terclim by ICS banner
IVES 9 IVES Conference Series 9 Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Abstract

New Plant Breeding Techniques (NPBTs) have the potential to revolutionize the genetic improvement of grapevine. However, the practical application of these techniques is limited by several challenges, such as the difficulty in generating embryogenic calluses, maintaining their competence during in vitro cultivation, and regenerating plants without defects. To overcome these challenges, we conducted a study to test the effect of two treatments on callus cultures derived from different grapevine varieties, with and without embryogenic competence. The tested substances were Silver Thiosulphate (STS) an ethylene inhibitor, and Salicylic Acid (SA), an elicitor with different effects depending on the concentration of use beyond the ethylene inhibitor activity. Our observations revealed a differential response to the treatments depending on the tested variety. In some varieties, STS treatment enhanced the embryogenic competence of the calluses, while also having a growth-depressing effect on the non-embryogenic calluses. In contrast, the effect of SA was more dose-dependent and varied across different varieties. In some cases, the highest tested concentration of SA had a growth-depressing effect on both embryogenic and non-embryogenic calluses, while in other cases, it only affected embryogenic calluses. Despite these variations, both STS and SA treatments showed promising results in enhancing embryogenic competence, and we are currently evaluating the regeneration of embryos from callus after these treatments and the combined effects of STS and SA. Our study highlights the importance of testing the efficacy of different treatments on multiple grapevine varieties to identify the most effective strategies for NPBT applications.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Lucia Rosaria Forleo1*, Bruna Suriano1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Annalisa Prencipe2, Teodora Basile1, Riccardo Velasco1, Maria Francesca Cardone1, Carlo Bergamini1

1 Council for Agricultural Research and Economics -Research Center Viticulture and Enology (CREA-VE), Via Casamassima 148-70010 Turi (Ba), Italy
2 Department of Biosciences, Biotechnology and Environment, University of Bari “Aldo Moro”, 70125 Bari, Italy

Contact the author*

Keywords

embryogenic callus, ethylene inhibitor, silver thiosulphate, salicylic acid

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The interplay between grape ripening and weather anomalies – A modeling exercise

Current climate change is increasing inter- and intra-annual variability in atmospheric conditions leading to grapevine phenological shifts as well altered grape ripening and composition at ripeness. This study aims to (i) detect weather anomalies within a long-term time series, (ii) model grape ripening revealing altered traits in time to target specific ripeness thresholds for four Vitis vinifera cultivars, and (iii) establish empirical relationships between ripening and weather anomalies with forecasting purposes. The Day of the Year (DOY) to reach specific grape ripeness targets was determined from time series of sugar concentrations, total acidity and pH collected from a private company in the period 2009-2021 in North-Eastern Italy. Non-linear models for the DOY to reach the specified ripeness thresholds were assessed for model efficiency (EF) and error of prediction (RMSE) in four grapevine cultivars (Merlot, Cabernet Sauvignon, Glera and Garganega). For each vintage and cultivar, advances or delays in DOY to target specified ripeness thresholds were assessed with respect to the average ripening dynamics. Long-term meteorological series monitored at ground weather station by means of hourly air temperature and rainfall data were analyzed. Climate statistics were obtained and for each time period (month, bimester, quarter and year) weather anomalies were identified. A linear regression analysis was performed to assess a possible correlation that may exist between ripening and weather anomalies. For each cultivar, ripeness advances or delays expressed in number of days to target the specific ripening threshold were assessed in relation to registered weather anomalies and the specific reference time period in the vintage. Precipitation of the warmest month and spring quarter are key to understanding the effect of climate change on sugar ripeness. Minimum temperatures of May-June bimester and maximum temperatures of spring quarter best correlate with altered total acidity evolution and pH increment during the ripening process, respectively.

On quality assurance of winemaking components

This report examines product quality assurance issues arising when technological aids and food additives are utilized in winemaking.

Méthodologie pour application et valorisation des études de terroir dans les caves cooperatives des Côtes du Rhône (France)

L’appellation d’origine contrôlée “Côtes du Rhône” se caractérise par une très forte implantation du mouvement coopératif. Afin de mieux exploiter le potentiel qualitatif de leurs terroirs, plusieurs coopératives élaborent des “cuvées terroir”, résultat des sélections de vendanges provenant de différents secteurs.

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.

Temperature variations in the Walla Walla valley American Viticultural Area

Variations in average growing season and ripening season temperatures within the Walla Walla Valley American Viticultural Area are related to elevation and regional and local topography.