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IVES 9 IVES Conference Series 9 Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Abstract

Grapevine is a plant that holds significant socioeconomic importance due to its production of grapes for fresh consumption, wines, and juices. However, climate changes and susceptibility to diseases pose a threat to the quality and yield of these products. The breeding of new genotypes that are resistant/tolerant to biotic and abiotic stresses is essential to overcome the impact of climate changes. In this regard, Marker-assisted selection (MAS), which uses DNA markers, is a crucial tool in breeding programs. The efficiency and economy of this method depend on finding rapid DNA isolation methods. In this study, we compared four different DNA extraction methods to choose the one that quickly isolates DNA from many young vine leaves samples in a single run. The methods used involved Lithium chloride, carboxyl coated magnetic beads, cetyltrimethylammonium bromide (CTAB), and a commercial kit called Red&extract. The results showed that the CTAB method was the best in terms of reliability of the procedure, yield of the extracted DNA, low quantity of inhibitors, and speed of the procedure. Improving the MAS technique will help identify plants containing genes involved in different types of stress and deepen the study of the resistance genes pyramided.

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DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marika Santamaria1,2, Antonella Salerno1,2, Flavia Angela Maria Maggiolini2, Margherita D’Amico2, Carlo Bergamini2, Maria Francesca Cardone2

1 Department of Biosciences, Biotechnology and Environment, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, Italy, 2 Council for Agricultural Research and Economics -Research Center Viticulture and Enology (CREA-VE), Via Casamassima 148-70010 Turi (Ba), Italy

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Keywords

Vitis vinifera, Marker Assisted Selection, DNA isolation, breeding

Tags

IVES Conference Series | Open GPB | Open GPB 2024

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