terclim by ICS banner
IVES 9 IVES Conference Series 9 Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Effects of Silver Thiosulphate and Salicylic Acid on the long-term maintenance of the embryogenic callus of Vitis vinifera

Abstract

New Plant Breeding Techniques (NPBTs) have the potential to revolutionize the genetic improvement of grapevine. However, the practical application of these techniques is limited by several challenges, such as the difficulty in generating embryogenic calluses, maintaining their competence during in vitro cultivation, and regenerating plants without defects. To overcome these challenges, we conducted a study to test the effect of two treatments on callus cultures derived from different grapevine varieties, with and without embryogenic competence. The tested substances were Silver Thiosulphate (STS) an ethylene inhibitor, and Salicylic Acid (SA), an elicitor with different effects depending on the concentration of use beyond the ethylene inhibitor activity. Our observations revealed a differential response to the treatments depending on the tested variety. In some varieties, STS treatment enhanced the embryogenic competence of the calluses, while also having a growth-depressing effect on the non-embryogenic calluses. In contrast, the effect of SA was more dose-dependent and varied across different varieties. In some cases, the highest tested concentration of SA had a growth-depressing effect on both embryogenic and non-embryogenic calluses, while in other cases, it only affected embryogenic calluses. Despite these variations, both STS and SA treatments showed promising results in enhancing embryogenic competence, and we are currently evaluating the regeneration of embryos from callus after these treatments and the combined effects of STS and SA. Our study highlights the importance of testing the efficacy of different treatments on multiple grapevine varieties to identify the most effective strategies for NPBT applications.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Lucia Rosaria Forleo1*, Bruna Suriano1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Annalisa Prencipe2, Teodora Basile1, Riccardo Velasco1, Maria Francesca Cardone1, Carlo Bergamini1

1 Council for Agricultural Research and Economics -Research Center Viticulture and Enology (CREA-VE), Via Casamassima 148-70010 Turi (Ba), Italy
2 Department of Biosciences, Biotechnology and Environment, University of Bari “Aldo Moro”, 70125 Bari, Italy

Contact the author*

Keywords

embryogenic callus, ethylene inhibitor, silver thiosulphate, salicylic acid

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

Investigating kokumi flavour oligopeptides in wine

Kokumi is a complex sensation perceived as enhanced palatability. Under the influence of kokumi substances, foods/beverages tastes become more flavorful with increased intensity, spread, continuity, richness, harmony, and punch which are the six related characteristics corresponding to the Kokumi sensory concept [1].

Ripening behaviour and grape must quality of eleven white resistant varieties in Trentino

In a situation of uncertainty towards the overall effect of climate change and the reduction of pestice utilization on quality, the wine sector needs to maintain the profitability of producers, which inexorably depends on ensuring the quality of grapes and wines. Among the various alternatives that can be adopted, hybrid varieties carrying resistance genes are currently gaining the attention of researchers and producers. Some of them are already a reality and are included in the national catalogue of some countries, selected by research institutes all over Europe.

Study on the impact of clone on the varietal aroma of Xinomavro

It is well documented that varietal aroma is an important parameter of wine quality. Chemical compounds responsible for wine varietal aroma are sourced from secondary grape metabolites. Until today little research is conducted on the influence of vine clone on the grape aromatic content of Greek grape varieties. Xinomavro (Vitis vinifera L.) is one of the most important Greek grape varieties, valuable for the wine industry of Northern Greece since it contributes to the production of PDO wine of Naoussa, Amindeo and Goumenissa.

Are all red wines equals regarding their vulnerability to Brettanomyces bruxellensis ?

Odours deemed harmful by the consumer and described as “stable”, “horse sweat” or “burnt plastic” can be found in wines. The responsible molecules are volatile phenols, produced by a spoilage yeast: brettanomyces bruxellensis. This species is particularly well adapted to the wine environment and can resists many stresses such as a high alcohol level, a low ph or high levels of SO2, more or less efficiently depending on the strain considered.