terclim by ICS banner
IVES 9 IVES Conference Series 9 The grapevine single-berry clock, practical tools and outcomes 

The grapevine single-berry clock, practical tools and outcomes 

Abstract

The dynamic sequence of physiological events along the three-months of berry development from anthesis to ripe stage has been thoroughly investigated. Most studies were performed on average samples, taking care to crush enough fruits to fairly represent the overall trend of the future harvest. However, phenological stages like 30% caps off (EL25) highlights the asynchronous nature of this population. Consequently, softening, onset of sugar accumulation and coloration were melted by asynchrony in a developmental mumbo jumbo, until their respective timing could be clarified by single berries approaches. To alleviate any statistical bias from pooling unsynchronized fruits, we have gathered a set of approaches on single berries, including non-destructive analysis of time-lapse images, Near InfraRed Spectrometry, basic phenotyping, transcriptomics and metabolomics. In this pertinent reference system, the sugar and acid pathways noticeably accelerated and metabolic transitions were sharpened. Transcripts encoding membrane transporters abruptly switched on during either growth phases were identified, enlightening the special design and bioenergetics of the phloem unloading and vacuolar accumulation pathways, in full quantitative agreement with our new appraisal of the sugar/acidity ratio. Single berries did not depart from strict developmental paths on PCAs from transcriptomic or metabolomic data. It led to the identification of a very small set of genes differentially expressed between clones, without interfering with developmentally regulated ones. This study shows that single berry omics alleviates random noise and temporal ambiguities inherent to mixed fruits, thereby improving the accuracy of the molecular clocks to just a few days. 

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Mengyao Shi1, Flora Tavernier1, Victoria Lesbats-Sichel1, Stefania Savoi2, Benoit Daviet3, Maxence Cafier3 , Philippe Hugueney4, Raymonde Baltenweck4, Christian Fournier3, Vincent Segura1,5, Laurent Torregrosa3*, and Charles Romieu1*, 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpeller, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Heat-stress responses regulated via a MYB24-MYC2 complex

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages.

THE IMPACT OF NON-SACCHAROMYCES YEASTS ON THE WHITE WINE QUALITY

Selected strains of non-Saccharomyces yeasts showed a positive effect on sensory characteristics and aromatic complexity of wine. A sequential microbial culture of non-Saccharomyces and S. cerevisiae species is usually inoculated due to poorer fermentability of non-Saccharomyces species. The aim of the study was to investigate the role of non-Saccharomyces yeasts in the production of white wines. We evaluated how individual combinations of sequential inoculations of non-Saccharomyces and S. cerevisiae species affect the aromatic compounds (volatile thiols and esters) and sensory characteristics of the wines.

Revisiting esters hydrolysis in young white wines

Esters play an essential role in the young white wines’ fruity expression, particularly the groups of ethyl esters of fatty acids (EEFAs) and higher alcohol acetates (HAAs) [1]. However, generally, these groups of esters decrease relatively fast during the first two years of ageing [1, 2].

INFLUENCE OF WINEMAKING VARIABLES AND VINEYARD LOCATIONS ON CHEMICAL AND SENSORY PROFILES OF SOUTH TYROLEAN PINOT BLANC

Pinot Blanc, an important grape variety grown in some mountain areas of Northern Italy such as South Tyrol over the last decades, with its cultivation covering 10.3% of the total vineyards, has compatible climatic conditions (e.g. heat requirements) which are normally found in the geographical areas of the mountain viticulture [1,2,3,4]. Climatic changes are hastening the growth of this variety at higher elevations, particularly for the production of high quality wine.

Can wine composition predict quality? A metabolomics approach to assessing Pinot noir wine quality as rated by experts

The perception of wine quality is determined by the assessment of multiple sensory stimuli, including aroma, taste, mouthfeel and visual aspects. With so many different parameters contributing to the overall perception of wine quality, it is important to consider the contribution of all metabolites in a wine when attempting to relate composition to quality.