Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

The historical Tokaj region in northeast Hungary is a UNESCO World Heritage region since 2002 owning 5.500 ha vineyards. Produced from „noble rot” grapes, Tokaji Aszú is known as one of the oldest botrytized wines all over the world. Special microclimatic conditions (due to Bodrog and Tisza rivers, Indian summer), soil circumstances (clay, loess on volcanic bedrock) and grape-varieties (Furmint, Hárslevelű) of Tokaj-region offer favourable parameters to the formation of noble rot caused by Botrytis cinerea. The special metabolic activity of Botrytis results in noble rot grapes called “aszú” berries. The grapes undergo complex chemical modifications as the joint result of the enzymatic activity of Botrytis and the physical process of concentration.

To characterize in depth the enological productions according to the origin territories and to provide modern tools for the qualitative raising of the assorted typologies of wine produced, Veneto Agricoltura (the regional agency for the agriculture, forestry and food industry development), the Regional Government of Veneto (north-eastern Italy) and various Consortia of Producers have undertaken since 2002 a systematic classification of the viticultural territories by agro-ecological zoning to achieve a strategic project aimed to set Veneto as the first Italian region to have completed in a systematic and scientifically rigorous way the zoning of most of its Denomination of Origin areas.

Due to altered climatic conditions, grape berry sunburn has become one of the main challenges in contemporary viticulture.

The author presents the geographical position of Romania as a vine-growing European country and analyses its relief and climate as factors of paramount importance for vine-growing environments. The climatogram system and the oenoclimatic aptitude index are applied in an analysis of the climatic characteristics of the Romanian vine-growing reg ions.

Grapevine Red Blotch disease (RB) is a recently discovered disease that has become a major concern for the viticulture and winemaking industry in California, USA. The causal
agent, Grapevine Red Blotch Virus (GRBV) was identified in 2011 and its presence was confirmed in several states in the US, in Canada, and in Switzerland. It has been demonstrated that RB compromised the regulation of ripening by suppressing specific ripening events, altering the expression patterns of transcription factors and causing hormonal imbalances in Zinfandel.