terclim by ICS banner
IVES 9 IVES Conference Series 9 Reduced bunch compactness in a clone of Tempranillo associates with a complex reciprocal translocation detected by long-read sequencing genomics

Reduced bunch compactness in a clone of Tempranillo associates with a complex reciprocal translocation detected by long-read sequencing genomics

Abstract

Grapevine cultivars are vegetatively propagated to maintain their varietal attributes. However, spontaneous somatic variation emerges during prolonged periods of vegetative growth, providing an opportunity for the natural improvement of traditional grapevine cultivars. Notably, reduction in bunch compactness is a favorable trait in viticulture, offering advantages such as decreased susceptibility to bunch fungal diseases, and a more uniform ripening of berries. To unravel the genetic and developmental mechanisms behind bunch compactness variation, we examined a somatic variant of Tempranillo Tinto cultivar with loose bunches. We found that the mutant clone exhibits a ~50% reduction in pollen viability compared to typical Tempranillo clones. By aligning Illumina and Nanopore whole-genome sequencing reads to a diploid genome assembly of Tempranillo, we identified genome structural variations (SV) specific of this clone: translocation events involving chromosomes 1-3, 7-11, and 8-17. The presence of the SV breakpoints was validated using PCR and Sanger sequencing. The analysis of self-cross progeny of the mutant clone showed that low pollen viability and reduced number of seeds per berry co-segregate with the SV event between specific haplotypes of chromosomes 1 and 3, suggesting a causal effect for this rearrangement. Inspection of Nanopore read alignments identified that the SV 1-3 event corresponds to a complex reciprocal translocation with duplications at the breakpoints of the two involved chromosomes. Considering that heterozygous reciprocal translocations associate with partially incompatible chromosome pairing during meiosis, we propose that this type of SV decreases fruit set rate by lowering gamete viability, ultimately reducing bunch compactness.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Pablo Carbonell-Bejerano1*, Noelia Alañón1, Yolanda Ferradás1,2, Nuria Mauri1,3, José Miguel Martínez-Zapater1, Javier Ibáñez1*

1 Instituto de Ciencias de la Vid y del Vino (ICVV, CSIC-CAR-UR), Departamento de Viticultura, Logroño, Spain
2 Departamento de Biología Funcional, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
3 Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Cerdanyola del Vallès, Barcelona, Spain

Contact the author*

Keywords

bunch compactness, clonal variation, genome structural variation, pollen viability, reciprocal translocation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.

Overcoming habit formation in the production of wine

Evidence indicates that climate change affects the environment, human health, and well-being via drought, increasing greenhouse effect, and climatic catastrophes. As the wine sector is also negatively affected by climate change, the role of climate change mitigation and adaptation policies is important in wine production. One example of an adaptation policy is the implementation of grapevine genetics (duchene, 2016), while organic farming may be used as an approach to mitigate the consequences of climate change (vinci et al., 2022). To this end, the european commission’s objective is to reach the european green deal target of at least 25% of the european union’s agricultural land under organic farming by 2030.

Landscape marketing and landscape reality: what is the relationship? The case of the Loire Valley vineyards

This issue poses two questions: the relationship between beauty and taste (is landscape quality an index of wine quality ?), and the gap or the conformity between our image of the “terroir” and the visible reality. The landscape is both an object and a representation.

Study of grape physiology and wine quality (cv. Merlot) in different identified terroirs of the canton Ticino (Switzerland)

Une étude de la physiologie de la vigne (cv. Merlot) et de la qualité des vins a été réalisée au Tessin de 2006 à 2008. La méthodologie utilisée pour cette étude intégrait tous les paramètres qui définissent les terroirs: facteurs naturels (géologie, pédologie et climat), facteurs physiologiques de la vigne et qualité des vins qui sont les révélateurs de la valeur d’un terroir.

Tannin potential and molecular toasting in cooperage: a tool to modulate fruity expression of red wine

AIM: Oak wood play traditionally a huge role in making fine red wines. During wine maturation, barrel yields some of its constituents to the wine and leads to the improvement of its quality, contributing to richness and complexity [1].