terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - New biotechnological tools 9 Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

Abstract

One of the main plant defence mechanisms is the Systemic Acquired Resistance (SAR) mediated by Salicylic Acid (SA). This is a heightened and broad-spectrum immune response initiated by the exposure to pathogens, inducing resistance not only in the infected site, but also throughout the entire plant. It was demonstrated that plant immune system can be regulated by two classes of SA receptors: NONEXPRESSOR OF PR GENES 1 (NPR1) and NPR1-LIKE PROTEIN 3 and 4 (NPR3/NPR4). While NPR1 is required for SA-induction followed by the expression of pathogenesis-related (PR) protein and resistance against pathogens, NPR3/NPR4 serve as transcriptional co-repressors of SA-responsive genes. The aim of this work was to trigger SAR by suppressing NPR3, and to investigate how plant response affects its ability to recruit beneficial microorganisms, specifically arbuscular mycorrhizal fungi (AMF). To this aim, embryogenic calli were obtained from anther and ovaries of grapevine (cultivar Chardonnay) and NPR3 knock out lines were achieved using CRISPR/Cas9 technique. Three regenerated lines, along with a backbone and a wild-type lines, were inoculated in axenic condition with the AMF Rhizophagus irregularis to test their recruitment ability. After the acclimatation, plants were transferred in the greenhouse and forty-five days later both roots and leaves were collected. Root colonization was evaluated using the Trouvelot method and significant differences in colonization level were observed among plants. Consequently, root DNA and RNA were extracted for metabarcoding and RNAseq analysis. Additionally, metabolomic analysis targeting metabolites involved in plant-microorganism interactions are ongoing.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Ivan Bevilacqua1,2, Loredana Moffa1, Serena Varotto2, Raffaella Balestrini3, Walter Chitarra1,3, Luca Nerva1,3

1Council for Agricultural Research and Economics – Research Centre for Viticulture and Enology (CREA-VE). Via XXVIII Aprile, 26, 31015 Conegliano (TV), Italy
2University of Padua, Department of Agronomy, Food, Natural Resources, Animals and Environment, Agripolis, Viale dell’Università 16 – 35020 Legnaro (Pd)
3National Research Council of Italy – Institute for Sustainable Plant Protection (IPSP-CNR). Strada delle Cacce, 73, 10135 Torino (TO), Italy 

Contact the author*

ivan.bevilacqua@crea.gov.it

Keywords

CRISPR/Cas9, grapevine, arbuscular mycorrhizal fungi (AMF), Systemic Acquired Resistance (SAR), multi-omics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of Ecklonia maxima seaweed extract on the vegetative, reproductive and microbiome in Vitis vinifera L. cv Cabernet-Sauvignon

Context and purpose of the study. Climate change is a major challenge in wine production. It results in erratic weather conditions which may lead to a reduction in grape yield and the subsequent grape and wine quality.

Read More

Deciphering the color of rosé wines using polyphenol targeted metabolomics

The color of rosés wines is extremely diverse and a key element in their marketing. It is due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making.

Read More

Terroir zoning in appellation campo de borja (northeast Spain): Preliminary results

The components and methodology for characterization of the terroir have been described by Gómez-Miguel & Sotés (1993-2014, 2003) and Gómez-Miguel (2011) taking into account the full range of environmental factors (i.e: climate, lithology, vegetation, topography, soils, altitude, etc.), landscape variables (derived from photo-interpretation and a digital elevation model), and specific variables to the country’s viticulture (i.e: size and distribution of the vineyards, varieties, phenology, productivity, quality, designation regulations, etc.).

Read More

Comparative QTL mapping of phenology traits in three cross populations of grapevine

Long-term studies on grapevine phenology have clearly demonstrated that global warming is affecting phenological events, leading to an anticipation in their timing, and negatively impacting grape yield and berry quality. Therefore, dissecting the genetic determinants involved in the plant regulation of the phenological stages of budburst, flowering, veraison and ripening can improve our knowledge of the underlying mechanisms and support plant breeding programs and the advancement of vineyard management strategies.
We report here the results of a QTL mapping experiment conducted on three segregating populations obtained from the crossing of ‘Cabernet Sauvignon’ and ‘Corvina’, ‘Corvina’ and the hybrid ‘Solaris’ and ‘Rhine Riesling’ and ‘Cabernet Sauvignon’.

Read More

Recovery of olfactory capacity following a COVID-19 infection

In this video recording of the IVES science meeting 2021, Sophie Tempère (Institut des Sciences de la Vigne et du Vin – ISVV, Université de Bordeaux) speaks about the recovery of olfactory capacity following a COVID-19 infection. This presentation is based on an original article accessible for free on IVES Technical Reviews.

Read More