Development and application of CRISPR/Cas in grapevine

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.

As Vitis vinifera varieties are susceptible to fungal diseases, numerous chemical treatments are generally required to ensure the quantity and quality of the harvest. However, in the context of sustainable viticulture, there are increasing societal request, political incitation, and winegrowers’ awareness to reduce the use of pesticides.

Gewürztraminer is a well-known wine famous for its aroma profile, which is characterized by rose petals, cloves, lychees, and other tropical fruit notes.

This work aimed at investigating red wine olfactory–oral cross-modal interactions, and at testing their impact on the correlations between sensory and chemical variables. Seventy-four Italian red whole wines (WWs) from 10 varieties, and corresponding deodorized wines (DWs), were evaluated by sensory descriptive assessment.

150 years ago grafting was identified as the most efficient method to combat phylloxera, this stimulated the active breeding of grapevine rootstocks and provided numerous rootstocks that are still in use today. Since then and over the 20th century, only a few new genotypes were been released.