Comparison of tannin analysis by protein precipitation and normal-phase HPLC

Umami is defined as a pleasant and savory taste derived from glutamate, inosinate and guanylate, which are naturally present in meat, fish, vegetables and dairy products. The term “kokumi” refers to a complex flavour sensation, characterized by thickness, fullness and continuity.

Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. This process of multiplication leads to spontaneous somatic mutations that can eventually generate a variant phenotype, of potential interest for cultivar improvement and innovation. However, regardless their phenotypic effect, somatic mutations stack in the genome, and they can be used to reveal the origin and dissemination history of ancient cultivars. Here, a stringent somatic variant calling over whole genome resequencing data from 35 ‘Tempranillo Tinto’ clones or old vines from seven Iberian winemaking regions revealed 135 single nucleotide variations (SNVs) shared by some of the clonal lines.

The utilization of non-Saccharomyces yeasts in the wine industry has increased significantly in recent years. Alternative species need commonly be employed in combination with Saccharomyces cerevisiae to avoid stuck fermentation, or microbial spoilage. The employment of more than one yeast starter can lead to interactions between different species with an impact on the outcome of wine fermentation. Previous studies[1] demonstrated that S. cerevisiae elicits transcriptional responses with both shared and species-specific features in co-culture with other yeast species.

The mechanisms behind thiol precursor accumulation in grapes remain incompletely understood, nor are the ways in which they can be improved by agronomic practices. A six-year field trial studied the physiological response of the Swiss white cultivar Vitis vinifera Arvine, rich in varietal thiols and precursors, to canopy management, i.e. leaf removal and canopy height.. Five treatments were set up in a randomized block design to assess the impacts of 1) pre-flowering LR (i.e. pre-flowering or full-flowering stages) and 2) compensating for the leaf area removed in the cluster zone by increasing the trimming height (i.e. 100 or 150 cm canopy height), compared with a non-defoliated control treatment.
Intensive pre-flowering LR severely reduced yield potential (–47% on average) and reduced the concentration of 3-mercaptohexanol precursors (P-3MH) in the must (–21%; p-value < 0.10).

Botrytis bunch rot occurrence is one of the most important limitations for the wine industry in humid environments. A positive correlation between grapevine growth and susceptibility to fungal pathogens has been found. In theory the effect of grapevine vegetative growth on bunch rot expression results from direct effects (cluster architecture, nitrogen status among others) and indirect ones (via microclimate). However, a reduction in bunch rot incidence can be achieved in some circumstances without major vine growth reduction. The present study was aimed to test the general hypothesis that bunch rot susceptibility is affected by vine vigor, but other factors associated with grapevine vegetative expression could be even more relevant.