terclim by ICS banner
IVES 9 IVES Conference Series 9 A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

Abstract

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes. In parallel, we were able to assess the efficiencies of several guide RNAs (gRNAs) targeting genes with fall-out on drought stress and pathogen resilience. To the first aim, a gene belonging to glutathione S-transferase (VvGST40) and two gene belonging to pectin-methyl esterase (VvPME1 and VvPME3) have been targeted. Several edited lines were acclimatized and are currently under evaluation. In parallel, two genes belonging to the Mildew Locus-O (VvMLO6 and VvMLO7) and a non-expressor pathogenesis related gene (VvNPR3) were targeted to improve pathogen resilience. Acclimatized plants edited for MLO genes resulted almost resistant to Erysiphe necator (disease incidence reduction up to 80%), whereas NPR3-edited vines showed a significant reduction in disease severity (up to 70%). In conclusion, our approaches allowed to improve stress resilience of several economically-important genotypes such as Pinot noir, Chardonnay, Sangiovese and Glera but also widely used rootstocks (e.g., 110R and K5BB).

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luca Nerva1*, Loredana Moffa1, Anna Narduzzo1, Ivan Bevilacqua1, Irene Perrone2, Chiara Pagliarani2, Giorgio Gambino2, Claudio Lovisolo3, Riccardo Velasco1, Walter Chitarra1

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)
2 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
3 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy

Contact the author*

Keywords

powdery mildew, climate change, recalcitrance, cisgenic-like approach, New Plant Breeding Techniques

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Selected ion flow tube mass spectrometry: a promising technology for the high throughput phenotyping of grape berry volatilome

Wine grapes breeding has been concentrating a lot of efforts within the grape research community over the last decade. The quick phenotyping of genotype quality traits including aroma composition remains challenging. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS), a technology first available in 2008 and developing rapidly, could be particularly valuable for this usage. The aims of this study were i) to use SIFT-MS, to analyze the whole volatilome from different grape varieties, ii) to assess the ability of this technology to discriminate varieties according to their grape aroma composition, and iii) to study the stability of SIFT-MS signal over maturation to define a sampling strategy.

Read More

Cluster trait prediction using hyperspectral signatures in a population of 221 Riesling clones

Cluster architecture in grapevine plays a critical role in influencing bunch microclimate, thus quality traits, including sugar content, phenolic composition, and disease susceptibility.

Read More

Impact of the non-volatile matrix composition on red wine aroma release and perception of olfactory and oral cues

Aroma and mouthfeel cues are the main characteristics defining red wine quality. During wine tasting, perceptual and physical-chemical phenomena leading to mutual interactions between volatiles and non-volatiles sensory active compounds, can occur. Aroma perception depends on the release of volatiles from wine, that is affected by wine constituents present in the medium (Pittari et al. 2021; Lyu et al. 2021). Our aim was to evaluate the effect of the non-volatile wine matrix composition (polyphenols, PPh) on the release and perception of red wine aromas by an experiment of matrix enrichment.

Read More

VOLATILE COMPOSITION OF WINES USING A GC/TOFMS: HS-SPME VS MICRO LLE AS SAMPLE PREPARATION METHODOLOGY

Wine aroma analysis can be done by sensorial or instrumental analysis, the latter involving several me-thodologies based on olfactometric detection, electronic noses or gas chromatography. Gas Chromatography has been widely used for the study of the volatile composition of wines and depending on the detection system coupled to the chromatographic system, quantification and identification of individual compounds can be achieved.

Read More

Comparison of destructive and non-destructive measurements of table grape berries to assess quality parameters using spectroscopy

The quality of table grapes is critically influenced by several parameters, including sugar content, acidity, firmness, and overall appearance.

Read More