Development and application of CRISPR/Cas in grapevine

Root phylloxera (Daktulosphaira vitifoliae) infestation poses a persistent threat to viticulture, yet its latent form (characterized by minimal above-ground symptoms) remains poorly understood, especially under drought stress exacerbated by climate change.

The increase in air temperature that is occurring in many important wine-growing areas around the world is resulting in the decoupling between the phenolic and the technological maturity of grapevine berries. This new ripening pattern leads to the production of light-bodied high alcoholic wines, but this is in countertendency with the increasing consumers’ demand for wines with low-to-mid alcohol concentrations. The oenological techniques proposed to reduce wine alcohol content are often very expensive and lead to detrimental effects on wine quality. Many viticultural practices have been proposed to slow down sugar accumulation the berry. One possible strategy that was previously found to be suitable for Aglianico grapevine is post-veraison shoot trimming. The aim of this work was to assess the carry over effects on the following year of shoot trimming and vine water status on yield and total soluble solids because the expected reduction in vine fertility could lead to a reduction in the effectiveness of shoot trimming.

The european union has estimated that energy consumption for wine production is about 1,750 million kwh per year, of which 500 million kwh is attributable to italy. In recent years, Italy has emerged as the world’s leading wine producer with about 50 million hectoliters per year. About 20 percent (9.8 million hectoliters) of Italian wine is marketed after refermentation according to the Charmat-Martinotti method.

Chardonnay is the world’s most planted white grape variety and has met a great commercial success for decades.

In recent years, resistant varieties have returned to the attention of the wine sector as a response to climate change and the reduction of pesticides in grapevine management, which is the main culprit of pesticide use in European agriculture. In this context, the production of sparkling wines could be strongly influenced due to its requirements for a particular balance between sugars and acidity, and the necessity of sound grapes to ensure wine quality. However, these parameters are not the only ones that define the suitability of a grape variety to produce sparkling wine.