terclim by ICS banner
IVES 9 IVES Conference Series 9 Understanding the expression of gene families involved in anthocyanin biosynthesis during berry ripening: Tannat as a case study

Understanding the expression of gene families involved in anthocyanin biosynthesis during berry ripening: Tannat as a case study

Abstract

The quality of wine is assessed, among other things, by its color, which is mainly due to its anthocyanin content. These pigments are polyphenols that give red, purple and blue hues depending on the relative proportion of anthocyanins produced by the action of flavonoid 3’5′ hydroxylase (delphinidin-3-glucoside, petunidin-3-glucoside, malvidin-3-glucoside) or flavonoid 3′ hydroxylase (cyanidin-3-glucoside, peonidin-3-glucoside). To study the genes involved in this biosynthetic pathway, we focused on Vitis vinifera cv. Tannat, known for producing wines with higher anthocyanin content and darker purple color compared to most red grape varieties. In this work, we have performed RNA-Seq analysis of skins during berry development, taking green and red berries at 50% veraison as separate samples, as an experimental strategy to focus on the differential expression of genes of interest. Several novel (not previously annotated in the Pinot noir reference genome PN40024 12X) and varietal (not in the reference genome) genes showed differential expression associated with color change during veraison. Expression of a novel F3’5’H gene and a varietal F3’H gene was confirmed by RT-qPCR in the same samples used for RNA-Seq and in another growing season. This work establishes the specific expression fingerprint for gene families (CHS, CHI, F3H, F3’H, F3’5’H, DFR, LDOX, 3GT, OMT, MYBA1, MYBA2, MYBA3) directly involved in anthocyanin biosynthesis and regulation during Tannat veraison.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Cecilia C. Da Silva1*, Eduardo Boido2, Carina Gaggero3, Massimo Delledonne4, Francisco Carrau2

1 PDU Espacio de Biología Vegetal del Noreste, sede Tacuarembó, CENUR Noreste, Universidad de la República, Tacuarembó, Uruguay
2 Área Enología y Biotecnología de Fermentaciones, Facultad de Química, Universidad de la República, Montevideo, Uruguay
3 Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
4 Centro di Genomica Funzionale, Dipartimento di Biotecnologie, Universitá degli Studi di Verona, Verona, Italy

Contact the author*

Keywords

Anthocyanins, Vitis vinifera, Tannat, RNA-Seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Environment friendly nutrition supplying and soil cultivation methods applicable in the upper zone of hillside vineyards

Somló is Hungary’s smallest wine district, however one of the best producing white wines. The majority of vineyard areas are located on the slopes of Somló-hill, situated at the point where the Kisalföld meets Bakonyalja

Landscape qualities and keys for action

Parallèlement à la connaissance des aptitudes viticoles, le terroir témoigne d’une identité locale, d’une spécificité des conditions de productions et d’une originalité des lieux.

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

1H-NMR-based Untargeted Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

Untargeted metabolomics has proven to be an effective method to study the impact of the terroir on metabolic profile of wines. In this context, the aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through 1H-NMR metabolomics combined with chemometrics.Grapes from Nero d’Avola L. red cultivar cultivated on four different soil types were separately vinified to obtain four different red wines.One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz

Three proximal sensors to estimate texture, skeleton and soil water storage in vineyards

Proximal sensors are becoming widely used in precision viticulture, due to the quick, easy and non-invasive identification of soil spatial variability. The apparent soil electrical conductivity (ECa) is the main parameter measured by sensors, which is correlated to many factors, like soil water content, salinity, clay content and mineralogy, rock fragments, bulk density, and porosity.