La place du terroir dans le processus de patrimonialisation : l’exemple des paysages culturels viticoles du patrimoine mondial de l’Unesco

Temperature is a key environmental factor affecting grape primary and secondary metabolites. Even if several mesoscale studies have already been conducted on temperature especially within a Protected Designation of Origin area, few data are available at an intra-block scale. The present study aimed at i) assessing the variability in bunch zone air temperature within a single vineyard block and the temporal stability of temperature spatial patterns, ii) understanding temperature drivers and iii) identifying the impact of temperature on grape berry attributes.

Deciphering grapevine dormancy is crucial in the current context of climatic challenges: advancing budburst phenology and increased late frost probabilities, observed in the last decades and expected to further increase, require deeper understanding. Beyond higher mean temperatures, abiotic stresses such as water deficit have also been emphasized as actors. In this framework, we aimed at exploring new methodologies for tracking dormancy cycle and testing the interplay on its regulation of temperature dynamics and drought.
In a first experiment, twenty-one Vitis vinifera varieties were monitored during ecodormancy and budburst over three years.

Obtaining high-quality RNA from grape tissues, including berry pulp, berry skins, stems, rachis, or roots, is challenging due to their composition, which includes polysaccharides, phenolic compounds, sugars, and organic acids that can negatively affect RNA extraction. For instance, polyphenols and other secondary metabolites can bind to RNA, making it difficult to extract a pure sample. Additionally, RNA can co-precipitate with polysaccharides, leading to lower extraction yield. Also, sugars and organic acids can interfere with the pH and ionic properties of the extraction buffer. To address these challenges, we optimized a protocol for RNA isolation from grape tissues.

The components and the methodology for characterization of the terroir in Spain have been described by Gómez-Miguel et al.

Volatile sulphur compounds are a group of impact odorants with low odour thresholds that can contribute both positively and negatively to wine aroma. The varietal thiols, 3MH and 3MHA, are known to contribute positive tropical aromas to white wines and are most abundant in Sauvignon Blanc wines. The group of compounds contributing negative aromas are known as reductive sulphur compounds (RSCs) as they add a reductive aroma of asparagus, cooked vegetables and rotten egg to wines. All these compounds play a part in and are a result of the sulphur pathway in the yeast cell during fermentation and therefore attempting to increase the concentration of the varietal thiols may directly influence the concentration of the RSCs. The varietal thiols and the low molecular weight RSCs are highly volatile and therefore their sensory perception can change rapidly over time.