Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Aim: Climate change is altering some aspects of winegrape production with an advancement of phenological stages which may endanger viticultural areas in the event of a late frost. This study aims to evaluate the potential of satellite-based remote sensing to assess the damage and the recovery time after late frost events.

The shelf life of wine is a major concern for the wine industry. This is particularly true for wines intended for long cellaring, which are supposed to reach their peak after an ageing period ranging from a few months to several years, or even decades. Low, controlled oxygen inputs through the closure system are generally necessary for the wine to evolve towards its optimum organoleptic characteristics. Our previous studies have already shown that the interface between the cork and the bottleneck plays a crucial role in the transfer of oxygen into the bottled wine.

Pour prévoir et gérer chaque année les principales caractéristiques de la maturation en Champagne, le CIVC (Comité Interprofessionnel du Vin de Champagne) a développé un ensemble de moyens de prévision et d’information très performants qui permettent aux différents acteurs de la filière viti-vinicole de prendre en compte ces informations à l’échelle de chaque terroir communal pour la recherche d’une qualité optimale.

For coopers, toasting process is considered a crucial step in barrel production during which oak wood (Q. petraea) develops several aromatic nuances released to the wine during its maturation. Toasting consists of applying different degrees of heat to a barrel for a specific period. As the temperature increases, thermal degradation of oak wood structure produces a huge range of chemical compounds. Many studies have identified the main key aroma volatile compounds (whisky-lactone, furfural, eugenol, guaiacol, vanillin). However, detailed information on how the chemical structure of oak wood degrades with increasing toasting level is still lacking.

The dynamic evolution of some bioclimatic indices largely used to define the vocation of areas to grape growing was assessed over 43 years (1965-2007) in four sites of the Abruzzo Region (Central Italy).