Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Oral tribology receives growing attention in the field of food sciences as it offers great opportunities to establish correlations between physical parameters, such as the coefficient of friction, and sensory perceptions in the human mouth.

The need to rationalize agricultural inputs has recently increased interest in assessing vineyard variability in order to implement variable rate input applications, so-called ‘precision viticulture’. In many viticultural areas globally, precision viticulture is already widely used such as for selective harvesting and variable rate application (VRA) of inputs such as irrigation and/or fertilizer. Robust VRA relies on having a geostatistically accurate map (of one or more vineyard attributes) requiring high sampling densities, which can be cost- and time-prohibitive to obtain. Previous work on spatial interpolation using kriging have upscaled ground-based measurements, but such upscaling strategies are applicable only when vineyard conditions are spatially continuous and satisfies the assumption of second-order stationary processes. Alternatively, mixed models that combine kriging and auxiliary information, such as the regression kriging (RK) method, are more instructive for spatial predictions. In order to improve prediction accuracies, it is therefore necessary to incorporate additional information to achieve accurate spatial patterns with low error.

Aim: This work studies how successive O2 saturations affects the color and hydroxycinnamic
acids concentration in the absence and presence of laccase from B. cinerea with the aim of better understanding the browning processes.

Materials and methods: Grapes of Muscat of Alexandria were harvested and pressed with a vertical press to extract 60% of their juice. Aliquots of 30 mL of this must were placed in 60 mL flasks equipped with a pill (PreSens Precision Sensing GmbH) for measuring oxygen by luminescence (Nomasense TM O2 Trace Oxygen Analyzer).

A foresight study was carried out by a group of experts from INRAE, universities, INAO and FranceAgriMer from 2014 as part of the multidisciplinary “laccave” project intended to anticipate climate change in the French wine industry. The initial objective was to initiate an interdisciplinary dialogue between researchers and to feed their questions in a more systemic way. The scenario development method made it possible to build possible futures for the wine sector in the face of climate change. It began by drafting four adaptation strategies, combining different possible intensities of innovation and relocation of the vineyard.

The strategy for sustainability in the wine sector of the EU refers to a set of practices and principles that aim to minimize the negative impact of wine production on the environment, social and economic sustainability. Sustainable wine production involves a range of practices that are designed to reduce waste, conserve resources, and promote the well-being of workers and communities.