terclim by ICS banner
IVES 9 IVES Conference Series 9 Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Abstract

The crunchy texture of table grapes is one of the key quality parameters during production. This varies from cultivar to cultivar, stage of harvest and vineyard performance. Cell wall properties are key drivers of berry quality (e.g., pericarp firmness and intactness) at harvest and beyond. Common practise amongst producers is to continuously monitor firmness by evaluating pericarp appearance of cross-sectioned berries prior to harvest. These qualitative methods can be quite arbitrary and imprecise in their execution, but more quantitative, yet simple and high-throughput methods to evaluate these cell wall polymers are not yet readily available. A promising avenue is to link carbohydrate arrays targeting cell wall polymers with more traditional biochemical methods with rapid infrared spectroscopy tools to ‘chemotype’ the cell walls of cultivars at specific stages of development (ripeness). We have integrated immunochemical datasets from over 15 different cultivars, such as ‘Crimson Seedless’ and ‘Prime’ which are well-known: with less well characterised cultivars such as ‘Autumn Crisp’ and ‘Sugar Crisp’ offering a means to ‘snapshot’ or ‘fingerprint’ the cell wall chemotype using spectroscopic methods. The ultimate aim would be to both provide new knowledge on berry cell walls of important cultivars as well as progressing the potential development of infrared sensing technology for predicting table grape cell wall quality (predicting if grapes will progress to soft or firm berries). The datasets and predictive models produced from this survey will be correlated with firmness and textural analysis performed on grape berries from different varieties and stages of ripeness.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

John P. Moore1*, Miguel Henriques1, Eugene Badenhorst1, Bodil JØrgensen2, Talitha Venter1

1 South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, South Africa
2 Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark

Contact the author*

Keywords

Cell walls, ripeness, berry firmness, spectroscopy, cultivars

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Variety “Rebula” (Vitis vinifera L.) determines the terroir Goriška brda “Collio” in Slovenia

A «terroir» is a group of vineyards from the same region, belonging to a specific appellation, and sharing the same type of soil, weather conditions, grapes and wine making savoir-faire, which contribute its specific personality to the wine. White wine variety «Rebula» or «Ribolla gialla» is a local and traditional variety, which is mentioned already in XIII. century like variety for tax paying and merchandise.

Antimicrobial activity of oenological polyphenols against Gram positive and Gram negative intestinal multidrug-resistant bacteria

Bacterial antibiotic resistance is a major current health problem. Polyphenols have demonstrated antibacterial activity, and in this work we studied the effect of oenological polyphenols on the growth of intestinal multidrug-resistant strains of human and animal origin. Two Enterococcus faecium strains, resistant to vancomycin and other antibiotics, and four Escherichia coli strains, resistant to ampicillin and other antibiotics, were included in this study. All strains showed multidrug resistant phenotypes and genotypes to at least two antibiotic families.

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

A 4D high resolution vineyard soil assessment for soil-hydrological interpretation in combination with automated data analysis and visualization to manage site-specific grape and wine quality

A Visual Information eNvironment for Effective agricultural management and Sustainability (VINES) is under development, which can provide significant competitive advantages to winegrowers by sustaining their appellation-specific grape and wine qualities and yields while measurably conserving water resources.

Different oxygen and sulphur dioxide concentrations in ‘Sauvignon blanc’ must: effect on the composition of the must and wine

The effects of different oxygen and sulphur dioxide additions to South African ‘Sauvignon blanc’ musts were investigated. Oxygen addition without SO2 protection led to lower levels of certain volatile thiols in the wines, with a corresponding decrease in certain phenols and glutathione concentrations.