Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Wine acidity is a parameter of great importance that influences different quality factors of the product such as biological stability or organoleptic characteristics. In the current context of climate change, which gives rise to wines with higher levels of ethanol and lower acidity, the biological acidification with yeast species such as Lachancea thermotolerans could be a solution.
In this work, the effect of the inoculation of different L. thermotolerans on the acidity of wine was studied.

Vine water status has a significant influence on vineyard yield and berry composition (Williams and Matthews, 1990; Williams et al., 1994). It has been hypothesized that the response of plants to soil water deficits may be due to some sort of “root signal” (Davies and Zhang, 1991). This signal probably arises due to the roots sensing a reduction in soil water content or an increase in the mecanical impedance as the soil dries out.

Retsina is a wine deeply rooted in Greek tradition but often misunderstood, largely due to the poor quality associated with past production. Historically, pine resin was used to seal wine transport containers, and over time, its distinctive aroma led to its intentional incorporation into winemaking.

Oxygen management in wine is one of the most significant challenging issues for winemakers.

In some white wines, malolactic fermentation (MLF) is very interesting, and for low pH wines this process is particularly difficult. Although MLF is generally not recommended for sparkling white wine, some winemakers prefer to promote MLF to contribute to organoleptic complexity. Oenococcus oeni is generally the bacterium of choice for MLF.