terclim by ICS banner
IVES 9 IVES Conference Series 9 Innovative approaches for fungicide resistance monitoring in precision management of grapevine downy mildew

Innovative approaches for fungicide resistance monitoring in precision management of grapevine downy mildew

Abstract

Effective control with fungicides is essential to protect grapevine from downy mildew, a devastating disease caused by the oomycete Plasmopara viticola. Managing this disease faces challenges in maintaining fungicide efficacy as the number of modes of action decreases and the risk of fungicide resistance increases. Long-term measures should address strains resistant to multiple modes of action, that can be selected by the repeated use of single-site fungicides. For these reasons, a precision management of the disease, that considers the selection of the best fungicide schedule according to the sensitivity profile of the pathogen population, is needed. Traditional techniques for fungicide sensitivity monitoring usually provide a qualitative information on fungicide resistance, limited presence/absence, and lack the resolution for high-throughput quantification and isolation of resistant individuals within pathogen populations. To overcome this limitation, an automated and high-throughput approach, based on flow cytometry and fluorescence-activated cell sorting, was developed on field sporangia populations. This method enables the identification and selection of single, live sporangia from a heterogenous field spore suspension, followed by inoculation on leaf discs treated with the selected fungicides. The resistant individuals, identified by the ability of growing at discriminatory rates of the fungicides or by dose-response analysis, are then quantified and isolated in a single assay, marking a significant advancement in sensitivity monitoring. The development of an antiresistance strategy, based on the sensitivity profile of the population, aims to preserve efficacy across all modes of action and achieve optimal disease control.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Beatrice Lecchi*, Giuliana Maddalena, Mattia Peracchi, Filippo Fanchi, Silvia Laura Toffolatti

Università degli Studi di Milano, Dipartimento di Scienze Agrarie e Ambientali – DISAA, Via Celoria 2, 20133 Milano

Contact the author*

Keywords

plant disease, integrated pest management, disease control, fungicide resistance, Plasmopara viticola

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Which risk assessment of water quality in pdo vineyards in Burgundy (France)?

To meet the demand of assessment tool of water managers we adapted to the vine production the INDIGO® method to developed initially for arable farming at the field scale.

Understanding sweetness of dry wines: first evidence of astilbin isomers in red wines and quantitation in a one-century range of vintages

The gustatory balance of wines relies on sweetness, bitterness and sourness. In dry wines, sweetness does not result from the presence of residual sugar as in sweet wines, but is due to other non-volatile compounds. Such taste-active compounds are released during winemaking, by grapes, yeasts or oak wood and belong numerous chemical families [1]. Beyond this diversity, stereochemistry of molecules can also influence their sensory properties [2]. However, the molecular determinants associated with this taste have only been partially elucidated. Astilbin (2R, 3R) was recently reported to contribute to wine sweetness [3]. As its aglycon contains two stereogenic centers, three other stereoisomers may be present: neoisoastilbin (2S, 3R), isoastilbin (2R, 3S), and neoastilbin (2S, 3S). These compounds have already been observed in natural products, but never in wine. This work aimed at assaying their presence for the first time in wines as well as their taste properties.The isomers were synthesized from astilbin and purified by semi-preparative HPLC.

Swiss terroirs studies

A multidisciplinary approach aiming at studying the grape-growing areas also referred as “Terroir” was initiated a few years ago in Switzerland.

Alimentary film to reduce cork taint and improve wine organoleptic quality

Wine quality may be compromised by mouldy off‒flavours related to cork taint. Although different compounds are considered to be involved in this wine defect, haloanisoles (HAs), and among them the 2,4,6-trichloroanisole (TCA), are claimed as the main responsible.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.