Terroir 2020 banner
IVES 9 IVES Conference Series 9 Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Abstract

Aims: To investigate the intraregional variation of varietal thiol precursors and free thiols in Sauvignon blanc grape juices and experimental wines arising from the Adelaide Hills Geographical Indication (GI) in South Australia.

Methods and ResultsVitis vinifera L. cv Sauvignon blanc grape parcels (n = 21, approx. 8 kg each, encompassing 5 clones) were hand harvested from different blocks within seven commercial vineyards in the Adelaide Hills GI during the 2018 vintage. Parcels were divided into subsets for winemaking and freezing experiments. Amino acid (AA) and thiol precursor concentrations in juice were determined using high performance liquid chromatography (HPLC) with fluorescence detection and stable isotope dilution assay (SIDA) using HPLC with tandem mass spectrometry (MS/MS), respectively, and free thiols in wine were quantified by SIDA with HPLC-MS/MS, after derivatisation with 4,4’-dithiodipyridine. Intraregional variations in grape ripeness were evident according to total soluble solids content, pH, and titratable acidity, even within single locations or for the same clones. Significant differences in the glutathionylated precursor to 3-sulfanylhexan-1-ol (3-SH) were found among several locations whereas for the cysteinylated variant of 3-SH, one location was distinct from the rest. Variation in precursor concentrations was also noted from different blocks within a single vineyard location but was not dependent on grape ripeness. Fermentations progressed without any obvious relationship to location, and wines that were high in 3-SH were also usually high in 3-sulfanylhexyl acetate (3-SHA). One location had significantly higher levels of thiols in wine despite the juice not being the highest for grape-derived precursors, and also gave a substantial concentration of 4-methyl-4-sulfanylpentan-2-one in comparison to other locations within the GI. The AA profile of juices was found to vary according to location, and certain AAs were strongly correlated to thiol precursor concentrations, but relationships of AAs with free thiols in wine were generally weak. Additionally, enhancements in the concentrations of precursors in juice (up to 19-fold) and free thiols in wine (up to 10-fold) were revealed from freezing whole grape bunches in contrast to using fresh juice.

Conclusions: 

Intraregional variation was noted for thiols in wine, and precursors and amino acids in juice, for 21 Sauvignon blanc samples collected from within the Adelaide Hills region. The effects of terroir were implicated in explaining the differences in grape composition, and the potential interactions among grape amino acids and thiol precursors in berries and thiols in wine were revealed.

Significance and Impact of the Study: Sauvignon blanc is a significant variety produced in the Adelaide Hills GI but no information was available on the effects of location within the GI on grape and wine composition with respect to varietal thiols. This was the first study of intraregional variations of thiol precursors, amino acids, and free thiols in Sauvignon blanc juices and wines that were produced in a consistent manner. A remarkable enhancing effect of freezing was noted for thiol precursors in juice, and importantly, free thiols in wine.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Liang Chen1,a, Dimitra L. Capone1,2, Emily L. Nicholson3, David W. Jeffery1,2*

1 School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia
2 Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, PMB 1 Glen Osmond, SA 5064, Australia
3 CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA 5064, Australia

aPresent address: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux, 210 chemin de Leysotte CS 50008, 33882 Villenave d’Ornon Cedex, France

Contact the author

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.

1H-NMR-based Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

The aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through untargeted and targeted 1H-NMR metabolomics. One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz. The spectra were recorded by applying the NOESYGPPS1D pulse sequency, to achieve water and ethanol signals suppression. No modification of the pH was performed to avoid any chemical alteration of the matrix. The generation of input variables for untargeted analysis was done via bucketing the spectra. The resulting dataset was preprocessed prior to perform unsupervised PCA, by means of MetaboAnalyst web-based tool suite. The identification of compounds for the targeted analysis was performed by comparison to pure compounds spectra by means of SMA plug-in of MNova 14.2.3 software. The dataset containing the concentrations (%) of identified compounds was subjected to one-way analysis of variance (ANOVA) to highlight significant differences among the wines. The untargeted analysis, carried out through the PCA, revealed a clear differentiation among the wines. The fragments of the spectra contributing mostly to the separation were attributed to flavonoids, aroma compounds and amino acids. The targeted analysis leaded to the identification of 68 compounds, whose concentrations were significant different among the wines. The results were related to soils physical-chemical analysis and showed that: 1) high concentrations of flavan-3-ols and flavonols are correlated with high clay content in soils; 2) high concentrations of anthocyanins, amino acids, and aroma compounds are correlated with neutral and moderately alkaline soil pH; 3) low concentrations of flavonoids and aroma compounds are correlated with high soil organic matter content and acidic pH. The 1H-NMR metabolomic analysis proved to be an excellent tool to discriminate between wines originating from grapes grown on different soil types and revealed that soils in the Mediterranean area exert a strong impact on the chemical composition of the wines.