Development and application of CRISPR/Cas in grapevine

La D.O. Ribeira Sacra (Galicia, N.O. de España) se distribuye a lo largo de las riberas de los ríos Miño y Sil. Su característica mas destacada son las fuertes pendientes. Desde 1990 se estudia el estado

During the more than 190 years since the founding of the first ampelographic collection, the creation of a series of collections is attested on the territory of the Republic of Moldova, each operating in different historical periods and socio-economic conditions,

Wine flavour is the integration of distinct physiologically defined sensory systems that combine taste, aroma and trigeminal sensations, and it is a key determinant factor for the acceptance of wine by consumers. Volatile compounds, are important contributors to wine flavour, specially to aroma. These small and low-boiling point compounds are easily released into the air allowing to enter and move within the nasal or oral cavities where they can bind the olfactory receptors. Additionally, wine also contains aroma precursors, which are non-volatile compounds, but that can be broken down releasing volatile odorants. During wine tasting, all these chemicals (volatiles and non-volatiles) can be submitted to the action of salivary enzymes.

Ozone is a potent oxidizing compound that quickly decomposes into oxygen without residues. Previous works reported that ozone is not only a disinfectant that directly harms the pathogens of the vine but also activates systemic defense systems in the plant by activating oxidative stress. We assume these systemic defense mechanisms are essential to the vines’ resistance to downy and powdery mildew (Plasmopara viticola & Erysiphe necator, respectively). The goals of the research are to examine the effect of spraying with ozone water on the plant’s resistance against the mentioned pathogens as well as to characterize the metabolic profile of the plants treated with ozone as well as physiological characteristics in the vines such as the level of Photosynthesis and crop yield. Vines in the vineyard sprayed with ozone water at concentrations of 2 and 4 PPM weekly and biweekly, untreated control & conventional spray. Leaves were taken from vines 2,4,7,9 and 11 days after exposure to ozone and inoculated with the pathogens.

“Côtes du Rhône”, like many other controlled appellation wine, represents high stakes in the economical, social cultural and historical domains. The scenery formed by vineyards reveals these cultural values. It offers by a pleasant and appealing environment for the inhabitants and the tourists. It is also a powerful marketing tool for the winemakers.