Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

Grapevine downy mildew is a disease of foliage caused by Oomycete Plasmopara viticola an endoparasite that develops inside grapevine organs and can infect virtually every green organ. Downy mildew is one of the most destructive diseases in wine-growing regions, drastically reducing yield and fruit quality. Traditional manual disease detection relies on farm experts. Human field scouting has been widely used for monitoring the disease progress, however, is costly, laborious, subjective, and often imprecise.

Hydroxytyrosol (HT) is a phenolic compound present in olives, virgin olive oil and wine. HT has attracted great scientific interest due to its biological activities which have been related with the ortho-dihydroxy conformation in the aromatic ring. In white and red wines, HT has been detected at concentrations ranging from 0.28 to 9.6 mg/L and its occurrence has been closely related with yeast metabolism of aromatic amino acids by Ehrlich pathway during alcoholic fermentation. One of the most promising properties of this compound is the neuroprotective activity against pathological mechanisms related with neurode-generative disorders including Alzheimer’s and Parkinson’s disease.

The postharvest dehydration of grapes is a traditional practice to obtain wines with unique traits (e.g., sweet, dry/reinforced).

Increasing pressures from both biotic and abiotic stresses threaten grapevine health and vineyard productivity worldwide. Arbuscular mycorrhizal fungi (AMF) inoculants have been shown to offer a promising bio-based alternative to mitigate abiotic stress and pathogen attack by enhancing nutrient uptake, water relations, and plant defence mechanisms.

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.