terclim by ICS banner
IVES 9 IVES Conference Series 9 Development and application of CRISPR/Cas in grapevine

Development and application of CRISPR/Cas in grapevine

Abstract

The development and application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) technologies have revolutionized genome editing in plants due to its simplicity, high efficiency, and versatility. As an economically important fruit crop worldwide, grapevine genome editing using CRISPR/Cas technologies has also been reported these years.  Here we introduce the development briefly of the most popular CRISPR/Cas9 system and also the state-of-the-art CRISPR technologies developed so far. Moreover, we summarize CRISPR/Cas9-mediated applications for gene functional study and trait improvement in grapevine. Optimization of CRISPR/Cas9 system, as well as the other CRISPR/Cas systems including CRISPR/LbCas12a and base editor in grapevine genome editing, is also discussed. In addition, we discuss the challenges and future perspectives for precision genome editing in grapevine, expecting to present a roadmap for the future applications of CRISPR technology in this species.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Zhenchang Liang1,2,3*, Chong Ren1,2,3

1 State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
2 Beijing Key Laboratory of Grape Sciences and Enology, Beijing 100093, PR China
3 China National Botanical Garden, Beijing 100093, PR China

Contact the author*

Keywords

grapevine, genome editing, CRISPR/Cas, challenges, future perspectives

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Pedoclimatic comparison of three viticultural areas of Italy devoted to high-quality Aglianico and Cabernet Sauvignon production

Aim: The study aims to show how different pedo-climatic conditions (past, present, and future) in three Italian sites at different latitudes (from center to southern), affect the adaptation of two red grapevine cultivars: Aglianico and Cabernet Sauvignon.

Read More

Study of the effect of native vineyard bacteria on the expression of Plasmopara viticolaeffectors

Downy mildew, caused by the oomycete Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni, is one of the most destructive grapevine diseases mostly affecting Vitis vinifera L. and impacting on viticulture. The pathogen invasion can induce in grapevine multiple defense reactions, first PAMP-Triggered Immunity and secondly Effector-Triggered Immunity. Plasmopara viticola can overcome these defense mechanisms through the secretion of effectors, such as RxLR, into the plant cells, making it easier for the oomycete to infect grapevines. Currently, the use of chemical pesticides remains the most effective way to control the pathogen with severe negative side effects on the environment and animal health.

Read More

Uncovering the effectiveness of vineyard techniques used to delay ripening through meta-analysis

One of the most concerning trends associated with increasing heat and water stress is advanced ripening of grapes, which leads to harvesting fruit at higher sugar concentrations but lacking optimal phenolic (i.e. color and mouthfeel) and aromatic maturity. Mitigation techniques for this phenomenon have been studied for many years and practices to delay sugar accumulation have been identified, including antitranspirants, delayed pruning and late-source-limitation techniques. Evaluation of the efficacy of these vineyard practices has occurred across a wide range of environments, vintages, varieties and growing conditions. To assess the broader efficacy of these three vineyard practices, which are easy-to-implement and cost-effective, a meta-analytic approach was adopted using data retrieved from 43 original studies.

Read More

Influence of pedoclimatic factors during berry ripening in Burgundy

Berry composition at ripeness can be explained by many factors. This study was carried out from 2004 through 2011 in a 60 block network in the Yonne region, Burgundy.

Read More

Epigenetics: an innovative lever for grapevine breeding in times of climatic changes

In this video recording of the IVES science meeting 2025, Margot Berger (INRAE, UMR1287 EGFV, Institut des Sciences de la Vigne et du Vin, Villenave d’Ornon, France) speaks about epigenetics as an innovative lever for grapevine breeding in times of climatic changes. This presentation is based on an original article accessible for free on OENO One.

Read More