terclim by ICS banner
IVES 9 IVES Conference Series 9 Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Abstract

Drought and heat stress will pose challenges for the future of viticulture and wine quality, as grapevine biological processes are pushed beyond their optimum conditions. Efforts are increasing to study and predict the effects of drought spells and heatwaves on grapevine physiology and resulting harvest quality. This calls for the development of adequate systems to induce and manipulate the required stress, especially in open field trials where conditions are more difficult to control. We present a semi-controlled system for studying drought and heat stress in grapevine in the field. The system uses automatic weighing mini-lysimeters to record whole-plant evapotranspiration throughout the growing season and manage deficit irrigation programs for drought trials, as well as infrared lamps to heat the cluster zone above ambient temperature. Drought stress was imposed at 25% soil field capacity for 9 days, during which a heat stress of 5 days was induced with infrared lamps set to 800W at 30cm from the fruiting cane. Grapevine ecophysiology was monitored throughout the experimental period. The system successfully allowed us to control grapevine evapotranspiration, lowering both leaf stomatal conductance (gsw) and midday stem water potential (Ψstem), as well as increase berry surface temperatures, with a mean increment of 3.6°C. These results provided insight into the efficacy of the system in imposing stress in the field, as well as into possible further improvements for the setup, taking into consideration the constraints of the system as well as undesired effects of changing weather during the experiment.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Jacopo Innocenti1*, Elena Farolfi1, Francesco Flagiello1, Rudi Rizzoli1, Soma Laszlo Tarnay1, Astrid Forneck1, José Carlos Herrera1

1 Institute for Viticulture and Pomology, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz Straße, 3430, Tulln an der Donau, Austria

Contact the author*

Keywords

abiotic stress, climate change, heatwaves, phenotyping, method development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Anthocyanin and trans-resveratrol accumulation is associated with abscisic acid and methyl jasmonicanthocyanin and trans-resveratrol accumulation is acid in berry skin of vitis vinifera L. Cvs. Malbec, Bonarda, Syrah, Cabernet sauvignon, and Pinot noir

Red grapes contain significant amounts of phenolic compounds, known to contribute to wine quality and to provide important health benefits. Berry skin phenolics can be elicited by plant hormones. The aim of this work was to increase the content of anthocyanins and trans-resveratrol in five red varieties cultured in Argentina: Malbec (M), Bonarda (B), Syrah (S), Cabernet Sauvignon (CS), and Pinot Noir (PN), in two different growing regions: Santa Rosa (SR) and Valle de Uco (VU), by applying a post-veraison hormonal treatment with abscisic acid (ABA) and methyl jasmonate (MeJA).

Dormancy conundrum: thermal requirements plasticity to reach budburst may be explained by annual environmental dynamics

Deciphering grapevine dormancy is crucial in the current context of climatic challenges: advancing budburst phenology and increased late frost probabilities, observed in the last decades and expected to further increase, require deeper understanding. Beyond higher mean temperatures, abiotic stresses such as water deficit have also been emphasized as actors. In this framework, we aimed at exploring new methodologies for tracking dormancy cycle and testing the interplay on its regulation of temperature dynamics and drought.
In a first experiment, twenty-one Vitis vinifera varieties were monitored during ecodormancy and budburst over three years.

The use of cation exchange resins for wine acidity adjustment: Optimization of the process and the effects on tartrate formation and oxidative stability

Acidity adjustments are key to microbial control, sensory quality and wine longevity. Acidification with cation exchange resins -in acid cycle- offers the possibility to reduce the pH by exchanging wine cations, such as potassium (K+), for hydrogen ions (H+). During the exchange process, the removal of potassium and calcium ions contributes to limiting the formation of tartrate salts, thus offering an alternative solution to conventional methods for tartrate stability. Moreover, the reduction of wine pH and the removal of metals catalyzers (e.g. iron) could positively impact the wine’s oxidative stability. Therefore, the aims of this work were (a) to optimize the ion exchange process by testing different volumes and concentrations of sulfuric acid (H2SO4) during the acid cycle, (b) evaluate the effects of the ion exchange process on the formation of tartrate salts, and (c) analyze the oxidative stability of the treated wines.

S. CEREVISIAE AND O. ŒNI BIOFILMS FOR CONTINUOUS ALCOHOLIC AND MALOLACTIC FERMENTATIONS IN WINEMAKING

Biofilms are sessile microbial communities whose lifestyle confers specific properties. They can be defined as a structured community of bacterial cells enclosed in a self-produced polymeric matrix and adherent to a surface and considered as a method of immobilisation. Immobilised microorganisms offer many advantages for industrial processes in the production of alcoholic beverages and specially increasing cell densities for a better management of fermentation rates.

MICROFLUIDIC PLATFORM FOR SORTING YEAST CELLS ACCORDING TO THEIR MORPHOLOGY

In this work we briefly present a microfluidic device aiming to sort yeast cells according to their morphology. The technology is based upon microfluidic chips made out of Polydimethylsiloxane and glass using soft lithography processes and replica molding. The microfluidic device was used for encapsulating single yeast cells in liquid droplets containing growth medium. Liquid droplet containing yeast cells were sorted using a real time imaging and decision-making process.