Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed.

This essay was aimed to describe the clonal diversity of Oenococcus oeni in the malolactic fermentation of the carbonic maceration (CM) winemaking. The free and the pressed liquids from CM were sampled and compared to the wine from a standard winemaking with previous destemming and crushing (DC) of grapes [1]. O. oeni strain typification was performed by PFGE as González-Arenzana et al. described (2014) [2]. Results showed that 13 genotypes, referred as to letters, were distinguished from the 49 isolated strains, meaning the genotype “a” the 27%, the “b” the 14%, the “c” the 12%, the “d and e” the 10 % each other, and the remaining ones less than the 8% each one.

Bud death due to cold damage is a recurrent and major economic issue with Vitis vinifera L. in the Northeastern U.S. winegrowing regions. Primary buds – and sometimes secondary and tertiary buds – are often damaged by fluctuating temperatures in the winter and early spring. To maintain balanced vegetative and reproductive growth of a vine, pruning practices need to be adjusted to account for bud damage. Conventional bud damage assessment requires growers to sample canes/spurs, cut nodes with a razor blade, and then visually assess bud damage. This process is laborious and becomes a major barrier for damage-compensated pruning decision-making, leading to too few live buds per vine and the associated excessive vigor and low yield that result. The overarching goal of this study was to develop an active thermographic system for non-destructive detection of bud damage in the vineyard.

Un programme de recherche concernant les facteurs naturels et humains des terroirs viticoles a été développé dans le vignoble A.O.C. de l’Anjou, sur une surface d’environ 30.000 Ha

Obtaining high-quality RNA from grape tissues, including berry pulp, berry skins, stems, rachis, or roots, is challenging due to their composition, which includes polysaccharides, phenolic compounds, sugars, and organic acids that can negatively affect RNA extraction. For instance, polyphenols and other secondary metabolites can bind to RNA, making it difficult to extract a pure sample. Additionally, RNA can co-precipitate with polysaccharides, leading to lower extraction yield. Also, sugars and organic acids can interfere with the pH and ionic properties of the extraction buffer. To address these challenges, we optimized a protocol for RNA isolation from grape tissues.