Enhancing hydric stress tolerance by editing the VviMYB60 promoter with CRISPR/Cas9 

Grapes (Vitis sp.) are considered a major source of phenolic compounds such as flavonols, anthocyanins and stilbenes. Studies related to the beneficial effects of these compounds on health have encouraged research aimed at increasing their concentration in fruits. On this behalf, several plant growth regulators such as jasmonic acid and its volatile ester, methyl-jasmonate (MeJa), have demonstrated promising results in many fruits. However, Brazilian subtropical climate might interfere on treatment response. The present study aims to evaluate the application of MeJa in the pre-harvest period in Concord and Isabel Precoce grapes (Vitis labrusca L.).

Condensed tannins (also called proanthocyanidins) are a widely distributed throughout in plants kingdom and are one of the most important classes of secondary metabolites, in addition, they are part of the human diet. In wine, they are extracted during the winemaking process from grape skins and seeds. These compounds play an important role in red wine organoleptic characteristics such as color, bitterness and astringency. Condensed tannins in red wine are oligomers and polymers of flavan-3-ols unit such as catechin, epicatechin, epigallocatechin and epicatechin-3-O-gallate. The monomeric units can be linked among them with direct interflavanoid linkage or mediated by aldehydes.

The increase in air temperature that is occurring in many important wine-growing areas around the world is resulting in the decoupling between the phenolic and the technological maturity of grapevine berries. This new ripening pattern leads to the production of light-bodied high alcoholic wines, but this is in countertendency with the increasing consumers’ demand for wines with low-to-mid alcohol concentrations. The oenological techniques proposed to reduce wine alcohol content are often very expensive and lead to detrimental effects on wine quality. Many viticultural practices have been proposed to slow down sugar accumulation the berry. One possible strategy that was previously found to be suitable for Aglianico grapevine is post-veraison shoot trimming. The aim of this work was to assess the carry over effects on the following year of shoot trimming and vine water status on yield and total soluble solids because the expected reduction in vine fertility could lead to a reduction in the effectiveness of shoot trimming.

The Pomerol vineyard, located 35 km east of Bordeaux, covers around 800 ha on the left bank of the Isle. There is a system of fluvial terraces with more or less coarse gravel and pebble spreading, resting on a Tertiary substratum ranging from the Middle to Upper Eocene to the Lower Oligocene (Dubreuilh, 1993). This interweaving of terraces of varying thickness results in a brutal superposition of differentiated materials which give rise to various types of soil. Several site studies in this sector of the Libounais show significant morphological and analytical differences from one point to another (Guilloux et al ., 1978; Duteau, 1982; Van Leeuwen et al.., 1989). The distribution of the soils of the Pomerol vineyard was studied and resulted in a cartography at 1/25000th (Merouge, 1995).

The study was carried out in 2006 with Merlot grapes from vines grown using the trellis system, where four treatments were compared with different levels of water stress.