terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Exploring the inner secrets of grapevine: a journey through plant-microbe interactions

Abstract

Throughout centuries of anthropocentric breeding, plants have been selectively bred to enhance their quality traits and yield, often overlooking the importance of neglected attributes, like those involved in the interactions with beneficial microorganisms. This phenomenon led to an alteration in the distribution of photosynthetic products, shifting from defence mechanisms to growth, commonly described as ‘domestication syndrome’. Addressing the losses stemming from this condition is imperative just as unravelling the concealed communication between grapevines and beneficial microorganisms. To this aim, a Synthetic Community (SynCom) has been formulated, using grapevine endophytes and arbuscular mycorrhizal fungi, and  applied to potted cuttings of diverse rootstock genotypes. Plant development and physiological parameters were carefully monitored throughout an entire growing season. Root samples were collected for: i) DNA extraction and metabarcoding analysis to identify the root-associated microbiota and ii) RNA extraction for transcriptomic analysis. Concurrently, leaf and root samples were collected for targeted metabolomics, focusing on compounds involved in microbial recruitment, including, among others, coumarins, strigolactones, salicylic acid, and jasmonic acid.

Employing an innovative multi-omics approach, phenotypic, physiological, biochemical, and molecular data will be integrated to improve our understanding of the complex interaction within grapevine and its associated microbiota.This could result in new breeding programs which will also consider these traits as selection criteria, thereby preserving the ability of grapevine to recruit beneficial microorganisms. Furthermore, these results will provide useful information for the development of a more effective SynCom to augment holobiont resilience and thus to promote more sustainable agricultural practices.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Alberto Spada1,2*, Giuseppe Paradiso1, Marco Sandrini1, Chiara Biselli3, Teodora Basile1, Raffaella Balestrini4, Claudio Bonghi2, Luca Nerva1,4, Walter Chitarra1,4

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, (TV), Italy
2 University of Padova, Department of Agronomy, Food, Natural resources, Animals and Environment, Via dell’Università 16, 35020 Legnaro (PD), Italy
3 Research Centre for Forestry and Wood, Council for Agricultural Research and Economics (CREA-FL), Viale Santa Margherita 80, 52100, Arezzo, Italy
4 National Research Council of Italy – Institute for Sustainable Plant Protection (CNR-IPSP), Strada delle Cacce 73, 10135 Torino (TO), Italy

Contact the author*

Keywords

Rootstock, Domestication syndrome, Endophytes, Microbial recruiting, Multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Influence of the irrigation period in Tempranillo grapevine, under the edaphoclimatic conditions of the Duero river valley

Irrigation of vineyards is a matter of controversial arguments at areas of high quality wine production. Besides, the effects of the water in the plant are closer related to the water availability than to the irrigation regime.

Physicochemical parameters of juices made from different grape varieties in the 2019 and 2020 Harvests of Rio Grande do Sul

This study evaluated the physicochemical parameters of grape juices produced in the serra gaúcha from the 2019 and 2020 harvests. To do this, 43 juice samples were analyzed, and divided into four distinct categories: juices made exclusively from bordô grapes (sb), juices made from bordô and niágara grapes (sbn), juices combining bordô and isabel grapes, and juices made from cuts of several grape varieties.

Separation and elucidation of ethylidene-bridged catechin oligomers using preparative-HPLC and NMR

During wine aging, small amounts of oxygen are absorbed and initiate a cascade of oxidation reactions. These aging reactions create many products including ethylidene-bridged oligomers and polymers of endogenous polyphenols, like flavan-3ols.

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).

Determination of Aroma Compounds in Grape Mash under Conditions of Tasting by On-line Near-Infrared Spectroscopy

The production of high-quality wines requires the use of high-quality grapes. Some compounds originating from grapes may negatively influence the odour and flavour of the resulting wine in their original form or as precursors for off-odours and –flavours. Therefore, a rapid evaluation of the grapes directly upon receival at the winery is advantageous. Up to now, grape aroma is mainly evaluated by tasting, however, this leads to subjective results. The use of near-infrared (NIR) spectroscopy allows a rapid, objective and destruction-free analysis without previous sample preparation. Moreover, the measurement can be integrated into an existing process without additional sampling.