Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

During wine alcoholic fermentation, yeasts produce volatile aroma compounds from sugar and nitrogen metabolism. Some of the metabolic pathways leading to these compounds have been known for more than a century.

The marked climate change impact on vine and grape development (phenology, sugar content, acidity …) is one of the manifestations of Genotype X Environment X Management interactions importance in viticulture. Some practices, such as irrigation, can mitigate the effect of water deficit on grape development, but warming is much more difficult to challenge. High temperatures tend to alter the acid balance of the fruit with a parallel increase in sugar concentration. In the long term, genetic improvement to select varieties better coping with temperature elevation appear as a good option to support sustainable viticulture. Nevertheless, the existing phenotypic diversity for grape quality components that are influenced by temperature is poorly understood, which jeopardizes breeding strategies.

Tempranillo is the most widely grown red grapevine variety in Spain, currently representing 42% of the total number of red varieties and 21% of the total vineyard area. Due to the economic importance that this variety represents in Spanish viticulture, in some areas where it is traditionally grown, there is a special concern about the viability of the future growing of this variety is being compromised by the climate change effects.

The effects of (A) grape freezing, and (B) malolactic fermentation, have been evaluated on the chemical and colorimetric profiles of red wines from Schiava grossa cv. grapes, thus prone to colour instability. The aim was to observe if specific variables (e.g. grape freezing) could improve the extraction and stability of pigments. The samples were studied from musts up to twelve months in bottle. The study was conducted with independent parallel micro-vinifications (12 = 4 theses x 3 replicates) under strictly-controlled conditions.

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.