terclim by ICS banner
IVES 9 IVES Conference Series 9 Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Abstract

Soil is a reservoir of microorganisms playing important roles in biogeochemical cycles and interacting with plants whether in the rhizosphere or in the root endosphere. Through rhizodeposition, plants regulate their associated microbiome composition depending on the environment and plant factors, including genotypes. Since the phylloxera crisis, Vitis vinifera cultivars are mainly grafted onto American Vitis hybrids. Rootstocks play a pivotal role in the grapevine development, as the interface between the scion and the soil. Our work was carried out in the GreffAdapt plot, a unique experimental vineyard, including 55 rootstocks grafted with five different scions. Roots and rhizospheres from ten scion × rootstock combinations were collected in May 2021. Rhizosphere bacteria and fungi were quantified using cultivable approaches and qPCR. The communities of bacteria, fungi, and arbuscular mycorrhizal fungi in the rhizosphere and the roots were analyzed by Illumina sequencing of 16S rRNA gene, ITS and 28S rRNA gene, respectively. Our results highlight that both rootstock and scion genotypes influence the community structure in the rhizosphere and root compartments. The metabarcoding approach shows dissimilarities among bacterial and fungal communities depending on the rootstock or the scion genotype, suggesting that the two partners influence the microbial composition of the rhizosphere and the roots, as well as the putative functions of the microbiome (inferred using Picrust2 and FUNGuild). Finally, the roles of the microbiome in plant development and adaptation will be discussed by correlating its composition with plant phenotypic traits, as well as nutrient content of petioles and roots.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Vincent Lailheugue, Romain Darriaut, Anne Janoueix, Marine Morel, Joseph Tran, Elisa Marguerit, Virginie Lauvergeat*

EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, root system, metabarcoding, PICRUSt2, FUNGuild

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Relation between phenolic content, antioxidant capacity, oxygen consumption rate of diverse tannins

The work was aimed at comparing some analytical methods used to characterize oenological tannins and the measure of oxygen consumption rate (OCR), in order to provide oenologists with a rapid method to test the antioxidant capacity of tannin based products and a tool to choose the best suited product for each purpose.

Influence of processing parameters on aroma profile of conventional and ecological Cabernet-Sauvignon red wine during concentration by reverse osmosis

Wine aroma represents one of the most important quality parameter and it is influenced by various factors (viticulture and vinification techniques, climate or storage conditions etc.). Wines produced from conventionally and ecologically grown grapes of same variety have different chemical composition and aroma profile [1]. Aroma profile of wine can be also influenced by additional treatment of wine, such as concentration of wine by reverse osmosis (RO). Reverse osmosis represents a pressure-driven membrane separation technique that separates the initial wine on the retentate or concentrate that is retained on the membrane, and permeate that passes through it [2]. Wine permeate usually containes water, ethanol, acetic acid and several low molecular weight compounds that can pass through the membrane. This property enables the use of reverse osmosis membranes for wine concentration, partial dealcoholization, acetic acid or aroma correction [3,4].

Il Cabernet di Atina dal 1850 al giorni nostri: un esempio di valorizzazione del territorio

In the province of Frosinone from 1850 they are cultivated in some zones wine grape of French origin like Merlot, Cabernet franc Cabernet sauvignon, Sirah, Pinot noir. The insertion of these varieties was the work of Pasquale Visocchi in the great company of family “Fratelli Visocchi Proprietari” (F. V.P.).

Canopy management and trunk height elevation to adapt to climate change in Beaujolais and Savoie vineyards

In the Beaujolais and Savoie vineyards, climate change led to earlier phenological stages, more frequent episodes of elevated temperatures, and increased vine water deficit.

New markers for monitoring “fresh mushroom aroma” in wine: A dual approach using microbiological and chemical tools from the vineyard to winery–A synthesis of recent research advances

The ‘fresh mushroom off-flavour’ has been recognized by the wine industry as an emerging defect since the 2000s. For many years, this off-flavour was not specifically characterized and rather grouped under ‘earthy’ and ‘musty’ taints. However, it has become increasingly problematic due to its rising prevalence. In some vineyards, incidents of this off-flavour now occur as frequently as once every five years. This trend may be associated with climatic changes affecting regions that are more prone to warm and wet seasons.