Terroir 2016 banner
IVES 9 IVES Conference Series 9 Impact of defoliation on leaf and berry compounds of Vitis vinifera L. Cv. Riesling investigated using non-destructive methods)

Impact of defoliation on leaf and berry compounds of Vitis vinifera L. Cv. Riesling investigated using non-destructive methods)

Abstract

Climate change has a strong impact on the earlier onset of important phenological stages and plant development in viticulture. Hence, the adaptation of plant management is important to reply to climate related changes on a seasonal or long-term scale. In particular, a change in precipitation and higher temperatures entails the risk negatively impacting on fruit quality. An experiment was conducted where different canopy management strategies were applied to Riesling grapevines (Vitis vinifera L. cv. Riesling) planted in Winkel (Rheingau, Germany). Leaf removal at different canopy positions using various methods (e.g. manual vs. mechanical defoliation practices) led to a reduced photosynthetic active leaf area. Through modifications of the leaf area to fruit weight ratio, the berry ripening can be altered. Leaf removal of the bunch zone impacts fruit parameter and most importantly fruit health. Four different defoliation practices within a VSP trellis system were compared to a non-defoliated control during three growing seasons in an organic treated site: mechanical defoliation above the canopy (MDC); manually defoliation prior to flowering (DpF); defoliation of the bunch zone past flowering: Bunch zone defoliation (BZD) either suction fan plucking (EB490® Binger Seilzug, Germany) and mechanical defoliation or pulsation jetting of compressed air (DmS) (Siegwald®, Germany). Non-destructive measurements using a polyphenolmeter (Multiplex®3, Force-A, Orsay, France) were performed on leaves and berries to estimate the nutrition and ripening stage.

The chlorophyll index showed the lowest values for BZD and highest for control leaves. Additionally, on-the-go measurements were established to determine leaf components achieving vineyard maps in response to nitrogen or chlorophyll index. Furthermore, the data can be used for zoning the vineyard and harvest based on such mapping. When the severity of Botrytis cinerea was compared to control all treatments showed lower disease pressure (BZD -5.3 %, DpF -3.0 % and DmS -2.3 % respectively). Yield differed between -16 % (MDC), -8% DpF, -1 % (DmS) and +1 % (BZD) compared to the control having the highest (1.4 g) and BZD the lowest (1.1 g) single berry weight with a lower bunch compactness in 2014. Defoliation treatments influence the number of cluster per vine, where the lowest were found for DpF plants, accompanied with the lowest yield per single vine. These results help understanding the canopy characteristics and offer an opportunity to adapt the vineyard management strategies to seasonal changes.

DOI:

Publication date: June 23, 2020

Issue: Terroir 2016

Type: Article

Authors

Susanne TITTMANN, Vanessa STOEBER, Manfred STOLL

Geisenheim University, Department of general and organic viticulture, Von – Lade – Str. 1 D-65366 Geisenheim

Contact the author

Keywords

defoliation, non-invasive determination of leaf components, Multiplex, Plasmopara viticola, Vitis vinifera

Tags

IVES Conference Series | Terroir 2016

Citation

Related articles…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

1H-NMR-based Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

The aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through untargeted and targeted 1H-NMR metabolomics. One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz. The spectra were recorded by applying the NOESYGPPS1D pulse sequency, to achieve water and ethanol signals suppression. No modification of the pH was performed to avoid any chemical alteration of the matrix. The generation of input variables for untargeted analysis was done via bucketing the spectra. The resulting dataset was preprocessed prior to perform unsupervised PCA, by means of MetaboAnalyst web-based tool suite. The identification of compounds for the targeted analysis was performed by comparison to pure compounds spectra by means of SMA plug-in of MNova 14.2.3 software. The dataset containing the concentrations (%) of identified compounds was subjected to one-way analysis of variance (ANOVA) to highlight significant differences among the wines. The untargeted analysis, carried out through the PCA, revealed a clear differentiation among the wines. The fragments of the spectra contributing mostly to the separation were attributed to flavonoids, aroma compounds and amino acids. The targeted analysis leaded to the identification of 68 compounds, whose concentrations were significant different among the wines. The results were related to soils physical-chemical analysis and showed that: 1) high concentrations of flavan-3-ols and flavonols are correlated with high clay content in soils; 2) high concentrations of anthocyanins, amino acids, and aroma compounds are correlated with neutral and moderately alkaline soil pH; 3) low concentrations of flavonoids and aroma compounds are correlated with high soil organic matter content and acidic pH. The 1H-NMR metabolomic analysis proved to be an excellent tool to discriminate between wines originating from grapes grown on different soil types and revealed that soils in the Mediterranean area exert a strong impact on the chemical composition of the wines.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.