Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

During alcoholic fermentation, acetaldehyde is the carbonyl compound quantitatively the most produced by yeasts after ethanol. The dynamics of acetaldehyde production can be divided into 3 phases. Early formation of this compound is observed during the lag phase at the beginning of fermentation before any detectable growth [1].

Research into the effects of the Terroir is of major interest for the wine sector. The study of Terroir-Vine-Grape relations, even if it is complex, is fundamental for all viticulture: indeed, the quality of the grape must be the result of the most reasoned agro-viticultural management of the vine possible, which must first, to respect a production balance. The goal sought by the winegrower is to obtain a wine, the optimized result of the interactions Terroir-Grape variety. This link to the terroir is therefore essential to establish by taking into account on the one hand the behavior of the vine (which is the cause), and on the other hand, its effects on the grapes and finally on the wine.

In winemaking, the use of wood products alternative to barrels, has become a useful tool for the achievement of numerous oenological objectives, including the fast release of desirable volatile and polyphenolic compounds, colour stabilization, and important economic advantages if compared to the traditional barrel production. Among a huge array of variables, the wood format, the vinification protocol, especially the moment of the infusion of the woods and the exposed surface area of the alternative woods are of relevant significance, since they may influence the speed and intensity of the aroma transfer from the wood to the wine defining different sensory profiles.

Low temperatures are required for the acquisition and maintenance of bud cold tolerance, which are necessary for grapevines to survive freezing temperatures in winter.

Climate change is having an unprecedented impact on the wine industry, which is one of the major agricultural sectors around the world. Global warming, combined with the variation in rainfall patterns and the increase in frequency of extreme weather events, is significantly influencing vine physiology and exposing, more frequently, plants to severe biotic and abiotic stresses. This represents a challenge for viticulturists who need to take complex decisions to adjust vineyard management and achieve oenological goals.