Development and application of CRISPR/Cas in grapevine

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.

Plusieurs caves coopératives de l’AOC Côtes du Rhône se servent des informations du zonage pour la sélection des vendanges en fonction du terroir d’origine, afin d’élaborer des «cuvées terroir» et d’exploiter ainsi le potentiel qualitatif de leurs secteurs.

Il a été souvent démontré (Seguin, 1970), que les meilleurs terroirs sont ceux qui présentent pendant la période de maturation du raisin, une régulation et une limitation de l’alimentation hydrique de la vigne. Si on s’intéresse aux facteurs influençant ce régime hydrique, on constate le rôle prépondérant du taux d’éléments grossiers non poreux qui limitent la réserve utile du sol en diminuant le taux de terre fine. De plus, ces éléments grossiers jouent également un rôle au niveau du pédo-climat thermique car leur conductivité thermique et leur chaleur spécifique sont plus élevées que celles de la terre fine. Ainsi le sol se réchauffera et se refroidira plus rapidement (Saini et McLean, 1967), (Gras, 1994).

Terroir is a complex concept which associates soil, climate, grape variety and cultural practices that include the training system and oenological techniques. It is a type of social construction with man at its centre. The typicality of a wine is also a social construction which is the result of an agreement among specialists vis à vis a given quality of the wine whose references are the wine’s origins (e.g. terroir) and taste.

In the face of climate change, new grapevine varieties will have to show an adaptive phenotypic plasticity to maintain production with erratic water resources, and still ensure the quality of the final product. Their selection requires a better knowledge of the genetic basis of those traits and of the elementary processes involved in their variability. ‘PlastiVigne’, an emblematic project of the Vinid’Occ key challenge, funded by the Occitanie Region (France), tackles this issue with innovative genomic and physiological tools implemented on a unique panel of grape genetic resources representing the genetic diversity of Vitis vinifera. A graph-pangenome is developed from a representative set of high-quality genomes to study the extent and impact of structural genome variations and chromosomal rearrangements in the rapid adaptation capacity of grapevine.