terclim by ICS banner
IVES 9 IVES Conference Series 9 Development and application of CRISPR/Cas in grapevine

Development and application of CRISPR/Cas in grapevine

Abstract

The development and application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) technologies have revolutionized genome editing in plants due to its simplicity, high efficiency, and versatility. As an economically important fruit crop worldwide, grapevine genome editing using CRISPR/Cas technologies has also been reported these years.  Here we introduce the development briefly of the most popular CRISPR/Cas9 system and also the state-of-the-art CRISPR technologies developed so far. Moreover, we summarize CRISPR/Cas9-mediated applications for gene functional study and trait improvement in grapevine. Optimization of CRISPR/Cas9 system, as well as the other CRISPR/Cas systems including CRISPR/LbCas12a and base editor in grapevine genome editing, is also discussed. In addition, we discuss the challenges and future perspectives for precision genome editing in grapevine, expecting to present a roadmap for the future applications of CRISPR technology in this species.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Zhenchang Liang1,2,3*, Chong Ren1,2,3

1 State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
2 Beijing Key Laboratory of Grape Sciences and Enology, Beijing 100093, PR China
3 China National Botanical Garden, Beijing 100093, PR China

Contact the author*

Keywords

grapevine, genome editing, CRISPR/Cas, challenges, future perspectives

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The influence of site aspect and pruning types on Pinot Noir phenology and shoot growth

Aim: Managing the influence that terroir in vineyards has on vine development depends on improving our understanding the effect of the interaction of within-site variability, within-vine variability, and management practices (such as pruning types) on phenology and vine development. This study evaluates the consequence of site aspect

Effects of the synergy between T. delbrueckii and S. cerevisiae in the winemaking of traditional cultivars from southeastern Italy

The combination of Torulaspora delbrueckii and Saccharomyces cerevisiae in co-inoculation and sequential inoculation in winemaking was investigated as an innovative strategy to increase the aromatic profile of wines like Verdeca and Nero di Troia wines, two traditional varieties from south-eastern Italy (Apulia Region).

Definition and planning of viticultural landscapes case study in the “Côtes du Rhône Gardoises”

Les préoccupations actuelles autour des paysages viticoles vont au-delà des clichés promotionnels développés par les stratégies marketing. En effet, les paysages sont aujourd’hui au cœur d’une demande sociale croissante qui se traduit par différentes lois (la loi paysage de 1993, le paysage reconnu comme patrimoine commun de la nation par la loi n°95-101, la création du Conseil national du paysage par arrêté du 8/12/2000).

Effects of wine versus de-alcoholised wine on the microbiota-gut-brain axis in a tau-pathology murine model of Alzheimer’s disease

Alzheimer’s Disease (AD) is the most common disorder associated with cognitive impairment and the main cause of dementia globally. Multiple evidence in the last decade suggest that the gut microbiome plays an important role in the pathogenesis and progression of AD via the microbiota-gut-brain axis, a network wherein microbiome and the central nervous system crosstalk via endocrine, immune, neural, and microbial metabolites signalling pathways.

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification.