Development and application of CRISPR/Cas in grapevine

Since the emergence of phylloxera, grafting has been the most used propagation method in viticulture. Despite all the improvement measures implemented in the nurseries, it is frequent that graft success rates vary depending on the nursery process and scion/rootstock combinations. The reasons behind this unsatisfactory behaviour are still unknown and can be diverse, although carbohydrate reserves might be hypothesised to be crucial, since callus, root, and new tissue formation will be built based on them. In order to identify the effect of carbohydrates on grafting success, nine combinations were established based on the starch content in grapevine scionwoods (cv. Tempranillo clone VN69) and rootstocks cuttings (110 Richter clone 237) used for grafting: Low (L), Medium (M), High (H).

The designations of origin of the Loire Valley wine have been recognized according to customs and notoriety established over the centuries since the Middle Ages. There are four main production basins going up the Loire, from Nantes to the Sancerrois region: Nantes, Anjou-Saumur, Touraine and the vineyards of the Centre. In each of these basins, there is a wide range of appellations of origin which has been established according to a logic which may not seem obvious to the uninformed.

In the context of climate change and the need to reduce inputs, optimising photosynthesis and grapevine performance requires a better understanding of the interactions between water status, nitrogen availability, and source-sink relationships.

Balsamic vinegars of Modena (Italy), namely Aceto Balsamico di Modena PGI (AB PGI) and Aceto Balsamico Tradizionale di Modena PDO (ABT PDO) are among the most important geographical indication products for Italy. ABT PDO, despite its very limited production, is recognized as one of the most representative Italian artisan gastronomic products, and it is known and commercialized all around the world. The economic value of ABT PDO (“affinato” and “extra-vecchio” types, depending on the aging), prepared following a traditional way and aged for many years in a set of barrels (transferring a certain amount of vinegar from one cask to another in a decreasing “topping up” procedure) is great, when compared to AB industrially prepared with caramel. AB PGI is certainly the most widespread industrial-type vinegar in the world, deriving from low-temperature condensed grape must (or cooked must) mixed with wine vinegar, obtaining balsamic vinegars with a caramel-like taste. Depending on its economic value, ABT PDO is often object of fraud, requiring to fight counterfeit products and imitations.

Vintage is part of « terroir ». The aim of this work is to study, through vine and berry parameters, the effect of vintage on the three major red grape varieties in Côtes du Rhône : Grenache N, Syrah N and Mourvedre N. We first characterized vintages 1997 to 2003, highlighting similar features in grape development across the different cultivars since 2001 only.