terclim by ICS banner
IVES 9 IVES Conference Series 9 Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Abstract

New Plant Breeding Techniques (NPBTs) have the potential to revolutionize the genetic improvement of grapevine. However, the practical application of these techniques is limited by several challenges, such as the difficulty in generating embryogenic calluses, maintaining their competence during in vitro cultivation, and regenerating plants without defects. To overcome these challenges, we conducted a study to test the effect of two treatments on callus cultures derived from different grapevine varieties, with and without embryogenic competence. The tested substances were Silver Thiosulphate (STS) an ethylene inhibitor, and Salicylic Acid (SA), an elicitor with different effects depending on the concentration of use beyond the ethylene inhibitor activity. Our observations revealed a differential response to the treatments depending on the tested variety. In some varieties, STS treatment enhanced the embryogenic competence of the calluses, while also having a growth-depressing effect on the non-embryogenic calluses. In contrast, the effect of SA was more dose-dependent and varied across different varieties. In some cases, the highest tested concentration of SA had a growth-depressing effect on both embryogenic and non-embryogenic calluses, while in other cases, it only affected embryogenic calluses. Despite these variations, both STS and SA treatments showed promising results in enhancing embryogenic competence, and we are currently evaluating the regeneration of embryos from callus after these treatments and the combined effects of STS and SA. Our study highlights the importance of testing the efficacy of different treatments on multiple grapevine varieties to identify the most effective strategies for NPBT applications.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Lucia Rosaria Forleo1*, Bruna Suriano1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Annalisa Prencipe2, Teodora Basile1, Riccardo Velasco1, Maria Francesca Cardone1, Carlo Bergamini1

1 Council for Agricultural Research and Economics -Research Center Viticulture and Enology (CREA-VE), Via Casamassima 148-70010 Turi (Ba), Italy
2 Department of Biosciences, Biotechnology and Environment, University of Bari “Aldo Moro”, 70125 Bari, Italy

Contact the author*

Keywords

embryogenic callus, ethylene inhibitor, silver thiosulphate, salicylic acid

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Cordon height and deficit irrigation practices interact to affect yield and fruit quality of Cabernet Sauvignon and petite Sirah grown in a hot climate

Cabernet Sauvignon and Petite Sirah are the top red wine cultivars in CA, however, the hot climate in Fresno is not ideal for red Vitis Vinifera, particularly for berry color development. Mechanical pruning and irrigation were studied previously to significantly affect grapevine yield performance and berry quality. But there is lack of studies on cordon height and irrigation on mechanical pruned vineyard system.

The role of the landscape as a component of the terroir in Spain (DO Somontano, NE Spain)

The components and methodology for characterization of the terroir in Spain have been described by Gómez-Miguel et al. (2003), Sotés et al. (2003), taking into account the full range of environmental factors (i.e: climate, vegetation, topography, soils, altitude, etc.),

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.

Chemistry and analysis of key volatile compounds of wine and their precursors in grape

A relatively small number of the many volatile substances of wine, often present at trace
concentrations, are considered as key volatile compounds. These compounds often exist in grapes
under poorly odoriferous or non volatile forms as aroma precursors.

The effect of wine matrix on the initial release of volatile compounds and their evolution in the headspace

There is evidence in the literature that non-volatile wine matrix can modify the release and therefore the perception of the compounds involved in wine aroma [1-3].