terclim by ICS banner
IVES 9 IVES Conference Series 9 Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Abstract

Soil is a reservoir of microorganisms playing important roles in biogeochemical cycles and interacting with plants whether in the rhizosphere or in the root endosphere. Through rhizodeposition, plants regulate their associated microbiome composition depending on the environment and plant factors, including genotypes. Since the phylloxera crisis, Vitis vinifera cultivars are mainly grafted onto American Vitis hybrids. Rootstocks play a pivotal role in the grapevine development, as the interface between the scion and the soil. Our work was carried out in the GreffAdapt plot, a unique experimental vineyard, including 55 rootstocks grafted with five different scions. Roots and rhizospheres from ten scion × rootstock combinations were collected in May 2021. Rhizosphere bacteria and fungi were quantified using cultivable approaches and qPCR. The communities of bacteria, fungi, and arbuscular mycorrhizal fungi in the rhizosphere and the roots were analyzed by Illumina sequencing of 16S rRNA gene, ITS and 28S rRNA gene, respectively. Our results highlight that both rootstock and scion genotypes influence the community structure in the rhizosphere and root compartments. The metabarcoding approach shows dissimilarities among bacterial and fungal communities depending on the rootstock or the scion genotype, suggesting that the two partners influence the microbial composition of the rhizosphere and the roots, as well as the putative functions of the microbiome (inferred using Picrust2 and FUNGuild). Finally, the roles of the microbiome in plant development and adaptation will be discussed by correlating its composition with plant phenotypic traits, as well as nutrient content of petioles and roots.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Vincent Lailheugue, Romain Darriaut, Anne Janoueix, Marine Morel, Joseph Tran, Elisa Marguerit, Virginie Lauvergeat*

EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, root system, metabarcoding, PICRUSt2, FUNGuild

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of Ecklonia maxima seaweed extract on the vegetative, reproductive and microbiome in Vitis vinifera L. cv Cabernet-Sauvignon

Context and purpose of the study. Climate change is a major challenge in wine production. It results in erratic weather conditions which may lead to a reduction in grape yield and the subsequent grape and wine quality.

Use of minority grape varieties to mitigate climate change and achievement of balanced wines in Castilla y León (Spain)

Castilla y León is the third longest region in the European Union, having more than 85.000 vineyard hectares.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

ADDITION OF OAK WOOD ALTERNATIVE PRODUCTS: QUALITATIVE AND SENSORIAL EFFECTS FOR A WHITE WINE OF ALIGOTE

Wines matured in contact with wood are extremely popular with consumers all over the world. Oak wood allows the organoleptic characteristics of wine to be modified. Wines are enriched with volatile and non-volatile compounds extracted from the wood. The aromas extracted from oak wood contribute to the construction of the wine’s aromatic profile and the main polyphenols extracted can modify taste perceptions such as astringency and bitterness. All the compounds extracted from the wood thus contribute to the balance and quality of the wines.

Sensory changes in wines associated with the ripening of Grenache grapes from vineyards in different climatic zones

Climate change is introducing a high variability on grape ripening, causing uncertainty, excessive spending on pesticides and eventually frustrating results in terms of the quality of the vintage, with the increasingly frequent appearance of aromatic problems associated with overripeness, raisining and greenness, which sometimes only appear in bottled wines.