Defining gene regulation and co-regulation at single cell resolution in grapevine

Les préoccupations actuelles autour des paysages viticoles vont au-delà des clichés promotionnels développés par les stratégies marketing. En effet, les paysages sont aujourd’hui au cœur d’une demande sociale croissante qui se traduit par différentes lois (la loi paysage de 1993, le paysage reconnu comme patrimoine commun de la nation par la loi n°95-101, la création du Conseil national du paysage par arrêté du 8/12/2000).

Botrytis cinerea is a necrotrophic pathogen that causes one of the most serious diseases of the grapevine (Vitis vinifera), grey mold or Botrytis bunch rot. In Champagne, the Botrytis cinerea disease leads to considerable economic losses for winemakers and wines exhibit organoleptic defaults.

La salubrité du milieu et des aliments intervient de plus en plus lourdement, et souvent négativement, sur la santé de l’homme, aussi bien sur l’individu que sur la société tout entière.

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

In California, little training in sprayer calibration or pesticide drift management is required to apply pesticides. Yet, there is a need to maximize pesticide efficacy and minimize drift. Therefore, our team is developing a training course on airblast application best practices. We distributed a survey to identify current practices and used importance-performance analysis to interpret responses to the importance of spray related topics and satisfaction with previous training.