Development and application of CRISPR/Cas in grapevine

AIM: Vitis vinifera L. cv. Moscato includes different varieties, mainly white grapes with a medium-sized berry, spheroidal or slightly flattened in shape, yellow greenish color which becomes golden yellow or amber when exposed to the sun. Moscato varieties are mainly used for the production of sweet aromatic wines

Bobal is the second most widely grown Spanish red grape variety (54,165 has), mainly cultivated in the Valencian Community and especially, in Utiel-Requena region (about 67% of 34,000 has). In this study, agronomic and enological parameters were determined in 98 biotypes selected during 2018 and 2019 in more than 50 vineyards over 50 years-old in the Utiel-Requena region. Moreover, a multi-criteria approach considering temperature and rainfall (Fig. 1A), among other parameters, was made to establish three different zones within the region (Fig. 1B), where in the future the selected biotypes will evaluated. In fact, in 2020, 4 replicates and 12 vines per biotype were planted in an experimental vineyard to preserve this important intra-cultivar diversity.

La superficie vitata della provincia di Modena é per circa il 70% interessata dai Lambruschi, famiglia di vitigni tipica dei territori pianeggianti emiliani. Tra questi, i più rappresentativi sono il Lambrusco di Sorbara, il Lambrusco salamino e il Lambrusco grasparossa che, unico esempio, predilige gli ambienti collinari della provincia. Nel quinquennio 2001-2005 la Provincia di Modena ed il C.R.P.V. hanno coordinato la zonazione viticola di tutto il territorio dei Lambruschi modenesi, i cui risultati hanno consentito di individuare, in ciascuna zona D.O.C., alcune Terre in cui cias.

Climatic changes cause significant variations in the composition of grapes. for red grapes, a mismatch between phenolic and technological ripening is often observed. There is also often a marked increase in pH and a reduction in fixed acids, which affect the stability and evolution of the wine during ageing. These experiments will provide more information on the role of pH during the winemaking of red wines on the extraction and evolution of phenolic compounds.

Context and purpose of the study. Conventional viticulture relies heavily on synthetic inputs (fertilizers, pesticides), as well as mechanization to manage pests, weeds, and diseases and maximize yields.