Enhancing hydric stress tolerance by editing the VviMYB60 promoter with CRISPR/Cas9 

Terroir is a holistic concept that relates to both environmental and cultural factors that together influence the grape growing to wine production continuum. The physical factors that influence the process include matching a given grape variety to its ideal climate along with optimum site characteristics of elevation, slope, aspect, and soil

Prior to winemaking, organic or mineral nitrogen compound concentrations are usually measured in the vineyard and in grape musts. These indicators facilitate vine cultivation decisions, usually through yield or vigor. During vinification, yeast and bacteria metabolize nitrogen compounds in the musts in order to generate biomass. After fermentation, the microorganisms rerelease a part of this nitrogen as soluble compounds into the wines. Another part remains bound in the lees and can be lost during racking. The must’s natural nitrogen quantities, additional supplements during fermentation, and lees contact management enhance the release of nitrogen compounds to the wines. During ageing these nitrogen compounds – primarily the amino acids – are implicated in the generation of odorous compounds such as heterocycles(1).

Warming trends over the winegrowing regions lead to an advance of grapevine phenology, diminution of yield and increased sugar content and must pH with a lower polyphenol content, especially anthocyanins. Canopy management practices are applied to control the source sink balance and improve the cluster microclimate to enhance berry composition. We hyphothesized that an early leaf removal might promote a delayed ripening through severe defoliation after fruitset; whereas, a late leaf removal at mid-ripening would reduce sugar accumulation.

Over the past few decades, viticultural research has made numerous contributions which have made it possible to better understand the behavior of the vine as well as its response to the conditions imposed on it by the environment and agronomic practices. However, these results have only rarely been used in the practical management of vineyards because the research has been carried out using partial experimental models where reality is only represented by a few factors which are sometimes even made more complex by the introduction of elements foreign to the existing situation and difficult to apply to production (varieties, methods of cultivation, management techniques, etc.). To these reasons, one could add a low popularization of the results obtained, as well as the difficulty of implementing the scientific contributions, which does not allow the different production systems to fully express their potential. This limit of viticultural research can only be exceeded by the design of integrated projects designed directly on and for the territory. Indeed, only the integrated evaluation of a viticultural agro-system, which can be achieved through zoning, makes it possible to measure, or even attribute to each element of the system, the weight it exerts on the quality of the wine.

Le Muscat de Frontignan est bien connu pour ses fortes teneurs en composés terpéniques et par l’odeur florale et fruitée que ces composés confèrent aux vins qui en sont issus (1,2).