Représentation holistique d’une dynamique pluridisciplinaire suite à la cartographie des sols en Beaujolais

In the production of sparkling wines, during the second fermentation, mannoproteins are released by yeast autolysis, which affect the quality of the wines. The effect of mannoproteins has been extensively studied, and may affect aroma and foam quality. However, there are no studies on the effect of other polysaccharides such as those from grapes. Considering the large production of waste from the wine industry, it was proposed to obtain polysaccharide-rich extracts from some of these by-products[1].

Eleven wine-growing sites are now on the UNESCO World Heritage List as Cultural Landscapes. If the viticultural character of these sites constitutes the main argument for the demonstration of their heritage value, the terroir and its biophysical and environmental characteristics tend however to appear in a minor mode compared to the aesthetic and cultural dimensions. In other words, the “specific characteristics of the soil, topography, climate, landscape and biodiversity” (OIV definition) are most often used as descriptive elements in the presentation of the sites, but it is more the aesthetic, historical,

In vineyards grown in warm areas, it is usual that the stage of maturity of the grapes is fast and easily reach a high concentration of sugar and low acidity, but not a adequate phenolic maturation. The geometry of the trellis system and the shoot density can modify the microclimate of the cluster and, therefore, the maturation process.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.