Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Certain yeast species belonging to the Pichia genus are known to form a distinctive film on grape must and wine. In a mixed-culture type fermentation, Pichia spp. (P. kluyveri in particular) are known to impart beneficial oenological attributes. In this study, we report on an easy isolation method of Pichia spp. from grape must by exploiting their film-forming capacity on media containing 10% ethanol. We isolated and identified two Pichia species, namely Pichia kudriavzevii and Pichia kluyveri, and subsequently co-inoculated them with Saccharomyces cerevisiae to ferment Gewürztraminer musts. Noteworthy differences included a significant increase in the 2-phenethyl acetate levels with the P. kluyveri co-fermentation and a general increase in ethyl esters with the P. kudriavzevii co-fermentation. Both Pichia co-inoculations yielded higher levels of glycerol in the final wines. Based on all the wine parameters we tested, the P. kluyveri strain that was isolated performed similarly to a commercial P. kluyveri strain.

The brown marmorated stink bug (BMSB, Halyomorpha halys Stal) is an invasive pentatomid native to eastern Asia that is spreading rapidly worldwide, notably through human-mediated activities. Globally, it was reported in the USA, Canada, Italy, Hungary, and other European countries. BMSB has a broad host range that includes over 170 plants, many of agricultural importance, including various fruit, vegetables, row crops, and ornamentals. When present in the vineyard, the pest can affect yield and quality by directly feeding on berries resulting in fruit collapse and necrosis. Additional damage occurs when BMSB are carried into the winery within the grape clusters. The presence of BMSB during wine processing can affect juice and wine quality through the release of volatile compounds produced as a stress response. The major secretes compounds are tridecane and trans-2-decenal. Tridecane is an odorless compound and its effect on wine quality is currently unknown. Trans-2-decenal is an unsaturated aldehyde considered to be the main component of BMSB taint with strong green, coriander, and musty-like aromas. Its threshold value in wine was estimated at about 5 µg/L.

Parallèlement à la connaissance des aptitudes viticoles, le terroir témoigne d’une identité locale, d’une spécificité des conditions de productions et d’une originalité des lieux.

AIM: Previous research on the fruity character of red wines highlighted the role of esters [1]. Literature provides evidence that, besides these esters, other compounds that are not necessarily volatiles may have an important impact on the overall fruity aroma of wine, contributing to a masking effect [2][3]. The goal of this work was to assess the olfactory consequences of a mixture between esters and proanthocyanidic tannins, through sensory and physico-chemical approaches.

Uno degli aspetti più importanti nel commercio internazionale dei vini a denominazione è quello del riconoscimento dei diritti di esclusiva garantiti sui e dal territorio geografico d’o­rigine. Al fine di cautelarsi nei confronti della sempre più agguerrita concorrenza mondiale, è opportuno adottare adeguate protezioni ufficiali e legali delle denominazioni che possono derivare sia dalla “naturalità” del prodotto stesso che dalla “originalità” più particolare.