terclim by ICS banner
IVES 9 IVES Conference Series 9 Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Screening table grape cultivars using cell wall ELISA and glycan microarrays for berry firmness and quality parameters

Abstract

The crunchy texture of table grapes is one of the key quality parameters during production. This varies from cultivar to cultivar, stage of harvest and vineyard performance. Cell wall properties are key drivers of berry quality (e.g., pericarp firmness and intactness) at harvest and beyond. Common practise amongst producers is to continuously monitor firmness by evaluating pericarp appearance of cross-sectioned berries prior to harvest. These qualitative methods can be quite arbitrary and imprecise in their execution, but more quantitative, yet simple and high-throughput methods to evaluate these cell wall polymers are not yet readily available. A promising avenue is to link carbohydrate arrays targeting cell wall polymers with more traditional biochemical methods with rapid infrared spectroscopy tools to ‘chemotype’ the cell walls of cultivars at specific stages of development (ripeness). We have integrated immunochemical datasets from over 15 different cultivars, such as ‘Crimson Seedless’ and ‘Prime’ which are well-known: with less well characterised cultivars such as ‘Autumn Crisp’ and ‘Sugar Crisp’ offering a means to ‘snapshot’ or ‘fingerprint’ the cell wall chemotype using spectroscopic methods. The ultimate aim would be to both provide new knowledge on berry cell walls of important cultivars as well as progressing the potential development of infrared sensing technology for predicting table grape cell wall quality (predicting if grapes will progress to soft or firm berries). The datasets and predictive models produced from this survey will be correlated with firmness and textural analysis performed on grape berries from different varieties and stages of ripeness.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

John P. Moore1*, Miguel Henriques1, Eugene Badenhorst1, Bodil JØrgensen2, Talitha Venter1

1 South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, South Africa
2 Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark

Contact the author*

Keywords

Cell walls, ripeness, berry firmness, spectroscopy, cultivars

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Outline for the définition of “Terroirs Viticoles application to the area of El AIjarafe (Seville, Spain)

The grapes producing and wine making regions are différent in their use of agricultural, industrial or agroindustrial means. These means are quite often very original and/or specialised; and lately are also quite competitive. Such means are being defined with increased accuracy in the delimitation and definition of its characteristics (Paneque et al., 1996 a). Human action together with other Elements and Agents involved in the vine growing production (Reyner, 1989) over these means lead to agronomic systems with important characteristics. Finally, the transformation of the vine growing production, through different technologies (Fleet, 1992), results in the creation of products with a different acceptance and economical value in the market.

Vine nitrogen status and the terroir effect: a study on cv. Doral in the Vaud vineyard (Switzerland)

A 3-year study was conducted in the Vaud vineyard (Switzerland) to evaluate the effects of « terroir » on the ecophysiology and fruit compostion of Vitis vinifera L. cv.

Identification and quantification of molecular ellagitannins in cognac eaux-de-vie by a mass spectrometry method: barrel toasting and aging impact

Ellagitannins are the main oak wood phenolic compounds that contribute to wine and spirits organoleptic quality (color, astringency, bitterness)(1-3). Given the lack of knowledge regarding their composition and evolution in spirits, the objectives were to follow their extraction kinetic in Cognac “eaux-de-vie” matured in barrel representing different toasting and to observe their evolution and structural modifications during aging. METHODS: Eight different toasting levels were used for studying the impact of the toasting on ellagitannins composition. Two verticals (1978-2018) of “eaux-de-vie” samples coming from two terroirs were analyzed in order to observe ellagitannins evolution during aging. The above analyses were conducted using HPLC-Triple Quadrupole mass spectrometer (4) and the unknown compounds were identified by UPLC-Q-TOF, purified by preparative HPLC prior to 1D/2D-NMR analysis.

Come proteggere un territorio viticolo: il punto di vista del giurista

La valanga di fango che si è abbattuta nel Salemitano e nell’Avellinese, provocando decine di vittime, è stata causata in larga misura dalle insufficienti opere idrauliche e dalla manca­ta manutenzione di antiquati canali idrici.

Organic mulches slightly influence wine phenolic composition and sensorial properties

Grapevines have traditionally been grown in semi-arid areas, but viticulture is now compromised by climate change. Therefore, it is necessary to implement environmentally friendly viticulture practices to adapt grapevines to current climatic conditions. In this context, organic mulches offer many benefits, such as reduced soil erosion and increased organic matter, soil water content and crop productivity. However, these practices must not compromise grape and wine quality. Therefore, the objective of this study was to evaluate the effect on wine physicochemical and phenolic composition and sensorial properties of different soil management practices on the vine row. Over four years, five soil treatments were examined in two different vineyards.