Geostatistical analysis of the vineyards in the canton of Geneva in relation to soil and climate

The French vine selection partners network is currently made up of 40 regional partners, grouped around IFV (French Institute for Vine and Wine) and INRAE (national research institute for agriculture and environment), whose missions are preservation, selection, and innovation of our varietal diversity. The originality of this device is based on a 3-level organisation: – varietal diversity preservation, with the world reference: the INRAE’s vine genetics resources centre of Vassal-Montpellier (Marseillan, France), the world’s largest ampelographic collection, which includes nearly 6 000 accessions of cultivated Vitis vinifera from 54 countries, as well as rootstocks, interspecific hybrids, wild vines (lambrusques) and wild American and Asian species.

Unmanned Aerial Vehicles (UAVs) are increasingly used to monitor canopy structure and vineyard performance. Compared with traditional remote sensing platforms (e.g. aircraft and satellite), UAVs offer a higher operational flexibility and can acquire ultra-high resolution images in formats such as true color red, green and blue (RGB) and multispectral. Using photogrammetry, 3D vineyard models and normalized difference vegetation index (NDVI) maps can be created from UAV images and used to study the structure and health of grapevine canopies. However, there is a lack of comparison between UAV-based images and ground-based measurements, such as leaf area index (LAI) and canopy porosity.

Botrytis bunch rot occurrence is the most important limitation for the wine industry in humid environments. The effect of grapevine vegetative growth on bunch rot expression results from direct effects (cluster architecture, nitrogen status among others) and indirect ones (via microclimate). Previous studies of our group showed strong differences in bunch rot incidence between floor management treatments: cover crop (CC) vs weed-free strips under the trellis with herbicide (H). We observed that in some circumstances this reduction in bunch rot incidence occurred without major vine growth differences among treatments. The aim of the present study was to test the general hypothesis that other factors unrelated to grapevine vegetative expression could be more relevant to grapevine susceptibility to bunch rot.

Le sujet proposé dans le thème “l’environnement juridique” est plus économique que juridique, et constitue une sorte de complément au sujet qui l’a précédé : analyse des marchés, stratégies commerciales et terroirs”.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.