Usefulness and limits of the crop water stress index obtained from leaf temperature for vine water status monitoring

The objective of the present work was to study the influence of the foliar application of seaweed (Laminaria japonica), on the bunch and on the must in the ‘Cabernet Sauvignon’ grape. The experiment was carried out in the years 2021/2022, in a 21-year-old commercial vineyard, in the municipality of “Dom Pedrito” – “Rio Grande do Sul” (RS). A completely randomized experimental design was used, with 4 treatments and 4 replications (7 plants per replication). The treatments were: T1- control treatment; T2- Exal Powder 5 g L-1; T3- Hidro Exal 15 ml L-1; T4- Exal Powder 5 g L-1+ Hidro Exal 15 ml L-1.

Aerial thermography has emerged as a promising tool for water stress detection in grapevines, but there are still challenges associated with this technology, particularly concerning the methodology employed to extract reliable canopy temperature values. This consideration is relevant especially in vertically trained vineyards, due to the presence of multiple surfaces which are captured by drone thermal cameras with high-resolution. To test the technology and the data analysis required, a field study was conducted during the 2022-2023 season in a model vineyard with multiple scions-rootstock combinations trained on a vertical shoot-positioning (VSP) system. Additionally, three irrigation regimes were implemented to introduce variability in water stress levels.

Teran is an important native variety grown in Istria known for its high level of polyphenols and intensive fruity character of wines. Teran’s yield and wine typicity have recently decreased due to climate changes (increased temperature and severe drought). Four drip irrigation treatments (25%, 50%, 75%, 100% of total evapotranspiration) and control were investigated for the influence on Teran yield and quality, where focus was given to the content and composition of main polyphenolic and volatile compounds in grapes. Irrigation positively influenced yield since the berry weight also increased with increased irrigation. This resulted in the highest yield for 100% ETc. The highest concentration of polyphenols had control, while the irrigation treatments did not differ significantly. However, there was a tendency to decrease concentration with increased irrigation probably due to the increased berry size, which led to a dilution effect. Regarding the volatile compounds, the most abundant group was alcohols, followed by acids.

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.

All techniques usually used to assay proteins was not reliable in vegetable extract due to interferences with the components included in extracts like polyphenols, tanins, pectines, aromatics compounds. Absorbance at 280nm, Kjeldhal assay, Biuret and Lowry methods, Acid Bicinchonique technique and Bradford assay give the results depending on the composition of extract, on the presence or not of detergent and on the raw material (Marchal, 1995). Another difficulty in these extracts for the quantification of proteins comes from the large amount of water included in vegetable and the low concentration of proteins. Thus in red wines, proteins are usually not taken into account due to their low concentration (typically below 10 mgL-1) and to the presence of anthocyanis and polyphenols.