Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

In recent years, Uruguay has embraced the Albariño grape variety (Vitis vinifera L.) as one of the most promising for commercial growth. Originally cultivated in Galicia and northern Portugal, Albariño has risen to prominence in the global wine market, driving strong demand and significantly increasing grape prices [1].

The presence of undesirable compounds in wines, such as OTA, biogenic amines and pesticides residues, affects wine quality and can cause health problems for the consumer. The main tool that a winemaker has to reduce their content in the wine is fining. However, some of the fining agents commonly used in the winery can cause allergies or even increase the protein content in the wine, increasing the turbidity. To avoid these problems, the use of plant fibers may be an alternative, such as those from grape pomace[1] or other plant origins.

Esca is a grapevine vascular disease with detrimental consequences on vineyard yield and longevity. Recently, esca leaf symptom development has been shown to result in the occlusion of xylem vessels by tyloses in leaves and stems, leading to hydraulic failure. However, little is known regarding the response of xylem anatomy and stem growth to esca in different varieties . Here we studied the impact of esca leaf symptom development on grapevine physiology, stem growth, and xylem anatomy in two widespread cultivars, Cabernet sauvignon and Sauvignon blanc.

Grapevine yield and wine quality are dependent on good quality vegetative growth and root development. Soils that restrict proper grapevine root development, together with the high cost of establishing a new vineyard, require effective soil preparation to sustain productive vineyards for 25 years. This study reviews soil preparation research conducted over the past 50 years and identifies best practices to remove soil physical and chemical impediments to create optimum conditions for root growth.

Grape phylloxera (Daktulosphaira vitifoliae Fitch) is a major insect pest that has had devastating effects in grape-growing regions worldwide. As a result, using phylloxera-resistant rootstocks is a key component of modern vineyard management.