Development and application of CRISPR/Cas in grapevine

Nitrogen fertilization is an important practice to guarantee vineyards sustainability and performance over years, while ensuring berry quality. However, achieving a precise nitrogen fertilization to meet specific objectives of production is difficult. There is a lack of knowledge on the impact of nitrogen fertilizers (soil/foliar; organic/mineral) and different levels of fertilization on the interactions between carbon and nitrogen cycles within the vine. Crop models may be useful in that purpose because they can provide new insights of the effects of fertilization in carbon and nitrogen storage. The objective of this study is to build a model to simulate grapevine carbon and nitrogen content in vines to evaluate the impact of different fertilization strategies in vine growth and yield.

Understanding the composition of wine and how it is influenced by climate or wine-making practices is a challenging issue. Two approaches are typically used to explore this issue. The first approach uses chemical
fingerprints, which require advanced tools such as high-resolution mass spectrometry and multidimensional chromatography. The second approach is the targeted method, which relies on the widely available 1-D GC/MS, but involves integrating the areas under a few peaks which ends up using only a small fraction of the chromatogram.

We propose here a brand new large routine microbiological analysis method intended for oenology, in flow cytometry, using high performance equipment and triple selective cell staining, activated by fluorescence. The results and practical applications of the method are presented: Brettanomyces (Dekkera) Monitoring, fermentations monitoring, bottling and enological practices monitoring.The method allow a complete new microbiological tool for wine industry.The method has been accredited ISO 17025 in our laboratories.

AIM: Cross-over applications are an emerging technological approach in food microbiology where a microorganism from one traditional specific fermentation process is used to improve quality and safety in another agri-food production/chain (Dank et al., 2021). A complex microbial diversity is found in association with fermentation in wine, including Saccharomyces, non-Saccharomyces and malolactic bacteria,  all microorganisms versatile in terms of enological utilisation (Tempère et al., 2018). Here, we propose a systematic literature review highlighting the existing trends and possible future applications related to cross-over exploitation of wine-related microbiota. 

Gene expression and phenotypic characteristics of plants grown in contrasting environments can be modulated through different epigenetic mechanism such as DNA methylation. This epigenetic mark may act in a dynamic way in response to different environmental conditions were high altitude vines are developed.