Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Plant-pathogen interactions can trigger extensive epigenetic modifications in the host genome.

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Grape growers around the world are seeking to reduce their reliance on herbicides. However, traditional alternatives to chemical weed control do not always integrate seamlessly into established vineyard operations. Employing nonchemical weed management often requires trellis alterations, purchasing or hiring new equipment, and depending on region, may significantly increase tractor passes required to reach desired level of weed control. Critical thinking and thoughtful strategies are necessary to minimize expenditures and maintain quality during the transition away from herbicides. In this trial, irrigation was installed underground in an effort to minimize water loss due to evaporation, better direct the water to the vines, and reduce weed growth in the difficult to control undervine area.

The typicality of a wine, as well as its aromatic identity, are attributes that are highly sought after and requested by the current market. It is therefore of considerable technological interest to investigate the aromatic aspects of specific wines and to identify the odorous substances involved.In this thesis work, the characterization of the aromatic composition of Prosecco wines available on the market with a price range between 7 and 13 euros was carried out. These wines came from three different areas of origin such as Valdobbiadene, Asolo and Treviso.

To meet the demand of assessment tool of vine growers and their advisers we adapted to the vine production the INDIGO® method to developed initially for arable farming.