratrol) among others. Secondary metabolism was more strongly remodelled indicating a partitioning of carbon allocated to defence-related metabolism. RNA extraction and sequencing are being conducted to integrate these metabolic results with molecular data. This study may contribute to developing a model regarding the development of Esca symptoms in an attempt to mitigate the worldwide impact of this complex disease.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Rute Amaro1*, Rita Pacheco2,3, Carla António4, Cecília Rego5, Lisete Sousa6, Paula Lopes1,7, Axel Mithöfer8, Ana Margarida Fortes1

1 BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal
2 Department of Chemical Engineering, ISEL—Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emídio Navarro, 1, 1959-007 Lisboa, Portugal
3 Centro de Química Estrutural, Institute of Molecular Sciences, Universidade de Lisboa, 1749-016 Lisboa, Portugal
4 Forest Research Centre (CEF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
5 LEAF – Linking Landscape, Environment, Agriculture and Food (LEAF), School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal
6 Departamento de Estatística e Investigação Operacional e Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal
7 DNA & RNA Sensing Lab, University of Trás-os-Montes e Alto Douro, Department of Genetics and Biotechnology, School of Life Science and Environment, Vila Real, Portugal
8 Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany

Contact the author*

Keywords

Esca disease, Hormonal profiling, Primary metabolism, Phenylpropanoid pathways, RNA sequencing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Terroir valorization strategies in a reformed denomination area: the Prosecco case study

Aims: This work summarizes some of the upmost recent studies and valorization strategies concerning the Prosecco wine production area. After the geographical denomination Prosecco (DO) was strongly reformed in 2009, the newborn DOCG (controlled and guaranteed DO) and DOC (controlled DO) areas have required different and specific strategies to promote and protect the value of their production.

Metabolomic insights into wine’s sensory identity: unveiling climate-driven changes in aroma composition

Wine, a sensitive and intricate agricultural product, is being affected by climate change, which accelerates grapevine phenological stages and alters grape composition and ripening. This influences the synthesis of key aroma compounds, shaping wine’s sensory attributes [1]. The complex aroma profile, resulting from compound interactions, presents a metabolomics challenge to identify these indicators and their environmental change responses, which is being addressed using diverse analytical techniques.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Synergistic effect of fumaric acid and chitosan on the inhibition of malolactic fermentation

During wine storage and aging, microorganisms capable of degrading malic acid in an undesirable manner can proliferate.