terclim by ICS banner
IVES 9 IVES Conference Series 9 A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

Abstract

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes. In parallel, we were able to assess the efficiencies of several guide RNAs (gRNAs) targeting genes with fall-out on drought stress and pathogen resilience. To the first aim, a gene belonging to glutathione S-transferase (VvGST40) and two gene belonging to pectin-methyl esterase (VvPME1 and VvPME3) have been targeted. Several edited lines were acclimatized and are currently under evaluation. In parallel, two genes belonging to the Mildew Locus-O (VvMLO6 and VvMLO7) and a non-expressor pathogenesis related gene (VvNPR3) were targeted to improve pathogen resilience. Acclimatized plants edited for MLO genes resulted almost resistant to Erysiphe necator (disease incidence reduction up to 80%), whereas NPR3-edited vines showed a significant reduction in disease severity (up to 70%). In conclusion, our approaches allowed to improve stress resilience of several economically-important genotypes such as Pinot noir, Chardonnay, Sangiovese and Glera but also widely used rootstocks (e.g., 110R and K5BB).

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1*, Loredana Moffa1, Anna Narduzzo1, Ivan Bevilacqua1, Irene Perrone2, Chiara Pagliarani2, Giorgio Gambino2, Claudio Lovisolo3, Riccardo Velasco1, Walter Chitarra1

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)
2 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

powdery mildew, climate change, recalcitrance, cisgenic-like approach, New Plant Breeding Techniques

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

White wine lees: unlocking the relationship between chemical composition and antioxidant potential

The wine-making process generates numerous by-products at each stage (crushing, fermentation, ageing), including wine lees, which account for almost 25% of the total quantity.

METHYL SALICYLATE: A TRENDY COMPOUND MARKER OF ZELEN, A UNIQUE SLOVENIAN VARIETY

The wine market interest for autochthonous varieties, particularly from less known wine regions, has significantly raised in the past few years. In that context, Slovenia, a small country from central Europe with a long winemaking tradition, is getting more and more attention, particularly through its range of unique regional varieties. Among them, Zelen, meaning “green” in Slovene, can only be found in the Vipava valley region, located on the western side of the country, near the border with Italy. When they are young, Zelen wines display very singular aromas reminiscent of rosemary, sage and white fruit. Despite its uniqueness, Zelen wine aromatic typicality is poorly documented in the literature.

HOW OXYGEN CONSUMPTION INFLUENCES RED WINES VOLTAMMETRIC PROFILE

Phenolic compounds play a central role in sensory characteristics of wine, such as colour, mouthfeel, flavour and determine its shelf life. Furthermore, the major non-enzymatic wine oxidation process is due to the catalytic oxidation of phenols in quinones. Due their importance, during the years have been developed different analytical methods to monitor the concentration of phenols in wine, such as Folin-Ciocalteu method, spectrophotometric techniques and HPLC. These methods can also be used to follow some oxidation-related chemical transformations.

INVESTIGATION INTO MOUSY OFF-FLAVOR IN WINE USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY WITH STIR BAR SORPTIVE EXTRACTION

Mousy off-flavor is one of the defects of microbial origin in wine. It is described as a particularly unpleasant defect reminiscent of rodent urine (a “dirty mouse cage”), and grilled foods such as popcorn, rice, crackers, and bread crust. Prior to the 2010s, mousiness was very uncommon but it has been becoming more frequent in recent years. It is often associated with an increase in pH as well as certain oenological practices, which tend to significantly decrease the use of sulfur dioxide.

Différenciation de parcelles de Chenin du Val de Loire, a l’aide de l’etude des flores fongiques des raisins, en utilisant l’outil DGGE

Depuis le millésime 2002, une étude est menée sur la diversité de la flore fongique de parcelles du cépage chenin, situées essentiellement sur les appellations de Vouvray et Montlouis ; deux appellations séparées par le fleuve nommé la Loire. Les parcelles se situent dans des conditions pédoclimatiques différentes, qui se retrouvent au travers des suivis de maturité et l’état sanitaire.