A comprehensive and accurate annotation for the grapevine T2T genome 

Climate change is having an unprecedented impact on the wine industry, which is one of the major agricultural sectors around the world. Global warming, combined with the variation in rainfall patterns and the increase in frequency of extreme weather events, is significantly influencing vine physiology and exposing, more frequently, plants to severe biotic and abiotic stresses. This represents a challenge for viticulturists who need to take complex decisions to adjust vineyard management and achieve oenological goals.

Climate is one of the main drivers of spatial and temporal variability in grapevine physiology and therefore a key determinant of grape composition and final wine value. The world has warmed 1.1 °C since pre-industrial times, and the latest IPCC report indicates an additional 0.5 to 1.3 °C of warming by mid-century with continental locations warming at a greater rate than the oceans.

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fungicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidimensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature).

Red wines can sometimes exhibit undesirable green, herbaceous, and vegetative aromas, negatively impacting their sensory profile and consumer acceptance.

Wine fraud, encompassing counterfeiting and adulteration, poses a significant threat to the wine industry, resulting in annual losses totalling billions of dollars.