Plastid genomics of Vitis vinifera L. for understanding the molecular basis of grapevine (Vitis vinifera L.) domestication
Abstract
The precise molecular mechanisms underlying the domestication of grapevine (Vitis vinifera L.) Are still not fully understood. In the recent years, next-generation sequencing (NGS) of plastid genomes has emerged as a powerful and increasingly effective tool for plant phylogenetics and evolution. To uncover the biological profile of the grapevine domestication process comprehensively, an investigation should encompass both the cultivated varieties (v. Vinifera subsp. Vinifera) and their wild ancestors (V. vinifera subsp. Sylvestris) across all potential sites of their distribution and domestication. The main goals of the presented research were as follows: 1) assessment of the genetic diversity of wild and cultivated grapevines from Europe and mediterenean basein (wild ones of France, Germany, Italy, Spain, Portugal, Hungary, Morocco) and georgia, caucasus (both cultivated and wild), american and asian spieces (i.e. V. Californica, v. Thunbergii) through complete chloroplast dna illumina sequencing (more than 30 plastoms in total); 2) in silico assembly and annotation of the sequenced plastid genomes; 3) comparative genomics and phylogenetics of a wide range of Vitis vinifera l. Plastoms. The results revealed the dominance of the gta plastid haplotype in europe and the mediterranean basin, except of portuguese wild grapevine; the diversity of plastid haplotypes in the south caucasus, especially in georgia, is once again demonstrated, highlighting the potential role of this geographic region in the grapevine domestication process; in each analyzed plastomes, a total of 128 genes (protein- coding, tRNA, and rRNA genes) were annotated. Furthermore, comparative genomics approaches were employed to evaluate the phylogenetic relationships among different Vitis vinifera L. Chloroplast genomes.
Issue: OIV 2024
Type: Article
Authors
¹ Agricultural University of Georgia, Institute of Molecular Genetics, David Aghmashenebeli Alley 240, Tbilisi, Georgia
² AGAP, Université de Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France