Defining gene regulation and co-regulation at single cell resolution in grapevine

Aim: The aim of this study was to quantify key berry sugar accumulation traits and characterize their plasticity in response to climate variation from data collected from different cultivars over seven years from an experimental vineyard.

La prise en compte des paysages viticoles dans le développement durable ou l’aménagement du territoire est un thème non négligeable pour la valorisation de la filière viti-vinicole à l’échelle d’une exploitation ou d’une A.O.C.

The quality of red wine depends on the composition of polyphenols influencing wine color and taste. The question is, how much we must fear over extraction, especially of seed tannins, under cool climate conditions. The extraction of polyphenols from grape skins and grape seeds were investigated for the grape varieties Cabernet Sauvignon and Pinot noir

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Vigor declines in older vineyards and poor vine establishment in replant situations have been attributed to plant-parasitic nematodes. The northern root-knot nematode, Meloidogyne hapla, is the most prevalent plant-parasitic nematode species found in Washington wine grape vineyards. Management for nematodes in established vineyards is limited to the application of post-plant nematicides. We are evaluating new nematicides that are currently not registered in grape for their efficacy in controlling M. hapla and a part of that evaluation includes improving the alignment of nematicide application timing with the vulnerable second-stage juvenile (J2) life stage of M. hapla.