terclim by ICS banner
IVES 9 IVES Conference Series 9 The grapevine single-berry clock, practical tools and outcomes 

The grapevine single-berry clock, practical tools and outcomes 

Abstract

The dynamic sequence of physiological events along the three-months of berry development from anthesis to ripe stage has been thoroughly investigated. Most studies were performed on average samples, taking care to crush enough fruits to fairly represent the overall trend of the future harvest. However, phenological stages like 30% caps off (EL25) highlights the asynchronous nature of this population. Consequently, softening, onset of sugar accumulation and coloration were melted by asynchrony in a developmental mumbo jumbo, until their respective timing could be clarified by single berries approaches. To alleviate any statistical bias from pooling unsynchronized fruits, we have gathered a set of approaches on single berries, including non-destructive analysis of time-lapse images, Near InfraRed Spectrometry, basic phenotyping, transcriptomics and metabolomics. In this pertinent reference system, the sugar and acid pathways noticeably accelerated and metabolic transitions were sharpened. Transcripts encoding membrane transporters abruptly switched on during either growth phases were identified, enlightening the special design and bioenergetics of the phloem unloading and vacuolar accumulation pathways, in full quantitative agreement with our new appraisal of the sugar/acidity ratio. Single berries did not depart from strict developmental paths on PCAs from transcriptomic or metabolomic data. It led to the identification of a very small set of genes differentially expressed between clones, without interfering with developmentally regulated ones. This study shows that single berry omics alleviates random noise and temporal ambiguities inherent to mixed fruits, thereby improving the accuracy of the molecular clocks to just a few days. 

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Mengyao Shi1, Flora Tavernier1, Victoria Lesbats-Sichel1, Stefania Savoi2, Benoit Daviet3, Maxence Cafier3 , Philippe Hugueney4, Raymonde Baltenweck4, Christian Fournier3, Vincent Segura1,5, Laurent Torregrosa3*, and Charles Romieu1*, 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpeller, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Chemical composition of cool-climate Sauvignon blanc grape skins clones during ripening

Sauvignon blanc is the most important variety in cool valleys in central Chile accounting 15,522 ha which corresponds to 42.4% of the cultivated surface with white varieties in Chile

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease.

Natural variability and vine-growers behaviour

Le vigneron est confronté à une variabilité naturelle omniprésente, liée au millésime et aux facteurs pédoclimatiques. Depuis 10 ans, en Champagne, la relation qu’entretient le vigneron avec l’espace a évolué. Les exemples d’entreprises collectives à vocation territoriale se sont multipliés : gestion de l’hydraulique viticole, maillages de groupements de conseil viticole (Magister), sites en confusion sexuelle, réseau maturation, analyses de sols par secteur, …

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.