terclim by ICS banner
IVES 9 IVES Conference Series 9 In vitro tissue culture as a tool for Croatian grapevine germplasm management

In vitro tissue culture as a tool for Croatian grapevine germplasm management

Abstract

In vitro culture makes it possible to carry out specific studies that would not be possible with whole plants grown in the field or in a greenhouse. Cryopreservation allows long-term preservation without metabolic changes in the plant material and cryotherapy can be efficient in virus elimination, which is a major scientific challenge.
The preculture media of cryopreservation protocols were evaluated on three Croatian grape varieties with different antioxidants (salicylic acid, ascorbic acid and glutathione). The highest growth in vitro was achieved on the medium with the addition of glutathione and the lowest with the addition of salicylic acid. Growth in vitro and regeneration after cryopreservation depended on genotype and health status. The cryopreservation protocols tested (as part of cryotherapy) did not result in sufficient regeneration after cryopreservation (10-15%) in the varieties tested.
Virus elimination was tested for economically important viruses by meristem tip culture in 18 Croatian varieties. In vitro regeneration ranged 6.82-53.22%, but virus elimination was achieved in only three cultivars and was very low (23.8%). In addition, two new grapevine viruses (GVG and GBV-1) were tested. The results showed a low percentage of virus elimination (2%) by meristem tip culture in three-month-old tissue cultures.
Preliminary research activities were carried out by micrografting with Croatian grape varieties. The survival rate was good, but regeneration was difficult to achieve. A more detailed study is in progress. Overall, the presented methods of meristem tip culture, cryopreservation and micrografting should be further evaluated for the Croatian grapevine germplasm to enable wider application.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Zvjezdana Marković1*, Željko Andabaka1, Domagoj Stupić1, Iva Šikuten1, Petra Štambuk1, Edi Maletić1, Jasminka Karoglan Kontić Jasminka1, Darko Vončina2, Darko Preiner1

1 University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb
2 University of Zagreb, Faculty of Agriculture, Department of Plant Pathology, Zagreb

Contact the author*

Keywords

Vitis vinifera L., cryopreservation, preculture with antioxidants, virus elimination, meristem culture, regeneration

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Grape phylloxera leaf-feeding populations in commercial vineyards – a new biotype ?

Grape Phylloxera (Daktulosphaira vitifoliae Fitch) ordinarily has great difficulty establishing leaf galls on the European Grapevine (VitisviniferaL.). Yet populations of leaf-feeding Phylloxera are increasingly being observed throughout commercial vineyards world-wide. Effective plant protection strategies including quarantine actions are currently missing to fight, grape phylloxera populations in affected vineyards and combat linked negative effects on vines and yield. Contrary to the otherwise mandatory continuous infestation pressure from externally established populations (e.g. from populations developed on rootstock foliage or other interspecific hybrids, these leaf-feeding populations seem to establish themselves annually.

Revisión de estudios sobre suelos vitícolas de las tierras del Jerez

Dada la importancia de los suelos y de los substratos geológicos en la zonificación vitivinícola, los autores realizan una revisión de estudios sobre las formaciones más importantes en la D.O. Jerez-Xérès-Sherry y Manzanilla-Sanlúcar de Barrameda.

Frost variability in the Champagne vineyard: probability calendar

Dans le vignoble champenois, le risque thermique associé au gel des bourgeons au printemps et en hiver est très mal connu et ne peut être envisagé qu’à l’échelle locale, en raison d’une variabilité spatiale forte. L’objectif de l’étude est d’appréhender ce risque de façon fiable et pluri locale en utilisant le réseau de stations météos récemment implanté.

Combined high-resolution chromatography techniques and sensory analysis as a support decision system tool for the oenologist

One of the main challenges in the wine industry is to understand how different wine processing techniques and practices can influence the overall quality of the final product.

Aspect juridiques des terroirs

Le “terroir” est dans tous les discours, les articles, les étiquettes et les publicités. Le voca­ble est en situation d’utilisation euphorique. Indiscutablement l’emploi historique est agri­cole, puis viticole, mais il n’est jamais juridique.