Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Driven by the demand for sustainable agriculture, biocontrol is emerging as a crucial alternative to chemical fungicides for crop protection.

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

The combined effect of soil moisture and soil temperature on grapevine physiology is gaining interest in the context of global warming.

Les enzymes d’oxydation (polyphénoloxydase, peroxydase) des raisins sont d’origine génétique dépendantes des facteurs climatiques et agrotechniques (Sapis et al, 1983). Dans le processus technologique de l’obtention du moût de raisins, ces enzymes catalysent l’oxydation de certains composés phénoliques naturellement présents dans le raisin, produisant ainsi des modifications indésirables de la couleur et de l’arôme du vin.

One of the effects of warming trends is the advance of budburst, increasing the frequency of spring frost-related damage. In April 2021, a severe frost event affected central and northern italian viticulture. In a cv. Barbera vineyard located in the Colli Piacentini wine district, after such occurrence, vines were tracked and growth of primary bud shoots (PBS), secondary bud shoots (SBS), and suckers (SK) was monitored, as well as their fruitfulness and fruit composition. Vine performances were then compared to those of the previous year, when no post-budburst freezing temperatures occurred. The goal of the study was to evaluate the efficacy of SBS in restoring yield loss due to PBS injuries and analyze respective contribution to fruit composition.