Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.

Bottle ageing is a critical period for wine quality, as it undergoes various chemical and sensory changes during storage. Ideally, a phase of qualitative ageing, during which wine sensory quality improves, is followed by a decline of quality. Understanding how different oenological variables influence these phases is a key challenge in modern winemaking. Recent studies highlighted the significant role of oxygen in modulating reactions involving volatile and non-volatile components, impacting aroma evolution during bottle aging. Oxygen exposure of wine during bottle ageing is mediated by closure.

‘Pinot Precoce Noir’ (PPN) is an early ripening clone of ‘Pinot Noir’ (PN). The phenological differentiation is visible by an about two weeks earlier onset of veraison. It was found that the early veraison locus Ver1 on chromosome 16, previously identified in ‘Calardis Musqué’, originated from PPN. A highly correlated SSR marker, namely GF16-Ver1, was developed and tested for its ability to molecularly differentiate between PPN and PN as well as its potential to trace individual descendants.

Mediterranean vineyards have been traditionally grown under rainfed conditions, but in recent decades the irrigated area has increased significantly, seeking to minimize the adverse effects of severe water stress on grape quality and yield. Given the large area occupied by vineyards, and the increasing scarcity of water resources, it is necessary to develop strategies for the optimization and efficient use of water to reduce the risk of its overexploitation. The present study aims at valorizing previous knowledge generated in different research projects by means of a meta-analysis of the effects of water status management on vineyard performance.

The species and origin used for red wine oak aging determines the physiological composition of the wood and thus the finished wines. In America, oak is grown primarily