terclim by ICS banner
IVES 9 IVES Conference Series 9 Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Abstract

Soil is a reservoir of microorganisms playing important roles in biogeochemical cycles and interacting with plants whether in the rhizosphere or in the root endosphere. Through rhizodeposition, plants regulate their associated microbiome composition depending on the environment and plant factors, including genotypes. Since the phylloxera crisis, Vitis vinifera cultivars are mainly grafted onto American Vitis hybrids. Rootstocks play a pivotal role in the grapevine development, as the interface between the scion and the soil. Our work was carried out in the GreffAdapt plot, a unique experimental vineyard, including 55 rootstocks grafted with five different scions. Roots and rhizospheres from ten scion × rootstock combinations were collected in May 2021. Rhizosphere bacteria and fungi were quantified using cultivable approaches and qPCR. The communities of bacteria, fungi, and arbuscular mycorrhizal fungi in the rhizosphere and the roots were analyzed by Illumina sequencing of 16S rRNA gene, ITS and 28S rRNA gene, respectively. Our results highlight that both rootstock and scion genotypes influence the community structure in the rhizosphere and root compartments. The metabarcoding approach shows dissimilarities among bacterial and fungal communities depending on the rootstock or the scion genotype, suggesting that the two partners influence the microbial composition of the rhizosphere and the roots, as well as the putative functions of the microbiome (inferred using Picrust2 and FUNGuild). Finally, the roles of the microbiome in plant development and adaptation will be discussed by correlating its composition with plant phenotypic traits, as well as nutrient content of petioles and roots.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Vincent Lailheugue, Romain Darriaut, Anne Janoueix, Marine Morel, Joseph Tran, Elisa Marguerit, Virginie Lauvergeat*

EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, root system, metabarcoding, PICRUSt2, FUNGuild

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Typicality of Rioja wines: identification of sensory profiles for the three subregions of DOCa Rioja

Within the DOCa Rioja three main production areas are differentiated: Rioja Alta (RA), Rioja Alavesa (RAv) and Rioja Oriental (RO). They are three diverse territories with particular characteristics that are claimed to give rise to differentiated profiles. The present work aims at evaluating the sensory diversity of young commercial red wines in these three subregions. Therefore 30 young red wines (mainly Tempranillo and vintage 2021), ten from each subregion, were sensory described following a non-verbal free sorting task and a verbal free comment task by 32 well-established Rioja winemakers.

IMPACT OF ACIDIFICATION AT BOTTLING BY FUMARIC ACID ON RED WINE AFTER 2 YEARS

Global warming is responsible for a lack of organic acid in grape berries, leading to wines with higher pH and lower titrable acidity. The chemical, microbiological and organoleptic equilibriums are impacted by this change of organic acid concentration. It is common practice to acidify the wine in order to prevent these imbalances that can lead to wine defects and early spoilage. Tartaric acid (TA) is most commonly used by winemaker for wine acidification purposes. Fumaric acid (FA), which is authorized by the OIV in its member states for the inhibition of malolactic fermentation, could also be used as a potential acidification candidate since it has a better acidifying power than tartaric acid.

Lamp – a modern tool for the detection of fungal infections in the vineyard

AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry.

THE EFFECT OF BENTONITE FINING ON THE VOLATILE AND NON-VOLATILE PROFILE OF ITALIAN WHITE WINES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines. Macerations were of 7 days, except in the extended macerations that were of 15 days.

Atypical ageing defect in Pinot Blanc wines: influence of the grapevine production management.

Atypical ageing (ATA) is a wine aroma fault occurring in white wines characterised by an early loss of varietal aroma as well as nuances of wet mop, acacia blossom, shoe polish and dirty rag among others. 2-aminoacetophenone (2AAP) – a degradation product of indole-3-acetic acid (IAA) – has been described as the major odour-active compound and chemical marker responsible for this off-flavour. Depending on the aroma intensity of wines, its odour threshold varies from 0.5 to 10.5 μg/L. It seems that a stress reaction in the vineyard triggered by climatic, pedological and viticultural factors can ultimately cause ATA development in wines and therefore shorten their shelf-life.