terclim by ICS banner
IVES 9 IVES Conference Series 9 Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Development of a semi-controlled setup for manipulating drought and heat stress in open field trials

Abstract

Drought and heat stress will pose challenges for the future of viticulture and wine quality, as grapevine biological processes are pushed beyond their optimum conditions. Efforts are increasing to study and predict the effects of drought spells and heatwaves on grapevine physiology and resulting harvest quality. This calls for the development of adequate systems to induce and manipulate the required stress, especially in open field trials where conditions are more difficult to control. We present a semi-controlled system for studying drought and heat stress in grapevine in the field. The system uses automatic weighing mini-lysimeters to record whole-plant evapotranspiration throughout the growing season and manage deficit irrigation programs for drought trials, as well as infrared lamps to heat the cluster zone above ambient temperature. Drought stress was imposed at 25% soil field capacity for 9 days, during which a heat stress of 5 days was induced with infrared lamps set to 800W at 30cm from the fruiting cane. Grapevine ecophysiology was monitored throughout the experimental period. The system successfully allowed us to control grapevine evapotranspiration, lowering both leaf stomatal conductance (gsw) and midday stem water potential (Ψstem), as well as increase berry surface temperatures, with a mean increment of 3.6°C. These results provided insight into the efficacy of the system in imposing stress in the field, as well as into possible further improvements for the setup, taking into consideration the constraints of the system as well as undesired effects of changing weather during the experiment.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Jacopo Innocenti1*, Elena Farolfi1, Francesco Flagiello1, Rudi Rizzoli1, Soma Laszlo Tarnay1, Astrid Forneck1, José Carlos Herrera1

1 Institute for Viticulture and Pomology, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz Straße, 3430, Tulln an der Donau, Austria

Contact the author*

Keywords

abiotic stress, climate change, heatwaves, phenotyping, method development

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A look back at 20 years of exploring the future of the vines and wines sector

What if, in 25 years, most wines were dealcoholized and flavored ? What if vines were only cultivated to combat erosion, store carbon, and provide anthocyanins…? What if climate change completely changed the list of vine varieties cultivable for wine production in France? What if food stores had completely disappeared in favor of virtual platforms? And if… because the long-term future is not predetermined and therefore not knowable, because the future is open to several possibilities, because the future does not emerge from nothing but from the present which conceals heavy trends and weak signals, prospective approaches make it possible to consider the room for maneuver that actors have to promote the advent of a future, which we can hope to be chosen, at least in part.

Hierarchy of the interactions between physical and biological parameters intervening in the Pyrenean Gascon foothill vineyard

Un travail sur les A.O.C. du piémont pyrénéen occidental permet de construire une hiérarchie de paramètres climatiques, géo-pédologiques, morphologiques, de saisir leurs niveaux d’interaction et d’élaborer une méthodologie pour proposer un zonage

Colloidal stabilization of young red wine by Acacia Senegal gum: the major implication of protein-rich arabinogalactan-proteins

Acacia senegal gum (Asen) is an edible dried gummy exudate [1] added in young red wines to ensure their colloidal stability, precluding the precipitation of the coloring matter. Asen macromolecules, belonging to the arabinogalactan-protein (AGP) family [2], are hyperbranched, charged and amphiphilic heteropolysaccharides composed especially of sugars (92-96 %) and a small fraction of proteins (1-3 %). Asen is defined as a continuum of macromolecules that could be separated into three fractions by hydrophobic interaction chromatography (HIC) [3-4]. HIC-F1 (85-94 % of Asen), HIC-F2 (6-18 % of Asen) and HIC-F3 (1-3 % of Asen) are named and classified in that order according to their protein content, and then a growing hydrophobicity. The efficiency of Asen towards the coloring matter instability is evaluated according to an “efficacy test” that consists to determine the Asen quantity required to prevent the flocculation by calcium of a colloidal iron hexacyanoferrate solution (International Oenological Codex).

Genotypic variability in root architectural traits and putative implications for water uptake in grafted grapevine

Root system architecture (RSA) is important for soil exploration and edaphic resources acquisition by the plant, and thus contributes largely to its productivity and adaptation to environmental stresses, particularly soil water deficit. In grafted grapevine, while the degree of drought tolerance induced by the rootstock has been well documented in the vineyard, information about the underlying physiological processes, particularly at the root level, is scarce, due to the inherent difficulties in observing large root systems in situ. The objectives of this study were to determine genetic differences in the root architectural traits and their relationships to water uptake in two Vitis rootstocks genotypes (RGM, 140Ru) differing in their adaptation to drought. Young rootstocks grafted upon the Riesling variety were transplanted into cylindrical tubes and in 2D rhizotrons under two conditions, well watered and moderate water stress. Root traits were analyzed by digital imaging and the amount of transpired water was measured gravimetrically twice a week. Root phenotyping after 30 days reveal substantial variation in RSA traits between genotypes despite similar total root mass; the drought-tolerant 140Ru showed higher root length density in the deep layer, while the drought-sensitive RGM was characterised by shallow-angled root system development with more basal roots and a larger proportion of fine roots in the upper half of the tube. Water deficit affected canopy size and shoot mass to a greater extent than root development and architectural-related traits for both 140Ru and RGM, suggesting vertical distribution of roots was controlled by genotype rather than plasticity to soil water regime. The deeper root system of 140Ru as compared to RGM correlated with greater daily water uptake and sustained stomata opening under water-limited conditions but had little effect on above-ground growth. Our results highlight that grapevine rootstocks have constitutively distinct RSA phenotypes and that, in the context of climate change, those that develop an extensive root network at depth may provide a desirable advantage to the plant in coping with reduced water resources.

A tool for catching mice in wine: development and application of a method for the detection of mousy off-flavour compounds in wine

Over the past two years, the AWRI has received 69 wine samples suspected of being affected by mousy off-flavour. The character has been mostly observed in white wines.