Development and application of CRISPR/Cas in grapevine

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

The bioprotection of musts or grapes is a strategy for limiting sulfiting during winemaking and more specifically at pre-fermentative step. The most preconized yeasts in bioprotection mainly belong to Metschnikowia pulcherrima and Torulaspora delbrueckii species. While previous studies have demonstrated that bioprotectant non-Saccharomyces strains were able to protect musts and wines against microbial spoilage as well as sulfites, they cannot protect must against oxidation which appears to be the main limit of this practice.

Sulfites (SO2) are commonly used in the wine industry to preserve products during storage for antiseptic and antioxidant purposes (Oliveira et al., 2011).

Depuis la création des outils d’amélioration et de suivi de la qualité, le CREDO développe et réalise des zonages de potentialités viticoles.

Dans la Région de la Bairrada (Litoral-Centre du Portugal), on a étudié au 1999, l’influence des différents systèmes de conduite sur le cépage rouge “Baga”, le plus important de la Région.