Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

Irrigation of vineyards is a matter of controversial arguments at areas of high quality wine production. Besides, the effects of the water in the plant are closer related to the water availability than to the irrigation regime.

The plasticity of grapevines in response to diverse growing conditions is influenced, among other factors, by the extent to which the roots explore the soil and the ability to accumulate and retrieve water and nutrients.
Newly planted grapevines, in particular, face challenges due to limited resources. The young plant’s ability for a fast and intensive penetration of the soil is vital in periods of water scarcity. The selection of an appropriate, site-specific rootstock significantly impacts both, the quality of the fruit produced and the economic success of the wine estate.

Soil management through cover crops in the lines of the vineyards is a common practice in viticulture, since it improves the characteristics of the soil. It has been shown that the cover crops can influence the cycle of nutrients, promote infiltration, decrease erosion, and enhance the soil microbiota biodiversity improving the grapevines. However, the area under the vines tends to be left bare by applying herbicides or tillage to avoid competition with the crop in hot climates. The use of cover crops under the vines might be a plausible alternative to the use of herbicides or cultivation, improving grapevine quality and soil characteristics. The aim of this research was to study the implications of different management of the soil under the vines (herbicide, cultivation or cover crops) on grapevine growth, water and nutritional status and belowground microbial communities.

A good knowledge of the soil physicochemical properties, as well as its ability to retain and put the necessary water available to the plants, is essential when it comes at the design of an irrigation plan.

AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry.