A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

Viticulture worldwide is currently affected by the effects of climate change. This set of adverse phenomena lead to a deterioration of functional vine mechanisms, affecting growth, physiology and grape ripening, which may cause severe losses with respect to yield and quality. To prevent water stress and other abiotic factors from severely affecting its physiology, the vine’s response is to reduce transpiration and photosynthesis rates. This response varies depending on the cultivar and its ability to adapt to the environment. The hydroscape method is based on the internal regulation of water status in the plant. It has been recently used to classify grapevine genotypes according to their iso/anisohydric behavior when they are subjected to water stress conditions.

The study of cultural practices to delay ripening and the characterization of their effect on wine composition is important in the mitigation of accelerated ripening caused by higher temperatures

The global consumer demand for low or non-alcoholic wine is growing steadily in recent years, driven by health concerns, religious beliefs, and personal taste preferences etc.. Consequently, the removal of alcohol from wine can significantly alter its chemical and sensory properties, including color, aroma, and taste, which make a significant challenge for consumer to accept these products. Ethanol plays a crucial role in various chemical reactions and interactions that contribute to the development of wine’s characteristics.

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role. Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application. We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.

The reaction between Strecker amino acids and α-dicarbonyls is a key pathway in the formation of Strecker aldehydes (SA), which are crucial oxidation-related odorants in wine [1].