Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

This study examines the impact of wine tourism development on the sustainable competitive advantage of Spanish wineries, while also exploring the mediating roles of winery image and winery reputation in this relationship.

Aims: Berry composition and wine sensory characteristics reflect the origin of grape production and seasonal climatic conditions. The aim of this study was to compare berry and wine sensory characteristics from two contrasting soil types where the vineyard climate, geography, topography, vine and management factors were not different.

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

Stilbenes, a kind of non-flavonoid phenolic compounds, have been reported to be responsible for various beneficial effects. Their biological properties include antibacterial and antifungal effects, as well as cardioprotective, neuroprotective and anticancer actions (Guerrero et al. 2009).Several strategies can be used to increase stilbene content in grapes and one of them could be the use of elicitors such as methyl jasmonate. The use of this elicitor has been proven to be efficient in the production of secondary metabolites which increases the quality of wines, but its use also has some drawbacks such as its low water solubility, high volatility, and its expensive cost (Gil-Muñoz et al. 2021).

Water status impact in viticulture has been widely explored, as it strongly affects grapevine physiology and grape chemical composition. It is considered as a key component of vitivinicultural terroir. Most of the studies concerning grapevine water status have focused on either physiological traits, or berry compounds, or traits involved in wine quality. Here, the response of grapevine to water availability during the ripening period is assessed through non-targeted metabolomics analysis of grape berries by ultra-high resolution mass spectrometry. The grapevine water status has been assessed during 2 consecutive years (2019 & 2020), through carbon isotope discrimination on juices from berries collected at maturity (21.5 brix approx.) for 2 Vitis vinifera cv. Pinot noir (PN) and Chardonnay (CH). A total of 220 grape juices were collected from 5 countries worldwide (Italy; Argentina; France; Germany; Portugal). Measured δ13C (‰) varied from -28.73 to -22.6 for PN, and from -28.79 to -21.67 for CH. These results also clearly revealed higher water stress for the 2020 vintage. The same grape juices have been analysed by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Liquid Chromatography coupled to Mass Spectrometry (LC-qTOF-MS), leading to the detection of up to 4500 CHONS containing elemental compositions, and thus likely tens of thousands of individual compounds, which include fatty acids, organic acids, peptides, phenolics, also with high levels of glycosylation. Multivariate statistical analysis revealed that up to 160 elemental compositions, covering the whole range of detected masses (100 –1000 m/z), were significantly correlated to the observed gradients of water status. Examples of chemical markers, which are representative of these complex fingerprints, include various derivatives of the known abscisic acid (ABA), such as phaesic acid or abscisic acid glucose ester, which are significantly correlated with higher water stress, regardless of the variety. Cultivar-specific behaviours could also be identified from these fingerprints. Our results provide an unprecedented representation of the metabolic diversity, which is involved in the water status regulation at the grape level, and which could contribute to a better knowledge of the grapevine mitigation strategy in a climate change context.