terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Abstract

Throughout centuries of anthropocentric breeding, plants have been selectively bred to enhance their quality traits and yield, often overlooking the importance of neglected attributes, like those involved in the interactions with beneficial microorganisms. This phenomenon led to an alteration in the distribution of photosynthetic products, shifting from defence mechanisms to growth, commonly described as ‘domestication syndrome’. Addressing the losses stemming from this condition is imperative just as unravelling the concealed communication between grapevines and beneficial microorganisms. To this aim, a Synthetic Community (SynCom) has been formulated, using grapevine endophytes and arbuscular mycorrhizal fungi, and  applied to potted cuttings of diverse rootstock genotypes. Plant development and physiological parameters were carefully monitored throughout an entire growing season. Root samples were collected for: i) DNA extraction and metabarcoding analysis to identify the root-associated microbiota and ii) RNA extraction for transcriptomic analysis. Concurrently, leaf and root samples were collected for targeted metabolomics, focusing on compounds involved in microbial recruitment, including, among others, coumarins, strigolactones, salicylic acid, and jasmonic acid.

Employing an innovative multi-omics approach, phenotypic, physiological, biochemical, and molecular data will be integrated to improve our understanding of the complex interaction within grapevine and its associated microbiota.This could result in new breeding programs which will also consider these traits as selection criteria, thereby preserving the ability of grapevine to recruit beneficial microorganisms. Furthermore, these results will provide useful information for the development of a more effective SynCom to augment holobiont resilience and thus to promote more sustainable agricultural practices.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Alberto Spada1,2*, Giuseppe Paradiso1, Marco Sandrini1, Chiara Biselli3, Teodora Basile1, Raffaella Balestrini4, Claudio Bonghi2, Luca Nerva1,4, Walter Chitarra1,4

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 University of Padova, Department of Agronomy, Food, Natural resources, Animals and Environment, Via dell’Università 16, 35020 Legnaro (PD), Italy
3 Research Centre for Forestry and Wood, Council for Agricultural Research and Economics (CREA-FL), Viale Santa Margherita 80, 52100, Arezzo, Italy
4 National Research Council of Italy – Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy

Contact the author*

Keywords

Rootstock, Domestication syndrome, Endophytes, Microbial recruiting, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effects of abscisic acid treatment on Vitis vinifera L. Savvatiano and Mouchtaro grapes and wine characteristics

Grapes development is determined by grape cultivar and vineyard climatic conditions and consequently affecting the phenolic and aroma on grapes and wines. Abscisic Acid (ABA) plays a key role in the promotion of fruit ripening and fruit anthocyanin content. Herein, we report the impact of ABA to grape ripening and wine quality.

Read More

First disclosure of eugenol precursors in Vitis genus: analytical development and quantification

The main aim of this work was to develop an analytical method to disclosure the
molecular form of eugenol precursor. Indeed eugenol is an important contributor to
Armagnac spirits typicity made with Baco blanc.

Read More

Multidisciplinary assessment of selective harvesting in the Colli Piacentini wine district

Within-field variability can be managed through Precision Viticulture (PV) protocols aiming at identifying homogeneous zones and addressing site-specific operations including selective harvesting (SH). Several authors demonstrated SH profitability in extensive viticulture while few information is available within the Italian context.

Read More

New understanding on sulfites reactivity in wine

Sulfur dioxide is widely used during winemaking as an antioxidant and antimicrobial agent. Bisulfite (HSO3−), the predominant form of SO2 at wine pH, reacts with several wine components forming sulfonated adducts.

Read More

Digitising the vineyard: developing new technologies for viticulture in Australia 

New and developing technologies, that provide sensors and the software systems for using and interpreting them, are becoming pervasive through our lives and society. From smart phones to cars to farm machinery, all contain a range of sensors that are monitored automatically with intelligent software, providing us with the information we need, when we need it. This technological revolution has the potential to monitor all aspects of vineyard activity, assisting growers to make the management choices they need to achieve the outcomes they want. For example, a future vineyard may possess automated imaging that generates a three dimensional model of the vine canopy, highlighting differences from the desired structure and how to use canopy management to improve fruit composition, or generates maps with yield estimates and measurements of berry composition throughout the growing season.

Read More