terclim by ICS banner
IVES 9 IVES Conference Series 9 Rootstock x environment interaction shapes shoot system phenotypic variation in grafted ‘Chambourcin’

Rootstock x environment interaction shapes shoot system phenotypic variation in grafted ‘Chambourcin’

Abstract

Recent advances in phenomics and transcriptomics have the enhanced capacity for understanding how clonally propagated perennial crops like grapevines respond to their environments seasonally and over the course of multiple years. Because most grapevines are grafted, above-ground grapevine traits reflect scion genotype and its interaction with the local environment. In addition, traits expressed by the scion reflect rootstock genotype and how that rootstock is interacting with its environment seasonally and across years. To investigate rootstock x environment interaction on shoot systems in grafted grapevines we characterized comprehensive phenotypic variation in an experimental vineyard in Mount Vernon, Missouri, USA where the grapevine cultivar ‘Chambourcin’ is growing on its own roots and is grafted to three different rootstocks (‘1103P’, ‘3309C’, ‘SO4’).  This set of four combinations is replicated 72 times in a randomized block experimental design with an irrigation treatment. Over the course of three years we quantified leaf elemental concentration, leaf transcriptome, leaf metabolome and epigenome, among others. Analyses in the ‘Chambourcin’ vineyard reveal extensive and dynamic phenotypic variation in ‘Chambourcin’ that reflects complex interactions among rootstock genotype, irrigation, season, and year. Specific effects of rootstock genotype on gene expression and elemental concentration were detected and vary with season and year. Variation in ion concentrations is also influenced by leaf position along the vine. This comprehensive, multi-year project demonstrates the importance of root system variation for shoot system morphology and suggests future exploration of rootstock genotypic diversity might offer a novel source of variation for shoot system phenotypic manipulation. 

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Allison Miller, Mani Awale, Anne Fennell, Zach Harris, Laszlo Kovacs, Misha Kwasniewski, Jason Londo, Zoe Migicovksy, Brigette Williams

Saint Louis University and the Danforth Plant Science Center; University of Missouri; South Dakota State University; Taylor Geospatial Institute; Missouri State University; Penn State University

Keywords

grafting, phenotyping, transcriptomics, epigenomics, ionomics 

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Acceptability of canned wines: effect of the level of involvement of consumers and type of wine

In recent years there has been a growing demand for alternative packaging designs in the food industry focused on diminishing the carbon footprint. Despite the environmental advantages of cans versus bottles, the traditional environment of wine has hindered the establishment of less contaminant containers. In this context, the objective of this study was to understand and generate knowledge about consumers´ perception of canned wines in comparison to bottled wines.

Assessing reserve nitrogen at dormancy for predicting spring nitrogen status in Chardonnay grapevines

Nitrogen (N) supply strongly influences vine productivity and berry composition, matching availability and uptake requirements of vines during the growing season is essential to optimize vine nutrition. The nutritional status of grapevines is commonly assessed by the determination of petiole nutrient concentrations at flowering. The reserve N could also be an earlier indicator for grapevine N status, this work aimed to assess how the petiole levels relate to these perennial N reserves.

OPTIMIZATION OF EXTRACTION AND DEVELOPMENT OF AN LC-HRMS METHOD TO QUANTIFY GLUTATHIONE IN WHITE WINE LEES AND YEAST DERIVATIVES

Glutathione is a natural tripeptide composed of l-glutamate, l-cysteine and glycine, found in various foods and beverages. In particular, glutathione can be found in its reduced (GSH) or oxidized form (GSSG) in must, wine or yeasts¹. Numerous studies have highlighted the importance of GSH in wine quality and aging potential². During winemaking, especially during aging on lees, GSH helps prevent the harmful effects of oxidation on the aroma of the wine³. Nevertheless, the amounts of GSH/GSSG present in wine lees are often unknown and the choice of operating conditions (quantity of lees and aging time) remains empirical.

Evaluation of methods used for the isolation and characterization of grape skin and seed, and wine tannins

Validation of the phloroglucinolysis and RP-HPLC method showed selectivity and repeatability within acceptable limits for all investigated matrices. Recovery of polymeric phenols by SPE was also acceptable.

Enological, economical, social and viticulture ”terroir” units as fundamental elements of mosaic of “big” zoning

Nous savons tous très bien qu’on a assisté au cours de ces dix dernières années à une éclosion soudaine de recherches sur le zonage viti-vinicole qui, à partir par exemple du modèle du concept de “terroir”, se sont de plus en plus enrichies en passant aux “Unités ou Systèmes de Transformation” (UTTE) et “Valorisation” (UTCE) pour terminer avec les “Systèmes productifs globaux du Territoire” (UTB) comprenant en filière les aspects existentiels (UTBES), sociaux (UTBSO) et économiques (UTBEC) hypothisés dans le “GRANDE ZONAZIONE: Grand zonage” (MORLAT R., 1996, CARBONNEAU A., 1996, TOUZARD J.M. 1998, CARBONNEAU A., CARGNELLO G., 1996, 1998, CARGNELLO G., 1994, 1995, 1996, 1998, 1999, 2001, -MILOTIC A., CARGNELLO G., PERSURIC G., 1999, PERSURIC G., STAYER M., CARGNELLO G., 2000, MILOTIC A., OPLANIC M., CARGNELLO G., PERSURIC G., 2000).