Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Most grape scion cultivars grown around the world are derived from a single species, Vitis vinifera. Yet, the proportion of interspecific hybrids is increasing for a variety of reasons, including resistance to abiotic stresses such as low temperatures; societal, economic and environmental pressures to reduce pesticide usage; and to add a greater range of flavors to new table grape cultivars.

Consumers predominantly use visual, aromatic and texture cues as quality/preference indicators to describe olfactory sensations. In this study, the effect of micro-organism in wine production was investigated using analytical and sensory techniques to achieve relevant analytical characterisation. Selected anthocyanins, flavan-3-ols, flavonols and phenolic acids were quantified in Syrah wines using RP-HPLC-DAD. Standard oenological parameters were also measured. Syrah grape must was fermented with various combinations of Saccharomyces cerevisiae (S. cerevisiae) and non-Saccharomyces (Metschnikowia pulcherrima or Hanseniaspora uvarum) yeasts, which was followed by sequential inoculation of lactic acid bacteria (LAB) (Oenococcus oeni or Lactobacillus plantarum).

In 2001, the Hungarian Ministry of Agriculture and Rural Development designated the Institute of Geodesy, Cartography and Remote Sensing (FÖMI) to elaborate a Geographic Information System (GIS) supported Vineyard Register (VINGIS) in Hungary. The basis of this work was a qualification methodology (vineyard and wine cellar cadastre system) dating back to several decades, however, in the 1980s and 1990s the available geographical maps and information technology did not provide enough accuracy for an overall evaluation of viticultural areas. The reason for the VINGIS elaboration and development was an obligation resulting from the EU membership to ensure the agricultural subsidies for the wine–viticulture sector.

Una de las biotecnologías más potentes para disminuir el pH en vinos de zonas cálidas y en variedades de pH elevado es el uso de la levadura no-saccharomyces lachancea thermotolerans. Esta especie es capaz de formar ácido láctico a partir de azúcares, reduciendo al mismo tiempo ligeramente el grado alcohólico. Por lo tanto, mejora dos de los principales problemas de los vinos de regiones afectadas por el calentamiento global. El ácido láctico es un ácido orgánico con una buena integración sensorial en el sabor del vino, y también química y biológicamente estable durante el envejecimiento del vino.

Il mal dell’esca é una malattia della vite della quale sono state studiate sintomatologia, eziologia, patogenesi ed epidemiologia. Essendo una malattia che colpisce soprattutto la parte epigea delle piante, le caratteristiche dei suoli non sono mai state considerate fra le responsabili della sua insorgenza e diffusione.