Enhancing hydric stress tolerance by editing the VviMYB60 promoter with CRISPR/Cas9 

The detection of reducing compounds such as phenolic acids, anthocyanins or tannins is of prime importance to decipher on the antioxidant and anti-aging properties of wines.

Botrytis bunch rot occurrence is the most important limitation for the wine industry in humid environments. The effect of grapevine vegetative growth on bunch rot expression results from direct effects (cluster architecture, nitrogen status among others) and indirect ones (via microclimate). Previous studies of our group showed strong differences in bunch rot incidence between floor management treatments: cover crop (CC) vs weed-free strips under the trellis with herbicide (H). We observed that in some circumstances this reduction in bunch rot incidence occurred without major vine growth differences among treatments. The aim of the present study was to test the general hypothesis that other factors unrelated to grapevine vegetative expression could be more relevant to grapevine susceptibility to bunch rot.

The choice of sites for viticulture depends on natural environmental factors, particularly climate, as grapevines have specific climatic requirements for optimum physiological performance and berry quality achievement. In the Stellenbosch wine-producing region, the complex topography and the proximity of the ocean create a variety of topoclimates resulting in different growth conditions for vines within short distances.

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.

Nitrogen (N) fertilizer is important for grape growth and the quality of wine. It is essential to address the mismatch between N application and wine composition. Cabernet Gernischt (Vitis vinifera L.), as one of the main wine-grape cultivars in China, was introduced to Yantai wine region in 1892. This grape cultivar is traditionally used for quality dry red wine with fruit, spices aroma, ruby red and full-bodied wines. In order to regulate vine growth and improve grape and wine quality, Cabernet Gernischt grapevines were subjected to decreased levels of N treatments, compared to normal N supply treatment, during grape growing seasons of 2019 and 2020.