Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Grapevines are susceptible to freezing damage at temperatures below -5°F during the winter season. Preventing winter injury to grapevines is a major challenge in many grape-producing regions. Conventional methods such as hilling-up soil over graft unions have been developed as winter protection methods for preventing vine loss. However, these practices have drawbacks such as soil erosion, vine damage and crown gall development.

Grape stalks are a byproduct of the winemaking process and represent a valuable and inexpensive source of bioactive compounds. While their direct use in whole bunch fermentation is known, the majority of grape stalks are discarded, posing environmental and economic challenges.

In the context of climate change and the need to reduce inputs, optimising photosynthesis and grapevine performance requires a better understanding of the interactions between water status, nitrogen availability, and source-sink relationships.

Las tendencias actuales del mercado apuntan hacia el consumo de vinos con menor contenido en alcohol, y, por otra parte, de vinos con características especiales y diferenciadoras, siendo los vinos con indicación geográfica o denominación de origen los más demandados.

AIM: Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, wines with low levels of quality compounds.