OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Flor yeast diversity and dynamics in biologically aged wines

Flor yeast diversity and dynamics in biologically aged wines

Abstract

Wine biological aging is characterized by the development of yeast strains that form a biofilm on the wine surface after alcoholic fermentation. These yeasts, known as flor yeasts, form a velum that protects the wine from oxidation during aging. Thirty-nine velums aged from 1 to 6 years were sampled from “Vin jaune” from two different cellars. 

We show for the first time that these velums possess various aspects in term of color and surface aspects. Surprisingly, the heterogeneous velums are mostly composed of one species, S. cerevisiae. Scanning electron microscope observations of these velums revealed unprecedented biofilm structures and various yeast morphologies formed by the sole S. cerevisiae species. Our results highlight that different strains of Saccharomyces are present in these velums. Unexpectedly, in the same velum, flor yeast strain succession occurred during aging, supporting the assumption that environmental changes are responsible for these shifts. Despite numerous sample wine analyses, very few flor yeasts could be isolated from wine following alcoholic fermentation, suggesting that flor yeast development results from the colonization of yeast present in the aging cellar. We analyzed the FLO11 and ICR1 sequence of different S. cerevisiae strains in order to understand how the same strain of S. cerevisiae could form various types of biofilm. Among the strains analyzed, some were heterozygote at the FLO11 locus, while others presented two different alleles of ICR1 (wild type and a 111 bp deletion). We could not find a strong link between strain genotypes and velum characteristics. The same strain in different wines could form a velum having very different characteristics, highlighting a matrix effect.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Hervé Alexandre, Vanessa David-Vaizant 

AgroSup Dijon, PAM UMR A 02.102, Université Bourgogne Franche-Comté, Dijon, France, 2 Equipe VAlMiS, Institut Universitaire de la Vigne et du Vin, Dijon, France 

Contact the author

Keywords

flor yeast, FLO11, Saccharomyces cerevisiae, Vin Jaune

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

A climatic characterisation of the sub-Appellations in the Niagara Peninsula wine region

This study used climatic and topographic data to characterize the sub-appellations that have been recently delineated in the Niagara Peninsula viticulture area in order to assess their potential for ripening early to late season Vitis vinifera varieties. No major differences were found in the ripening-period mean temperatures, but major differences in the diurnal temperature ranges were observed.

Managing precision irrigation in vineyards: hydraulic and molecular signaling in eight grapevine varieties

Understanding the physiological and molecular bases of grapevine responses to mild to moderate water deficits is fundamental to optimize vineyard irrigation management and identify the most suitable varieties. In Mediterranean regions, the higher frequency of heat waves and droughts highlights the importance of precision irrigation to meet vine water demands and demonstrates the necessity for a deeper understanding of the different physiological responses among varieties under water stress. In this context, previous reports show an interplay between stomatal regulation of transpiration and changes in leaf hydraulic conductivity, also with the involvement of aquaporins (AQPs), particularly under water stress. However, how those signaling mechanisms are regulated in different grapevine varieties along phenological phases is unclear.

Use of membrane ultrafiltration technology to achieve protein stabilisation of white wine

AIM: Proteins in white wine can cause cloudiness or haze after bottling, which consumers may consider an indicator of poor quality. . As a consequence, winemakers often use bentonite, a clay-based material that binds protein, to remove proteins and achieve protein stabilisation. However, removing bentonite from wine after treatment can result in a 3-10% loss of wine (1)…

Isohydric and anisohydric behavior of 18 wine grape varieties grown in an arid climate

The interest in understanding the water balance of terrestrial plants under drought has led to the creation of the isohydric/anisohydric terminology. The classification was related to an implication-driven framework, where isohydric plants maintain a constant and high leaf water potential through an early and intense closure of their stomata, hence risking carbon starvation. In contrast, anisohydric plants drop their leaf water potential to low values as soil drought is establishing due to insensitive stomata and thus risk mortality through hydraulic failure, albeit maximizing carbon intake. When applied to grapevines, this framework has been elusive, yielding discrepancies in the classification of different wine grape varieties around the world.

BIOSORPTION OF UNDESIRABLE COMPONENTS FROM WINE BY YEAST-DERIVED PRODUCTS

4-Ethylphenol (EP) in wine is associated with organoleptic defects such as barn and horse sweat odors. The origin of EP is the bioconversion reaction of p-coumaric acid (CA), naturally present in grapes and grape musts by contaminating yeasts of the genus Brettanomyces bruxellensis.
Yeast cell walls (YCW) have shown adsorption capacities for different compounds. They could be applied to wines in order to adsorb either CA and/or EP and thus reduce the organoleptic defects caused by the contaminating yeasts.