terclim by ICS banner
IVES 9 IVES Conference Series 9 Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Roots and rhizosphere microbiota diversity is influenced by rootstock and scion genotypes: can this be linked to the development of the grafted plant?  

Abstract

Soil is a reservoir of microorganisms playing important roles in biogeochemical cycles and interacting with plants whether in the rhizosphere or in the root endosphere. Through rhizodeposition, plants regulate their associated microbiome composition depending on the environment and plant factors, including genotypes. Since the phylloxera crisis, Vitis vinifera cultivars are mainly grafted onto American Vitis hybrids. Rootstocks play a pivotal role in the grapevine development, as the interface between the scion and the soil. Our work was carried out in the GreffAdapt plot, a unique experimental vineyard, including 55 rootstocks grafted with five different scions. Roots and rhizospheres from ten scion × rootstock combinations were collected in May 2021. Rhizosphere bacteria and fungi were quantified using cultivable approaches and qPCR. The communities of bacteria, fungi, and arbuscular mycorrhizal fungi in the rhizosphere and the roots were analyzed by Illumina sequencing of 16S rRNA gene, ITS and 28S rRNA gene, respectively. Our results highlight that both rootstock and scion genotypes influence the community structure in the rhizosphere and root compartments. The metabarcoding approach shows dissimilarities among bacterial and fungal communities depending on the rootstock or the scion genotype, suggesting that the two partners influence the microbial composition of the rhizosphere and the roots, as well as the putative functions of the microbiome (inferred using Picrust2 and FUNGuild). Finally, the roles of the microbiome in plant development and adaptation will be discussed by correlating its composition with plant phenotypic traits, as well as nutrient content of petioles and roots.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Vincent Lailheugue, Romain Darriaut, Anne Janoueix, Marine Morel, Joseph Tran, Elisa Marguerit, Virginie Lauvergeat*

EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882 Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, root system, metabarcoding, PICRUSt2, FUNGuild

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sustainability as system innovation: sustainability as system innovation: a returnable system for glass wine bottles

Introduction increasing sustainability is essential and a societal challenge, requiring fundamental changes in behaviour and attitudes. This applies to both producers and consumers. For the wine industry in particular, such a change is a major challenge. An eip-agri research project is evaluating the introduction of a returnable glass system in the german wine industry as a key solution for increasing sustainability. Given the need for change associated with a returnable system, the project is theoretically grounded in systems innovation, as this approach provides solutions for complex, transformative change.

CONSUMER PERCEPTION OF INTERSPECIFIC HYBRID RED WINE COLOR IN RELATION TO ANTHOCYANIN PROFILE AND CHEMICAL COLOR PARAMETERS

Interspecific hybrid winegrapes are of growing interest in the context of climate change based on their disease resistance and cold hardiness. In addition to a need for increased understanding of their chemical composition, there is little empirical evidence on the consumer perception of non-vinifera wine. Phenolic compounds, and particularly color, play an important organoleptic and quality determination role in wine, but can vary significantly in interspecific hybrid wines compared to wines produced from Vitis vinifera cultivars [1, 2, 3]. Anecdotally, the variation in anthocyanin species, interactions, and concentrations in interspecific hybrids could result in a variance from“vinifera-like” wine color.

Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

As the frequency and intensity of drought events increase, understanding the mechanisms of plant resilience to water deficit is crucial. To maintain an appropriate plant yield, a common practice is the application of high amounts of fertilizers with negative environmental impacts. The single and combined effect of water deficit and nutrient availability, namely nitrogen (N) and potassium (K), in Vitis Vinifera L. cv. Cabernet Sauvignon and Grenache was evaluated. Two-year-old grapevine plants grafted on SO4 rootstock were transferred in pots under semi-environmental conditions. During the growing season, plants were either maintained well-watered (100% ETc) or subjected to a controlled water deficit irrigation (33% ETc).

High-power ultrasound for improving chromatic characteristics in wines. Does a varietal effect exist?

The use of high-power ultrasound (US) during the winemaking process has been extensively studied at laboratory scale in order to demonstrate its possible use to improve the extraction of compounds of interest. However, studies on semi-industrial and industrial scale are needed to confirm this positive effect, since the International Organization of Vine and Wine approved its industrial use in 2019 [1].

The importance of soil and geology in tasting terroir; a case history from the Willamette valley, Oregon

Wines differ from each other based on seven different factors: the type of grape; the bedrock geology and resulting soils; the climate; the soil hydrology; physiography of the site; the winemaker and the vineyard management techniques. The first five of these factors make up what the French call terroir, “the taste of the place”.