Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

Context description and research question: an increasing number of farmers are considering the impact of conservation practices on soil health to guide sustainable management of vineyards. Understanding impacts of soil management on soil organic carbon (SOC) is one lever for adoption of agroecological practice with potential to help maintain or improve soil health while building SOC stocks to mitigate climate change (Amelung et al., 2020).

The inherent variability of Nature poses challenges for researchers to draw clear conclusions from field experiments. Identifying and assessing adaptations to climate change requires agronomic field trials.

Wine cellars are affected by seasonally fluctuating workloads and face challenges especially in the harvest period connected to the required timely processing of the harvested grapes.

Biofilms are sessile microbial communities whose lifestyle confers specific properties. They can be defined as a structured community of bacterial cells enclosed in a self-produced polymeric matrix and adherent to a surface and considered as a method of immobilisation. Immobilised microorganisms offer many advantages for industrial processes in the production of alcoholic beverages and specially increasing cell densities for a better management of fermentation rates.

Viticulture is threatened by the environmental modification caused by climate change. Higher temperatures determine an acceleration of the ripening process, which can be detrimental to wine quality. In the mediterranean area, heat waves are also increasingly frequent, with consequent blocking of the vegetative activity of the vines and increased susceptibility to sunburn damage. thus, adaptation strategies are necessary to reduce stress and improve the quality of grape production. Amongst the various techniques available, shading nets represent an interesting alternative for their effects on canopy microclimate (i.e., reduction of photosynthetic activity, improvement of water use efficiency, and slowing down in the ripening process).