A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

Context and purpose of the study. The breeding of PIWI varieties has a long tradition in the Czech Republic. In the last two years, 9 new PIWI varieties have been registered.

Consumers and the wine industry tend to agree on the ability of old vines to produce fruit that allows the production of wine of superior character. However, despite past and ongoing research, objective evidence of this point of view is still debated and studies on robust, specifically dedicated plots are scarce. Thus the impact of grapevine age on berry oenological potential and wine quality remains an open question. To try to objectively address the issue, a unique vineyard was established at Geisenheim University, Germany. It was planted in 1971 with cv. Riesling grafted on 5C Teleki. In 1995 and 2012, several rows were uprooted and replanted with the same rootstock/scion combination, resulting in a vineyard with alternate rows of identical plant material, but with different planting dates. The parameters of technical maturity and grape composition at harvest were analyzed during seasons 2014, 2015, 2016 and 2017 combining HPLC and enzymatic methods. Separate micro-vinifications were made for each age group and wine composition was analyzed by a combination of 1H-NMR and SPE-GC-MS.

AIM: Agiorgitiko (Vitis vinifera L. cv.) is the most cultivated red grapewine variety in Greece1 located mainly in Nemea region, the largest PDO zone in Greece2. Although Agiorgitiko is considered as one of the most interesting red grape varieties, not only in Greece3, but also at international level4,5, however, there is a lack of knowledge

Commercial red wines use the malolactic fermentation process to ensure stability from a microbiological point of view. In this second fermentation, malic acid is converted into L-lactic acid under controlled steps.

Water is the main limiting factor for yield in viticulture. Improving drought adaptation in viticulture will be an increasingly important issue under climate change. Genetic variability of water deficit responses in grapevine partly results from the rootstocks, making them an attractive and relevant mean to achieve adaptation without changing the scion genotype. The objective of this work was to characterize the rootstock effect on the diurnal regulation of scion transpiration. A large panel of 55 commercial genotypes were grafted onto Cabernet Sauvignon. Three biological repetitions per genotype were analyzed. Potted plants were phenotyped on a greenhouse balance platform capable of assessing real-time water use and maintaining a targeted water deficit intensity. After a 10 days well-watered baseline period, an increasing water deficit was applied for 10 days, followed by a stable water deficit stress for 7 days. Pruning weight, root and aerial dry weight and transpiration were recorded and the experiment was repeated during two years. Transpiration efficiency (ratio between aerial biomass and transpiration) was calculated and δ13C was measured in leaves for the baseline and stable water deficit periods. A large genetic variability was observed within the panel. The rootstock had a significant impact on nocturnal transpiration which was also strongly and positively correlated with maximum daytime transpiration. The correlations with growth and water use efficiency related traits will be discussed. Transpiration data were also related with VPD and soil water content demonstrating the influence of environmental conditions on transpiration. These results highlighted the role of the rootstock in modulating water deficit responses and give insights for rootstock breeding programs aimed at identifying drought tolerant rootstocks. It was also helpful to better define the mechanisms on which the drought tolerance in grapevine rootstocks is based on.