Terroir 2020 banner
IVES 9 IVES Conference Series 9 Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Abstract

Aims: To investigate the intraregional variation of varietal thiol precursors and free thiols in Sauvignon blanc grape juices and experimental wines arising from the Adelaide Hills Geographical Indication (GI) in South Australia.

Methods and ResultsVitis vinifera L. cv Sauvignon blanc grape parcels (n = 21, approx. 8 kg each, encompassing 5 clones) were hand harvested from different blocks within seven commercial vineyards in the Adelaide Hills GI during the 2018 vintage. Parcels were divided into subsets for winemaking and freezing experiments. Amino acid (AA) and thiol precursor concentrations in juice were determined using high performance liquid chromatography (HPLC) with fluorescence detection and stable isotope dilution assay (SIDA) using HPLC with tandem mass spectrometry (MS/MS), respectively, and free thiols in wine were quantified by SIDA with HPLC-MS/MS, after derivatisation with 4,4’-dithiodipyridine. Intraregional variations in grape ripeness were evident according to total soluble solids content, pH, and titratable acidity, even within single locations or for the same clones. Significant differences in the glutathionylated precursor to 3-sulfanylhexan-1-ol (3-SH) were found among several locations whereas for the cysteinylated variant of 3-SH, one location was distinct from the rest. Variation in precursor concentrations was also noted from different blocks within a single vineyard location but was not dependent on grape ripeness. Fermentations progressed without any obvious relationship to location, and wines that were high in 3-SH were also usually high in 3-sulfanylhexyl acetate (3-SHA). One location had significantly higher levels of thiols in wine despite the juice not being the highest for grape-derived precursors, and also gave a substantial concentration of 4-methyl-4-sulfanylpentan-2-one in comparison to other locations within the GI. The AA profile of juices was found to vary according to location, and certain AAs were strongly correlated to thiol precursor concentrations, but relationships of AAs with free thiols in wine were generally weak. Additionally, enhancements in the concentrations of precursors in juice (up to 19-fold) and free thiols in wine (up to 10-fold) were revealed from freezing whole grape bunches in contrast to using fresh juice.

Conclusions: 

Intraregional variation was noted for thiols in wine, and precursors and amino acids in juice, for 21 Sauvignon blanc samples collected from within the Adelaide Hills region. The effects of terroir were implicated in explaining the differences in grape composition, and the potential interactions among grape amino acids and thiol precursors in berries and thiols in wine were revealed.

Significance and Impact of the Study: Sauvignon blanc is a significant variety produced in the Adelaide Hills GI but no information was available on the effects of location within the GI on grape and wine composition with respect to varietal thiols. This was the first study of intraregional variations of thiol precursors, amino acids, and free thiols in Sauvignon blanc juices and wines that were produced in a consistent manner. A remarkable enhancing effect of freezing was noted for thiol precursors in juice, and importantly, free thiols in wine.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Liang Chen1,a, Dimitra L. Capone1,2, Emily L. Nicholson3, David W. Jeffery1,2*

1 School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia
2 Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, PMB 1 Glen Osmond, SA 5064, Australia
3 CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA 5064, Australia

aPresent address: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux, 210 chemin de Leysotte CS 50008, 33882 Villenave d’Ornon Cedex, France

Contact the author

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Mechanisms involved in the heating of the environment by the aerodynamic action of a wind machine to protect a vineyard against spring frost

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.

Current climate change in the Oplenac wine-growing district (Serbia)

Serbian autochthonous vine varieties Smederevka (for white wines) and Prokupac (for rosé and red wines) are the primary representatives of typical characteristics of wines and terroir of numerous wine-growing areas in Serbia. In the past, these varieties were the leading vine varieties, however, as the result of globalization of winemaking and the trend of consumption of wines from widely prevalent vine varieties, they were replaced by introduced international varieties. Smederevka and Prokupac vine varieties are characterized by later time of grape ripening, and relative sensitivity to low temperatures. Climate conditions can be a restrictive factor for production of high-quality grapes and wine and for the spatial spreading of these varieties in hilly continental wine-growing areas.
This paper focuses on the spatial analysis of changes of main climate parameters, in particular, analysis of viticultural bioclimatic indices that were determined for the purposes of viticulture zoning of wine-growing areas in the period 1961-2010, and those same parameters determined for the current, that is, referential climate period (1988-2017). Results of the research, that is, analysis of climate changes indicate that the majority of examined climate parameters in the Oplenac wine-growing district improved from the perspective of Smederevka and Prokupac vine varieties. These studies of climate conditions indicate that changes of analyzed climate parameters, that is, bioclimatic indices will be favorable for cultivation of varieties with later grape ripening times and those more sensitive to low temperatures, such as the autochthonous vine varieties Smederevka and Prokupac, therefore, it is recommended to producers to more actively plant vineyards with these varieties in the territory of the Oplenac wine-growing district.

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.

Leaf vine content in nutrients and trace elements in La Mancha (Spain) soils: influence of the rootstock

The use of rootstock of American origin has been the classic method of fighting against Phylloxera for more than 100 years. For this reason, it is interesting to establish if different rootstock modifies nutrient composition as well as trace elements content that could be important for determining the traceability of the vine products. A survey of four classic rootstocks (110-Richter, SO4, FERCAL and 1103-Paulsen) and four new ones (M1, M2, M3 and M4) provided by Agromillora Iberia. S.L.U., all of them grafted with the Tempranillo variety, has been carried out during 2019. The eight rootstocks were planted in pots of 500 cc, on three soils with very different characteristics from Castilla-La Mancha (Spain). In the month of July, the leaves were collected and dried in a forced air oven for seven days at 40ºC. Then, the samples were prepared for the analysis determination, carried out by X-Ray fluorescence spectrometry. The results obtained showed that in the case of content in mineral elements in leaf, separated by soil type, we can report the importance of few elements such as Si, Fe, Pb and, especially, Sr. The rootstock does not influence the composition of the vine leaf for the studied elements that are the most important in determining the geochemical footprint of the soil. The influence of the soil can be discriminated according to some elements such as Fe, Pb, Si and, especially, Sr.