terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Abstract

Throughout centuries of anthropocentric breeding, plants have been selectively bred to enhance their quality traits and yield, often overlooking the importance of neglected attributes, like those involved in the interactions with beneficial microorganisms. This phenomenon led to an alteration in the distribution of photosynthetic products, shifting from defence mechanisms to growth, commonly described as ‘domestication syndrome’. Addressing the losses stemming from this condition is imperative just as unravelling the concealed communication between grapevines and beneficial microorganisms. To this aim, a Synthetic Community (SynCom) has been formulated, using grapevine endophytes and arbuscular mycorrhizal fungi, and  applied to potted cuttings of diverse rootstock genotypes. Plant development and physiological parameters were carefully monitored throughout an entire growing season. Root samples were collected for: i) DNA extraction and metabarcoding analysis to identify the root-associated microbiota and ii) RNA extraction for transcriptomic analysis. Concurrently, leaf and root samples were collected for targeted metabolomics, focusing on compounds involved in microbial recruitment, including, among others, coumarins, strigolactones, salicylic acid, and jasmonic acid.

Employing an innovative multi-omics approach, phenotypic, physiological, biochemical, and molecular data will be integrated to improve our understanding of the complex interaction within grapevine and its associated microbiota.This could result in new breeding programs which will also consider these traits as selection criteria, thereby preserving the ability of grapevine to recruit beneficial microorganisms. Furthermore, these results will provide useful information for the development of a more effective SynCom to augment holobiont resilience and thus to promote more sustainable agricultural practices.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Alberto Spada1,2*, Giuseppe Paradiso1, Marco Sandrini1, Chiara Biselli3, Teodora Basile1, Raffaella Balestrini4, Claudio Bonghi2, Luca Nerva1,4, Walter Chitarra1,4

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 University of Padova, Department of Agronomy, Food, Natural resources, Animals and Environment, Via dell’Università 16, 35020 Legnaro (PD), Italy
3 Research Centre for Forestry and Wood, Council for Agricultural Research and Economics (CREA-FL), Viale Santa Margherita 80, 52100, Arezzo, Italy
4 National Research Council of Italy – Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy

Contact the author*

Keywords

Rootstock, Domestication syndrome, Endophytes, Microbial recruiting, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Unconventional methods to delve deeper into the influence of temperature and nutrition on Chardonnay wine profiles

Temperature and yeast nutrition profoundly impact wine quality and sensory attributes by modulating yeast aroma production and release during fermentation. While temperature and nitrogen’s individual effects are well-studied, their combined influence, including nutrient type and addition timing, remains underexplored. hence, this study aimed to investigate the simultaneous effects of these factors on fermentation kinetics, aroma production and sensory profile, particularly in a Chardonnay wine production selected as a quite aromatically neutral base.

CHANGES IN METABOLIC FLUXES UNDER LOW PH GROWTH CONDITIONS: CAN THE SLOWDOWN OF CITRATE CONSUMPTION IMPROVE OENOCOCCUS OENI ACID-TOLERANCE?

Oenococcus oeni is the main Lactic Acid Bacteria responsible for malolactic fermentation, converting malic acid into lactic acid and carbon dioxide in wines. Following the alcoholic fermentation, this second fermentation ensures a deacidification and remains essential for the release of aromatic notes and the improvement of microbial stability in many wines. Nevertheless, wine is a harsh environment for microbial growth, especially because of its low pH (between 2.9 and 3.6 depending on the type of wine) and nutrient deficiency. In order to maintain homeostasis and ensure viability, O. oeni possesses different cellular mechanisms including organic acid metabolisms which represent also the major pathway to synthetize energy in wine.

Cover crops under-vine impact on grapevine performance and vineyard soil microorganisms is highly affected by edaphoclimatic conditions at a regional scale 

Soil management through cover crops can influence the cycle of nutrients, promote water infiltration, decrease erosion, and enhance the soil microbiota biodiversity, improving the grapevine performance. However, the area under the vines tends to be left bare by applying herbicides or tillage to avoid competition with the crop in semi-arid climates. Use of covers under-vine might be an alternative to these practices aiming at grapevine quality and soil health improvement. The aim of this research was to study the implications of soil management under the vines (cultivation and cover crops) on growth, yield, berry composition and soil microbial communities. A cover crop composed by a mixture of legumes was sown and compared with a control (cultivation), which includes frequent tillage to keep the soil bare, in three areas characterized by different edaphoclimatic conditions in the region of Navarra.

Root development and the performance of grapevines in response to natural as well as man‐made soil impediments

The majority of soils used for wine and table grape production in South Africa are notoriously shallow, i.e. they are restricting root penetration.

Reduce sulfur dioxide addition using a natural polymer chitosan phytate

Most oxidation reactions in wine require iron as a catalyst. The iron content of wine has decreased greatly in recent decades due to the use of low or no release cellar materials; however, in some cases it is still necessary to adopt winemaking practices to remove excess iron from wine, prevent its oxidation, and be able to reduce the addition of sulfur dioxide and other antioxidants.