Multi-omic dissection of interspecific grapevine rootstock-scion-environment interactions in the United States
Abstract
Gene regulation in grapevine emerges from interconnected above- and below-ground responses to the environment. Bulk molecular profiling has largely focused on scion cultivars, primarily Vitis vinifera, and has provided important insights for genotype-by-environment (G×E) interactions. As viticulture adapts to increasing climatic pressures, it is essential to evaluate these approaches in newly adoptedinterspecific hybrids and across the distinct climates where they are cultivated. Here, interspecific hybrid ‘Marquette’ scions werereplicated across three experimental vineyard sites in the United States and grafted to multiple rootstocks (1103P, 3309C, SO4, 5C), alongside homografted ‘Marquette’ and own-rooted ‘Marquette’ individuals. Leaf, berry, and root tissues were collected for multi-omic and phenotypic profiling, including transcriptomics, metabolomics, anatomy, epigenetics, and ionomics, to quantify the relative contributions of site and environment to scion genotype × rootstock genotype × environment interactions. Together, these datasets span distinct climatic regimes that strongly influence phenotypic plasticity and provide a mechanistic framework linking genotype, gene regulation, and observed phenotype.
Acknowledgements
This work was supported by a Plant Genome Research grant from the National Science Foundation (Award #2431712).
Issue: GBG 2026
Type: Poster
Authors
1 Cornell University, Horticulture Section, New York, USA
2 Saint Louis University, Department of Biology, Missouri, USA
3 Donald Danforth Plant Science Center, Missouri, USA
4 Pennsylvania State University, Department of Food Science, Pennsylvania, USA
5 South Dakota State University, Agronomy, Horticulture and Plant Science Department, South Dakota, USA
6 Missouri State University, Department of Biology, Missouri, USA