Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Today the knowledge in terms of molecular composition of the colloidal matrix is ​​not enough in order to control its stability, according to the number of winemaking and wine stabilization processes. The physico-chemical processes during the winemaking change the composition and quantity of wine macromolecules. The goal today is to determine which analytical techniques will allow to discriminate these winemaking processes in order to better understand their impact on colloidal matrix stability as well as which molecules are responsible for its instabilities. METHODS: Wines obtained after conventional winemaking were subjected to different fining and chemical stabilization treatments. Different methods were used to investigate the wine macromolecular composition and stability after chemical stabilization, including quantitative and qualitative analyzes of total soluble polysaccharides by extraction under acidified ethanol, and by size exclusion separation as well as qNMR metabolomics. RESULTS: Observation of a slight difference at the quantitative level using classical analysis between the winemaking processes was observed as well as a strong discrimination by qNMR metabolomics.

Une étude préliminaire des situations météorologiques journalières a été réalisée pour l’Afrique du Sud et pour les mois de février (période de maturation des raisins dans la Province occidentale du Cap), à l’image de la classification synoptique réalisée aux latitudes tempérées en France (Jones & Davis, 2000), afin d’étudier les relations entre le climat et la viticulture à des latitudes plus basses.

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.

Grape composition is strongly influenced by climate conditions. Their expected modifications in near future, notably because of increased temperatures, could significantly modify the biochemical composition of berries at harvest, and thus wine typicity and quality. Elevated temperatures favor sugar accumulation in grapes, enhance malic acid degradation and modify the amino acid content. They also reduce significantly anthocyanin accumulation in Merlot, leading to the imbalance between anthocyanins and sugars, while no significant effects on final anthocyanin levels were reported in Tempranillo[1] and finally affect aromas or aroma precursors.

L’étude présentée porte sur une commune de l’Entre-Deux-Mers dans le bordelais. Nous caractérisons dans un premier temps les potentialités des sols vis-à-vis de la production de vins rouges de qualité (délimitation de terroirs grâce à la prise en compte des couvertures pédologiques et des caractéristiques morphométriques du terrain : pentes, expositions, convexités. Dans un second temps, nous récapitulons l’évolution historique des occupations des sols depuis 1790 : l’emplacement des vignes est ainsi localisé sur les terroirs respectifs des communes. Enfin, une étude prospective résultant d’enquêtes sur l’utilisation du foncier, situe le devenir prévisible de l’espace étudié (en particulier dans ses composantes viticoles et urbaines).