terclim by ICS banner
IVES 9 IVES Conference Series 9 Implementing VIS-NIR spectroscopy as a rapid and non-intrusive technique for assessing anthocyanin and phenolic concentrations in Vitis vinifera L. Grenache whole grape berries

Implementing VIS-NIR spectroscopy as a rapid and non-intrusive technique for assessing anthocyanin and phenolic concentrations in Vitis vinifera L. Grenache whole grape berries

Abstract

Anthocyanins and phenolic compounds play a crucial role in winemaking, contributing to the profile, flavor, color, texture, and stability of wine. Grape clusters, specifically Vitis vinifera L. cv. Grenache, were handpicked from a commercial vineyard sited in Tudelilla, La Rioja, Spain (42°18′ 52.26″, Long. -2°7′ 59.15″, Alt. 582 m) on five distinct dates from veraison to harvest during the 2015 season. Non-contact spectral measurements were conducted on intact grape berries using a VIS-NIR spectrometer operating in the 570 – 1000 nm spectral range under controlled laboratory conditions, positioned at a distance of 25 cm from the berries. The quantification of 16 anthocyanins and phenols in 120 grape clusters was performed using HPLC, established as the reference method for validating the spectral tool. Data exploration and prediction of phenolic concentration in grape berries were conducted through Principal Component Analysis (PCA) and Modified Partial Least Squares (MPLS) regression. The best calibration and cross-validation models were built for total monomeric anthocyanins, nonacylated anthocyanins and cyanidin 3-glucoside with determination coefficients (R2cv values above 0.86, while the standard errors of cross validation (SECV) were 0.058 mg/g, 0.052 mg/g and 0.001 mg/g respectively. Of the other phenolic groups, the model for total flavanol yielded R2cv = 0.66 and SECV = 0.023 mg/g. This technology shows high potential for the selection and classification of berries throughout ripening in the vineyard or upon grape reception at the winery. Its application could help tailoring the oenological fate of grape berries to various wine qualities or styles.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Juan Fernández-Novales1,2, Ignacio Barrio1,2, Leticia Martínez-Lapuente1,2, Zenaida Guadalupe1,2; María Paz Diago,1,2*

1 Department of Agriculture and Food Science. University of La Rioja. C/Madre de Dios 53. 26007. Logroño, (La Rioja) Spain
2 Institute of Sciences of Vine and Wine (CSIC, University of La Rioja, La Rioja Government) Finca La Grajera. Ctra. de Burgos Km 6. 26007. Logroño. (La Rioja). Spain

Contact the author*

Keywords

Berry ripening, Non-invasive technologies, Anthocyanin, Phenols, Chemometrics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Comparing different vineyard sampling densities and patterns for spatial interpolation of intrinsic water use efficiency

The need to rationalize agricultural inputs has recently increased interest in assessing vineyard variability in order to implement variable rate input applications, so-called ‘precision viticulture’. In many viticultural areas globally, precision viticulture is already widely used such as for selective harvesting and variable rate application (VRA) of inputs such as irrigation and/or fertilizer. Robust VRA relies on having a geostatistically accurate map (of one or more vineyard attributes) requiring high sampling densities, which can be cost- and time-prohibitive to obtain. Previous work on spatial interpolation using kriging have upscaled ground-based measurements, but such upscaling strategies are applicable only when vineyard conditions are spatially continuous and satisfies the assumption of second-order stationary processes. Alternatively, mixed models that combine kriging and auxiliary information, such as the regression kriging (RK) method, are more instructive for spatial predictions. In order to improve prediction accuracies, it is therefore necessary to incorporate additional information to achieve accurate spatial patterns with low error.

Pharmacological basis of the J-shaped curve in biological effects of wine

The classical pharmacological model assumes that the effect of a drug is proportional to the fraction of receptors occupied by the drug. In the simplest circumstances, the relationship between dose of a drug and response, when plotted on a logarithmic scale for drug concentration, is described by a sigmoidal curve. It presumes the existence of a threshold dose, below which no biological effect appears, and a maximal response in the form of a plateau, when a further increase in the dose of drug has no effect.

A synthesis approach on the impact of elevated CO2 on berry physiology and yield of Vitis vinifera

Besides the increase in global mean temperature the second main challenge of a changing climate is the increase in atmospheric carbon dioxide (CO2) in relation to physiology and yield performance of grapevines. The benefits of increasing CO2 levels under greenhouse environment or open field studies have been well investigated for various annual crops. Research under free carbon dioxide enrichment on field-grown perennial plants such as grapevines is limited to a few studies. Further, chamber and greenhouse experiments have been conducted mostly on potted vines under eCO2 conditions.

Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role. Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application. We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.

An Ag+ SPE method combined with Deans’ switch heart-cutting MDGC–MS/Olfactometry approach for identifying unknown volatile thiols in wine

Wine aroma is a crucial quality criterion. A multitude of volatile compounds have been identified and correlated to the aroma attributes perceived in wine.