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IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2010 9 Impact of microbiological and enological techniques on wine macromolecules 9 Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines

Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines


In this study for the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin,trans-piceatannol, (+)-trans-s-viniferin, pallidol and hopeaphenol) isolated and purified from Vitis vinifera, were simultaneously separated and analysed within 5 mn by ultra high pressure liquid chromatography coupled with photodiode array detection. The separation has been optimized using quality-by-design software allowing to get the quality by design and fast optimization of UHPLC and to study the influence of the ultra high pressure on the prediction. Four different reversed phase materials of subparticle size were tested and compared on the basis of retention behaviour, separation efficiency and matrix effect of the directly injected wine. Characteristics of the method showed highrepeatability of retention times (RSD 0.03-0.07 %) and peak areas (RSD=3-6 %) on the determined linear range (between 0.005-50 mg L-1) for most components. Five of these stilbenes were identified and quantified in Burgundy red wines at different concentrations without any sample preparation steps, thus enabling a straightforward stilbene-based metabologeographical approach of wine analysis.

Publication date: July 3, 2024

Issue: Macrowine 2010

Type: Article



1 EA 581 EMMA, Institut Universitaire de la vigne et du vin-Jules Guyot, Université de Bourgogne, Rue Claude Ladrey, 21078 Dijon, F.

2Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764 Neuherberg, D.

3Université de Bordeaux 2, Groupe d’étude des substances végétales à activité biologique, EA 3615, IFR 103, Institut des Sciences de la Vigne et du Vin (ISVV), 210 chemin de Leysotte, cs 50008 Villenave d’Ornon, F.

4 Université de Reims, Unité de recherche Vigne et Vins de Champagne, EA 2069, Campus Moulin de la House Bat 8-BP1039, 51687 Reims, Cedex 2, F.

Contact the author*


stilbenes, wine, UHPLC


IVES Conference Series | Macrowine | Macrowine 2010


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