terclim by ICS banner
IVES 9 IVES Conference Series 9 Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Abstract

New Plant Breeding Techniques (NPBTs) have the potential to revolutionize the genetic improvement of grapevine. However, the practical application of these techniques is limited by several challenges, such as the difficulty in generating embryogenic calluses, maintaining their competence during in vitro cultivation, and regenerating plants without defects. To overcome these challenges, we conducted a study to test the effect of two treatments on callus cultures derived from different grapevine varieties, with and without embryogenic competence. The tested substances were Silver Thiosulphate (STS) an ethylene inhibitor, and Salicylic Acid (SA), an elicitor with different effects depending on the concentration of use beyond the ethylene inhibitor activity. Our observations revealed a differential response to the treatments depending on the tested variety. In some varieties, STS treatment enhanced the embryogenic competence of the calluses, while also having a growth-depressing effect on the non-embryogenic calluses. In contrast, the effect of SA was more dose-dependent and varied across different varieties. In some cases, the highest tested concentration of SA had a growth-depressing effect on both embryogenic and non-embryogenic calluses, while in other cases, it only affected embryogenic calluses. Despite these variations, both STS and SA treatments showed promising results in enhancing embryogenic competence, and we are currently evaluating the regeneration of embryos from callus after these treatments and the combined effects of STS and SA. Our study highlights the importance of testing the efficacy of different treatments on multiple grapevine varieties to identify the most effective strategies for NPBT applications.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Lucia Rosaria Forleo1*, Bruna Suriano1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Annalisa Prencipe2, Teodora Basile1, Riccardo Velasco1, Maria Francesca Cardone1, Carlo Bergamini1

1 Council for Agricultural Research and Economics -Research Center Viticulture and Enology (CREA-VE), Via Casamassima 148-70010 Turi (Ba), Italy
2 Department of Biosciences, Biotechnology and Environment, University of Bari “Aldo Moro”, 70125 Bari, Italy

Contact the author*

Keywords

embryogenic callus, ethylene inhibitor, silver thiosulphate, salicylic acid

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A new graphical interface as a tool to integrate data from GC-MS and UPLC-MS-QTOF: new compounds related with port wine aging

Port wine value is related to its molecular profile resulting from the changes occurring during the ageing period. It is of empirical knowledge that the style is greatly affected by the oxidation regimens, i.e. bottle versus barrel storage

Does Dekkera/Brettanomyces wine spoilage raise the risk of biogenic amines intake? A screening in Portuguese red wines

Wine quality and safety are the main concerns of consumers and health agencies. Biogenic amines and polyamines, depending on their concentration and on individuals, in wine can constitute a potential public health concern due to their physiological and toxicological effects

Ripening of Vitis vinifera grapes varieties in São Joaquim, a new wine growing region, Southern Brazil

This report has investigated the ripening characteristics of Vitis vinifera grapes Cabernet Franc, Merlot, Sangiovese and Syrah in two consecutive vintages (2006 and 2007), in order to evaluate the adaptation from these recently varieties planted in São Joaquim town, Santa Catarina State, Brazil.

Climate projections over France wine-growing region and its potential impact on phenology

Climate change represents a major challenge for the French wine industry. Climatic conditions in French vineyards have already changed and will continue to evolve. One of the notable effects on grapevine is the advancing growing season. The aim of this study is to characterise the evolution of agroclimatic indicators (Huglin index, number of hot days, mean temperature, cumulative rainfall and number of rainy days during the growing season) at French wine-growing regions scale between 1980 and 2019 using gridded data (8 km resolution, SAFRAN) and for the middle of the 21th century (2046-2065) with 21 GCMs statistically debiased and downscaled at 8 km. A set of three phenological models were used to simulate the budburst (BRIN, Smoothed-Utah), flowering, veraison and theoretical maturity (GFV and GSR) stages for two grape varieties (Chardonnay and Cabernet-Sauvignon) over the whole period studied. All the French wine-growing regions show an increase in both temperatures during the growing season and Huglin index. This increase is accompanied by an advance in the simulated flowering (+3 to +9 days), veraison (+6 to +13 days) and theoretical maturity (+6 to +16 days) stages, which are more noticeable in the north-eastern part of France. The climate projections unanimously show, for all the GCMs considered, a clear increase in the Huglin index (+662 to 771 °C.days compared to the 1980-1999 period) and in the number of hot days (+5.6 to 22.6 days) in all the wine regions studied. Regarding rainfall, the expected evolution remains very uncertain due to the heterogeneity of the climates simulated by the 21 models. Only 4 regions out of 21 have a significant decrease in the number of rainy days during the growing season. The two budburst models show a strong divergence in the evolution of this stage with an average difference of 18 days between the two models on all grapevine regions. The theoretical maturity is the most impacted stage with a potential advance between 40 and 23 days according to wine-growing regions.

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.