Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Usually the winemaker consider polyphenols from the grape berry as an actor of the wine quality. There are frequently consider as a marker of grape maturity. It is commonly known that winemaker consider tannins and anthocyanins as main polyphenol actors for winemaking practices and wine quality. Here we will focus on the characterisation of lignins in grape seeds. Previous studies suggest that the seed is lignified [1], which could explain the change in colour of the seed when it reaches maturity and thus provide a reliable indicator for describing the maturity stage in the seed.

The precise molecular mechanisms underlying the domestication of grapevine (Vitis vinifera L.) Are still not fully understood. In the recent years, next-generation sequencing (NGS) of plastid genomes has emerged as a powerful and increasingly effective tool for plant phylogenetics and evolution. To uncover the biological profile of the grapevine domestication process comprehensively, an investigation should encompass both the cultivated varieties (V. vinifera subsp. Vinifera) and their wild ancestors V. vinifera subsp. Sylvestris) across all potential sites of their distribution and domestication.

Winemaking generates a significant amount of waste. Grape marc, the main solid residue, constitutes 20-25% of the pressed grapes and approximately 8-9 million tons are produced globally each year.

Un 94 % del viñedo español se cultiva con métodos y técnicas propias de los sistemas agrícolas desarrollados en secano en regiones de clima semiárido, donde las precipitaciones anuales raramente exceden los 500 mm

The effectiveness of enzyme-mediated maceration processes in red winemaking relies on a clear picture of the target (berry cell wall structure) to achieve the optimum combination of specific enzymes to be used. However, we lack the information on both essential factors of the reaction (i.e. specific activities in commercial enzyme preparation and the cell wall structure of berry tissue). In this study, the different combinations of pure recombinant enzymes and the recently validated high throughput cell wall profiling tools were applied to extend our knowledge on the grape berry cell wall polymeric deconstruction during the winemaking following a combinatorial enzyme treatment design.