Terroir 2020 banner
IVES 9 IVES Conference Series 9 Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Abstract

Aims: To investigate the intraregional variation of varietal thiol precursors and free thiols in Sauvignon blanc grape juices and experimental wines arising from the Adelaide Hills Geographical Indication (GI) in South Australia.

Methods and ResultsVitis vinifera L. cv Sauvignon blanc grape parcels (n = 21, approx. 8 kg each, encompassing 5 clones) were hand harvested from different blocks within seven commercial vineyards in the Adelaide Hills GI during the 2018 vintage. Parcels were divided into subsets for winemaking and freezing experiments. Amino acid (AA) and thiol precursor concentrations in juice were determined using high performance liquid chromatography (HPLC) with fluorescence detection and stable isotope dilution assay (SIDA) using HPLC with tandem mass spectrometry (MS/MS), respectively, and free thiols in wine were quantified by SIDA with HPLC-MS/MS, after derivatisation with 4,4’-dithiodipyridine. Intraregional variations in grape ripeness were evident according to total soluble solids content, pH, and titratable acidity, even within single locations or for the same clones. Significant differences in the glutathionylated precursor to 3-sulfanylhexan-1-ol (3-SH) were found among several locations whereas for the cysteinylated variant of 3-SH, one location was distinct from the rest. Variation in precursor concentrations was also noted from different blocks within a single vineyard location but was not dependent on grape ripeness. Fermentations progressed without any obvious relationship to location, and wines that were high in 3-SH were also usually high in 3-sulfanylhexyl acetate (3-SHA). One location had significantly higher levels of thiols in wine despite the juice not being the highest for grape-derived precursors, and also gave a substantial concentration of 4-methyl-4-sulfanylpentan-2-one in comparison to other locations within the GI. The AA profile of juices was found to vary according to location, and certain AAs were strongly correlated to thiol precursor concentrations, but relationships of AAs with free thiols in wine were generally weak. Additionally, enhancements in the concentrations of precursors in juice (up to 19-fold) and free thiols in wine (up to 10-fold) were revealed from freezing whole grape bunches in contrast to using fresh juice.

Conclusions: 

Intraregional variation was noted for thiols in wine, and precursors and amino acids in juice, for 21 Sauvignon blanc samples collected from within the Adelaide Hills region. The effects of terroir were implicated in explaining the differences in grape composition, and the potential interactions among grape amino acids and thiol precursors in berries and thiols in wine were revealed.

Significance and Impact of the Study: Sauvignon blanc is a significant variety produced in the Adelaide Hills GI but no information was available on the effects of location within the GI on grape and wine composition with respect to varietal thiols. This was the first study of intraregional variations of thiol precursors, amino acids, and free thiols in Sauvignon blanc juices and wines that were produced in a consistent manner. A remarkable enhancing effect of freezing was noted for thiol precursors in juice, and importantly, free thiols in wine.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Liang Chen1,a, Dimitra L. Capone1,2, Emily L. Nicholson3, David W. Jeffery1,2*

1 School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia
2 Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, PMB 1 Glen Osmond, SA 5064, Australia
3 CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA 5064, Australia

aPresent address: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux, 210 chemin de Leysotte CS 50008, 33882 Villenave d’Ornon Cedex, France

Contact the author

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Influence of agronomic practices in soil water content in mid-mountain vineyards

In the context of LIFE project MIDMACC (LIFE18 CCA/ES/001099), several pilots have been installed in vineyards in mid mountain areas of Catalonia (NE Spain) to test well stablished agronomic practices to increase the adaptation of Mediterranean mid mountain to climate change. Soil water content (SWC) at three different depths (15, 30 and 45cm) was measured in continuum from August 2020. One pilot (WC) included a well-established green cover (GC), a new GC (NC) and a conventional soil management (CM, tilling+herbicides). NC presented an intermediate state between WC and CM, responding similarly to CM in autumn but quickly reaching similar SWC to WC, then following the same evolution till next spring, with CM presenting lower values along autumn and winter. Then vegetation activation decreased SWC in all plots, (much slower in CM, lacking GC). Sensibility to spring rains is again intermediate for NC, which joins SWC evolution of CM by the end of spring till next autumn. It is expected that NC will resemble WC more and more as its GC develops. In the pilot combining vine training (VSP vs Gobelet) and hillside management (slope vs terrace), no clear pattern could be related with these conditions. However, both terraces seem to be more sensitive to spring rains. A third pilot included new vineyards (7 and 1 year old). In the new vineyard (N), higher canopy development, a spontaneous green cover and row straw resulted in a slower SWC dynamic, not so sensitive to rains but conserving more soil water in spring and most of summer, even with presumably a higher water extraction by vines. In the newest vineyard (VN) the deepest sensor is still sensitive to rain events all over the year and SWC is always highest at this depth, revealing small water capture by vines.

Adaptation to soil and climate through the choice of plant material

Choosing the rootstock, the scion variety and the training system best suited to the local soil and climate are the key elements for an economically sustainable production of wine. The choice of the rootstock/scion variety best adapted to the characteristics of the soil is essential but, by changing climatic conditions, ongoing climate change disrupts the fine-tuned local equilibrium. Higher temperatures induce shifts in developmental stages, with on the one hand increasing fears of spring frost damages and, on the other hand, ripening during the warmest periods in summer. Expected higher water demand and longer and more frequent drought events are also major concerns. The genetic control of the phenotypes, by genomic information but also by the epigenetic control of gene expression, offers a lot of opportunities for adapting the plant material to the future. For complex traits, genomic selection is also a promising method for predicting phenotypes. However, ecophysiological modelling is necessary to better anticipate the phenotypes in unexplored climatic conditions Genetic approaches applied on parameters of ecophysiological models rather than raw observed data are more than ever the basis for finding, or building, the ideal varieties of the future.

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

Towards a regional mapping of vine water status based on crowdsourcing observations

Monitoring vine water status is a major challenge for vineyard management because it influences both yield and harvest quality. It is also a challenge at the territorial scale for identifying periods of high water restriction or zones regularly impacted by water stress. This information is of major importance for defining collective strategies, anticipating harvest logistic or applying for irrigation authorisation. At this spatial scale, existing tools and methods for monitoring vine water status are few and often require strong assumptions (e.g. water balance model). This paper proposes to consider a collaborative collection of observations by winegrowers and wine industry stakeholders (crowdsourcing) as an interesting alternative. Indeed, it allows the collection of a large number of field observations while pooling the collection effort. However, the feasibility of such a project and its interest in monitoring vine water status at regional scale has never been tested.

The objective of this article is to explore the possibility of making a regional map of vine water status based on crowdsourcing observations. It is based on the study of the free mobile application ApeX-Vigne, which allows the collection of observations about vine shoot growth. This information is easy to collect and can be considered, under certain conditions, as a proxy for vine water status. This article presents the first results obtained from the nearly 18,000 observations collected by winegrowers and wine industry stakeholders during 2019, 2020 and 2021 seasons. It presents the vine shoot growth maps obtained at regional scale and their evolution over the three vintages studied. It also proposes an analysis of the factors that favoured the number of observations collected and those that favoured their quality. These results open up new perspectives for monitoring vine water status at a regional scale but above they provide references for other crowdsourcing projects in viticulture.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.