Terroir 2014 banner
IVES 9 IVES Conference Series 9 Volatile organic compounds: a role in elicitor-induced resistance of grapevine against pathogens?

Volatile organic compounds: a role in elicitor-induced resistance of grapevine against pathogens?

Abstract

As Vitis vinifera varieties are susceptible to fungal diseases, numerous chemical treatments are generally required to ensure the quantity and quality of the harvest. However, in the context of sustainable viticulture, there are increasing societal request, political incitation, and winegrowers’ awareness to reduce the use of pesticides. Among possible solutions the use of elicitors that could be included in integrated pest management or biocontrol strategies might be very promising. These bioactive compounds are able to trigger plant defences, leading to induced resistance (IR) against pathogens. Despite IR can be elicited very successfully in controlled environments; it is in most cases not effective enough in practically controlling disease in the vineyard.

To obtain a comprehensive understanding of IR and to identify molecular markers enabling the identification of factors (physiological, environmental…) that can impact IR efficacy in the vineyard we performed a transcriptomic analysis under controlled conditions. The results indicated that among highly up-regulated genes associated to IR, one was annotated as terpene synthase, suggesting that terpenes could be emitted following elicitor treatment. This prompted us to investigate whether IR elicitors actually induce the production of volatile organic compounds (VOCs). Applying online analysis (PTR-QMS) of VOC emissions in dynamic cuvettes and passive sampling in gas tight bags with solid phase micro extraction (SPME / GC-MS), we followed the emission of VOCs of vines in response to elicitor-IR against downy mildew under controlled greenhouse conditions.

The results obtained point out some of them as potential markers of elicitor-IR (as trans a-farnesene) whereas MeSA is rather a marker of downy mildew infection.

DOI:

Publication date: August 18, 2020

Issue: Terroir 2014

Type: Article

Authors

Marielle Adrian (1), Malik Chalal (1), Barbro Winkler (2), Karine Gourrat (3), Jörg Schnitzler (2), Xavier Daire (4)

(1) Université de Bourgogne, UMR AgroSup/INRA/uB 1347 Agroécologie, Pôle IPM – ERL CNRS 6300, Dijon, France 
(2) Research Unit EUS, Helmholtz Zentrum München, Germany 
(3) ChemoSens, INRA Dijon, France 
(4) Inra, UMR AgroSup/INRA/uB 1347 Agroécologie, Pôle IPM – ERL CNRS 6300, Dijon, France 

Contact the author

Keywords

grapevine, elicitor, induced resistance, downy mildew, VOCs

Tags

IVES Conference Series | Terroir 2014

Citation

Related articles…

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic composition of the produced wines. A series of wood chips, originating from American, French, Slavonia oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples were analyzed one month after the end of malolactic fermentation, examining various chemical parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and ellagitannin content.

The use of rootstock as a lever in the face of climate change and dieback of vineyard

As viticulture faces challenges such as climate change or vineyard dieback, the choice of the variety and rootstock becomes more and more crucial. To study rootstock levers in the Bordeaux region, a parcel of Cabernet Sauvignon (CS) was planted with four rootstocks in 2014. Twenty repetitions of each of the following four rootstocks were set up: 101-14 MGt, Nemadex AB, 420A MGt and Gravesac. The number of bunches, yields and pruning weights of the vine shoots were measured individually on 240 vines from 2017 to 2021. Since 2020, nitrogen status assessed by assimilable nitrogen level, hydric status assessed by δ13C and berry maturity were measured on 80 samples taken from 20 repetitions of the four rootstocks. A lower yield was measured for CS grafted onto Nemadex AB due to the lower number of bunches and the lower weight of berries. The differences between the other three rootstocks are small, but CS grafted onto 420A MGt was the most productive. The CS grafted onto Nemadex AB had the lowest pruning weight while 101-14 MGt had the highest. In 2020, δ13C showed a more moderate water stress with 101-14 MGt and 420A MGt than with Nemadex AB. Surprisingly, the Gravesac was under more stress than the 101-14 MGt. The nitrogen status in the berries was better for Nemadex AB but this was perhaps due to the significantly lower weight of the berries.Rootstock 101-14 MGt attained the highest accumulation of sugars in the berries while 420A MGt allows to preserve higher acidity. The parcel is still young which may explain some of the results. These measures must therefore be continued over the next several years to fully assess the effects of these rootstocks on the development of the vines and the quality of the production under new climatic conditions.

Volatile compounds as indicators of terroir differentiation in Moldovan Feteasca Neagra wines

This study examined volatile compounds in Feteasca Neagra wines from seven vineyards across three PGI regions in Moldova using GC-IMS.

Untargeted metabolomics to identify potential chemical markers responsible for the permissiveness of red wines against Brettanomyces bruxellensis

Red wines constitute the majority of the wines produced in Bordeaux. All along the winemaking process, many microorganisms may develop in wine. A lot of them are useful but a common defect found in wine is linked to the development of Brettanomyces bruxellensis, a yeast that produces volatile phenols. These molecules are responsible for an unwanted sensorial defect described as similar to “horse sweat”, “burnt plastic” or “leather”. It has been shown that while some wines are very permissive and easily contaminated, others are pretty resistant to Brettanomyces development. However, common parameters such as pH, alcohol or sugars composition cannot fully explain the differences observed in wine permissiveness.

Global warming effects on grape growing climate zones within the Rioja Appllation (DOCa Rioja) in north Spain

Aims: The aims of this work were (1) to assess the changes in some of the main bioclimatic indices used for climate viticultural zoning within the Rioja Appellation area in the north of Spain between 1950-2014 (60 years), and (2) to carry out a comprehensive sociological evaluation among grapegrowers and winemakers of this region, to better understand the impact of climate change on their activity, their degree of concern about it and the potential adaptation measures they would be willing to adopt to cope with it in future years.