Enhancing hydric stress tolerance by editing the VviMYB60 promoter with CRISPR/Cas9 

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.

Life cycle assessment (LCA) is an effective and comprehensive method for evaluating the environmental impact of a product, considering its entire life cycle. In the context of wine production, although the use of lca is gaining ground in viticulture, its application is still limited to the fine assessment of winemaking processes.

Wine production is expanding in Scandinavia with a focus on organic growing, and Solaris becoming the signature grape of the region.

Pesticide residues in Austrian wines have so far been poorly documented. In 250 wines, 33 grape musts and 45 musts in fermentation, no limit values were exceeded, but in some cases high lev-els (>0.100 mg/l) of single residues were found, meaning that a reduction of these levels before bottling could make sense. In the course of this study, a white and a red wine were spiked with a mix of 23 pesticide residues from the group of fungicides (including botryticides), herbicides and insecticides. The influence of the following treatments on residue concentrations and volatile profiles were investigated: two activated charcoal products, a bentonite clay, two commer-cial mixed fining agents made of bentonite and charcoal, two yeast cell wall products, and a plant fiber-based novel filter additive. The results of this study show that all the agents tested reduced both residues and aromavolatile compounds in wine, with activated charcoal having the strongest effect and bentonite the weakest. The mixed agents and yeast wall products showed less aroma losses than charcoal products, but also lower residue reduction. Plant fibers showed good reduction of pesticides with moderate aroma damage, but these results need to be con-firmed under practical conditions.

A study of soil pH on the experimental field resulted in a high variability of pH on a very small scale. This kind of heterogenity in soil pH have effects on growth of two grapevine varieties on rootstock Kober 5BB