Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Trunk diseases and esca in particular, represent a major threat to the sustainability of the vineyards. The percentages of unproductive vines in a plot could vary from 4% to over 20 % depending on local conditions and vintages.

Temperature has a decisive influence on the growth and development of plants (Carbonneau et al., 1992). In particular, in the case of the vine, the temperature is an omnipresent variable in the climatic indices (Huglin, 1986). For reasons of convenience, these indices use the temperature of the air measured under shelter in a meteorological station, making the implicit hypothesis of a concordance between this temperature and that of the sites of perception of the thermal stimulus by the plant. However, development may be more dependent on soil temperature than air temperature (Kliewer, 1975). Morlat (1989) thus verified that the variability in the precocity of the vine, positively correlated with the quality of the harvest and of the wine in the Loire Valley, was mainly explained by differences in temperature of the root zones.

Marselan (Cabernet-Sauvignon x Grenache), has been planted for more than 20 years now in Uruguay. Due to its good agronomic and oenological aptitudes under uruguayan conditions, it is currently the red variety with highest plantation rate. The objective of the study was to identify management practices, aimed at improving quality in highly productive vineyards, different fruit/leaf regulation methods were tested in southern Uruguay.

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

The aim of this work was to evaluate the effect of preharvest application in Monastrell berries using two different types of applications: conventional treatments