Evolution of biogenic amines content in wine during sample conservation – method optimisation for analysis of biogenicamines

Abstract

The present paper reports the development of an optimized method for simultaneous analysis of 8 biogenic amines (Histamine, Methylamine, Ethylamine, Tyramine, Putrescine, Cadaverine, Phenethylamine, and Isoamylamine). It is based on a method developed by Gomez-Alonso et al. in 2007. The proposed analytical method has the following advantages: easy derivatization of wines, quantification of biogenic amines, and complete degradation of excess derivatization reagent during sample preparation to preserve column. It consists of reversed phase separation by HPLC and UV- vis detection of the aminoenones formed by the reaction of amino compounds with the derivatizationreagent diethyl ethoxymethylenemalonate (DEEMM). The technique was confirmed with an alternative oenological analysis method for the validation, quality control and uncertainty assessment (OIV Oeno 10/2005).