Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

L’objectif de cette recherche est le zonage climatique des périodes viticoles de l’année dans la Vallée du São Francisco, région brésilienne productrice de vins située en climat tropical semi-aride. Dans cette région, la production peut être échelonnée sur tous les mois de l’année.

Anticipation in the possible responses of grapevines to environmental variations is key to adjust field work in view of a more effective management. This idea has been the driving force behind the current work, which seeks to understand the interaction patterns of the vine with its habitat throughout the growing cycle.

Grapevine (Vitis vinifera L.) exhibits a high level of genetic and phenotypic diversity among the approximately 6000 cultivars recorded. This perennial crop is highly vulnerable to numerous fungal diseases, including esca, which is a complex vascular pathology that poses a significant threat to the wine sector, as there is currently no cost-efficient curative method[1]. In this context, an effective approach to mitigate the impact of such diseases is by leveraging the crop’s genetic diversity. Indeed, susceptibility to esca disease appears to vary between cultivars, under artificial or natural infection. However, the mechanisms and varietal characteristics underlying cultivar susceptibility to esca are still unknown.

L’unité de gestion technique d’un vignoble est aujourd’hui la parcelle. Néanmoins, au sein d’une même parcelle, la variabilité de l’expression végétative et de la constitution des raisins à maturité, peut être grande, en particulier à cause d’une hétérogénéité du sol.

Context and purpose of the study ‐ Recently early defoliation (ED) has been tested in several high‐ yielding grapevine varieties and sites aiming at reducing cluster compactness and hence, regulating yield and susceptibility to botrytis bunch rot infection. The reported results have been generally positive, encouraging growers to use this canopy management technique as an alternative for replacing the conventional time‐consuming cluster thinning and, simultaneously, as a sustainable practice to reduce the use of fungicides. However, ED increases berry sunburn risks and/or can induce carry‐over effects on vigor and node fruitfulness as shown in the two case studies reported in this work.