terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Characterizing the molecular basis of the differences in aromatic precursors found in commercial clones of Vitis vinifera cv. Tannat

Abstract

Uruguay is known for the production of Tannat wines, which is a neutral variety from an aroma point of view, but capable of providing aromatic precursors that are of interest in the production of wines for ageing. The main aromatic precursors present are glycosidic compounds and carotenoids. The contribution of carotenoid degradation by-products such as norisoprenoids to wine aroma is fundamental, as they are associated with pleasant aroma descriptors and very low olfactory perception thresholds. Several factors have been shown to influence carotenoid concentrations in grapes, such as cultivar, climatic conditions, viticultural region, plant water status, exposure to sunlight and ripening stage. Norisoprenoids can be formed by chemical or enzymatic degradation of carotenoids. In this work, we studied two contrasting clones from the same plot as an experimental strategy to minimize the environmental factor and focus on the genes of interest. We evaluated glycosidic precursors (SPE-GC-MS), carotenoids (HPLC-DAD) and gene expression (RNA-Seq) in the selected clones during four stages of grape ripening. Significant differences in carotenoid and norisoprenoid content were found throughout the ripening period. Comparisons between clones showed significant differences in carotenoid content but not in norisoprenoid content during this harvest. Many genes associated with carotenoid and norisoprenoid biosynthesis showed differential expression throughout the ripening period in each clone. However, no genes were differentially expressed between clones. We conclude that the differences between clones do not manifest themselves every year. This shows that climate plays a fundamental role in aroma biosynthesis in Tannat clones.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Cecilia C. Da Silva1*, Nicolas Nieto2, Andres Coniberti3, Eduardo Boido2, Francisco Carrau2, Eduardo Dellacassa4, Laura Fariña2

1 PDU Espacio de Biología Vegetal del Noreste, sede Tacuarembó, CENUR Noreste, Universidad de la República, Tacuarembó, Uruguay
2 Área Enología y Biotecnología de Fermentaciones, CYTAL, Facultad de Química, Universidad de la República, Montevideo, Uruguay
3 Estación Experimental “Wilson Ferreira Aldunate”, Instituto Nacional de Investigación Agropecuaria, Canelones, Uruguay
4 Laboratorio de Biotecnología de Aromas, DQO, Facultad de Química, Universidad de la República, Montevideo, Uruguay

Contact the author*

Keywords

Carotenoid, Norisoprenoid, Tannat, GC-MS, RNA-Seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Single plant oenotyping: a novel approach to better understand the impact of drought on red wine quality in Vitis x Muscadinia genotypes

Adopting disease-tolerant varieties is an efficient solution to limit environmental impacts linked to pesticide use in viticulture. In most breeding programs, these varieties are selected depending on their abilities to tolerate diseases, but little is known about their behaviour in response to abiotic constraints.

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease.

New antibacterial peptides produced by Saccharomyces cerevisiae responsible for the inhibition of malolactic fermentation

In winemaking, several antimicrobial peptides (AMPs) produced by different strains of Saccharomyces cerevisiae were found to be responsible for the inhibition of malolactic fermentation (MLF) carried out by some strains of Oenococcus oeni. However, only two AMPs produced by one of the yeast strains studied were totally identified and their mechanism of action was described. In an attempt to identify new AMPs, a 5-10 kDa peptidic fraction produced by an oenological strain of S. cerevisiae and previously shown to strongly inhibit MLF carried out by a strain of O. oeni was further purified.

Acumulación de materia seca, orientada a valorar la fijación de carbono, en función del aporte de riego y la pluviometría, en Cabernet-Sauvignon a lo largo de 15 años

The vineyard is capable of fixing carbon in its permanent structure from atmospheric carbon dioxide, through the process of gas exchange and the performance of photosynthesis. The photosynthetic capacity of the vineyard depends on the water resources that the plant may have at its disposal, so the amount of dry matter, derived from the processed photosynthates, that it can store will depend on the water regime of the crop, both in the annually renewable organs as in permanent parts.

Analisi delle modalita’ d’interazione tra conoscenza dl un territorio e gestione dei suoi aspetti enoturistici relativamente ad una regione (Toscana)

Il Turismo negli anni 2000 si appresta ad essere la più vasta ed estesa impresa presente nel mondo. Se a cio si aggiunge il fatto che il tempo libero risulta essere, per la maggior parte delle persone, la principale aspirazione da considerare ben oltre il proprio benessere eco­nomico, ci si rende conto del perché di una crescente domanda di ecoturismo. Un viaggiare sempre più accorto aile valenze ambientali e tipiche di un territorio.