Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

In this audio recording of the IVES science meeting 2022, Antonio Graca (Sogrape, Portugal) speaks about smoke taint and climate change. This presentation is based on an original article accessible for free on IVES Technical Reviews.

Changes in climate have been influencing the quality of wine grapes worldwide. The impact of extreme climate events over short periods is increasingly recognized as a serious risk to grape quality and yield quantity. In this study the mitigation effects of a pulsed water spray on vine canopy during heatwave events has been evaluated for maintaining vine condition during the growing season and grape quality. Vines of three varieties (Malbec, Bonarda, and Syrah) under drip irrigation in the UNCuyo experimental vineyard were treated with an overhead pulsed water spray.

Occurrence of freezing temperatures in early spring when grapevine shoots are developing is termed late frost in viticulture. Young green tissues are very sensible to temperatures below zero and damages often lead to important yield and quality losses such as the case in Europe in 2017. An indirect method to avoid late frost damage in vineyards consist in delaying the budburst. Previous research reported similar effects by applying vegetal oil on dormant buds. Here, we tested the application of rapeseed vegetal oil during late winter to delay the budburst on two V.vinifera cultivars of interest in Austria, Grüner Veltliner (GV) and Zweigelt (ZW).

Stomata are microscopic pores mainly located in leaf epidermis, allowing gas exchanges between plants and atmosphere. Stomatal initiation relies on the transcription factor SPEECHLESS which is mainly regulated by the MAP kinase cascade, in turn controlled by small signaling peptides, the Epidermal Patterning Factors (EPF and EPF-Like), namely EPF1, EPF2 and EPFL9. While EPF1 and EPF2 induce the inhibition of SPEECHLESS, their antagonist, EPFL9, stabilizes it, leading to stomatal formation. In grapevine, there are two paralogs for EPFL9, VviEPFL9-1 and VviEPFL9-2. Despite their structural similarity, it remains unclear whether they are differentially regulated and have distinct roles.

Today, a variety of remote sensing tools are used to characterise plant performance. However, the vine is rarely studied, as a major crop specificity is canopy discontinuity. Registered images of the vineyard are anisotropic, therefore difficult to analyse.