Terroir 2020 banner
IVES 9 IVES Conference Series 9 Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Intraregional profiles of varietal thiols and precursors in Sauvignon Blanc juices and wines from the Adelaide Hills

Abstract

Aims: To investigate the intraregional variation of varietal thiol precursors and free thiols in Sauvignon blanc grape juices and experimental wines arising from the Adelaide Hills Geographical Indication (GI) in South Australia.

Methods and ResultsVitis vinifera L. cv Sauvignon blanc grape parcels (n = 21, approx. 8 kg each, encompassing 5 clones) were hand harvested from different blocks within seven commercial vineyards in the Adelaide Hills GI during the 2018 vintage. Parcels were divided into subsets for winemaking and freezing experiments. Amino acid (AA) and thiol precursor concentrations in juice were determined using high performance liquid chromatography (HPLC) with fluorescence detection and stable isotope dilution assay (SIDA) using HPLC with tandem mass spectrometry (MS/MS), respectively, and free thiols in wine were quantified by SIDA with HPLC-MS/MS, after derivatisation with 4,4’-dithiodipyridine. Intraregional variations in grape ripeness were evident according to total soluble solids content, pH, and titratable acidity, even within single locations or for the same clones. Significant differences in the glutathionylated precursor to 3-sulfanylhexan-1-ol (3-SH) were found among several locations whereas for the cysteinylated variant of 3-SH, one location was distinct from the rest. Variation in precursor concentrations was also noted from different blocks within a single vineyard location but was not dependent on grape ripeness. Fermentations progressed without any obvious relationship to location, and wines that were high in 3-SH were also usually high in 3-sulfanylhexyl acetate (3-SHA). One location had significantly higher levels of thiols in wine despite the juice not being the highest for grape-derived precursors, and also gave a substantial concentration of 4-methyl-4-sulfanylpentan-2-one in comparison to other locations within the GI. The AA profile of juices was found to vary according to location, and certain AAs were strongly correlated to thiol precursor concentrations, but relationships of AAs with free thiols in wine were generally weak. Additionally, enhancements in the concentrations of precursors in juice (up to 19-fold) and free thiols in wine (up to 10-fold) were revealed from freezing whole grape bunches in contrast to using fresh juice.

Conclusions: 

Intraregional variation was noted for thiols in wine, and precursors and amino acids in juice, for 21 Sauvignon blanc samples collected from within the Adelaide Hills region. The effects of terroir were implicated in explaining the differences in grape composition, and the potential interactions among grape amino acids and thiol precursors in berries and thiols in wine were revealed.

Significance and Impact of the Study: Sauvignon blanc is a significant variety produced in the Adelaide Hills GI but no information was available on the effects of location within the GI on grape and wine composition with respect to varietal thiols. This was the first study of intraregional variations of thiol precursors, amino acids, and free thiols in Sauvignon blanc juices and wines that were produced in a consistent manner. A remarkable enhancing effect of freezing was noted for thiol precursors in juice, and importantly, free thiols in wine.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Liang Chen1,a, Dimitra L. Capone1,2, Emily L. Nicholson3, David W. Jeffery1,2*

1 School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1 Glen Osmond, SA 5064, Australia
2 Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, PMB 1 Glen Osmond, SA 5064, Australia
3 CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA 5064, Australia

aPresent address: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux, 210 chemin de Leysotte CS 50008, 33882 Villenave d’Ornon Cedex, France

Contact the author

Keywords

List of different keywords (keyword1, keyword2, keyword3)

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Climate change impacts on Douro Region viticulture and adaptation measures

Climate has a significant impact in the success of any agricultural system, with a direct influence on the crops suitability to a given region, interfering on yield and quality and also with the economic sustainability of the productive activity. In the Douro Demarcated Region (RDD), as in most regions of the Mediterranean climate, the scarce precipitation (33% has less than 600 mm per year), and your high variability, associated with high rates of evapotranspiration during the summer, is usually one of the fundamental factors that limit the grapevine development, as well as the production and quality of the harvest. Thus, facing the scenario in temperature changes for the next decades (1.5-2.5°C) and confirming the predictions of precipitation decreases and/or great variability in the occurrence of heat waves and intense rainfall, the consequences for slope stability in mountain viticulture and sustainability of all operations involved, are risks to be taken into account. In this way, a deepest and sustained knowledge regarding the adaptation measures to adverse environmental conditions is of a crucial importance, enabling a more efficient adaptation of plant growth conditions and the optimization of production and quality of the grapevines. The development of this work, carried out in two commercial vineyards, one located in Soutelo do Douro, São João da Pesqueira, Cima Corgo sub-region, and another located in Numão, Vila Nova de Foz Côa, Douro Superior sub-region, it seeks to establish a relationship between climatic elements and physiological, productive and qualitative parameters, as well as to evaluate the effectiveness of adaptation measures, including different types of deficit irrigation (2002-2019) and the application of shading nets (2019-2020) in the physiological, viticultural and oenological behavior in the Touriga Nacional and Moscatel Galego Branco varieties, respectively. The results showed that the application of deficit irrigation allowed to significantly reduce the impact of the adverse weather conditions at key moments in the development of the grapevine, particularly in the period immediately before veráison and maturation, reducing the negative effects on the physiological processes and productivity, without compromise the must quality parameters. On the other hand, the application of shading nets significantly reduced de leaves temperature, allowing to increase the water potential, stomatal conductance and photosynthetic rate of grapes, which was reflected in the yield increase in the 2nd year of the study. For the maturation indicators, higher levels of total acidity, malic acid and assimilable nitrogen were obtained. The last measure presents a huge potential, being essential to carry out more years of trials to obtain stronger conclusions in terms of production parameters, but also in characteristics as important as the grape ripening components and the organoleptic characteristics of wines.

Sustainable fertilisation of the vineyard in Galicia (Spain)

Excessive fertilization of the vineyard leads to low quality grapes, increased costs and a negative impact on the environment. In order to establish an integrated management system aimed at a sustainable fertilization of the vineyards, nutritional reference levels were established. For this purpose, 30 representative vineyards of the Albariño variety were studied, in which soil and petiole analyses were carried out for two years and grape yield and quality at harvest were measured. In both years of study, soil pH, calcium, sodium and cation exchange capacity were positively correlated with calcium content and negatively correlated with manganese in grapes. Irrigated vineyards had higher levels of aluminium in soil and lower levels of calcium in petiole. Climatic conditions were very different in the years of the study. The year 2019 was colder than usual, in 2020 there was a marked water stress with high summer temperatures. This resulted in medium-high acidity in grapes in 2019 and low acidity in 2020, with sugar levels being similar both years. A very marked decrease in must amino nitrogen was observed in 2020, with ammonia nitrogen remaining stable. The correlation of acidity and sugar values in grapes with soil and petiole analysis data made it possible to establish reference levels for the nutritional diagnosis of the Albariño variety in this region. Based on these results, an easy-to-use TIC application is currently being created for grapegrowers, aimed at improving the sustainability of the vineyard through reasoned fertilization. This study has now been extended to other Galician vine varieties.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.