Yeast diversity in Vitis labrusca l. Ecosystems

Abstract

Although there are detailed studies on the microbiota of Vitis vinifera L. grapes, little is known about the diversity of yeast communities present in non-vinifera Vitis ecosystems (i.e., grapes and spontaneously fermenting grape musts). Potentially scientific and/or enological valuable yeast strains from these non-vinifera Vitis ecosystems might never be isolated from V. vinifera L. Using a standard culture-dependent strategy, we studied the population of yeast species during initial stages of spontaneous fermentation of V. labrusca L. (Isabella) grape musts. Rare non-Saccharomyces yeast species were recognized in Isabella, including Candida azymoides, Pichia cecembensis, Candida californica, Candida bentonensis, Issatchenkia hanoiensis and Candida apicola. Interestingly, P. cecembensis, not previously recognized in V. vinifera grapes or musts, was also found in V. labrusca L. grapes in Portugal (Azores Archipelago). Thus, this yeast species could be specifically associated with V. labrusca L. grapes, regardless of their geographic origin and/or the associated human interventions. Moreover, I. hanoiensis, a yeast species rarely isolated in V. vinifera grapes, was also identified in V. labrusca ecosystems from Argentina and Portugal. These results suggest that specific Vitis-microbial interactions may underlie the assembly of specific grape vine yeast communities. Also interestingly, some yeast genera commonly isolated from V. vinifera ecosystems (e.g., Hanseniaspora, Torulaspora and Metschnikowia) were rarely identified and almost never dominated the yeast communities in the V. labrusca L. musts we analyzed. Our results reinforce the research interest in biodiversity and extraordinary wine yeasts in ecological niches alternative to traditional V. vinifera ecosystems.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Alberto Luis Rosa, Maria LauraRaymond, Francisco Conti

Laboratorio de Genética y Biología Molecular IRNASUS – CONICET Facultad de Ciencias Quimicas – Universidad Catolica de Cordoba Cordoba – Argentina 

Contact the author

Keywords

Vitis, labrusca, yeast, biodiversity

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

2018 updates on the agronomic performances of fungus resistant wine grapes in Trentino (Italy)

On the market there are several wine grapes which are tolerant to the main fungal diseases. These varieties, commonly defined “resistant”, were developed in the grapevine breeding programs carried out mainly in Germany, France, Hungary and Italy. Some of these cultivars have been included in the national catalogues of wine grape varieties and have sometimes been allowed for specific kinds of wine. The VEVIR project, aimed at the enological evaluation of resistant vines, involves 33 cultivars achieved at the State Institute for Viticulture Freiburg in Germany, the Research Institute of Viticulture and Enology Pecs in Hungary and the Fondazione Edmund Mach S. Michele all’Adige (FEM) in Italy.

Distribution of fungicide-resistant Botrytis cinerea mutations in the Tokaj and Eger wine regions

Botrytis cinerea is one of the most widespread host-specific fungal pathogens, causing significant yield losses and economic damage to vineyards every year.

Does bioprotection by adding yeasts present antioxydant properties?

AIM: The bioprotection by adding yeasts is an emerging sulfur dioxide alternative. Sulfur dioxide is a chemical adjuvant used for its antiseptic, antioxidasic and antioxidant properties. Faced with the societal demand (Pérès et al., 2018) and considering the proven human risks associated with the total doses of sulfur dioxide (SO2) present in food requirements (García‐Gavín et al., 2012), the reduction of this chemical input is undeniable.

PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

Plants in their natural environment are in continuous interaction with large numbers of potentially pathogenic and beneficial microorganisms. Depending on the microbe, plants have evolved a variety of resistance mechanisms that can be constitutively expressed or induced. Phytoalexins, which are biocidal compounds of low to medium molecular weight synthesized by and accumulated in plants as a response to stress, take part in this intricate defense system.1,2
One of the limitations of our knowledge of phytoalexins is the difficulty of analyzing their spatial responsiveness occurring during plant- pathogen interactions under natural conditions.

Influence of grape withering on corvina and corvinone aroma composition

AIM:Valpolicella is a wine region located in Italy north-east, famous for the production of dry and sweet red wines from withered grapes, including Amarone and Recioto. The aim of this study is to understand the influence of the withering process on Corvina and Corvinone wines aroma profiles. METHODS:Wines were produced with a standard red wine winemaking protocol with Corvina and Corvinone grapes from different Valpolicella vineyards and vintages. In consideration of the local traditional practice of post-harvest withering of the grapes, wines from each vineyard were obtained from either fresh and withered grapes. Wines were analysed by Solid Phase Extraction and Solid Phase Micro Extraction gas chromatography coupled to mass spectrometry.