terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Abstract

Throughout centuries of anthropocentric breeding, plants have been selectively bred to enhance their quality traits and yield, often overlooking the importance of neglected attributes, like those involved in the interactions with beneficial microorganisms. This phenomenon led to an alteration in the distribution of photosynthetic products, shifting from defence mechanisms to growth, commonly described as ‘domestication syndrome’. Addressing the losses stemming from this condition is imperative just as unravelling the concealed communication between grapevines and beneficial microorganisms. To this aim, a Synthetic Community (SynCom) has been formulated, using grapevine endophytes and arbuscular mycorrhizal fungi, and  applied to potted cuttings of diverse rootstock genotypes. Plant development and physiological parameters were carefully monitored throughout an entire growing season. Root samples were collected for: i) DNA extraction and metabarcoding analysis to identify the root-associated microbiota and ii) RNA extraction for transcriptomic analysis. Concurrently, leaf and root samples were collected for targeted metabolomics, focusing on compounds involved in microbial recruitment, including, among others, coumarins, strigolactones, salicylic acid, and jasmonic acid.

Employing an innovative multi-omics approach, phenotypic, physiological, biochemical, and molecular data will be integrated to improve our understanding of the complex interaction within grapevine and its associated microbiota.This could result in new breeding programs which will also consider these traits as selection criteria, thereby preserving the ability of grapevine to recruit beneficial microorganisms. Furthermore, these results will provide useful information for the development of a more effective SynCom to augment holobiont resilience and thus to promote more sustainable agricultural practices.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Alberto Spada1,2*, Giuseppe Paradiso1, Marco Sandrini1, Chiara Biselli3, Teodora Basile1, Raffaella Balestrini4, Claudio Bonghi2, Luca Nerva1,4, Walter Chitarra1,4

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 University of Padova, Department of Agronomy, Food, Natural resources, Animals and Environment, Via dell’Università 16, 35020 Legnaro (PD), Italy
3 Research Centre for Forestry and Wood, Council for Agricultural Research and Economics (CREA-FL), Viale Santa Margherita 80, 52100, Arezzo, Italy
4 National Research Council of Italy – Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy

Contact the author*

Keywords

Rootstock, Domestication syndrome, Endophytes, Microbial recruiting, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

What practices in the vineyard lead to the production of wines that consistently win medals?

High quality wines start in the vineyard however little is known about the role vineyard management practices play in this quality outcome. Gold medals and well-known regionality increase consumer preference for purchasing a wine. An increase in the former will certainly also drive an increase in the latter and therefore practices in production that consistently lead to gold medal winning wines will improve both the marketability of the region and its products. It is argued that vinification is the main driver of wine quality and in fact, the presence of some oak compounds is a well-known consumer and expert mark of quality. However, only select wines are vinified in oak and therefore the original grape quality at the winery door must in fact drive all further downstream vinification decisions.

Transition metals and light-dependent reactions: application of a response surface methodology approach

Light-induced reactions can be responsible for detrimental changes of white and rosé wines. This is associated to the photo-degradation of riboflavin (RF) and of methionine (Met) causing the appearance of light-struck taste (LST).

Rapid quantification of higher alcohols in wine, port wine and brandy by HS-GC-FID

In response to the growing demand for rapid, precise, and efficient methods of quantifying volatile compounds in alcoholic beverages, this study presents a novel approach for the determination of higher alcohols in wine, port wine, and brandy.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Evaluation of interception traps for capture of Xylotrechus arvicola (Coleoptera: Cerambycidae) in vineyards varieties from Protected Denomination of Origin León

Xylotrechus arvicola (Coleoptera: Cerambycidae) is a pest in vineyards (Vitis vinifera) in the main Spain wine-producing regions with Protected Denomination of Origin (PDO). The action of the larvae, associated to the spreading of wood fungi, causes damage especially in important varieties of V. vinifera. X. arvicola females lay eggs concentrated in cracks or under the rhytidome in the wood vines, which allows the emerging larvae to get into the wood and make galleries inside the plant being then necessary to prune intensively or to pull up the bored plants (1). The objective of the study was to evaluate captures of X. arvicola insects in five varieties of V. vinifera in PDO León.