Defining gene regulation and co-regulation at single cell resolution in grapevine

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

Tannins are a heterogenous class of polymeric phenolics found in grapes, oak barrels and wine. In red wine tannins are primarily responsible for astringency, though they also have an important role in reacting with and stabilizing pigments. There are numerous sub-classes of tannins found in wine but they all share structural heterogeneity within each sub-class, with varied polymer composition, configuration and length.

Numerous methodologies exist for the quantification of tannins, however, protein precipitation using bovine serum albumin has proved itself useful due to its strong correlation to the sensory perception of astringency and the basic instruments required for the method. Though the method can yield valuable insights into tannin composition, it cannot be automated easily and necessitates well-trained personnel.

The adoption of PIWI (Pilzwiderstandsfähige) grape cultivars, bred for resistance to fungal diseases, is a transformative step towards sustainable winemaking.

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.

The spotted lanternfly (SLF; Lycorma delicatula) is a phloem-feeding polyphagous insect invasive to the Eastern U.S.. Since its first detection in Pennsylvania (U.S.) in 2014, large infestations and economic damage (e.g., decreased yield, vine decline, greater pesticide use) have been reported in an increasing number of vineyards, threatening the sustainability and growth of the wine industry in infested regions. Our team has been investigating the impacts of SLF phloem-feeding on physiological processes, fruit production, juice, and wine composition of different grape cultivars, and also evaluated if the SLF can transmit important grapevine pathogens. In addition, we are working closely with stakeholders to better enumerate the economic damage caused by this pest. These findings will provide relevant information to grape and wine producers to help identify action thresholds and develop a more targeted integrated pest management program.