terclim by ICS banner
IVES 9 IVES Conference Series 9 Development and application of CRISPR/Cas in grapevine

Development and application of CRISPR/Cas in grapevine

Abstract

The development and application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) technologies have revolutionized genome editing in plants due to its simplicity, high efficiency, and versatility. As an economically important fruit crop worldwide, grapevine genome editing using CRISPR/Cas technologies has also been reported these years.  Here we introduce the development briefly of the most popular CRISPR/Cas9 system and also the state-of-the-art CRISPR technologies developed so far. Moreover, we summarize CRISPR/Cas9-mediated applications for gene functional study and trait improvement in grapevine. Optimization of CRISPR/Cas9 system, as well as the other CRISPR/Cas systems including CRISPR/LbCas12a and base editor in grapevine genome editing, is also discussed. In addition, we discuss the challenges and future perspectives for precision genome editing in grapevine, expecting to present a roadmap for the future applications of CRISPR technology in this species.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Zhenchang Liang1,2,3*, Chong Ren1,2,3

1 State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
2 Beijing Key Laboratory of Grape Sciences and Enology, Beijing 100093, PR China
3 China National Botanical Garden, Beijing 100093, PR China

Contact the author*

Keywords

grapevine, genome editing, CRISPR/Cas, challenges, future perspectives

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Investigating the Ancient Egyptian wines: The wine jars database

In Ancient Egypt, wine was a luxury product consumed mainly by the upper classes and the royal family and offered to gods in daily religious rituals in the temples.
Since the Predynastic (4000-3100 BC) period, wine jars were placed in tombs as funerary offerings. From the Old Kingdom (2680-2160 BC) to the Greco-Roman (332 BC-395 AD) period, viticulture and winemaking scenes were depicted on the private tombs’ walls. During the New Kingdom (1539-1075 BC), wine jars were inscribed to indicate: vintage year, product, quality, provenance, property and winemaker’s name and title.

Use of fumaric acid to control pH and inhibit malolactic fermentation in wines

In this audio recording of the IVES science meeting 2022, Antonio Morata (Universidad Politécnica de Madrid, Madrid, Spain) speaks about the use of fumaric acid to control pH and inhibit malolactic fermentation in wines.

Influence of phenolic composition and antioxidant properties on the ageing potential of Syrah red wines measured by accelerated ageing tests.

Red wine ageing impacts its chemical and sensory characteristics such as colour, astringency and aromas evolution. Wine ageing involves many chemicals and physico-chemical reactions. Oxygen has an important role in these evolutions, especially during bottle ageing. It is known that wine composition and its antioxidant capacity are correlated to its ability to undergo with oxygen exposure [1]. A high oxygen exposure can affect wine quality by the formation of undesirable oxidative volatile compounds such as acetaldehyde [2]. Thus, ageing capacity is an important factor for wine quality and is related to extent of oxidation with ageing [3].

Remote sensing applications in viticulture: recent advances and new opportunities

Remote sensing applications in viticulture have been a research theme now for nearly two decades, becoming a valuable tool for vineyard management. Metrics produced using remotely sensed images of vineyards have yielded relationships with grape quality and yield that can help optimise vineyard performance

Developing and assessing different cordon establishment techniques for long-term vineyard management

Aim: The aim of this research is to quantify the impacts of different cordon establishment techniques on vine health and longevity. It is hypothesised that wrapping developing cordon arms tightly around the cordon wire will cause a constriction of the vascular system, becoming worse over time and disrupting the flow of water and nutrients.