terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Abstract

Uruguay is known for the production of Tannat wines, which is a neutral variety from an aroma point of view, but capable of providing aromatic precursors that are of interest in the production of wines for ageing. The main aromatic precursors present are glycosidic compounds and carotenoids. The contribution of carotenoid degradation by-products such as norisoprenoids to wine aroma is fundamental, as they are associated with pleasant aroma descriptors and very low olfactory perception thresholds. Several factors have been shown to influence carotenoid concentrations in grapes, such as cultivar, climatic conditions, viticultural region, plant water status, exposure to sunlight and ripening stage. Norisoprenoids can be formed by chemical or enzymatic degradation of carotenoids. In this work, we studied two contrasting clones from the same plot as an experimental strategy to minimize the environmental factor and focus on the genes of interest. We evaluated glycosidic precursors (SPE-GC-MS), carotenoids (HPLC-DAD) and gene expression (RNA-Seq) in the selected clones during four stages of grape ripening. Significant differences in carotenoid and norisoprenoid content were found throughout the ripening period. Comparisons between clones showed significant differences in carotenoid content but not in norisoprenoid content during this harvest. Many genes associated with carotenoid and norisoprenoid biosynthesis showed differential expression throughout the ripening period in each clone. However, no genes were differentially expressed between clones. We conclude that the differences between clones do not manifest themselves every year. This shows that climate plays a fundamental role in aroma biosynthesis in Tannat clones.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Cecilia C. Da Silva1*, Nicolas Nieto2, Andres Coniberti3, Eduardo Boido2, Francisco Carrau2, Eduardo Dellacassa4, Laura Fariña2

1 PDU Espacio de Biología Vegetal del Noreste, sede Tacuarembó, CENUR Noreste, Universidad de la República, Tacuarembó, Uruguay
2 Área Enología y Biotecnología de Fermentaciones, CYTAL, Facultad de Química, Universidad de la República, Montevideo, Uruguay
3 Estación Experimental “Wilson Ferreira Aldunate”, Instituto Nacional de Investigación Agropecuaria, Canelones, Uruguay
4 Laboratorio de Biotecnología de Aromas, DQO, Facultad de Química, Universidad de la República, Montevideo, Uruguay

Contact the author*

Keywords

Carotenoid, Norisoprenoid, Tannat, GC-MS, RNA-Seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Differentiating and grouping of oltrepo’ pavese environments according to grape maturation

The maturation patterns process has been very studied. In particular the modelization of the sugars and titratable acidity during the ripening period was an important approach, in particular for the prediction of harvest date (Barillere et al., 1988; Jourion et al.,1987; Maujean et al., 1983; Scienza, 1989). In Oltrepò Pavese, the widest viticultural district of Lombardy – Northern Italy – (about 15000 hectares), grape maturation trends shows high variability, due to the large variation in environmental characteristics of vineyards (altitude, exposure, soil type, mesoclimate) and to “cultivar x environment” interaction.

Read More

Comparison of the effects of hormone- and natural-based elicitors on key metabolic pathways in cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.

Read More

Enhancing competitiveness through independent table grape breeding programs

Freeing seedless table grape producers from paying royalties on patents held by international breeding companies is a primary objective of the breeding programs promoted by the IVC

Read More

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.

Read More

Soil carbon changes and greenhouse gas emissions in vineyards – Is the 4 per 1000 goal realistic?

In this video recording of the IVES science meeting 2023, Hans Reiner Schultz (Hochschule Geisenheim University, Germany) speaks about soil carbon changes and greenhouse gas emissions in vineyards – is the 4 per 1000 goal realistic?. This presentation is based on an original article accessible for free on OENO One.

Read More