Rootstock x environment interaction shapes shoot system phenotypic variation in grafted ‘Chambourcin’

Wine lees are quantitatively the second most important wine by-product after grape stems and marc [1]. In order to recycle, distilleries recovered ethanol and tartaric acid contained in wine lees but yeast biomass is often unused. It has already been demonstrated that this yeast biomass could be upcycled to produce yeast extracts of interest for wine chemical stabilization [2]. In addition, it is well known that lees, during aging, release compounds that preserve wine from oxidation.

According to OIV reports, annual world wine consumption fluctuated around 240-245 mln hL over the past decade. The general market globalization has led to the situation when almost half of the consumed wine is exported to other countries. Of this volume, more than 60 mln hL are bottled still and sparkling wines.

In recent years, wine consumption has been evolving towards new trends. On the one hand, awareness of health and responsible consumption has been growing, and with it, the demand for wines with lower or without alcohol content [1].

In many regions, climate change leads to an increase in air temperature combined with a reduction of rainfall, intensifying climatic demand and water deficits (WD) (Cardell et al. 2019), which in turn may negatively impact grapevine development, yield and grape composition (Santos et al. 2020). In addition, climate change may also increase disease pressure, leading to further yield and quality losses, besides increasing costs due to increased vineyard spraying (Santos et al. 2020) and reducing viticulture acceptability by consumers (Guichard et al. 2017). Adopting new resistant varieties appears as a promising long-term solution to better manage vine protection, but unfortunately little is known regarding their behavior in front of WD.

Addressing the opportunities and challenges of genomics methods in grapevine (Vitis vinifera L.) requires the development of a comprehensive and accurate reference genome and annotation. We aimed to create a new gene annotation for the PN40024 grapevine reference genome by integrating the highly accurate and complete T2T assembly and the manually curated PN40024.v4 annotation. Here, we present a novel workflow to enhance the annotation of the T2T genome by incorporating past community input found in PN40024.v4. The pipeline’s containerization will improve the workflow’s reproducibility and flexibility, facilitating its inclusion as a shared workflow on the Grapedia portal, the grapevine genomics encyclopedia.