Microbial metagenomics of vineyard soils and wine terroir

In northern cold climate conditions, chilling requirement fulfillment in dormant grapevine buds is slowed or stopped by subzero temperatures impacting the transcriptional processes needed to complete chilling requirement. Cabernet Franc and Reisling in Geneva, NY were used to determine the impact of natural oscillating temperatures on grapevine bud transcriptional activity during light and dark periods of a two-week period in January with fluctuating diurnal winter temperatures. Cabernet Franc and Reisling bud samples were collected at 32 time points during the natural vineyard temperature cycle at 6:00 (dark), 14:00 (light) and 18:00 (dark) hours) to monitor gene expression in consecutive freezing and non-freezing temperature oscillations. Genotype, light and dark, and temperature oscillations conditions were explored.

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.

Wine quality depends on grape biochemical constituents, including sugars, organic acids, amino acids, and bound and free aroma compounds, which are influenced by vineyard location and environmental factors such as temperature and precipitation [1].

This experiment tries to characterize the role of soil, climate and harvest date on the composition of grape-derivated thiols, 3-mercapto-hexanol (3MH) and 3-mercapto-hexile acetate (A3MH), in the white wines from Colombard varieties in Gascony (South-West of France). A network of 6 plots has been observed since 1999 on different pedologic units. The plots have common agronomical characteristics, plantation spacing (2,900 to 3,500 vines per ha), plantation aging (1985-1990), strength conferred by rootstock (SO4, RSB), soil management (grass covered 1 by 2) and training system (vertical shoot positionning pruned in single Guyot). Meteorological stations are located near the plots.

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.