terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Abstract

Uruguay is known for the production of Tannat wines, which is a neutral variety from an aroma point of view, but capable of providing aromatic precursors that are of interest in the production of wines for ageing. The main aromatic precursors present are glycosidic compounds and carotenoids. The contribution of carotenoid degradation by-products such as norisoprenoids to wine aroma is fundamental, as they are associated with pleasant aroma descriptors and very low olfactory perception thresholds. Several factors have been shown to influence carotenoid concentrations in grapes, such as cultivar, climatic conditions, viticultural region, plant water status, exposure to sunlight and ripening stage. Norisoprenoids can be formed by chemical or enzymatic degradation of carotenoids. In this work, we studied two contrasting clones from the same plot as an experimental strategy to minimize the environmental factor and focus on the genes of interest. We evaluated glycosidic precursors (SPE-GC-MS), carotenoids (HPLC-DAD) and gene expression (RNA-Seq) in the selected clones during four stages of grape ripening. Significant differences in carotenoid and norisoprenoid content were found throughout the ripening period. Comparisons between clones showed significant differences in carotenoid content but not in norisoprenoid content during this harvest. Many genes associated with carotenoid and norisoprenoid biosynthesis showed differential expression throughout the ripening period in each clone. However, no genes were differentially expressed between clones. We conclude that the differences between clones do not manifest themselves every year. This shows that climate plays a fundamental role in aroma biosynthesis in Tannat clones.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Cecilia C. Da Silva1*, Nicolas Nieto2, Andres Coniberti3, Eduardo Boido2, Francisco Carrau2, Eduardo Dellacassa4, Laura Fariña2

1 PDU Espacio de Biología Vegetal del Noreste, sede Tacuarembó, CENUR Noreste, Universidad de la República, Tacuarembó, Uruguay
2 Área Enología y Biotecnología de Fermentaciones, CYTAL, Facultad de Química, Universidad de la República, Montevideo, Uruguay
3 Estación Experimental “Wilson Ferreira Aldunate”, Instituto Nacional de Investigación Agropecuaria, Canelones, Uruguay
4 Laboratorio de Biotecnología de Aromas, DQO, Facultad de Química, Universidad de la República, Montevideo, Uruguay

Contact the author*

Keywords

Carotenoid, Norisoprenoid, Tannat, GC-MS, RNA-Seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Ochratoxin A (OTA) is a mycotoxin produced by several filamentous fungi infecting grape bunches (Penicillium and Aspergillus spp.), this toxin pass to must when grapes are crushed and later it is found in wine. Following the evaluations of the toxicity of OTA, European Commission Regulations have been promulgated introducing upper limits for OTA concentrations in various commodities (cereals, cereal products, dried vine fruit, coffee, wine, grape juice, baby foods and dietary foods for special medical purposes).

Physical-chemical and sensory characterization of wine made with the cultivar syrah produced in a double pruning system

In recent years, the consumption of fine wines in Brazil has increased significantly, a phenomenon that is also reflected in the expansion of production to new regions. In the brazilian southeast for example, the so-called “winter wines” are being produced, through management in two cycles, one of formation and one of production, with two prunings and one harvest per year, a technique known as double pruning, with vineyards established at altitudes close to or above 1,000 m above sea level.

Il Lambrusco reggiano e il territorio di pianura: un modello efficace

Il caso “Lambrusco” è emblematico di un buon connubio tra un gruppo di vitigni ed un territorio di pianura caratterizzato da suoli fertili e alluvionali, che determinano un elevato sviluppo

Market entry strategies in the U.S. alcohol distribution: The case of French wine exporters

This study examines the different strategies adopted by wine exporters located in France for penetrating international alcohol distribution networks in the U.S. market (and to a lesser extent the Canadian market). Grounded in the Business-to-Business (B2B) marketing literature (Ellegaard and Medlin, 2018), this study adopts a framework integrating a ‘Stakeholder’ approach for understanding the logics behind exporters’ strategies to penetrate the alcohol distribution networks (wholesalers, importers, alcohol monopolies).

Techniques to study graft union formation in grapevine 

Grapevines are grown grafting in most viticultural regions. Grapevine rootstocks are either hybrids or pure species of different American Vitis spp. (particularly V. berlandieri, V. rupestris and V. riparia), which are primarily used to provide root resistance to the insect pest Phylloxera. In addition to Phylloxera resistance, ideally grapevine rootstocks should be resistant to other soil borne pathogens and adapted to abiotic stress conditions. New rootstocks have the potential to adapt agriculture to climate change without changing the characteristics of the harvested product. However, high grafting success rates are an essential prerequisite.