Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Grapevine grafting is a complex process that since the establishment of phylloxera has become mandatory for grapevine. Grafting success in grapevine nurseries considerably varies among years and batches with most variety/rootstock combinations reach a high success rate (between 75% and 90%), but some combinations show lower success rates of around 40-50%. The causes of this variation are unknown, although biotic stresses like those caused by some viral infections have been demonstrated to affect the process. European certification schemes for the vegetative propagation of the vine include five major viruses (Arabis mosaic virus, Grapevine Fanleaf Virus, Grapevine Fleck Virus, and Grapevine-associated Leafroll Virus 1 and 3).

Consumers and the wine industry tend to agree on the ability of old vines to produce fruit that allows the production of wine of superior character. However, despite past and ongoing research, objective evidence of this point of view is still debated and studies on robust, specifically dedicated plots are scarce. Thus the impact of grapevine age on berry oenological potential and wine quality remains an open question. To try to objectively address the issue, a unique vineyard was established at Geisenheim University, Germany. It was planted in 1971 with cv. Riesling grafted on 5C Teleki. In 1995 and 2012, several rows were uprooted and replanted with the same rootstock/scion combination, resulting in a vineyard with alternate rows of identical plant material, but with different planting dates. The parameters of technical maturity and grape composition at harvest were analyzed during seasons 2014, 2015, 2016 and 2017 combining HPLC and enzymatic methods. Separate micro-vinifications were made for each age group and wine composition was analyzed by a combination of 1H-NMR and SPE-GC-MS.

There are claims that wine grape cultivars are either isohydric or anisohydric; the former maintaining, and the latter decreasing, their plant water status as soil moisture declines. However, available information is inconsistent. There are those that show an existence of a continuum in cultivar response to soil moisture rather than a distinct categorization. Others even show both behaviors in the same cultivar grown in different environments. In this study we investigated the behavior of 30 own rooted Vitis vinifera cultivars during successive drydown and rewatering cycles over two growing seasons in arid eastern Washington (<200 mm annual precipitation).

The introduction of ai-driven tools in digital content creation represents a significant shift in the landscape of marketing, particularly for industries reliant on rich visual storytelling such as the wine sector. The development of ai models like openai’s sora, runway’s gen-2 or google’s lumiere, which can generate realistic video content from textual descriptions, offers promising new avenues for enhancing brand narrative and consumer engagement. This research explores the potential of text-to-video (t2v) ai models to revolutionize wine marketing by creating dynamic, engaging content that captures the essence of vineyards and their products without the need for traditional video production processes.

The timing of phenology is critical if grape quality potential is to be optimized. Phenological process based models are used to predict phenology. In this study, three different models