Yeast diversity in Vitis labrusca l. Ecosystems

Abstract

Although there are detailed studies on the microbiota of Vitis vinifera L. grapes, little is known about the diversity of yeast communities present in non-vinifera Vitis ecosystems (i.e., grapes and spontaneously fermenting grape musts). Potentially scientific and/or enological valuable yeast strains from these non-vinifera Vitis ecosystems might never be isolated from V. vinifera L. Using a standard culture-dependent strategy, we studied the population of yeast species during initial stages of spontaneous fermentation of V. labrusca L. (Isabella) grape musts. Rare non-Saccharomyces yeast species were recognized in Isabella, including Candida azymoides, Pichia cecembensis, Candida californica, Candida bentonensis, Issatchenkia hanoiensis and Candida apicola. Interestingly, P. cecembensis, not previously recognized in V. vinifera grapes or musts, was also found in V. labrusca L. grapes in Portugal (Azores Archipelago). Thus, this yeast species could be specifically associated with V. labrusca L. grapes, regardless of their geographic origin and/or the associated human interventions. Moreover, I. hanoiensis, a yeast species rarely isolated in V. vinifera grapes, was also identified in V. labrusca ecosystems from Argentina and Portugal. These results suggest that specific Vitis-microbial interactions may underlie the assembly of specific grape vine yeast communities. Also interestingly, some yeast genera commonly isolated from V. vinifera ecosystems (e.g., Hanseniaspora, Torulaspora and Metschnikowia) were rarely identified and almost never dominated the yeast communities in the V. labrusca L. musts we analyzed. Our results reinforce the research interest in biodiversity and extraordinary wine yeasts in ecological niches alternative to traditional V. vinifera ecosystems.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Alberto Luis Rosa, Maria LauraRaymond, Francisco Conti

Laboratorio de Genética y Biología Molecular IRNASUS – CONICET Facultad de Ciencias Quimicas – Universidad Catolica de Cordoba Cordoba – Argentina 

Contact the author

Keywords

Vitis, labrusca, yeast, biodiversity

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Which heat test really represents the haze risk of a white Sauvignon wine ?

AIM: Different heat tests are used to predict a white wine haze risk after bottling. The most used tests are 30-60 min. at 80°C. Nevertheless, there is a lack of information about the relationship between the wine haze observed after such tests and the turbidities observed in the bottles after the storage/transport of the wines in more realistic Summer conditions (35-46°C during 3-12 days)

Comparative study of qualitative and quantitative characters of grape cultivar ‘Mavrodafni’ (Vitis vinifera L.) grown in different regions of the PDO Mavrodafni Patras

‘Mavrodafni’ (Vitis vinifera L.) is considered one of the oldest grapevine cultivars indigenous to the Greek vineyard, with western Peloponnese being its primary center of cultivation. ‘Renio’ is considered to be either a variant of ‘Mavrodafni’ or an altogether different cultivar. Both ‘Mavrodafni’ and ‘Renio’ can be found in the vineyards of the centers of cultivation, since ‘Renio’ is considered to be more productive compared to ‘Mavrodafni’, and for this reason, it has gradually replaced ‘Mavrodafni’ from cultivation over the course of time. The aim of the present study was to assay the mechanical properties, the polyphenolic content and the antioxidant capacity of skin extracts and must of berries coming from ‘Mavrodafni’ and ‘Renio’, cultivated in the same vineyard as well as in the different regions of cultivation of the PDO Mavrodafni Patras.

PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

Plants in their natural environment are in continuous interaction with large numbers of potentially pathogenic and beneficial microorganisms. Depending on the microbe, plants have evolved a variety of resistance mechanisms that can be constitutively expressed or induced. Phytoalexins, which are biocidal compounds of low to medium molecular weight synthesized by and accumulated in plants as a response to stress, take part in this intricate defense system.1,2
One of the limitations of our knowledge of phytoalexins is the difficulty of analyzing their spatial responsiveness occurring during plant- pathogen interactions under natural conditions.

HYDROXYTYROSOL PRODUCTION BY DIFFERENT YEAST STRAINS: SACCHAROMYCES AND NON-SACCHAROMYCES AND THE RELATION WITH THE NITROGEN CONSUMPTION

Hydroxytyrosol (HT) is a phenolic compound with extensive bioactive properties. It is present in olives, olive oil and wines. Its occurrence in wines is partly due to yeast synthetise tyrosol from tyrosine by the Ehrlich pathway, which is subsequently hydroxylated to .
The aim of the present work is to study how different yeast strains can influence in the HT production and, how the different nitrogen consumption of each strain can interfere the production of bioactive compounds.

Comparison of the principal production methods for alcohol-free wine based on analytical parameters

Production, demand, and brand awareness of dealcoholized wine (<0.5% v/v) is steadily increasing worldwide. However, there have been few studies to date investigating and comparing the different physical processes for dealcoholizing wine.