terclim by ICS banner
IVES 9 IVES Conference Series 9 Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Abstract

Soil is a reservoir of microorganisms playing important roles in biogeochemical cycles and interacting with plants whether in the rhizosphere or in the root endosphere. Through rhizodeposition, plants regulate their associated microbiome composition depending on the environment and plant factors, including genotypes. Since the phylloxera crisis, Vitis vinifera cultivars are mainly grafted onto American Vitis hybrids. Rootstocks play a pivotal role in the grapevine development, as the interface between the scion and the soil. Our work was carried out in the GreffAdapt plot, a unique experimental vineyard, including 55 rootstocks grafted with five different scions. Roots and rhizospheres from ten scion × rootstock combinations were collected in May 2021. Rhizosphere bacteria and fungi were quantified using cultivable approaches and qPCR. The communities of bacteria, fungi, and arbuscular mycorrhizal fungi in the rhizosphere and the roots were analyzed by Illumina sequencing of 16S rRNA gene, ITS and 28S rRNA gene, respectively. Our results highlight that both rootstock and scion genotypes influence the community structure in the rhizosphere and root compartments. The metabarcoding approach shows dissimilarities among bacterial and fungal communities depending on the rootstock or the scion genotype, suggesting that the two partners influence the microbial composition of the rhizosphere and the roots, as well as the putative functions of the microbiome (inferred using Picrust2 and FUNGuild). Finally, the roles of the microbiome in plant development and adaptation will be discussed by correlating its composition with plant phenotypic traits, as well as nutrient content of petioles and roots.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Vincent Lailheugue, Romain Darriaut, Anne Janoueix, Marine Morel, Joseph Tran, Elisa Marguerit, Virginie Lauvergeat*

EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, root system, metabarcoding, PICRUSt2, FUNGuild

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sustainable yield management through fruitfulness and bunch architecture manipulation

Vineyards are highly variable and this variation is largely driven by environmental conditions and seasonal variation. For example, warm temperatures

Pruning vine-shoots as a new enological additive to differentiate and improve the quality of wines

The objective of these work was to demonstrate that toasted fragments of pruning vine-shoots added to the wines after fermentation provide them with differentiated aromatic notes and improve their quality.

Gevrey-Chambertin : les enjeux d’un territoire vitivinicole locale à l’échelle mondiale

An emblematic name of the burgundy wine region, a few kilometers from dijon, gevrey-chambertin stands out as a small wine town of international renown in the heart of a prestigious red wine vineyard listed as a unesco world heritage site.

MAPPING OF GAS-PHASE CO₂ IN THE HEADSPACE OF CHAMPAGNE GLASSES BY USING AN INFRARED LASER SENSOR UNDER STATIC TASTING CONDITIONS

From the chemical angle, Champagne wines are complex hydro-alcoholic mixtures supersaturated with dissolved carbon dioxide (CO₂). During the pouring process and throughout the several minutes of tasting, the headspace of a champagne glass is progressively invaded by many chemical species, including gas-phase CO₂ in large majority. CO₂ bubbles nucleated in the glass and collapsing at the champagne surface act indeed as a continuous paternoster lift for aromas throughout champagne or sparkling wine tasting [1]. Nevertheless, inhaling a gas space with a concentration of gaseous CO₂ close to 30% and higher triggers a very unpleasant tingling sensation, the so-called “carbonic bite”, which might completely perturb the perception of the wine’s bouquet.

WINE SWIRLING: A FIRST STEP TOWARDS THE UNLOCKING OF THE WINE’STASTER GESTURE

Right after the pouring of wine in a glass, a myriad of volatile organic compounds, including ethanol, overwhelm the glass headspace, thus causing the so-called wine’s bouquet [1]. Otherwise, it is worth noting that during wine tasting, most people automatically swirl their glass to enhance the release of aromas in the glass headspace [1]. About a decade ago, Swiss researchers revealed the complex fluid mechanics underlying wine swirling [2]. However, despite mechanically repeated throughout wine tasting, the consequences of glass swirling on the chemical space found in the headspace of wine glasses are still barely known.