A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

After some decades sunk into oblivion, pruning has recently recovered the focus of grape growers and viticulturists worldwide. Attention is now being paid to the respect the sap flow continuity and to pruning wounds, as they may affect the general performance and longevity of the plant. The longevity and profitability are strongly affected by the increasing incidence of grapevine wood diseases (GWD), intensified by the omission of good pruning practices and leading to an increasingly aggressive pruning. The purpose of this study is to provide an objective evaluation of the short- and mid-term implications of different pruning practices that differ in the degree of observation several of pruning principles.

The available plant diversity is maintained in global genetic collections and germplasm banks. One of the main objectives of the study of the genetic material of vine still conducting research to characterize the genotypes and the creation of new varieties. The main ampelographic collection of the country, the largest in the Balkans, is located at the Athens Vine Institute in Lykovrisi, Attica, in an area of 70 acres. It contains more than 800 varieties, most of which are indigenous. The Institute is conducting research on the genetic improvement of native varieties and the creation new winemaking and table grape varieties of high productivity, grape quality, resistance to fungal diseases and their adaptability to stresses using the hybridization method using European high-quality varieties.

The importance of polyphenols, which are present in many vegetables and grapes too, is well-know and documented. Specific research works about the red grape

Grapes from warm(ing) climates often contain excessive sugars but lack acidity. This can lead to highly alcoholic wines with compromised stability and balance. The yeast Lachancea thermotolerans can ameliorate such wines due to its metabolic peculiarity – partial fermentation of sugars to lactic acid. This study aimed to elucidate the population-wide diversity in L. thermotolerans, whilst selecting superior strains for wine sector. An extensive collection of isolates (~200) sourced from different habitats worldwide was first genotyped on 14 microsatellite loci. This revealed differentiation of L. thermotolerans genetic groups based on the isolation substrate and geography. The 94 genotyped strains were then characterised in Vitis vinifera cv. Chardonnay fermentations.

Nell’ambiente del Chianti Classico è stato applicato un progetto di zonazione aziendale con l’objettivo di valorizzare le produzioni dei diversi vigneti. In particolare sono stati individuati sette siti, sottoposti a studio particolareggiato per un triennio.