Enhancing hydric stress tolerance by editing the VviMYB60 promoter with CRISPR/Cas9 

In addition to studies on the edaphic and landscape characteristics of the environment (Dolédec, 1995), the characterization of the physiology of the vine and of parasitism during its vegetative cycle represents an essential component of knowledge and management of the terroirs.

Effective control with fungicides is essential to protect grapevine from downy mildew, a devastating disease caused by the oomycete Plasmopara viticola. Managing this disease faces challenges in maintaining fungicide efficacy as the number of modes of action decreases and the risk of fungicide resistance increases. Long-term measures should address strains resistant to multiple modes of action, that can be selected by the repeated use of single-site fungicides. For these reasons, a precision management of the disease, that considers the selection of the best fungicide schedule according to the sensitivity profile of the pathogen population, is needed.

The presence of undesirable compounds in wines, such as OTA, biogenic amines and pesticides residues, affects wine quality and can cause health problems for the consumer. The main tool that a winemaker has to reduce their content in the wine is fining. However, some of the fining agents commonly used in the winery can cause allergies or even increase the protein content in the wine, increasing the turbidity. To avoid these problems, the use of plant fibers may be an alternative, such as those from grape pomace[1] or other plant origins.

In viticulture sector to find new tools for pest management has become an urgent necessity. Hence, grapevines cultivation has high production rate demand and to meet the intensive market request, a massive use of pesticides is often required. In addition to the environmental problems associated with large use of chemicals, there is an increasing number of consumers which are asking for

The aim of this ongoing study is to evaluate the degree of adaptability of grapevine scion:rootstock combinations to different conditions of water constraint. Here we present results from the young vine development phase, using three scenarios of water constraint that were implemented from planting. The experimental vineyard was established in 2020 and the data presented will cover the 2021/2022 and 2022/2023 seasons. The experiment consisted of the cultivars Pinotage (PIN), Shiraz (SHI) and Cabernet Sauvignon (CAB), grafted on two rootstocks, Richter 110 (R110) and USVIT-8-7 (US87).