Defining gene regulation and co-regulation at single cell resolution in grapevine

From a fertility standpoint, the vine has to extract from the soil mineral substances necessary for its existence. However, the amount of certain available nutrients does not always correspond to a proportional increase in quality.

To alter the vineyard microclimate and produce quality wine under a semi-arid climate, black geotextile inter-row mulch (M) was applied for two vintages (2016-2017). The grapes were sampled at three growing stages to conduct the untargeted metabolome and transcriptome analysis. The upregulated genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered severe heat stress, resulting in lower sugar and higher acids at harvest. The integration of metabolome and transcriptome analysis identified the key genes responsible for the enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes and norisoprenoids concentrations in M grapes.

Climate change is having a significant impact on the wine industry through more regular drought conditions, fires, and heat events, leading to crop loss. Furthermore, these events can reduce overall quality of the fruit, even when crop yields are not impacted. Anthocyanins are considered one of the most important classes of compounds for red wine production and are known to be sensitive to vine water status and heat events.

Terroir is about the link between wine and its origin. It has long been understood by sensory evaluation that the taste of wine from a given variety can be related to its origins. Specific organoleptic characteristics of wine are influenced by environmental factors such as soil and climate. By deconstructing the effect of measurable soil and climate parameters on grape and wine aroma compounds,

Riesling represents the most widely cultivated grape variety in Germany and is therefore of particular economic interest. During recent years an increase in the petrol-note as well as in undesirable bitter and adstringent notes has been reported. These changes are most likely linked to increasing temperature and sunlight exposure of grapes due to climate changes.
The “petrol note” is caused by the formation of the C13-norisoprenoid 1,1,6-trimethyl-1,2-dihydronaphthalin (TDN), which originates from acid-labile precursors formed by the carotenoid degradation in the grape.