terclim by ICS banner
IVES 9 IVES Conference Series 9 Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Abstract

The crunchy texture of table grapes is one of the key quality parameters during production. This varies from cultivar to cultivar, stage of harvest and vineyard performance. Cell wall properties are key drivers of berry quality (e.g., pericarp firmness and intactness) at harvest and beyond. Common practise amongst producers is to continuously monitor firmness by evaluating pericarp appearance of cross-sectioned berries prior to harvest. These qualitative methods can be quite arbitrary and imprecise in their execution, but more quantitative, yet simple and high-throughput methods to evaluate these cell wall polymers are not yet readily available. A promising avenue is to link carbohydrate arrays targeting cell wall polymers with more traditional biochemical methods with rapid infrared spectroscopy tools to ‘chemotype’ the cell walls of cultivars at specific stages of development (ripeness). We have integrated immunochemical datasets from over 15 different cultivars, such as ‘Crimson Seedless’ and ‘Prime’ which are well-known: with less well characterised cultivars such as ‘Autumn Crisp’ and ‘Sugar Crisp’ offering a means to ‘snapshot’ or ‘fingerprint’ the cell wall chemotype using spectroscopic methods. The ultimate aim would be to both provide new knowledge on berry cell walls of important cultivars as well as progressing the potential development of infrared sensing technology for predicting table grape cell wall quality (predicting if grapes will progress to soft or firm berries). The datasets and predictive models produced from this survey will be correlated with firmness and textural analysis performed on grape berries from different varieties and stages of ripeness.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

John P. Moore1*, Miguel Henriques1, Eugene Badenhorst1, Bodil JØrgensen2, Talitha Venter1

1 South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, South Africa
2 Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark

Contact the author*

Keywords

Cell walls, ripeness, berry firmness, spectroscopy, cultivars

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Determination of the maturity status of white grape berries (Vitis vinifera L. cv Chenin) through physical measurements

La véraison, stade intermédiaire du développement de la baie de raisin, correspond au début de la maturation. Aux modifications de coloration de la pellicule sont associées une perte de fermeté, une diminution de l’acidité et une augmentation des teneurs en sucres et pigments ainsi que du volume de la baie. Le stade de véraison des cépages blancs reste difficile à apprécier visuellement. Son évaluation par palpation est subjective et donc sujette à caution.

Read More

A.O.C. taureau de Camargue

A.O.C. réservée aux viandes fraîches de bovins mâles ou femelles, nés, élevés et abattus dans une aire géographique définie (voir carte)

Read More

Reconnaissance des vins de terroir par les consommateurs

Approaching the notion of terroir wines at the level of consumption poses a problem due to the absence of a regulatory definition of the term terroir, which is not taken up either at Community level or at national level (the Consumer Code in particular does not define not the land). However, whatever definition is adopted for the terroir, we can retain at the consumer level an identification of the terroir through the different geographical mentions appearing on the labels or on the shelves of the wine shelf.

Read More

The role and quantification of vitamins in wine: what do we know?

AIM: Vitamins are essential compounds to numerous organisms, including yeasts, and appear highly significant during winemaking processes.

Read More

Is early defoliation a sustainable management practice for mediterranean vineyards? Case studies at the portuguese lisbon winegrowing region

Context and purpose of the study ‐ Recently early defoliation (ED) has been tested in several high‐ yielding grapevine varieties and sites aiming at reducing cluster compactness and hence, regulating yield and susceptibility to botrytis bunch rot infection. The reported results have been generally positive, encouraging growers to use this canopy management technique as an alternative for replacing the conventional time‐consuming cluster thinning and, simultaneously, as a sustainable practice to reduce the use of fungicides. However, ED increases berry sunburn risks and/or can induce carry‐over effects on vigor and node fruitfulness as shown in the two case studies reported in this work.

Read More