terclim by ICS banner
IVES 9 IVES Conference Series 9 A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

Abstract

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes. In parallel, we were able to assess the efficiencies of several guide RNAs (gRNAs) targeting genes with fall-out on drought stress and pathogen resilience. To the first aim, a gene belonging to glutathione S-transferase (VvGST40) and two gene belonging to pectin-methyl esterase (VvPME1 and VvPME3) have been targeted. Several edited lines were acclimatized and are currently under evaluation. In parallel, two genes belonging to the Mildew Locus-O (VvMLO6 and VvMLO7) and a non-expressor pathogenesis related gene (VvNPR3) were targeted to improve pathogen resilience. Acclimatized plants edited for MLO genes resulted almost resistant to Erysiphe necator (disease incidence reduction up to 80%), whereas NPR3-edited vines showed a significant reduction in disease severity (up to 70%). In conclusion, our approaches allowed to improve stress resilience of several economically-important genotypes such as Pinot noir, Chardonnay, Sangiovese and Glera but also widely used rootstocks (e.g., 110R and K5BB).

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1*, Loredana Moffa1, Anna Narduzzo1, Ivan Bevilacqua1, Irene Perrone2, Chiara Pagliarani2, Giorgio Gambino2, Claudio Lovisolo3, Riccardo Velasco1, Walter Chitarra1

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)
2 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

powdery mildew, climate change, recalcitrance, cisgenic-like approach, New Plant Breeding Techniques

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Vine response to compost addition on a sandy-loam soil in the north-east of italy. Effects on root system, vegetative growth, yield and grape quality of Cabernet-Sauvignon cv

In this study two different compost types and two application methods were studied over 5 years (2009-2013) on mature Cabernet Sauvignon vines grown in a commercial vineyard in the AOC Piave area, northeastern Italy.

Effect of rootstock and preplant fumigation on plant parasitic nematode development in Washington wine grapes

In Washington State, the majority of winegrape (Vitis vinifera) vineyards are planted to their own roots. This practice is possible due to the lack of established phylloxera populations, and is preferred due to the ease of retraining after damaging winter cold events. However, own-rooted V. vinifera is generally susceptible to most plant parasitic nematodes that attack grape. In Washington State, management of nematodes is dominated by preplant soil fumigation. One practice that may mitigate economic loss due to nematodes is the adoption of nematode-“resistant” rootstocks.

Smart microgrid: how to reduce costs and CO2 emissions in wineries and vineyards

The wine sector is greatly threatened by climate change, but is also one of its contributors.

Teasing apart terroir: the influence of management style on native yeast communities within Oregon wineries and vineyards

Newer sequencing technologies have allowed for the addition of microbes to the story of terroir. The same environmental factors that influence the phenotypic expression of a crop also shape the composition of the microbial communities found on that crop. For fermented goods, such as wine, that microbial community ultimately influences the organoleptic properties of the final product that is delivered to customers. Recent studies have begun to study the biogeography of wine-associated microbes within different growing regions, finding that communities are distinct across landscapes. Despite this new knowledge, there are still many questions about what factors drive these differences. Our goal was to quantify differences in yeast communities due to management style between seven pairs of conventional and biodynamic vineyards (14 in total) throughout Oregon, USA. We wanted to answer the following questions: 1) are yeast communities distinct between biodynamic vineyards and conventional vineyards? 2) are these differences consistent across a large geographic region? 3) can differences in yeast communities be tied to differences in metabolite profiles of the bottled wine? To collect our data we took soil, bark, leaf, and grape samples from within each vineyard from five different vines of pinot noir. We also collected must and a 10º brix sample from each winery. Using these samples, we performed 18S amplicon sequencing to identify the yeast present. We then used metabolomics to characterize the organoleptic compounds present in the bottled wine from the blocks the year that we sampled. We are actively in the process of analysing our data from this study.

Natural variability and vine-growers behaviour

Le vigneron est confronté à une variabilité naturelle omniprésente, liée au millésime et aux facteurs pédoclimatiques. Depuis 10 ans, en Champagne, la relation qu’entretient le vigneron avec l’espace a évolué. Les exemples d’entreprises collectives à vocation territoriale se sont multipliés : gestion de l’hydraulique viticole, maillages de groupements de conseil viticole (Magister), sites en confusion sexuelle, réseau maturation, analyses de sols par secteur, …