terclim by ICS banner
IVES 9 IVES Conference Series 9 Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Abstract

The crunchy texture of table grapes is one of the key quality parameters during production. This varies from cultivar to cultivar, stage of harvest and vineyard performance. Cell wall properties are key drivers of berry quality (e.g., pericarp firmness and intactness) at harvest and beyond. Common practise amongst producers is to continuously monitor firmness by evaluating pericarp appearance of cross-sectioned berries prior to harvest. These qualitative methods can be quite arbitrary and imprecise in their execution, but more quantitative, yet simple and high-throughput methods to evaluate these cell wall polymers are not yet readily available. A promising avenue is to link carbohydrate arrays targeting cell wall polymers with more traditional biochemical methods with rapid infrared spectroscopy tools to ‘chemotype’ the cell walls of cultivars at specific stages of development (ripeness). We have integrated immunochemical datasets from over 15 different cultivars, such as ‘Crimson Seedless’ and ‘Prime’ which are well-known: with less well characterised cultivars such as ‘Autumn Crisp’ and ‘Sugar Crisp’ offering a means to ‘snapshot’ or ‘fingerprint’ the cell wall chemotype using spectroscopic methods. The ultimate aim would be to both provide new knowledge on berry cell walls of important cultivars as well as progressing the potential development of infrared sensing technology for predicting table grape cell wall quality (predicting if grapes will progress to soft or firm berries). The datasets and predictive models produced from this survey will be correlated with firmness and textural analysis performed on grape berries from different varieties and stages of ripeness.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

John P. Moore1*, Miguel Henriques1, Eugene Badenhorst1, Bodil JØrgensen2, Talitha Venter1

1 South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, South Africa
2 Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark

Contact the author*

Keywords

Cell walls, ripeness, berry firmness, spectroscopy, cultivars

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

L’étude “terroirs d’Anjou”: un exemple de caractérisation intégrée des terroirs viticoles, utilisable à l’échelle parcellaire

Natural factors of the production (“terroir” and vintage) are known as an important element for identifying wines by their genuine typicité and their authenticity. The program “Terroirs d’Anjou” (1994-1999) aims at bringing the necessary scientific basis for a rational and reasoned exploitation of the terroir.

Challenges and opportunities for increasing organic carbon in vineyard soils: perspectives of extension specialists

Increasing soil organic carbon (SOC) in vineyards enhances soil health with associated benefits for climate change resilience and mitigation.

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes.

Coping with extreme climatic events: some lessons from recent work on grapevine under heat peak

Climate change critically challenges viticulture. Among other threats, extreme and increasingly frequent heatwaves cause irreversible burns on leaves and bunches. A series of observations and experiments was conducted to better understand how leaf burns originate and whether genetics or management practices can mitigate them. In 2019, a panel of 279 potted cultivars of Vitis vinifera L. grown outdoors suffered a heat peak and a genetic origin of leaf burn variability was demonstrated. To deeper explore this variability, fourteen cultivars were selected for their contrasting responses to high temperatures, and detached leaves were submitted to a controlled increase in temperature up to 50 °C in a growth chamber.

Scientific research for an «Ad Maiora 4.1C» application «A step back towards the future universally sustainable EME4.1C». A concrete example of forward-looking and revolutionary entrepreneurial choices in the vine and wine sector

In 1979 an enlightened and farsighted business owner in an area and in an activity unknown to him and in 120 hectares of land cultivated with corn and wheat expressed to one of us that he wanted to start a business in the wine sector. The first innovative “Vigna Dogarina Scientific Applicative Project” has become famous and harmoniously inserted in and with the “Territoir” of eastern Veneto in northeastern Italy. The revolutionary project allowed one of us: 1. to put into practice results of research related to the applied philosophy, vision, methodology of the “Great MetaEthic Chain 4.1C®” algorithm of the “Conegliano Campus 5.1C®” that considers all material, immaterial, spiritual, technical, economic, environmental, social, existential, relational, ethical, MetaEthical factors with basic indexing in a harmonious chain “ 4.1C®” and application “5.1C®”, 2. to implement: