Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Context and Purpose of the Study. In many countries, the preservation of grape varieties with heritage value is ensured by genebanks of outstanding significance, which allow for the study of these varieties and the assessment of their future roles in response to environmental, market, and social challenges.

During bottle aging, the development of wine aroma through low and gradual oxygen exposure is often positive in red wines, but can be unfavorable in many cases, resulting in a rapid loss of fresh, fruity flavors. Prematurely aged wines are marked by intense prune and fig aromatic nuances that dominate the desirable bouquet achieved through aging (Pons et al., 2013). This aromatic defect, in part, is caused by the presence of 3-methyl-2,4-nonanedione (MND). MND content was shown to be lower in nonoxidized red wines and higher in oxidized red wines, which systematically exceeds the odor detection threshold (62 ng/L).

The State Rio Grande do Sul is the main producer of Brazilian fine wines, with four viticultural regions. The objective is the characterization of the viticultural climatic potential of the State (total surface of 281.749 km2). The methodology use the Géoviticulture Multicriteria Climatic Classification System (Géoviticulture MCC System), based on three climatic indices – Dryness Index (DI), Heliotermal Index (HI) and Cool Night Index (CI).

Bioprotection, leveraging beneficial microorganisms, has emerged as a sustainable approach to modern winemaking, minimizing reliance on chemical preservatives like as sulfur dioxide (SO₂).

Plastic film covering is a technique largely used in viticulture to protect table grapes vines from adverse weather conditions and to reduce the negative effects of grapevine fungi disease. Plastic film composition affects solar radiation income inside the covering with effects on sunlight wavelengths in relation to different absorbance and reflectance. The interaction of selected light ranges with vines could influence grape ripening and yield and consequently influence shelf life.