Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

C-Glucosidic ellagitannins, which are the main polyphenolic compounds in oak heartwood, are extracted by wine during aging in oak barrels. Although such maturing of alcoholic beverages in oak barrels is a multi-centennial practice, very little is known on the impact of these ellagitannins on the organoleptic properties of red wine. The objectives of the present investigation were (i) to isolate oak ellagitannins and to hemisynthesize some made-in-wine flavano-ellagitannins, such as acutissimin A; (ii) to analyse their concentration ranges depending on the cultivar area and (iii) to evaluate their sensory impact on the basis of their human threshold concentrations and dose/response relationships in different types of solutions.

To be accepted by the market and consumers table grapes need to meet certain requirements in terms of physical and chemical quality parameters.

Young white wines should be consumed within a short time after bottling to avoid loss of their fresh, fruity attributes. Shelf-life of white wines can be extended if they are stored under suitable conditions of time and temperature prior to consumption.

En Anjou, une méthode de caractérisation des terroirs viticoles a été développée. Elle utilise un modèle de terrain basé sur la profondeur de sol et son degré d’argilisation. Le modèle concerne des terrains issus principalement de roches mères métamorphiques et éruptives du Massif Armoricain. Cet outil de caractérisation des terroirs viticoles nécessite d’être adapté lorsqu’il s’agit d’ensembles géologiques très différents, en particulier sur sols d’apport et de roches mères tendres et poreuses du Bassin Parisien. Une meilleure compréhension de la réserve hydrique des sols apparaît être un critère important de l’interaction entre le milieu et la plante.

Methyl-jasmonate (MeJA) induces the production of at least 25 compounds with sesquiterpene- like mass spectra in ‘Cabernet sauvignon’. Tost effective concentration of MeJA in stimulating the production of sesquiterpenes was found to be 500 µM if added when the cell suspensions had a PCV of 35 %, and 1000 if added when the cell suspensions had a PCV of 70 %.