Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

The past three decades of terrestrial remote sensing research have delivered unprecedented insights into our fundamental ability to detect, quantify, and differentiate plant disease (Gold 2021). However, much of our fundamental knowledge in this domain has come from studies in non-agricultural systems and until recently, most agricultural studies, when extant, have focused on tree crops where canopy closure and large plot and plant size facilitate stress detection at low spatial resolution. Recent engineering innovations and advancements in constellation architecture design have refined the accuracy and scalability of airborne and spaceborne sensing platforms, enabling us to monitor diverse specialty crops, including grapevine, planted in smaller, spatially varied fields.

The physical factors that influence the grape ripening include elevation, slope, aspect, potential global radiation, sun hours and soil type of the vineyards.

Winegrowers must adapt more and more their viticultural practices in order to evolve toward a sustainable viticulture, to be competitive and to improve both the production methods and the quality and typicalness of wines. In this context, ‘Terroir’ studies in Loire Valley vineyards have allowed to build decision aid maps that can be used directly by growers to adjust their practices.

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Definitions of terroir elude consistent agreement. As defined geographical space the common denominators of its conceptualization include natural and cultural elements of life