Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines

Abstract

In this study for the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin,trans-piceatannol, (+)-trans-s-viniferin, pallidol and hopeaphenol) isolated and purified from Vitis vinifera, were simultaneously separated and analysed within 5 mn by ultra high pressure liquid chromatography coupled with photodiode array detection. The separation has been optimized using quality-by-design software allowing to get the quality by design and fast optimization of UHPLC and to study the influence of the ultra high pressure on the prediction. Four different reversed phase materials of subparticle size were tested and compared on the basis of retention behaviour, separation efficiency and matrix effect of the directly injected wine. Characteristics of the method showed highrepeatability of retention times (RSD 0.03-0.07 %) and peak areas (RSD=3-6 %) on the determined linear range (between 0.005-50 mg L^-1) for most components. Five of these stilbenes were identified and quantified in Burgundy red wines at different concentrations without any sample preparation steps, thus enabling a straightforward stilbene-based metabologeographical approach of wine analysis.