terclim by ICS banner
IVES 9 IVES Conference Series 9 Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Abstract

Drought and heat stress will pose challenges for the future of viticulture and wine quality, as grapevine biological processes are pushed beyond their optimum conditions. Efforts are increasing to study and predict the effects of drought spells and heatwaves on grapevine physiology and resulting harvest quality. This calls for the development of adequate systems to induce and manipulate the required stress, especially in open field trials where conditions are more difficult to control. We present a semi-controlled system for studying drought and heat stress in grapevine in the field. The system uses automatic weighing mini-lysimeters to record whole-plant evapotranspiration throughout the growing season and manage deficit irrigation programs for drought trials, as well as infrared lamps to heat the cluster zone above ambient temperature. Drought stress was imposed at 25% soil field capacity for 9 days, during which a heat stress of 5 days was induced with infrared lamps set to 800W at 30cm from the fruiting cane. Grapevine ecophysiology was monitored throughout the experimental period. The system successfully allowed us to control grapevine evapotranspiration, lowering both leaf stomatal conductance (gsw) and midday stem water potential (Ψstem), as well as increase berry surface temperatures, with a mean increment of 3.6°C. These results provided insight into the efficacy of the system in imposing stress in the field, as well as into possible further improvements for the setup, taking into consideration the constraints of the system as well as undesired effects of changing weather during the experiment.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Jacopo Innocenti1*, Elena Farolfi1, Francesco Flagiello1, Rudi Rizzoli1, Soma Laszlo Tarnay1, Astrid Forneck1, José Carlos Herrera1

1 Institute for Viticulture and Pomology, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz Straße, 3430, Tulln an der Donau, Austria

Contact the author*

Keywords

abiotic stress, climate change, heatwaves, phenotyping, method development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

New understanding on sulfites reactivity in wine

Sulfur dioxide is widely used during winemaking as an antioxidant and antimicrobial agent. Bisulfite (HSO3−), the predominant form of SO2 at wine pH, reacts with several wine components forming sulfonated adducts.

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.

Unravel the underlying mechanisms of delaying ripening techniques in grapevine

In a scenario of changing climate conditions, grapevine is significantly affected at multiple levels. Advancements in phenology and berry ripening, however, are the major dynamics of the generalized increase in average temperature and evaporative demand, negatively affecting berry quality and productivity. The aim of this work was to unravel the underlying mechanisms of bunch-zone auxin application (NAA; 1-Naphthaleneacetic acid) and source-limiting canopy management approaches in delaying berry ripening. In randomized block design experiments, control vines were compared to vines treated with NAA, subjected to apical-to-bunch defoliation or antitranspirant application (n=10-to-42 plants per treatment).

Heat-stress responses regulated via a MYB24-MYC2 complex

Throughout the growing season, grapevine frequently encounters environmental challenges associated with heat and light radiation stress, especially during the ripening stage, thereby constraining the yield and quality of berries. MYB24 has been previously proposed to control light responses during late fruit ripening stages, and it has been found to require the co-factor MYC2. We have generated transcriptomic data from grapevine leaves transiently co-transformed with MYB24 and MYC2. Differential expression analysis revealed 179 up-regulated genes (URGs). Considering tissue specificity, where MYB24 is specifically and highly expressed in flowers and late-ripening berries, the expression of these URGs was explored using a previously published Berry Development Atlas gathering berry development data of cv. ‘Pinot Noir’ and ‘Cabernet Sauvignon’ in different vintages.

Contribution of very high resolution satellite remote sensing to the mapping of harvest zones in the Maipo Valley (Chile)

Les images de très haute résolution spatiale sont utilisées depuis peu en viticulture comme une aide à la cartographie des zones de vendanges. A partir d’images multispectrales de très haute résolution spatiale IKONOS (résolution 4 m) et SPOT-5 en supermode (résolution 2.5 m), on propose ici une démarche de segmentation d’une région de vignoble en zones de vendanges.