Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Climate change is increasing crop evapotranspiration and reducing water availability, especially in the Mediterranean area.

There is a growing trend on the transition from conventional to agroecological management of vineyards. However, the impact of practices, such as reduced-tillage, organic fertilization and cover crops, is not well-understood regarding the soil microbial diversity, and its relationship with the soil physicochemical properties in the subsurface depth near the rooting zone. Soil bacterial diversity is an important contributor towards plant health, productivity and response to environmental stresses. A field experiment was conducted by sampling subsurface soil bacterial community (NGS and qPCR) near to the root zone of Macabeu and Xarel·lo vineyards, located at the Penedes. 3 organic (ECO) and 3 conventional (CON) vineyards, with more than 10 years of respective management were sampled (n=5 each plot). ECO practices did not affect bacterial and fungal abundance but increased significantly the ammonium oxidizing bacteria and alpha-diversity (Inv.Simpson). Interestingly beta-diversity was significantly affected by the management strategy. ANOSIM-tests revealed a significative effect of the management (ecological vs conventional) and plot, on the soil microbial structure (ASV abundance). Main phyla depicted were Proteobacteria, Actinobacteria and Acidobacteria, whose relative abundances were not affected by the management. EdgeR assay revealed a significant increase of Cyanobacteria and decrease of Gemmatimonadetes and Firmicutes phyla in ECO. Interestingly, the grapevine variety was not correlated with the soil microbial community structure. Mantel-test revealed an important correlation (Spearman) of some physicochemical parameters with the soil microbiota structure, in order of importance: texture, EC, pH Ca/Mg, Mg/P, K+, Mg2+, Ca2+, SO42-, and OM. N-NH4 and NTK, which were higher in the ECO managed soils, did not correlated significantly with the soil microbiome population. The results revealed the importance of combining a deep physicochemical characterization of each replicate with the microbial diversity assessment to gain better insights on the relationship between soil microbiome and vineyard management.

L’A.O.C. Coteaux du Languedoc est située dans le Sud de la France, dans la partie Ouest de la bordure méditerranéenne. Elle forme un vaste amphithéâtre largement ouvert sur la mer méditerranée. L’Appellation a été constituée en 1960 par le regroupement de 14 anciennes petites appellations d’origine représentant 55 communes éparpillées dans les départements de l’Aude, de l’Hérault et du Gard. Par la suite, plusieurs extensions successives ont conduit à un ensemble actuellement composé de 168 communes.

The project “Terroir Hesse” works out the main type and characteristics of soil-based terroirs and the resulting wine styles for the hessian wine-growing regions Rheingau and Hessian Bergstrasse.

Aims: Wine typicity is defined as a reflection of varietal origins, cultures and traditions of the wine. These aspects are many times also extremely important when considering a wines quality. However, as climate change occurs the typicity of wines may also change. With the long history of winemaking it is possible to define a wines typicity and how it has changed as climate alters.