OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analysis and composition of grapes, wines, wine spirits 9 Comparison of tannin analysis by protein precipitation and normal-phase HPLC

Comparison of tannin analysis by protein precipitation and normal-phase HPLC

Abstract

Tannins are a heterogenous class of polymeric phenolics found in grapes, oak barrels and wine. In red wine tannins are primarily responsible for astringency, though they also have an important role in reacting with and stabilizing pigments. There are numerous sub-classes of tannins found in wine but they all share structural heterogeneity within each sub-class, with varied polymer composition, configuration and length. 

Numerous methodologies exist for the quantification of tannins, however, protein precipitation using bovine serum albumin has proved itself useful due to its strong correlation to the sensory perception of astringency and the basic instruments required for the method. Though the method can yield valuable insights into tannin composition, it cannot be automated easily and necessitates well-trained personnel. 

RP-HPLC analysis has been used for the quantification of low molecular phenolic compounds for a long time, but it is not suitable for the quantification of tannins. A normal-phase (NP)-HPLC method using a ternary solvent system is suggested, which is able to separate the phenolic compounds from red wine into three major fractions. Comparison with standard phenolic compounds allowed the characterization and quantification of these fractions and the results were compared to those obtained by protein precipitation.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Jan-Peter Hensen, Ingrid Weilack, Fabian Weber, Andreas Schieber, James Harbertson

University of Bonn Institute of Nutritional and Food Sciences, Molecular Food Technology Endenicher Allee 19b D-53115 Bonn Germany 

Contact the author

Keywords

Tannin analysis, Protein Precipitation Assay, NP-HPLC

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Impact of copper residues in grape must on alcoholic fermentation: effects on yeast performance, acetaldehyde and SO2 production

A relevant trend in winemaking is to reduce the use of chemical compounds in both the vineyard and winery.

Use of satellite in precision viticulture: the Franciacorta experience

Today, the concept of precision vine management (or site-specific viticulture) has a great relevance. It is based on the practice of a different management in relation to the different features of the crop site. In this way, all practices should be adapted to the land spatial variability and should be linked to the real needs of vines.

Oxidation vs reduction: the fate of tannins, pigments, vscs, color,SO2 and metabolomic fingerprint

The management of oxygen during winemaking and aging is a big issue in order to achieve high quality wines. The correct amount of O2 improves aroma, astringency, bitterness and color, however an excess of oxygen promotes the appearance of yellow

Les terroirs viticoles ont une histoire

The historian starts from a scientific, rigorous and recent definition of the wine-growing region. “A viticultural terroir is made up of several homogeneous units: geological and pedological elements (texture,
grain size, thickness, mineralogical nature, chemical components), geomorphological (altitude, slope, exposure), climatological (rainfall, temperature, insolation)”. Absent from this definition, the man is fortunately reintroduced a little further. By associating viticulture and winemaking, it forms a “couple” with the terroir and this couple.

Influence of toasting oak wood on ellagitannin structures

Ellagitannins (ETs) have been reported to be the main phenolic compounds found in oak wood. These compounds, belonging to the hydrolysable tannin class of polyphenols, are esters of hexahydroxydiphenic acid (HHDP) and a polyol, usually glucose or quinic acid. They own their name to their capacity to be hydrolysed and liberate ellagic acid and they have an impact on astringency and bitterness sensation, which is strongly dependant on their structure. The toasting phase is particularly crucial in barrels fabrication and influences wood composition.